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    Duckweed

    - A sustainable proteinsupplement for the future

    Microalgae

    - Different types of algaeprovide vital nutrients torotifers, copepods and larvaeof finfish, shellfish and shrimp

    Catfish productionin India:

    - Present status and prospects

    Pea Seed proteinconcentrate inTilapia feeds

    VOLUME 18 ISSUE 5 - SEPTEMBER | OCTOBER 2015

    AQUANOR 2015 review

    I N C O R P O R A T I N G

    F I S H FA R M I N G T E C H N O L O G Y

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    Editor

    Professor Simon Davies

    Email: [email protected] 

    Associate Editors

    Dr Albert Tacon

    Email: [email protected] 

    Dr Yu Yu

    Email: [email protected] 

    Dr Kangsen Mai (Chinese edition)

    Email: [email protected] 

    Editorial Advisory Panel• Abdel-Fattah M. El-Sayed (Egypt)

    • Dr Alber t Tacon (USA)

    • Professor António Gouveia (Portugal)

    • Professor Charles Bai (Korea)

    • Colin Mair (UK)

    • Dr Daniel Merrifield (UK)

    • Dr Dominique Bureau (Canada)

    • Dr Elizabeth Sweetman (Greece)

    • Dr Kim Jauncey (UK)

    • Eric De Muylder (Belgium)

    • Dr Pedro Encarnação (Singapore)

    • Dr Mohammad R Hasan (Italy)

    Editorial teamOlivia Holden

    Email: [email protected] 

    Malachi Stone

    Email: [email protected] 

    Peter Parker

    Email: [email protected] 

    Editor - Asia Pacific

    Roy Palmer

    Email: [email protected]

    Publisher

    Roger Gilbert

    Email: [email protected]

    Circulation & Events Manager

    Tuti Tan

    Email: [email protected] 

    Design Manager

     James Taylor

    Email: [email protected] 

    International marketing team (UK)

    Darren Parris

    Email: [email protected] 

    Tom Blacker

    Email: [email protected] 

    Latin America

    Ivàn Marquetti

    Email: [email protected]

    Pablo Porcel de Peralta

    Email: [email protected]

    India

    Ritu Kala

    Email: [email protected]

    Africa

    Nathan Nwosu

    Email: [email protected]

    More information:

    International Aquafeed

    7 St George's Terrace, St James' Square

    Cheltenham, GL50 3PT, United Kingdom

    Tel: +44 1242 267706

    Website: www.aquafeed.co.uk 

    CONTENTSVolume 18 / Issue 5 / September-October 2015 / © Copyright Perendale Publishers Ltd 2015 / All rights reserved

    International Aquafeed is published six times a year by Perendale Publishers Ltd of the United Kingdom.

     All data is published in good faith, based on information received, and while every care is taken to prevent

    inaccuracies, the publishers accept no liability for any errors or omissions or for the consequences of action taken

    on the basis of information published. ©Copyright 2015 Perendale Publishers Ltd. All rights reserved. No part of

    this publication may be reproduced in any form or by any means without prior permission of the copyright owner.

    Printed by Perendale Publishers Ltd. ISSN: 1464-0058

    REGULAR ITEMS

    3-15 INDUSTRY NEWS

    6 THE AQUACULTURISTS

    28 PHOTOSHOOT

    38 EXPERT TOPIC - CARP

    44 INDUSTRY EVENTS52 THE MARKET PLACE

    54 THE AQUAFEED INTERVIEW

    56 INDUSTRY FACES

    FISH FARMING TECHNOLOGYSUPPLEMENT

    002 The AKVA group automatic feedbarge - the Wavemaster AC850Panorama

    FEATURES

    12 Pea Seed protein concentrate inTilapia feeds

    16 Catfish production in India:Present status and prospects

    18 Growth trial and challenge testingfacility opens in Thailand

    22 Dusckweed - a sustainable proteinsupplement for the future

    30 Microalgae - Different types ofalgae provide vital nutrients torotifers, copepods and larvae offinfish, shellfish and shrimp

    36 Microalgae - Q&A with ReedMariculture Inc founder andPresident Tim Reed

    EXPERT TOPIC - KRILL

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     Meet the Aquafeed team at upcoming international events

    The season is certainly changing rapidly and autumnalweather is upon us here in the UK with much rain

    and threatening grey skies rolling in from the

    Atlantic. The nights are certainly drawing in and

    it’s now time for some of us in the UK to stock up and

    close the hatches.

    Plymouth, England is subject to typical ever changing

    maritime weather and it makes for interesting challenges.

    As a Welshman though, I am quite used to rain and storms

    whether they be climatic or even political (especially in aca-

    demia), and we usually find a song or a pub for solace and a

    place to think with a drink!It is only a step away from Christmas and reminders of the

    festive holidays are already in the shops with advertisements

    for food, drinks and all manner of decorations. I can’t wait!!

    Increasingly salmon is now presented along with the

     traditional turkey and other delights and the consumer has

    so much choice at hand in terms of products with various

     types of presentation from whole fresh salmon, fillets, smoked

    forms, pates and salmon encased in pastry, bread crumbs and

    in fish pies and salmon fish cakes etc.

    Aquaculture of course is at the core of production and one

    forgets that it is central to the supply chain of fresh salmon

    or chilled salmon products in our stores and supermarkets.

    Retailers are able to set specification requirements for fish at

    source and are important in their discerning of quality criteria

    and expectations from the consumer for a good reliable and

    nutritious product. As such, there is now increasing demand

    for salmon and with it a need for bespoken fish feed to sup-

    port the growth of salmon, promote excellent health in rela-

     tion to welfare and of course guarantee that the fish supplies

     the Omega-3 fatty acids (or oils, fats for public perception)

    for human health and well-being. Fish oils and fish meals are a

    finite resource and we must use them very strategically as fish

    farming of carnivorous and especially marine species expands

    globally.

    The medical research fraternity advocating that we should

    ideally consume 1-3 portions per week of salmon for opti-

    mum cardiovascular function, improved cognition, brain health

    and prevention of strokes is well known and is mentionedfrequently in the TV media and press. However there has

    been much concern that the replacement of marine derived,

    principally fish oils and fish meals in diets for salmon and other

    fish species too has caused a slow trend that could lower

    in some instances the Omega- 3 ratio to other fatty acids

    (Omega-6 series) in the diet and hence in fish such as salmon

     to a worrying threshold. This is not the absolute case as

    yet but significant differences in the Omega-3 levels do exist

    and certainly between wild Pacific salmon and wild/ farmed

    Atlantic salmon. One of the criticisms of the latter is their

     typically very high fat content and this is not appealing to some

    people with some adverse comments against the farming ofsalmon. However many have not even tasted the rather drier

    wild salmon, so it’s a matter of perception and taste.

    There is now a major new initiative launched by the

    Global Salmon Initiative (GSI) to encourage the develop-

    ment of novel Omega-3 alternatives to marine oil sources in

    attaining the sustainable agenda for salmon production and

    yet also satisfy the demands by the industry for Omega-3

    enriched fish.

    It is stated by GSI that ‘the farmed salmon industry uses

    approximately 350000 tons of fish oil each year, and this

    Creoso - welcome

    Professor Simon Davies

    16-19 November 2015

     LAQUA15, Brazil

    20-23 October 2015

     Aquaculture Europe 2015

    The Netherlands,

    Stand number 1.6 

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    What’s best forCardiovascularDiseases?Ioannis Zabetakis, assistant professor of food chemistry,University of Athens, Greece

    In my May/June article, some of the latest data on the functionality of

    statins and fish lipids against Cardiovascular Diseases (CVDs) were

    given. The story goes on and some related developments are given

    below.

    In the US, an FDA advisory panel has voted at the beginning of June

     to recommend approval of two new injectable cholesterol-lowering

    drugs that work differently than statins. These two drugs, Praluent by

     the drug company Sanofi and Repatha from Amgen, are a class of drugs

    known as PCSK9 inhibitors. These drugs block the PCSK9 protein in

     the blood, which allows the body to more effectively reduce the levels

    of LDL cholesterol.

    Two articles claiming cholesterol-reducing statins may be unsafe are to be investigated and could be retracted by the British Medical Journal.

    The authors have withdrawn figures suggesting up to 20 percent of

    users would suffer harmful side effects such as liver disease and kidney

    problems.

    Given that about seven million people in the UK at risk of heart

    disease are prescribed statins, experts fear the articles, which were

    widely reported in October 2014, will have discouraged people from

     taking them. British Medical Journal (BMJ) editor-in-chief Dr Fiona

    Godlee said last May it was publicising the withdrawal of the side-

    effects figures "so that patients who could benefit from statins are not

    wrongly deterred from starting or continuing treatment because of

    exaggerated concerns over side effects". But the scientific question: howsevere are the real side effects of statins?

