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Ciclo celular de procariotas I

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Ciclo

celular de procariotas II

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La tcnica baby-machine

Exponentially growing culture lB

I C 101Period

Elutiontime O0.25 0.5 0.75 1Cellage

Fiq. 22.2a-c Th~membrane-elution tech-nique. .a An exponentially growingpopulation of

Membrane

(generations

O 0000

!

Mernbranesurface

bacteria is rapidly c"#lled andt"en passedt"roug" a nitrocellulosefilter$ %"en asufficient number of cells

"a&e been collected on t"e filter$ t"efilter de&ice is

upport0.25

00000!roplets

in&erted$ and prewarmedfres" culture me diumispassedt"roug" t"e filter$'"e bacteria remainattac"ed to t"e filter andgrow on its

ofwaste

0.5

medium

00

0

00

surface$ %"en a celldi&ides$ one of t"e

daug"ter cells (newborn cellcan be eluted

..

0.75

In&ert filter de&iceb

!Elutewit"

c(() 1.0fres" medium )(i*

and eit"er collected andc"illed as starting material$for a sync"ronous cultureor col lected o&er time infractions for analysis$b '"e exponentialpopulation consists of cellsof different ages$ .ewborn

cells are of age O. and cellst"at are finis"ingcell di&isionare of age 1. '"ese arerepresented on an enlarged

..

!

* !

..

0000

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imirnt

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!ecimiento de la

membana

% 5

!

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Crecimiento de la pared

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Crecimiento de la pared de cocos 7ram 8

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9l cluster de :enes de di"ision

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9l replisoma

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"e7aacin de lo* nucleoide* hi;o*

5a;a 9elocidad de cecimiento

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%lta 9elocidad de cecimiento

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"e8e8acin de lo* nucleoide*

&ote

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&otee en 7ocaiota*

%ctina =e5 &a=

!itoe*?ueleto

eucaitico

@oma celula

7ocaiota*

Re7ato %A

7ocaiota*

@ilamento* &a= enE. coli !able* =e5 en clula* bacilae*

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epto de di"isi;n

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FTs< = anillo

ecuatorial

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@t*K

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Reclutamiento de ota* 7ote

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%nillo K

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Ki7% y @t*%

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+ede* et al. DEEF. 5acteial mitotic machineie*. !ell GGH, J4-H

he ob*e9ation* de*cibed heein e9eal that,

*u7i*in8ly, Aatue ha* cho*en to u*e actin and

tubulin fo eci7ocal 7u7o*e* in t/o diffeent

domain* of life: In mo*t eu>ayotic cell ty7e*, actini* a fundamental com7onent of the cyto>inetic in8

/hile tubulin fom* the mitotic *7indle fibe*. In

conta*t, in bacteia, the ubi?uitou* tubulin-li>e

@t*K 7otein fom* the cyto>inetic in8, /hile the

actin-li>e 7otein &a= (and 7o**ibly =e5M fom*

a mitotic-li>e machiney that *e8e8ate* A%.

Locali6acin ecuatoial del *e7to

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Locali6acin ecuatoial del *e7to

il i d l

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*cilacin del

*i*tema =in!B

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=in!

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=in

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eteminacin de la 7o*icin

del *e7to

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! i li i di i i

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Tiempo de

:eneraci;n0minutos-

>0

50

?0

&0

!oneNin ente e7licacin y di9i*in

Ciclo celular 0minutos-

0 10 20 &0 ?0 50 >0

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Bfecto de di9e*a* mutacione* (E. coliM

dnaA dnaE

minC,D,E gyrB

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envA ftsI,Q,Z

ftsA