Tissues and OrgansComprising the Immune

Response System

Where components of the immune responseoriginate,

differentiate, meet,

interact, and function

Folder Title: Tiss&OrgNoTPUpdated: November 10, 2015

APC and T-Cells

Marcophage and T-Cell Kissing

Macrophage to T-Cell Interaction

Helper T-cell B-Cell Interaction

CD4 co-receptor

B-Cell Activation

See figure 2-7,Kuby, 4th Ed.,p. 36

B-Cell: Small, Blast, and Plasma

See Figure 2-7,Kuby 4th Ed.p. 36

Where do these cells originate?

Where do they go to interact with each other?

Where do they carry out their functions?

Primary and Secondary Lymphoid

Related to Figure 2-13, Kuby, 4th Edition, p. 47

AnimationsProduced for Kuby Immunology

Online Link

http://bcs.whfreeman.com/immunology6e/

(In Class Do animations from the Book CD)

Chapter 2 Animation. Cells and Organs

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Blue thing

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1 2 3 4 5 6Purple Thing

Thymus Photo

Thymus Cartoon

Diagrammatic Cross Section of Thymus. Figure 2-14, Kuby, 4th Edition, p. 48 ThymusCross

CD4 and CD8 in Thymus

MALT and M-CellsSee Figure 2-22, Kuby 4th Ed.

p. 56

MALT and IgA

See Figure 2-22Kuby, 4th Ed.p. 56

CD Antigen Table

CDMarks

CD Antigens Color Coded

AnimationsProduced for Kuby Immunology

http://bcs.whfreeman.com/immunology6e/

Chapter 11 Animation. Signal Transduction

CD Antigen List: Pages A1 to A26: 339 Entries, 2007 Edition of Kuby

How to Understand CD Antigens: 5 Persons at a University

Person 1: 5 ft 5”, 160 lbs bespectacled old geezerPerson 2: 5 ft 11”, 280 lbs, 22-year old guy, all musclePerson 3: 5 ft 5”, 135 lbs, 20-year-old womanPerson 4: 7 ft 1” , 200 lbs, 22 year-old guyPerson 5: 5 ft 8”, 150 lbs, 22 year-old guy

Which Person:A. Is a varsity basketball player?

B. Plays on the women’s field-hockey team?C. Is a boring old professor?

D. Is a varsity football player for SU?E. Is a men’s cross-country runner?

Person 1: 5 ft 5”, 160 lbs bespectacled old geezerPerson 2: 5 ft 11”, 280 lbs, 22-year old guy, all musclePerson 3: 5 ft 5”, 135 lbs, 20-year-old womanPerson 4: 7 ft 1” , 200 lbs, 22 year-old guyPerson 5: 5 ft 8”, 150 lbs, 22 year-old guy

1C; 2D; 3B; 4A; 5E

Signal, Receptors, and co-receptor Pairs

Secrets of Signaling1. How Do You Knock on a Door? Why that way?

2. Why does the phone “ring” more than once?

3. Why can the police officer directing traffic blow his whistle only once?

4. Why does your computer say “Are You Sure You Want to Delete File XYZ?”

5. When the class was directed to do “Bunny Ears” behind the person next to them, only a few persons responded. Why was that?

6. A different set of persons responded when asked to raise their right hand. Why was that?

7. No one responded when the class was asked to stand up. Why?

Secrets of Signaling (2)

8. Only the guys in the front of the room responded when I invited the class to do what all classes would love to do to a pain-in-the neck Prof. Why was that?

9. I asked the class in plain English to raise both hands but no one complied. Why is that? What did that illustrate about properties of signaling.

10. I gave the answer to the coming quiz question to the entire class in plain English but no one heard the answer even though I said it clearly and loudly. Why not? What did that illustrate about cell signaling?