    On the other front, for example, the one of consuming fish instead of

    statins, the news is encouraging:

    Eating fatty fish, such as salmon, herring and mackerel, at least three to

    four times a week has been shown to boost levels of high-density lipo-

    protein (HDL) cholesterol and lessen the risk for cardiovascular disease,

    according to a study published in the journal PLoS One. [http://journals.

    plos.org/plosone/article?id=10.1371/journal.pone.0090352].

    In a relevant recent research announcement, Australian researchers

    have found [http://www.abc.net.au/news/2015-04-13/fish-oil-not-pro-

     tective-against-heart-disease-study-finds/6389126] that fish oil supple-

    ments do not protect against heart disease with the evidence sug-

    gesting that eating fish is of greater benefit to the heart. Researchersexamined the benefit of fish oil supplements for the hearts of healthy

    people and those who have had a heart attack and are taking the sup-

    plement to prevent further episodes.

    The study, which has been published in the Heart, Lung and

    Circulation Journal, has prompted the National Heart Foundation to

    review its guidelines on fish and fish oil supplements. The Foundation

    said it shows higher fish intake is consistently associated with lower

    rates of sudden cardiac death, stroke, heart failure and heart attack.

    It is urging all Australians to eat two to three servings of fish a week,

    including oily fish.

    The evidence is clear and the news for the Aquaculture society

    is rosy! Eating fish protects better against CVDs (and without sideeffects) than any current drug at the moment! Plus, fish has a pleasant

    flavour!

    [email protected] 

     @yanzabet 

    demand is expected to grow by approximately five

    percent each year in line with industry growth’.

    This challenge is already being met by several biotech-

    nology companies and a few scientific papers are now

    available demonstrating the feasibility of plants, algae

    and yeast as sources of EPA and DHA Omega-3’s that

    can effectively be retained to enhance the salmon flesh

    levels at harvest. The call is for companies to provide a

    significant increase in availability of such materials over a

    specified time period of at least two years. This I think

    will be a most exciting course of action and I believe will

    open new opportunities for discovery, innovation and

    enterprise in aquaculture. It will be one of those decisive

    markers affecting costs, competitiveness and overall suc-

    cess of an industry so crucial to Scotland, Norway, Chile,

    Iceland, and beyond.

    Now in this issue of IAF we have our special expert

    feature focusing on Krill and as a growth accelerator

    with its numerous nutritional attributes especially those

    Omega-3’s and the powerful anti-oxidant astaxanthinwell known for its flesh colouring properties for salmonid

    fish.

    My colleague Dr Ingrid Luputsch in her new role

    at AB-Agri reports on the use of pea seed protein

    concentrate in experimental diets for tilapia, something I

    have worked on over the years with Professor Antonio

    Gouveia in Portugal for many fish species and with

    robust outcomes for inclusion in fish feeds.

    Our special fish interest in this issue is catfish of

     the Asian variety (There is much potential in Claridi

    spp., and its excellent eating too, we worked for over

    a decade on this tropical species when I was basedat Plymouth University, and bred several thousand in

     the heart of the city!) The article by Dr B. Laxmappa,

    Fisheries Development Officer, Department of Fisheries,

    Telangana, India discusses Ictalurus Silurus, pangasius and

    Clarias gariepinus species generically called catfish in rela-

     tion to their production and contribution to freshwater

    aquaculture in India.

    In some forms of aquaculture, aquatic vegetation

    makes a valuable feed contribution to less intensive types

    of production systems and so duckweed is discussed in

    a technical/ scientific report. However its processing can

    generate an invaluable high protein concentrate for fish,

     terrestrial farm animal nutrition and for human nutritionapplications making duckweed an added value product

    of significant potential.

     We certainly need more trained aquaculture fish

    health specialists and I am pleased that we include news

    of the opening of the new Fish Vet Asia laboratories at.

    Chonburi, 90 minutes south-east of Bangkok.

    Finally our events section addresses Aquanor and

    previewing the EAS & Aquaculture Europe meeting for

    October.

    There is a diversity of news items and regular column

    writers and so I wish you good reading and a busy and

    successful autumn in your business endeavours.Professor Simon Davies

    September-October 2015 | INTERNATIONAL AQUAFEED | 3

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    Nutriad sponsors

    The Aquaculture

    Roundtable Series

    (TARS) 2015

    Nu t r i a d w a s a

    proud sponsor of

    'The Aquacu l tu re

    Roundtable Series (TARS) 2015,'

    held on September 19-20 in

    Hanoi, Vietnam. The central theme

    of this fifth edition of TARS was

    'from farm to plate' and focused

    on new approaches to improve

    feeds and feeding in aquaculture.

    The event attracted around 200

    participants representing major

    aquafeed mills, big farm owners

    and major suppliers of the Asian

    aquaculture industry.

    TARS includes a ser ies of round-

     table sessions where stakeholdersfrom industry, academia, govern-

    ments and NGOs convene to

    share ideas and identify strate-

    gies to ensure sustainable devel-

    opment of Asia’s aquacul-

     ture industr y. This year, par tici -

    pants had fruitful discussions in

    breakout sessions covering four

     themes: Feeding Today's Shrimp;

    Freshwater Fish Feeds; Marine

    Fish Feeds; and the Aquafeed

    Industry.

    G loba l Aquacu l ture BU

    manager Dr Peter Coutteau pre-

    sented a talk titled 'Towards a

    paradigm shift in feed specifica-

     tions,' comparing feed regulations

    for aquafeed in different Asian

    countries and those in force in

     the EU. Feed standards based on

    simple analytical features are not

    effective and risk limiting inno-

    vation which is badly needed to

    face current industry challenges.

    Up-to-date aquafeed includes

    nutrient quality and functional

    properties to improve feed utili-

    sation and animal health but also

    requires adequate information

    (labelling) and continued educa-

     tion and suppor t of the farmer.The aquaculture industry would

    benefit more from regulations

     target ing feed and food safety

    rather than feed specifications,

    and would further benefit from

    uniformity of regulations across

     the ASEAN region. Dr Coutteau

    received a lot of feedback from

     the feed mill industry, which hasbeen limited by national regula-

     tions for many years.

    The Nutriad Gala Dinner,

    organised at the Marriott Hotel

    in Hanoi, was an excellent oppor-

     tunity for visiting aquafeed profes-

    sionals and Nutriad’s expert team

     to further discuss on impor tant

     topics while enjoying good foodin pleasant surroundings.

    Nutriad delivers products and

    services to over 80 countries

     through a network of its own

    sales offices and distributors, sup-

    ported by four application labora-

     tories and five manufacturing facil-

    ities on three continents.

    From left: Hai Diep, Area Manager IndoChina; Gim Chong Ho,Commercial/Technical Manager Aquaculture SE Asia; Alexander

    van Halteren, Business Development Manager AquacultureAsia-Pacic (AP) and Europe, Middle East & Africa (EMEA); Peter

    Coutteau, Business Unit Manager Aqua Additives; Allen Wu, RegionalAquaculture Manager, SE Asia

    Biomin opens new

    Panama plant

    Biomin has further expanded its global

    production network with the opening

    of a production plant in Panama. The

    inauguration marks the latest milestone in

     the firm’s local presence that stretches back

    nearly two decades. The new plant will allow

    for growth and quicker delivery to Biomin dis-

     tributors and customers in new and existing

    markets throughout the Americas.

    The plant produces Mycofix®, an innova-

     tive mycotoxin deactivating feed additive, with

    plans to produce PoultryStar®, a multi-species

    probiotic for poultry, in the future.The site was planned with a long-term per-

    spective and an expectation of growth in

    mind. It has an installed production capacity

    of 3120 tons per year based on one pack-

    aging station and a single shift. Its starting pro-

    duction goal is 6240 tons per year with two

    shifts. Maximum production capacity can reach

    12,480 tons per year with the addition of a

    second packaging station.

    On August 20, 2015 Christian Seiwald,

    Chairman of Erber AG, and Esteban Giron,

    Vice Minister of Agricultural Development

     joined in the cutting of the ribbon inaugu-

    rating the plant in Panama alongside BiominManaging Director, Marcelo Ribeiro, marking

    a momentous occasion for the company and

     the region.

    “A strong, longstanding commitment to sci-

    entific research has allowed Biomin to deliver

    leading products in an ever-increasing number

    of markets across the globe,” commented Mr

    Seiwald.

    Also in attendance were Ruben Beltran,

    Managing Director of Biomin USA along with

    50 invited guests including key distributors and

    customers from throughout the region.

    Aquaculture UK under

    new management

    Ascomber Ltd, the Scottish-based

    Event Company and organisers

    of Aquaculture UK was recently

    acquired by 5m Publishing Ltd.

    Ascomber diversifies the 5m Aquaculture

    portfolio and compliments ‘The Fish Site’ and

    our educational MSc and related post-grad-uate courses delivered in partnership with the

    University of St Andrews.

    5m Publishing publishes online topics

    such as The Poultry, Avicola, Pig, Porcino,

    Fish, Beef, Diary, Cattle, Meat, Crop, Pet,

    AgriTimes and Sheep sites.

