protein delivery: dna nanostructures and cell-surface targeting harvard igem august 27, 2006
TRANSCRIPT
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Protein delivery: DNA nanostructures and cell-surface targeting
Harvard iGEMAugust 27, 2006
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The Machine Goal: Future modular drug delivery
drug
target cell
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Molecular containers in nature Hard to duplicate artificially
http://micro.magnet.fsu.edu/cells/viruses/images/virus.jpghttp://www.biology4kids.com/files/art/cell_over1.gif
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DNA Nanostructures Overview
DNA can be used to approximate arbitrary 3D structures
WILLIAM M. SHIH, JOEL D. QUISPE & GERALDF. JOYCENature 427, 618 ミ 621 (2004);
http://www.dna.caltech.edu/~pwkr/
William Shih, Harvard Ned Seeman, NYU Paul Rothemund, Caltech
http://seemanlab4.chem.nyu.edu/nano-cube.html
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Motivation: Why DNA?
The power of DNA Nanometer scale Covalent modifications possible Inexpensive synthesis Highly programmable/designable
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Design Details: Scaffolded Oragami
M13 viral genome
7308 bases long
Add ~180 helper strands in Mg++ buffer
Heat to near boiling. (90 C)
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Design Details: Scaffolded Oragami
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Design Details: Scaffolded Oragami
When the sample reaches room temperature (2hrs later), the origami have folded
http://www.dna.caltech.edu/~pwkr/
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Design Details: Positional Control
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Design DetailsDouble-ply barrel and lid
Lid: 33 nm across, 28 nm longBarrel: 27.5 nm long, 27.6 nm across
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Exciting EM Images
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Exciting EM Images
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To be continued
Can a protein be protected from protease if attached inside the box?
Lid attachment Lid removal
protein
protein
protease
protease
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Acknowledgements
Harvard TFs - Shawn Douglas, Nick Stroustrup, Chris Doucette
Harvard advisers - Dr. William Shih, Dr. George Church, Dr. Pamela Silver, Dr. Alain Viel, Dr. Jagesh Shah, Dr. Radhika Nagpal
iGEM ambassadors iGEM directors