11. Why does the T-Helper Cell physically attach itself to the macrophage? What is the advantage of that?

Costimulatory signals: B7 – CD28

To Here: Nov. 4, 2014; 2nd Exam Nov. 6th

Costimulatory Signals: T-Cell to MPh

Cancer Eluding Costimulatory Signal

CD’s on B, T, and NK Cells

Surface Determinants on T-Lymphocytes:Note: CD4 THCD8 TCCD28 and CD3 Both.NK Determinants:CD56CD16CD8

Surface Determinants on B-Cells:ImmunoglobulinsB7

CD Markers in Leukemia

DiagnosisStagingPrognosisTreatment

T-Cell ALL Markers

Kuby, 5th Editionp. 157

(“ALL” = Acute Lymphocytic Leukemia)

Pre-B-Cell ALL Markers

Kuby, 5th Editionp. 157

(“ALL” = Acute Lymphocytic Leukemia)

B-Cell CLL Markers

Kuby, 5th Editionp. 157

(“CLL” = Chronic Lymphocytic Leukemia)

Leukocyte Recirculation

How Do Circulating Hematopoietic Cells “Know” Where They Are?

How Do they Stick?

How Do they Move?

Lymphocyte Recirculation

Leucocytes in Capillaries Cartoon

Leucocytes in Capillaries Photo

Extravasation (Getting out of the Blood Stream)of Naïve T-Cell into Lymph Node

Adhesion: Leucocytes to Endothelium

CAM =Cell Adhesion Molecule

(Non-inflamed Endothelium)

CAM = Cell Adhesion Molecules

General Structures ofCell Adhesion Molecules (CAMs) Kuby, 3rd Edition Figure 15-2a

Endothelial Cells

Lining Capillaries

CAMStruct

Membrane of CirculatingLeucocytes

CAMinCap Adhesion in Capilaries

(Inflamed Endothelium)

Neutrophils trails guide virus-specific CD8+ T cell migration.

Kihong Lim et al. Science 2015;349:aaa4352

Published by AAAS

Chemokine from Uropod of Neutrophils

Neutrophils trails guide virus-specific CD8+ T cell migration.(CXCL12 is a chemokine: chemical agent attracting cell movement)

Kihong Lim et al. Science 2015;349:aaa4352

Published by AAAS

Virus-specific Cytotoxic T-Cell

Blebs containing CXCL12 from Uropod (Trailing membrane region) of Neutrophil

Cytotoxic T-Cell migration through trachea to virus-infected cells

“Bread-crumb” trail

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Slides Beyond this Point Not included in BIO447, Fall, 2014

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5. This isn’t pitched at a level appropriate for an upper division undergraduate course. Please move to a higher level of challenge.

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AnimationsProduced for Kuby Immunology

http://bcs.whfreeman.com/immunology6e/

Or search “Kuby Immunology”, Click on “Kuby Immunology 6e” , go to Student Resources

Chapter 2: Cells Cell Death (Also chapter 10)

Chapter 11: Signal TransductionChapter 13: Leucocyte Extravasation

Actin Cytoskeleton Linkage to Collagen Extra-cellular Matrix

Figure 19-14 ECB

1998, p. 603

Plasma Membrane

Collagen

Integrin

Fibronectin

Actin Microfilament

MFtoECM

CAMFamlyOn EndothelialCells

On Neutrophils

On Mucosal Epithelium

On Leucocytes

On EndothelialCells

On Leucocytes

On EndothelialCells

Families ofCell AdhesionMoleculesKuby, 3rd Ed.Figure 15-2bCamFamly

Leucocyte CAM in BlueTissue-target Cells in Red

AnimationsProduced for Kuby Immunology

OnLine Linkhttp://bcs.whfreeman.com/immunology6e/

In Class Use Book CD:

Chapter 2: Cells of the Immune-system (Static)Cell Death : (Animated)

Chapter 11: Signal Transduction (Animated)Chapter 4 : Immunoglobulins (Static)

Chapter 13 Animation. Leukocyte Extravasation (Animated)

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