    Now in its tenth year, Aquaculture UK is the

    most important aquaculture exhibition and

    conference held in the British Isles.

    Although the ownership will change, the

    name will remain the same and International

    Aquafeed will continue to serve you under

    Aquaculture UK.

    David Mack, founder of the event, remains in

    an advisory role to ensure a smooth transition

    and a successful 2016 event!

    You can reach the new team which com-

    prises:

    Commercial: Matt Colvan at

    [email protected]

    Marketing: Nicola Pickles [email protected]

    Accounts: Sam Baker at

    [email protected]

    All visitor enquiries to Helena Thompson at

    [email protected]

    September-October 2015 | INTERNATIONAL AQUAFEED | 5

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    IKEA makes responsibly produced

    seafood available to over 600

    million customers

    IKEA today announces that seafood

    sold and served in its stores comes

    from sustainable and responsible

    sources. The IKEA commitment toASC and MSC certified seafood

    marks the next step towards a

    wider offer of healthier and more

    sustainable food.

    http://bit.ly/1V95pUo

    Oceana wins landmark decision

    that grants access to antibiotics

    used by salmon farms in Chile

    In a unanimous ruling, Santiago’s

    Court of Appeals upheld the ille-

    gality claim submitted by themarine conservation organisation,

    Oceana, and demanded disaggre-

    gated information at salmon com-

    pany-level about the amounts and

     types of antibiotics used between

    2009 and 2013, figures which were

    denied to the NGO by the Chilean

    Transparency Council last April.

    http://bit.ly/1NSJnkC

    New Biomin video: mycotoxin

    risk management

    Mycotoxins cost the l ive-stock industry billions each year.

    Harnessing the power of science,

     the fifth generation of Mycofix®

    unites three strategies to combat

    mycotoxins using patented pro-

    prietary technology, making it the

    most scientifically advanced myco-

     toxin protection available.

    http://bit.ly/1KAfy27

    A regular look inside

    the aquaculture

    industry

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    www.theaquaculturists.blogspot.com

    GePro celebrates 50th anniversary in style

    F

    ood in the Change - was the theme of

    GePro's 50th anniversary celebrations,

    which took place in the international city of

    Hamburg.The program consisted of some seven presen-

     tations, which turned out to be more of a lecture

    format from a wide spectrum of professorships from

    Germany, the Netherlands to Israel and beyond.

    In fact, the subject matter was equally diverse

    and not just focused on poultry production and

    processing, which is at the centre of GePro's range

    of feed industry products.

    Bernd Grosse Holthaus, the company’s CEO

    and sales director welcomed some 80-plus invited

    delegates to the rooftop conference room that

    offered views over Hamburg and its harbour.

    National television presenter Sabine Stamm

    moderated the whole day's program, expertly

    weaving the presentations together despite their

    diverse nature.From the need for modern agriculture, a review

    of poultry production in Europe to pet food devel-

    opments and farming fish in the desert and other

     topics of Africa; from cultured meat to a novel

    approach we should take to sustainability; all capti-

    vated an appreciative audience's imagination.

    The company hosted its gala dinner for over 300

    company personnel and invited customers, sup-

    pliers and international press on the Saturday in

    Steinfeld in the heartland of Germany's livestock

    production region and near its factory north of

    Hamburg.

    Satellite technology puts

    ‘mussel’ into shellfish

    monitoring

    A team of UK scientists are exploring the

    use of satellites and meteorological data to

    monitor and forecast water quality events

     threatening shellfish farms, for the benefit of the

    shellfish industry and, ultimately, consumers.

    The farming or cultivation of seafood, termedaquaculture, is an important worldwide source of

    protein. As global populations continue to rise at

    approximately 1.13 percent a year, the expansion

    of aquaculture is considered key to help provide

    food security for future generations.

    In the UK shellfish farming generates £20-25

    million turnover each year, with worldwide

    demand for shellfish is anticipated to grow by five

    percent a year.

    This expected growth provides a clear business

    opportunity for UK shellfish farming. Tools that

    can provide environmental information directly tofarmers will help to continue safe, productive and

    sustainable aquaculture farming, whilst also helping

     the industr y to expand and meet the nutrition

    needs of future populations.

    Now, the two-year ShellEye project (www.shelleye.

    org), funded jointly by the UK Biotechnology and

    Biological Science Research Council (BBSRC) and

    Natural Environment Research Council (NERC),

    will be helping to advance shellfish farming man-

    agement practices. The project will initially focus

    on the development of novel satellite monitoring

    and short term forecasting techniques, which will

     then be integrated into the first water qual ity

    monitoring service for harmful algal blooms and

     targeted pollution events, specifically for the shell-

    fish aquaculture industry.Changes in water quality, such as the formation

    of harmful algal blooms, can have a negative impact

    upon shellfish farms and, in rare cases, can also

    pose a public health issue through the consump-

     tion of contaminated stock. Water quality in and

    around aquaculture farms in the UK and Europe

    is currently monitored by government agencies,

    using a series of tests based on collecting water

    samples and analysing the flesh of the seafood

    being farmed.

    Enhancing current monitoring practises, through

     the use satel li tes and weather forecasts , wil lprovide farmers with a cost-effective, near real-

     time source of information in the form of elec-

     tronic bul let ins , to help manage she llfi sh har-

    vesting. In turn, this should help minimise poten-

     tial health.

    6 | INTERNATIONAL AQUAFEED | September-October 2015

    EWS

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    Interestingly the example mentioned was in Nepal,

    a country currently suffering the enormous effects

    of a recent earthquake, Nepal was a country

    where AwF had helped establish aquaculture – see

    project reports 2008-2012. I particularly recall Ram

    Bhujel (AIT and AwF Volunteer) saying at the AwF

    Session during a WAS-APC Conference in Ho Chi

    Minh City, Vietnam, how important AwF had been

    in his birthplace (Nepal) in showing that aquacul-

     ture was possible and also empowering women to

    engage.

    As the US aid newsletter states, “In rural Nepal,

    widespread poverty is compounded by the lack of

    access to high-quality, nutritious foods.” According to

    a recent report from the Nepal Demographic Health

    Survey, 41 percent of children under the age of five

    are chronically malnourished, and anemia is a signifi-cant problem, afflicting 47 percent of children and 36

    percent of women.

    One approach to mitigate the occurrence of

    anemia and to improve the overall health of rural

    Nepalese is to supplement their diets with vitamin-

    rich protein sources, such as fish. Researchers from

    Nepal’s Agriculture and Forestry University (AFU)

    recognised the potential of aquaculture to help

    address this widespread nutritional deficit, and their

    recent effort in Nepal successfully established more

     than 70 family-run fishponds, all managed by women.

    In the first year of operation, the ponds producedover 500 kg of fish for household consumption.

    Looking back at AwF Project reports I read that

    an aquaculture awareness program was organised,

     this involved gathering a group of women and using

    computers from a higher secondary school in the

    village. A program produced on CD that was based

    on the “Women in Aquaculture Project” in Chitwan

    was shown, followed by questions and answers. Even

    at that stage organisers were reporting that a lot of

    women had shown their interest.

    From that report I read, “Altogether 52 families

    applied and showed interest in culturing fish on

     their land which was almost double the number theproject team had expected. Full technical support

    (training, field visit and fry supply) was offered to all

    of them, and a partial financial support was extended

     to all of them dividing them into two categories

    i.e. very poor and poor; with more support to the

    former.”

    According to US Aid, ‘Researchers intend to train

    more women in effective aquaculture techniques

    by establishing women’s groups to educate rural

    Nepalese on fish farming practices and the nutri-

     tional benefits associated with household fish pro-

    duction.Dr Thilsted highlighted that the characteristics of

    a healthy diet for the poor included natural foods

    which are locally produced; culturally acceptable

    (taste, texture, colour, flavour, etc); affordable; high in

    nutritional quality; safe and available year round.

    I t is claimed that as much as 47 percent

    of the edible US seafood supply is lost

    each year, mainly from consumer waste,

    according to latest research from the Johns

    Hopkins Center for a Liveable Future

    (CLF).

    The findings, to be published in the

    November issue of Global Environmental

    Change, come as food waste in general has

    been in the spotlight and concerns have

    been raised about the sustainability of the

    world’s seafood resources.

    In the US and around the world, peopleare being advised to eat more seafood to

    improve their health and wellbeing, however

    overfishing, climate change, pollution, habitat

    destruction and the use of fish for other

    purposes besides human consumption are

    potentially threatening supply.

    “If we’re told to eat significantly more

    seafood but the supply is severely threat-

    ened, it is critical and urgent to reduce

    waste of seafood,” says study leader David

    Love, PhD, a researcher with the Public

    Health and Sustainable Aquaculture projectat the CLF and an assistant scientist at the

     Johns Hopkins Bloomberg School of Public

    Health.

    The new study analysed the food waste

    issue by focusing on the amount of seafood

    lost annually at each stage of the food supply

    chain and at the consumer level.

    Data was compiled from many sources

    and from that, the researchers estimated the

    US edible seafood supply at approximately

    2.132 billion kg (4.7 billion pounds) per year,

    which includes domestic and imported prod-

    ucts minus any exported products.Some of the edible seafood supply is

    wasted as it moves through the supply chain

    from harvest to plate. They found that the

    amount wasted each year is roughly 1.04

    billion kg (2.3 billion pounds). Of that waste,

     they say that 150 million kg (330 million

    pounds) are lost in distribution and retail,

    260 million kg (573 million pounds) are lost

    when commercial fishers catch the wrong

    species of fish and then discard it (a concept

    called by-catch) and a staggering 590 mil-

    lion kg (1.3 billion pounds) are lost at theconsumer level.

    The researchers discovered the greatest

    portion of seafood loss occurred at the level

    of consumers (51 to 63 percent of waste).

     Whilst 16 to 32 percent of waste is due

     to by-catch and 13 to 16 percent is lost in

    distribution and retail operations.

    To illustrate the magnitude of the loss,

     the authors estimate this lost seafood could

    contain enough protein to fulfill the annual

    requirements for as many as 10 million men

    or 12 million women; and there is enough

    seafood lost to close 36 percent of the gap

    between current seafood consumption and

     the levels recommended by the 2010 US

    Dietary Guidelines.

    The 2010 US Dietary Guidelines recom-

    mended increasing seafood consumption to230g (eight ounces) per person per week

    and consuming a variety of seafood in place

    of some meat and poultry. Achieving those

    levels of demand would require doubling the

    US seafood supply, the researchers say.

     Waste reduction has the potential to

    support increased seafood consumption

    without further stressing aquatic resources,

    says Roni Neff, PhD, director of the Food

    System Sustainability and Public Health

    Program at CLF and an assistant professor

    with the Bloomberg School of Public Health.

    She says that while a portion of the loss

    could be recovered for human consumption,

    “we do not intend to suggest that all of it

    could or should become food for humans.

    “It would generally be preferable for the

    fish that becomes by-catch to be left alive in

     the water rather than eaten, and due to sea-

    food’s short shelf life, it may be particularly

    challenging compared to other food items

     to get the remaining seafood eaten or frozen

    before it decays,” she says.

    In the report the researchers offer several

    approaches to reduce seafood waste along the food chain from catch to consumer.

    Suggestions range from limiting the percent

    of by-catch that can be caught at the produc-

     tion level to packaging seafood into smaller

    portion sizes at the processing level to

    encouraging consumer purchases of frozen

    seafood.

    Some loss is unavoidable, but the research-

    ers hope these estimates and suggestions will

    help stimulate dialogue about the significance

    and magnitude of seafood loss.

    Note: “Wasted seafood in the UnitedStates: Quantifying loss from production to

    consumption and moving toward solutions”

    written by Dave C. Love, Jillian P. Fry, Michael

    C. Milli and Roni A. Neff is available at: http:// 

    bit.ly/1iJdMFc

    Seafood waste reduction sought- Researchers say waste adds to otherproblems threatening global seafoodresources

    Roy Palmer, director, Aquaculturewithout Frontiers

    Award for

    Columbian

    champion

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     Well-balanced multi-stakeholders’ participation is a key prin-

    ciple for GMP+ International’s operations in the market. It

    is an important strategy to involve and commit the stake-

    holders in the whole feed chain as well as following links in animal pro-

    duction, like livestock and aqua farming, dairy, meat and egg processing

    industry. GMP+ International’s partners are allowed to nominate can-

    didates for its expert committees in charge of defining the content of

     the GMP+ Feed Certification scheme.

     When you search on Google with the phrase ‘well-balanced multi-

    stakeholders participation’, it results in a limited number of hits, allrelated to GMP+ International. The pr inciple of ‘multi-stakeholders par-

     ticipation’ as such, results in more hits and is a better-known principle

    applied in the public sector.

    It is a conceptual model for participatory decision-making. It is based

    on the view that stakeholders, experts, and citizens could contribute to

    developing a wished outcome based on their particular expertise and

    experience as well as interests. The concept of this participation model

    is oriented toward a multi-actor, multi-value, and multi-interest situation.

    These groups represent three forms of knowledge:

    a. knowledge based on common sense and personal experience;

    b. knowledge based on technical expertise; and

    c. knowledge derived from social interest and advocacy.The principle of multi-stakeholders’ participation fits very well to

    GMP+ International’s core business related to feed safety and responsi-

    bility assurance, which are common interests of the feed and food pro-

    ducing industry. Obviously, there is also a public interest about it. It is

    based on two of GMP+ International’s core values: integrity and objec-

     tivity. These values are taken into account in case of the participatory

    decision-making process in combination with transparency about the

    pro-cess and final result. GMP+ International integrated the principle

    of multi-stakeholders’ participation in two ways: (i) partnership and (ii)

    public consultation.

    Partnership of stakeholder groups is aimed at involvement of the

    stakeholders in the feed and food chain in the decision-making regarding

     the content of the GMP+ Feed Certification scheme and the relatedintegrity policy. It is realised by participation of experts in the expert

    committees. It should result in suppor t of and commitment to the final

    result. Moreover, partnership enables GMP+ International to develop

    market oriented products and services.

    GMP+ International distinguish two types of partners: (i) trade associ-

    ations in the feed and animal production chain and (ii) food companies

    (dairy, eggs, meat, and aqua producers). Currently, GMP+ International

    has 35 partners (August 2015) representing the whole production

    chain of animal products. The 35 partners of GMP+ International are

     two international and 30 national trade-associations (in 7 different

    countries), as well as three international operating food companies,

    all together covering the whole feed and food chain. The goal is to

    increase partners’ participation in the relevant countries as much as

    possible.

    As mentioned before, we strive to a well-balanced participation of

     the different stakeholders in the whole chain. The aim is that there isnot any one single link in the chain that will dominate the decision-

    making and the division of the seats of the expert committees aims to

    realise well-balanced participation of all links.

    However, the principle and common target is to focus on the needs

    of the ultimate goal: to provide animal products to the consumers

    which are safe for their health and produced in a responsible way.

    Partnership means that the partner endorses this principle and it is

    a touchstone for the decision-making process. Besides that, we seek

     to reach consensus in decision-making as much as possible. Therefore

    multi-stakeholders’ dialogues contribute to reducing conflicts between

    interest groups.

    Indeed, partnerships limit participation to the stakeholders’ groups,

    which applied for partnership. Their representative experts are initially

    and finally involved in the decision making process.

    However, we apply also public consultation in order to enable

    everyone who is not involved via partnership, to give comments, to

    share expertise and to promote its interests. The results of the public

    consultation are considered seriously and taken into account in the final

    decision-making. We make also publish, when and why we do not take

    over comments, with an objective motivation.

    In multi-stakeholders’ dialogues, we realise that there are different

    interests: own interest, common interest (of industry) and public

    interest (of society). Our aim is to promote the common interest,

    which fits very well with the public interest of safe and response food

    of animal origin. Leading arguments are objective motivation, and anappeal on accountability and social responsibility of the feed business.

    Above the different interests, the dialogue is also influenced by the dif-

    ferences of cultural dimensions of the participants. That makes such

    dialogues not always easy, interesting and sometimes it cost time. The

    added value is worthwhile: it is reducing conflicts and contribute to

    better results.

     i  i  i   i  

      i     i    

     

    i      

    i        

    i           

    i                    

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    Get more daily industry news at the

    Aquaculturists www.theaquaculturists.blogspot.com

    Want more industry news?Try our daily online news service.

    Well-balanced multi-stakeholders’

    participation

    by Johan den Hartog, Managing Director, GMP+ International

    8 | INTERNATIONAL AQUAFEED | September-October 2015

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    Aller Aqua opens

    subsidiary in

    Nigeria

    Aller Aqua Nigeria is now

    a reality and the first con-

     tainer s with feed have

    arrived. A widespread net of local

    distributors are ready to sell Aller

    Aqua’s high quality fish feed in the

    African country.

    “It has not been easy getting

     this far, but Nigeria has so much

    potential – both in terms of edu-

    cating existing farmers with regard

     to maximising the yield of their

    production, but also in terms of

    expanding the aquaculture pro-

    duction in the country” explains

    Hans Erik Bylling, CEO of Aller

    Aqua Group. Niels Lundgaard,

    International Relationship Managerat Aller Aqua agrees and continues:

    “Nigeria is undergoing massive

    development at the moment,

    and implementing more aquacul-

     ture is a good and relatively cheap

    way of starting a business. We can

    help both established fish farmers

    and newly started ones by pro-

    viding extruded fish feed of a high

    quality, which can give the fish

    farms a boost in terms of produc-

     tion and economy. Further, we can

    help and guide them with the chal-

    lenges they may face in their pro-

    duction to the benefit of everyone

    involved.”

    Local partners, supported by Aller

    Aqua Nigeria, are ready to take

    good care of the customers. It is

    Aller Aqua’s philosophy that the cus-

     tomers must prosper in order for

     the company to do so, and thus, the

    Danish company have made every

    arrangement for the best possible

    start in Nigeria. Aller Aqua has

    moved fast, and offices and storage is

    ready, as well as 12 employees.

     Aller Aqua Nigeria will be par-

     ti cipa ting in three upcomingevents; “DELTA 2015” arranged

    by FISON (Fisheries Society of

    Nigeria), Agra Innovate Nigeria

    and CAFAN’s (Catfish Association

    of Nigeria) bi-annual fish festival –

    all in November.

    “This will be a great chance to

    meet our customers first hand,”

    explains Lasisi Nurudeen, Country

    Manager Nigeria. “In order to be

     taken ser iously we need to be

    seen in the right places, and par-

     ticipate actively where the fish

    farmers are. The two events will

    draw large crowds of both existing

    and potential fish farmers. We

    can help them set up and better

     their production. I have visited the

    Danish and German factories and

    seen the production, the quality

    control, the research station, Aller

    Aqua Research, and it is a great

    set up. The extruded feeds are

    very effective compared to what

    we are used to in Nigeria, and

    will better the productions here.

    It is a highly interesting product

    for the Nigerian market, and I am

    excited to be part of this projectfrom the very beginning.” Lasisi has

    a background in animal nutrition,

    and has studied Animal Production

    and Fisheries at the Lagos State

    Polytechnic, Lagos.

    Besides participation in local

    events and customer and partner

    visits, Aller Aqua Nigeria are

    planning on hosting seminars to

    further educate local fish farmers

    on the benefits of extruded

    fish feed and other subjects of

    interest. The company is also

    ready to collaborate with edu-

    cational institutions in terms of

    exchanging technical knowledge

    and research.

    Despite the fact that Aller Aqua

    Nigeria has only recently become

    a reality, the first feed has already

    arrived in the African country, and

    more is underway, underlining the

    great potential here. With stocks

    selling out quickly, it seems that

    one of the biggest challenges will

    be to ensure availability of the

    feeds at all times.

    The establishment of Aller Aqua

    Nigeria adds another country to thelist of Aller Aqua subsidiaries.

    Aller Aqua now exports fish

    feed to nearly 70 countries and

     the company supply feed for more

     than 30 species of fish and crus-

     taceans.

    Yara enters

    agreement to sell

    its European CO2 business

    Yara International ASA

    has signed a non-binding

    Heads of Terms with

    US-based Praxair, Inc to sell its

    European CO2 business for

    EU€218 million. The agreement

    also includes a sale of Yara’s

    remaining 34 percent stake in

     the Yara Praxair Holding AS joint

    venture to Praxair for an esti-mated EU€94 million.

    “The CO2 business has been an

    attractive and long-standing part

    of Yara’s portfolio, but remains a

    relatively small part of the broader

    industrial gas industry, and wherePraxair is well positioned to create

    additional value. I am confident

     that this business will be further

    strengthened under Praxair’s

    ownership, and at the same time

     this agreement allows Yara to

    redeploy management and finan-

    cial capacity to other value-cre-

    ating opportunities” said Svein

    Tore Holsether, president and

    Chief Executive Officer of Yara

    International ASA.

    The proposed transaction isconditional upon final transac-

     tion agreements, obtaining nec-

    essary approvals from com-

    petition authorities, and other

    customary closing conditions.

    The transaction is expected to close in the first quar ter of

    2016, with a provisionally esti-

    mated post-tax gain of EU€150

    million including the Yara Praxair

    Holding AS sale.

    In 2014, Yara’s European CO2 

    business sold more than 850

     thousand metr ic tons of liquid

    CO2 and 50 thousand metric tons

    of dry ice, delivering an EBITDA

    of EU€21.5 million and revenues

    of EU€112 million primarily from

     the food and beverage industry.The business operates five CO2 

    liquefaction plants, 3 CO2  ships,

    seven ship terminals and six dry

    ice production facilities.

    The Yara Praxair Holding

    AS joint venture, operating inScandinavia and formed in 2007,

    had a 2014 EBITDA of EU€35

    million and revenues of EU€145

    million (100 percent basis). Yara’s

    exit from the joint venture is

    regulated through a put/call

    option in the joint venture

    agreement.

    The Heads of Terms also

    includes an agreement for Yara to

    supply Praxair with raw CO2, gas

    and continue to operate three of

     the CO2 liquefaction units which

    are integrated within Yara’s ferti-

    liser plants.

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    Sachs Merchant

    Banking Division

    to acquire Hamlet

    Protein

    Altor Fund IV and Goldman

    Sachs Merchant Banking

    Divis ion partner to

    acquire the majority of Hamlet

    Protein from Polaris Private

    Equity and the founder of the

    company, Ole K Hansen.

    Hamlet Protein is a global

    provider of soy-based protein

    solutions used in high value-

    add animal feed for young

    animals. The company services

    more than 50 countries fromits two production facilities in

    Horsens, Denmark and Findlay,

    Ohio.

    “This is another important mile-

    stone for Hamlet Protein and

    we are very excited about our

    new partnership with Altor and

    Goldman Sachs Merchant Banking

    Division”, says Søren Munch, CEO

    of Hamlet Protein.

    “With the strong support

    of Altor and Goldman SachsMerchant Banking Division we

    are uniquely positioned to accel-

    erate the development and

    growth of Hamlet Protein and

    better service our customers and

    partners globally.”

      “We have made a successful

    management succession and

     turned Hamlet Protein into a

    leading global provider of specialty

    soya for young animal feed with a

    strong market position in Europe,US and Asia through developing

    global sales organization and sig-

    nificant investments in expanding

    capacity including establishing a

    sales and production facility in the

    US”, says Niels Worning, Partner

    at Polaris Pr ivate Equity.

    ”It has been a very successful

    partnership with Polaris, where

    we, along with a strong new lead-

    ership has managed to achieve

    a great development for Hamlet the recent year s with a signif-

    icant strengthening of Hamlet’s

    global market position,” says

    founder of Hamlet Protein, Ole

    K Hansen.

    “We are looking forward to

    continuing the positive develop-

    ment of Hamlet Protein started

    by Polaris in our equal part-

    nership with Goldman Sachs

    Merchant Banking Division”, says

    Søren Johansen, Partner at AltorEquity Partners. “We believe that

    Hamlet Protein is ideally posi-

     tioned to become the global

    champion within high value-add

    young animal feed.”

    “We are impressed by the

    high value-add young animal

    feed platform that the manage-

    ment team and employees have

    built under the current own-

    ership”, says Michael Specht

    Bruun, Managing Director in theMerchant Banking Division of

    Goldman Sachs.

    “We see significant growth

    potential globally and are excited

    about partnering with Altor and

     the management team to support

     the company’s impressive growth

     trajectory both organically and

     through acquisitions.”

    Closing of the transaction is

    subject to customary regulatory

    requirements and approvals. Wil liam Blair acted as exclu-

    sive financial advisor to the

    selling shareholders. Nordea has

    provided debt financing for the

     transaction.

    Cargill acquires EWOS

    for €1.35 billion

    Cargill has entered into

    an agreement with Altor

    Fund III and Bain Capital

    Europe III to acquire EWOS, a

    global leader in salmon nutrition

    for 1.35 billion euros. The trans-

    action, which is subject to reg-

    ulatory approvals, is expected

     to close before the end of the

    calendar year, says the company

    on its website today.

    “This transaction, which is sig-

    nificant and the second aqua-

    culture acquisition Cargill has

    announced in as many months, is

    a strategic investment in our long-

     term growth and evidence of

    our commitment to the growingaquaculture industry,” said David

    MacLennan, Cargill president and

    CEO.

    The acquisition gives Cargill

    entry into the salmon market and

    will make Cargill’s animal nutri-

     tion business a leading player in

     the growing salmon feed industry,

    one of the most advanced and

    professionally managed segments

    in global aquaculture. As part

    of the transaction, Cargill willacquire seven feed manufacturing

    facilities; three in Norway, and one

    each in Chile, Canada, Scotland

    and Vietnam, as well as two state-

    of-the-art R&D centres located

    in Norway and Chile. EWOS

    produces more than 1.2 million

    metric tons of salmon feed for

     the biggest salmon producers in

     the world.

    “EWOS is a winning company,”

    said Sarena Lin, president of

    Cargi l l ’s Feed & Nutrit ion

    business.

    “Adding its industry-leading

     talent and capabilities as well as

    its thought leadership in sustain-

    able business practices will be

     transformational for our aqua-

    culture nutrition business. We are

    looking forward to welcoming1,000 highly talented and pas-

    sionate EWOS employees to the

    Cargill Animal Nutrition team and

    strengthening our R&D capabili-

     ties and accelerating the pace of

    innovation to drive strategic long-

     term growth for Cargill’s animal

    nutrition business.”

    Einar Wathne, CEO of EWOS,

    said: “Cargill and EWOS will

    create a new, world-class aqua

    feed supply capability that will

    support the growth potential for

    fish and seafood consumption

    and create new opportunities for

    customers and employees alike.

    EWOS has a strong brand and

    a deep commitment to high

    product quality, its people and

    sustainable aquaculture. Adding

    Cargill ’s vast resources and

    global reach to EWOS’ leading

    R&D expertise, will allow the

    new organisation to provide

    world-class, innovative products

    and solutions to the global aquaindustry.”

    Cargill has established a strong

     track record of acquir ing and

    integrating businesses, unlocking

    additional value for customers by

    leveraging the talent and capabil-

    ities of acquired organisations in

    combination with Cargill’s global

    footprint, scale, breadth and

    depth of talent, and operational

    excellence, risk management, and

    expertise in animal nutrition, and

    supply chains.

    The acquisition adds to Cargill’s

    existing aquaculture capabilities in

    Mexico, Central America, China,

    United States, Southeast Asia, India,

    and Ecuador, to which EWOS is

    expected to contribute comple-

    mentary expertise and leadership.

     With this investment, the company

    will continue to leverage its global

    research and development capa-

    bilities, which includes 15 R&D

    and Technology Application facili-

     ties around the world.This acquisition is the second

    Cargill investment in aquacul-

     ture over the las t month. In

    early July, Cargill announced a

    US$30 million joint venture with

    Naturisa to build a shrimp feed

    facility in Ecuador.

    “With the need for protein

    expected to grow by 70 percent

    worldwide by 2050, farmed fish

    and shrimp offers one solution to

    meeting this demand, and Cargill

    intends to play a major role in this

    growing and important market,”

    Ms Lin said.

    David MacLennan, Cargill presidentand CEO EinarWathne, CEO of EWOS

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    Tour report Chile: Industry welcomes

    new Version five of the GLOBALGAP

    Aquaculture Standard

    K

    ey players in the

    Chilean aquacul-

    ture industry met

    on August 18,

    2015 in Puerto Varas at the

    GLOBALGAP Tour 2015 stop

    organised by DNV GL, one of

    the world’s leading certification

    bodies, for a presentation of

    the new Version five of the

    GLOBALGAP Aquaculture

    Standard and its impact on

    the aquaculture sector in the

    country. The new version,

    available since July 2015, incor-

    porates new elements that

    focus on a more sustainable andsocially responsible aquaculture.

    Latin AmericaReporting on all GLOBALGAP

    certification activities in Latin

    America on behalf of the

    GLOBALGAP Certification Body

    Committee, Jorge Ríos Alveal,

    Senior Consultant Food and

    Beverage at DNV said that more

     than 30 companies are certified

    under GLOBALGAP in Chile and

     that “the national salmon industry

    exports over 98 percent of its

    products.”

    Valeska Weymann, Senior

    Expert and Technical Key

    A c c o u n t G L O B A L G A P

    Aquaculture presented the

    new elements incorporated in

     the GLOBALGAP Aquaculture

    Standard Version five. Focused

    on Caring for Consumers

    and Responsible Sourcing at

    All Stages of Production, keychanges cover animal welfare,

    infrastructure specific to cage

    farming, area management,

    community , subcontract ing

    and workers’ health safety and

    welfare.

    Changes also include improve-

    ments to the overall language

    and structure of the document

     to ensure harmonised interpre-

     tation, with added detailed guide-

    lines to ensure animal welfare,

    as well as emphasising the sus-

     tainable aspects of the fish feed

    used.

    New requirements have been

    added to the criteria for the

    Veterinary Health Plan, which

    emphasise not only animal

    welfare but also the impact on

    both the environment and human

    beings, with regards to the use of

    antibiotics.

    International newsAs for recent international

    news on the high use of antibi-

    otics in Chile, Valeska Weymann

    explained that any GLOBALGAP

    certified company, including those

    in Chile, are only permitted touse antibiotics based on a diag-

    nosis.

    She added that, “we have

    been fortunate that the Rapid

    Alert System for Food and Feed

    (RASFF) used in Europe has

    not identified any weaknesses in

    GLOBALGAP certified products,

    which means that certification

    offers a high level of assurance

     that there are no residual chem-

    icals.”

    Version five of the Aquaculture

    Standard also enhances commu-

    nication with consumers.

    “Today, through the Friends of

     the Sea collaboration project, for

    example, we intend to promote

    higher communication addressing

    consumers’ key concerns such as

     the use of antibiotics,” explained

    Valeska Weymann.

    The head of the Department

    of the Environment Technological

    Institute of Salmon (INTESAL),

    Ximena Rojas, presented the

    macro-zone management pro-

    cesses taking place in the salmon

    farming and the current major

    improvements in health manage-

    ment in Chile, specifically since the crisis of the ISA (Infectious

    Salmon Anemia) virus in 2007.

    This crisis has accelerated the

    introduction of a new productive

    model for the Chilean aquacul-

     ture sector and strengthened the

    role of the national government

    in the control and regulation of

    aquaculture.

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    September-October 2015 | INTERNATIONAL AQUAFEED | 11

    FEATURE

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    Peas (Pisum sativum) are one of the

    four most important crops next to

    soybean, groundnut, and beans. It

    is a particularly important legume

    in temperate areas with numerous food

    (dry seed, vegetable) and feed (seed, fodder)

    usages.

    Field peas have attracted con-siderable attention as an animal

    feed due to increased produc-

     tion in Europe and Canada and

     thus reliable supply and com-

    petitive price. Peaseed meal has

    been trialed in Atlantic salmon,

    Rainbow trout and European sea

    bass with encouraging results.

    Peas, when unprocessed, con-

     tain moderate levels of protein

    22-25 percent but also several

    anti-nutritional factors (ANF);

    however, the levels of ANFs inpeas are low compared with

    other legumes and peas did not

    induce enteritis in the distal intes-

     tine in Atlantic salmon as seen

    when feeding soybean meal.

    New feed processing tech-

    nologies have provided more

    suitable products for use in high

    protein aquafeeds. Pea protein

    concentrate (PPC) is produced

    by fine grinding dehulled peas

    into pea flour, followed by airprocessing which separates the

    particles based on differences in

    size and density. The resulting

    concentrate has higher protein

    and lower carbohydrate and

    ANF contents compared with unprocessed

    peas, and thus would be a promising protein

    source in aquaculture feeds.

    The following study examined the efficacy

    and nutritional properties of peaseed protein

    concentrate as an alternative feed ingredi-

    ent and protein source for all-male tilapia

    (Oreochromis niloticus) culture incorporating

    growth and digestibility trials.

    Fish and rearing conditionsGenetically male Nile tilapia (GMT) were

    sourced as fry from Fishgen

    Ltd and raised at the Centre

    for Sustainable AquacultureResearch at Swansea

    University until they reached

     the appropriate sizes to be

    used in the trials. Fish were

    cultured indoors as part of a

    freshwater recirculation sys-

     tem, which included mechani-

    cal and bio-filtration units, a

    protein skimmer and a sand

    filter. Water temperature

    was kept at 27°C and pho-

     toperiod was set at 12 hours

    of light daily. Temperatureand dissolved oxygen levels

    were measured daily, while

     total ammonia nitrogen,

    nitrite, nitrate and pH were

    measured weekly to assure

    optimum water quality.

    Feed preparationFeeds were prepared

    according to Table 1 by mix-

    ing the dry ingredients with a

    binder and water, extruded through a meat grinder and

    afterwards dried at 450C for

    24 hrs. The resulting pellets

    had a diameter of 2.5mm and

    3.5mm for growth and digest-

    Pea Seed protein

    concentrate in

    Tilapia  feeds

    Table 1: Apparent digestibility coefcients (%) of sh meal and pea seed protein as

    determined in tilapia

    Dry matter

    %

    Protein

    %

    Organic Matter

    %

    Energy

    %

    Fish meal* 72.1 ± 0.2 89.5 ± 0.2 82.7 ± 0.3 83.7 ± 0.4

    Pea seed concentrate** 73.2 ± 0.1 88.4 ± 1.8 79.3 ± 2.2 80.3 ± 2.3

    Table 2: Formulation and composition of experimental feeds (per kg as fed)

    Ingredients Fishmeal 30% PPC 60% PPC 100% PPC

    Formulation

    Fish meal 605 410 210 -

    Pea seed concentrate - 165 340 520

    Corn Starch 325 340 320 310

    Vegetable Oil 40 45 50 55

    Vitamin &Mineral 5 5 5 5

    Di-calcium- Phosphate - - 25 55

    Calcium carbonate - - 25 55

    Alginate 25 25 25 25

    Composition

    Dry matter, g 927 905 903 910

    Ash, g 133 98.7 92.9 88.8

    Lipid, g 86.6 85.6 84.9 83.6

    Crude Protein, g 393 390 393 397

    Gross Energy, MJ 17.90 18.00 18.08 18.32

    Digestible Protein*, g 352 348 349 351

    Digestible Energy*, MJ 14.79 14.76 14.67 14.82

    DP/DE ratio g/MJ 23.8 23.5 23.8 23.7

    * Incorporating results from digestibility trial

    by Josh Cantril, Centre for SustainableAquaculture Research, SwanseaUniversity, Swansea, UK and IngridLupatsch, Aqua Nutrition Manager, ABAgri Ltd

    12 | INTERNATIONAL AQUAFEED | September-October 2015

    EATURE

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    ibility trial respectively and were stable up to

    24 hours in water.

    Sample preparation andchemical analyses

    At the start of the growth trial samples

    of about 10 fish were taken and at the end

    10 fish from each tank were sacrificed and

    blended to create homogenous samples. Sub-

    samples for estimation of dry matter were

     taken before the remaining homogenate was

    oven-dried. The dried samples were once

    more mixed in a blender before all remaininganalyses.

    Identical analyses were applied for diets,

    faecal matter and body homogenates. Crude

    protein was measured using the Kjeldahl tech-

    nique and multiplying N by 6.25. Ash was cal-

    culated from the weight loss after incineration

    of the samples for 12h at 5500C in a muffle

    furnace. Gross energy content was measured

    by combustion in a Parr bomb calorimeter

    using benzoic acid as the standard. Chromic

    oxide was estimated by wet digestion.

    Feed and faeces containing Cr2O3 were

    digested in a mixture of perchloric acid, con-

    centrated sulphuric acid and Na-molybdate.

    The resulting dichromate was determined at

    360 nm against Cr2O7 standard solutions.

    Digestibility trialDigestibility of the pea seed protein con-

    centrate was assessed by adding chromic

    oxide, an 0.8 percent inclusion, as the indi-

    gestible marker to the feed and collecting the

    faecal matter by siphoning. By assessing the

    ratio of marker to energy or nutrient in the

    feed compared to their ratio in faecal matter,

    digestibility of the nutrients can be established.

    Fishmeal was used as the reference ingredient

    and the test diet was mixed at a ratio of 50

    percent fishmeal and 50 percent pea seed

    protein. Fifteen tilapia, weighing on average

    400g, were stocked in 300L tanks to supply two replicates per treatment. Faecal matter

    from a given tank was pooled over the trial

    period until sufficient material was collected

    for subsequent analyses. Digestibility of ingre-

    dients were calculated using well established

    equations and are presented in Table 1.

    Growth trialFour experimental diets were formulated

    Table 3: Performance parameters of tilapia after 35 days of growth at 27oC (mean ± SD)

      Treatment Fishmeal 30% PPC 60% PPC 100% PPC

    Initial weight (g) 32.2 ± 1.0 32.5 ± 0.6 32.7 ± 1.2 33.4 ± 0.6

    Final weight (g) 97.8 ± 2.2 105.7 ± 1.0 100.9 ± 4.1 65.7 ± 3.2

    Weight gain g/sh/day 1.88 ± 0.03 2.09 ± 0.01 1.95 ± 0.08 0.92 ± 0.07

    Feed intake g/sh/day 2.06 ± 0.05 2.23 ± 0.12 2.09 ± 0.06 1.07 ± 0.02

    FCR 1.10 ± 0.05 1.06 ± 0.06 1.07 ± 0.02 1.16 ± 0.08

      Survival (%) 97 ± 4 100 ± 0 97 ± 4 82 ± 8

    Table 4: Composition of tilapia per g live weight (mean ± SD)

      Initial Fishmeal 30% PPC 60% PPC 100% PPC

    Dry Matter, mg 230 248 ± 0.7 274 ± 0.4 266 ± 1.2 247 ± 0.2

    Crude Protein, mg 127 139 ± 0.4 150 ± 4.0 149 ± 12.1 133 ± 2.1

    Lipid, mg 43.1 65.3 ± 4.0 80.9 ± 1.0 77.0 ± 1.0 63.7 ± 0.4

    Ash, mg 59.2 43.6 ± 2.4 43.2 ± 0.8 40.1 ± 1.1 50.4 ± 0.8

    Gross Energy, kJ 4.52 5.59 ± 0.18 6.63 ± 0.24 6.37 ± 0.37 5.29 ± 0.0

    September-October 2015 | INTERNATIONAL AQUAFEED | 13

    FEATURE

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     to contain 40 percent protein

    and nine percent lipid and

     to gradually include pea seed

    concentrate at the expense of

    fishmeal (Table 2). Seventeen

     tilapia of 35g initial size were

    stocked in 150L tanks provid-

    ing two replicates per treat-

    ment. The trial lasted 35 days

    and fish were fed manually

     to apparent satiation up to 4

     times daily. Any uneaten pel-

    lets were collected at the end

    of the day and accounted for.

    Thus feed intake was

    quantified and evaluated in

    relation to growth response.

    Through comparative body composition

    of fish carcass the relationship between

    dietary protein and energy intake and pro-

     tein and energy deposition was assessed,

    which allowed estimation of the utilisationefficiency of the feeds.

    Results and DiscussionComparison of growth performance

    among fish showed no difference when

    PPC supplied up to 60 percent of the

    protein in the diet. But growth obviously

    deteriorated in tilapia fed the 100 percent

    pea seed diet compared to the other three

    feeds. Survival was also slightly reduced

    (Table 3).

    Despite the poorer growth performance,

    results in Table 3 indicate that FCR was not

    different among the treatments (Table 3).Furthermore a trend can be inferred that fish

    performance improved with the inclusion

    of the pea seed concentrate. This differ-

    ence was not proven to be significant, but

    nevertheless higher weight gain, improved

    FCR (Table 3) and increased energy content

    (Table 4) can be described which ultimately

    resulted in improved energy and protein

    retention efficiencies

    (Table 5).

    The reduced weight

    gain was thought to be

    correlated with reduced

    feed intake in fish fed the

    100 percent pea seed

    feed as indicated in Table

    3.

    ConclusionsTilapia performed on

    feeds with pea seed pro-

     tein of up to 35 percent

    dietary inclusion very

    well. At this level - equiv-

    alent to 65 percent of

    fishmeal protein replacement - performance

    of tilapia was equivalent to fish when fed

     the all fishmeal feed.

    The reduced growth of tilapia fed the 100

    percent pea seed feed was mainly due todecreased feed intake and palatability might

    have been an issue. No significant differences

    were found with regards to FCR, energy and

    protein retention efficiency values. This fact

    supports the notion that whichever pro-

     tein source the fish consumed they utilised

    equally well.

    References available on request 

    Table 5 : Energy and protein retention efciency (mean ± SD) in tilapia fed pea seed meal at

    increasing inclusion levels.

      Fishmeal 30% PPC 60% PPC 100% PPC

    Digestible Energy intake

    (kJ / sh /day)

    30.5 ± 0.8 32.9 ± 1.7 30.7 ± 0.9 15.9 ± 0.2

    Energy gained

    (kJ / sh /day)

    11.5 ± 0.3 15.8 ± 0.6 14.1 ± 1.7 5.6 ± 0.3

    Digestible Energy

    retention efciency (%)

    37.7 ± 0.0 47.2 ± 0.7 46.0 ± 4.2 35.4 ±1.5

    Digestible Protein intake

    (g / sh /day)

    0.73 ± 0.02 0.77 ± 0.04 0.73 ± 0.02 0.38 ± 0.01

    Protein gained

    (g / sh /day)

    0.27 ± 0.00 0.34 ± 0.01 0.31 ± 0.05 0.13 ±0.01

    Digestible Protein

    retention efciency (%)

    37.5 ± 1.5 43.4 ± 1.0 42.7 ± 5.4 34.4 ±1.0

    14 | INTERNATIONAL AQUAFEED | September-October 2015

    EATURE

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    C

    atfish, which is a significant group of the fishes in

    wetlands, are economically important with a high nutrient

    value. Catfishes, owing to their unique taste, few bones,

    are considered a delicacy for the fish consumers, but

    production of different indigenous catfishes through aquaculture isunexplored in India, although aquaculture contribution of some of

    the catfish varieties like Ictalurus, Silurus and Clarias spp. has been

    exemplary in the World scenario.

    Catfishes are the second major group of freshwater fishes. India,

    being a mega-diverse country, harbors 197 catfish species from 52

    genera. The Indian families include Amblycipitidae (Torrent catfishes),

    Akysidae (Stream catfishes), Bagridae (Bagrid catfishes), Chacidae

    (Square head or angler catfish), Clariidae (Air breathing catfishes),

    Heteropneustidae (Air-sac catfishes), Olyridae (Long-tail catfishes),

    Pangasiidae (Shark catfishes), Plotosidae (Eel-tail catfishes), Schilbeidae

    (Schilbid catfishes), Siluridae (Sheat fishes), Sisoridae (Sisorid catfishes).

    Present statusConsidering the high market demand for catfish and the avail-

    ability of a huge potential resource in the form of many inland water

    resources, commercial farming of these species is presently receiving

    important attention. In India catfish production is coming from both the

    systems of inland resources i.e. capture and aquaculture.

    Capture: The inland water resources of the country are in terms

    of rivers, estuaries, backwaters and lagoons, reservoirs, floodplain

    wetlands and upland lakes, which contribute about 1.05 million

     tonnes of fish annually. The principal rivers of India as Yamuna, Ganga,

    Brahmaputra, Mahanandi, Godavari, Krishna and Cauvery including

     their main tributaries and distributaries harbors about 11.5 percentof the fish fauna so far know in the world. Many of the catfish species

    start from 15 grams size (Mystus tengara) to very large about 50 Kg size

    (Hemibagrus maydelli) harvested from these water sources in India.

    And many of the well-known food catfishes are also fine sporting fishes.

    Aquaculture: Ponds and tanks are the prime resources for freshwa-

     ter aquaculture; however, only about 40 percent of the available area

    is used for aquaculture currently. In northern India, open waters with

    in-flows are common, while southern India has watersheds, termed as

     tanks, largely used for crop irrigation along with carp fish culture.

    After carps, cat-fish production is also

    increasing tremen-

    dously throughout the

    India since 2001 (Table:

    1). Out of 197 catfish

    species available in

    Indian region, about 30

    catfish species are eco-

    nomical and which are

    produced mainly from

    capture fishery, and a

    couple of catfish spe-

    cies are produced fromboth the aquaculture

    and capture fishery in

     the country (Table: 2).

    There are six catfish

    Table 1: Catsh production in India from

    2001 to 2010

    Year Inland sh

    production

    (in tonnes)

    Catsh

    production

    (in tonnes)

    Catsh

    %

    2001 3084247 78367 2.54

    2002 3165927 101488 3.20

    2003 3220407 106282 3.30

    2004 3527505 78990 2.23

    2005 3727375 132342 3.55

    2006 3750736 130027 3.46

    2007 4170560 125800 3.01

    2008 3799563 174503 4.59

    2009 4660463 179843 3.85

    2010 5077200 212462 4.18

    Table 3: Commercially cultured Catsh species in India.

    Sl.

    No.

    Family Common Name Scientic Name

    1 Clariidae Magur Clarias batrachus (Linnaeus)

    2 Clariidae African catsh

    or Thai magur

    Clarias gariepinus (Burchell)

    3 Heteropneustidae Singhi or stinging catsh  Heteropneustes fossilis

    4 Siluridae Butter catsh Ompok bimaculatus

    5 Pangasiidae Pangas Pangasius pangasius

    6 Pangasiidae Sutchi catsh Pangasianodon

    hypophthalmus

     Catfish production in India: P r e  s e n  t   s  t a  t  u  s  a n  d     p r o  s   p e c  t  s by Dr B. Laxmappa, Fisheries Development Officer, Department of Fisheries,

    16 | INTERNATIONAL AQUAFEED | September-October 2015

    EATURE

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    species are cultured in India (Table 3). Among six, only two catfish

    species viz. Pangasianodon and African catfish culture is intensified in

     the country due to its higher production rates by using various local

    as well as commercial feeding methods. Of late, Government of India

    permitted culture of Pangasianodon and Tilapia species laying down

    strict guidelines as an alternative crop to carp fishes.

    Pangasius sutchi or Pangasianodon hypophthalmus one of the swift

    growing catfishes was first introduced into India in the year 1995–1996

    in the state of West Bengal from Thailand through Bangladesh. Initially

    farming was carried out in limited area in the States of West

    Bengal and Andhra Pradesh. This fish grows to 1–1.5 kg dur-

    ing one year. A minimum of 10–15 tonnes/hectare/year is

    harvested through due to culture of this fish.

    In commercial culture of Pangasianodon farmers are using

    both pelleted and extruded feeds. Due to high feed cost and

    fluctuation in production costs, in some areas farmers are usinglocal feeds like cooked corn grains, broken rice, chicken waste

    etc.

    African catfish or Thai magur, Clarias gariepinus culture was banned

    in India under Environment Protection Act. But many fishermen still

    cultivating this banned catfish illegally in village ponds to make a quick

    buck. This species was clandestinely introduced into the state of West

    Bengal possibly during 1994 from neighboring Bangladesh and quickly

    spread throughout the country, including into cold regions as well as

    coastal areas. It is largely cultured in the states of West Bengal, Punjab,

    Tamil Nadu, Karnataka, Assam, Maharashtra, Andhra Pradesh and

    Telangana. Clarias gariepinus is usually fed waste intestines and skin of

    chickens so it grows fast than compared toother local species catfish or carp’s fish with

    low feeding cost.

    ProspectsAquaculture in India has become an indus-

     try since late eighties with several entre-

    preneurs taking up aquaculture with carps,

    catfishes and prawns. Of late, Government

    of India has also identified catfish farming as

    a National Priority and has emphasised on

    diversification of culture practices. The major

    chunk of catfish, however, comes from cap-

     ture resources, which includes air breathingas well as non-air-breathing varieties. Air-

    breathing catfishes have greater potentiality to

    utilise shallow, swampy, marshy and derelict

    water-bodies for aquaculture; whereas non-

    air-breathing catfishes can be well suited to

    normal pond environment.

    Among the catfishes magur, Clarias batra-

    chus is the only species that has received

    much attention. Singhi, Heteropneustes fos-

    silis is another air-breathing catfish species

    being cultured to a certain extent in swamps

    and derelict water bodies, especially in theeastern states. In recent years, attempts have

    been made to develop the culture of non-air

    breathing catfishes like Pangasius pangasius,

     Wallagoattu, Aorichthys seenghala, A. aor and

    Ompok pabda.

    Table 2: Important commercial Catsh species and source of production in India

    Sl.

    No.

    Species Family Source of

    production

    Common name Scientic name

    1 Gangetic mystus  Mystus cavasius (Hamilton) Bagridae Capture

    2 Day’s mystus  Mystus bleekeri (Day) Bagridae Capture

    3 Tengara mystus  Mystus tengara (Hamilton) Bagridae Capture

    4 Gogra rita  Rita gogra (Sykes) Bagridae Capture

    5 Giant river catsh  Aorichthys seenghala (Sykes) Bagridae Capture

    6 Long-whiskerd catsh  Aorichthys aor (Hamilton) Bagridae Capture

    7 Krishna mystus  Hemibagrus maydelli (Rossell) Bagridae Capture

    8 Indian butter-catsh Ompok bimaculatus (Bloch) Siluridae Capture

    9 Pabdah catsh Ompok pabda (Hamilton) Siluridae Capture & Aquaculture

    10 Boal Wallago attu (Bloch & Schneider) Siluridae Capture

    11 White catsh Silonia children (Sykes) Schilbidae Capture

    12 Khavalchor catsh  Neotropius khavalchor Kulkarni Schilbidae Capture

    13 Goongwaree vacha  Eutropiichthys goongwaree

    (Sykes)

    Schilbidae Capture

    14 Indian potasi Pseudeutropius atherinoides

    (Bloch)

    Schilbeidae Capture

    15 Gangetic goonch  Bagarius bagarius (Hamilton) Sisoridae Capture

    16 Stinging catsh  Heteropneustes fossilis (Bloch) Heteropneustidae Capture & Aquaculture

    17 Magur Clarias batrachus (Linnaeus) Clariidae Capture & Aquaculture

    18 African catsh Clarias gariepinus (Burchell)* Clariidae Aquaculture

    19 Pungas Pangasius pangasius (Hamilton) Pangasidae Capture

    20 Sutchi catsh Pangasianodon hypophthalmus* Pangasidae Aquaculture

    *Exotic species

    September-October 2015 | INTERNATIONAL AQUAFEED | 17

    FEATURE

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    Following the successful launch of

    its disease diagnostics laboratory

    facilities in Bangkok, Thailand in

     July 2014, Fish Vet Group Asia

    Ltd has now opened wet laboratory and

    challenge testing facilities in Chonburi, 90

    minutes south-east of Bangkok.

    The facility can accommodate confiden-

     tial, replicated growth trials designed to clientspecific protocols for freshwater, brackish

    water and marine shrimp and fish spe-

    cies and both bacterial, for example Vibrio

    parahaemolyticus, and viral, including WSSV

    challenge tests.

    Best bio-security practicesThe unit, under the watchful eye of

    aquatic veterinarian Dr Leo Galli, is operated

    under best biosecurity practices. All incoming

    stock are screened at source and regardless

    of any accompanying specific pathogen free(SPF) status certification are only allowed

    into the facility after reconfirmation of their

    SPF status by conducting tests in the FVG

    Asia Ltd disease diagnostics laboratories in

    Bangkok.

    Additional mandatory practices on site,

    include disinfection of incoming water, pipe-

    work and facility equipment.

    The facilitiesA series of 400-litre external nursery

     tanks are used to grow seed (shrimp PL’s or

    fish fry) to the preferr