march | april 10 - international aquafeed

50
THE INTERNATIONAL MAGAZINE FOR THE AQUACULTURE FEED INDUSTRY Member of the World Aquaculture Society, European Aquaculture Society, American Feed Industry Association and the International Aquafeed Association VOLUME 13 ISSUE 2 2010 Protein sources - Functionality the key to a role in modern aquafeeds Extrusion: - Protein damage/complexation during extrusion Evaluation of probiotic bacteria in prawn production

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International Aquafeed Volume 13 Issue 2, Featuring: Protein sources - Alternative protein sources should also show functionality if they are to play a role in modern aquafeeds • Evaluation of probiotic bacteria in prawn production • Protein damage/complexation during extrusion • A vacuum batch coating system especially designed for aqua feed and pet food • Aquafeeds - ready to take the plunge • Feed Management - Aquafeed in Tanzania • Feed Management - Diets for tilapia brood stock: Addressing challenges for reducing dependency on fish oil

TRANSCRIPT

Page 1: March | April 10 - International Aquafeed

the international magazine for the aquaculture feed industry

Member of the World Aquaculture Society European Aquaculture Society American Feed Industry Association and the International Aquafeed Association

Volume 13 I s sue 2 2 010

Protein sources - Functionality the key to a role in

modern aquafeeds

Extrusion - Protein damagecomplexation

during extrusion

Evaluation of probiotic bacteria in prawn

production

Volume 13 Issue 2 March-April 2010 copy Copyright Perendale Publishers Ltd 2010 All rights reserved

Aquaculture UK 2010 19-20 May 2010 at Aviemore

Now an established major event Aquaculture UK will be held again in May 2010With visitors from 18 countries as far afield as Sweden Chile and the United States

make sure you donrsquot miss out on this opportunity to connect with buyers and decision makers from the aquaculture industry worldwide

Herersquos what exhibitors said of the 2008 exhibition

ldquoAviemore was a good location and the show had a certain spirit which is always hard to createrdquo Guy Mace - MD Biomar

ldquoThe show went well for EWOS - our costs were much lower than Glasgow and we got the audience we wantedrdquo Douglas Low - MD Ewos

ldquoThe feedback regarding Aviemore seems to have been very positive and it had a much better atmosphere than GlasgowrdquoWill Jewison - Skretting

ldquoMany thanks for delivering a very successful exhibition and return to Aviemorerdquo Alan Stewart - Director Landcatch

aquaI n t E r n A t I o n A l

feedAn internAtionAl mAgAzine for the AquAculture feed industryCONTENTS

PerendalePublishers Ltd International Aquafeed is published six times a year by Perendale Publishers Ltd of the United Kingdom

All data is published in good faith based on information received and while every care is taken to prevent inaccuracies the publishers accept no liability for any errors or omissions or for the consequences of action taken on the basis of information published copyCopyright 2010 Perendale Publishers Ltd All rights reserved No part of this publication may be reproduced in any form or by any means without prior permission of the copyright owner Printed by Perendale Publishers Ltd ISSN 1464-0058

Volume 13 Issue 2 March-April 2010 copy Copyright Perendale Publishers Ltd 2010 All rights reserved

EDITORrsquoS DESK 2

Aqua NewsA bold step for BIOMIN aquaculture 3Strategic Cooperation Agreement between CPF and AKVA 4Perten Instruments appoints managing director in Australia 4ILDEX Vietnam 2010 the solutions for Vietnam sustainable growth 5The future of 6marine aquaculture 6NOREL announces new web site and new corporate image 8Kiotechagil to show pHorceful presence at VIV 8ARCHIVE 8Dishman appoints Chris Oates as chief operating officer 9New role will oversee Dishman Indian and Chinese Facilities 9First certification awarded under the IFFO Global Standard for Responsible Supply 10Industry support strengthens for 2010 OSM Conference 11

ProteinProtein sources - Alternative protein sources should also show 12 functionality if they are to play a role in modern aquafeeds

ProbioticEvaluation of probiotic bacteria in prawn production 18

THE AQUAFEED PHOTOSHOOT 24

ExtrusionProtein damagecomplexation during extrusion 26

VacuumA vacuum batch coating system especially designed for aquafeed and pet food 32

AquafeedsAquafeeds ready to take the plunge 34

Feed ManagementAquafeeds in Tanzania 36Diets for tilapia brood stock Addressing challenges for reducing dependency on fish oil 38

Book Reviews 42

CLASSIFIED ADVERTS 44

IAF WEB LINKS 46

AQUA EVENTS 48

Cov

er im

age

cour

tesy

of

Fusi

on M

arin

e Li

mite

d

EDITORrsquoS DESK W

ELC

OM

E TO

INTE

RN

ATIO

NA

L AQ

UA

FEED

MA

GA

ZIN

E

Croeso (Welcome)

As we leave a very cold dark winter behind us in Northern Europe and the USA we look towards the glimmer of spring and the start a new produc-tion cycle in the Northern Hemisphere Of course this contrasts with the tropics where demand for fish and shellfish production is expanding and

the aquafeed sector must respond accordingly

SE Asia continues to dominate and it is not surprising that International Aquafeed continues to receive invaluable contributions from specialists in this region

I am delighted to overview the contribution of Keith Filer from Alltech Asia ndashPacific concerning the development of novel proteins based on single cell sources such as yeasts and algal blends and how this might contribute significantly to diet formulations for many fish species in future

He focuses on some recent scientific appraisals of products from both a production basis and promotion of health

This fits in with the overall global sustainability agenda and is further elaborated by Daphne Tanrsquos feature which takes a more holistic view of the protein and feed ingredient needs in aquaculture and addresses this topic in an interesting approach from Singapore with an expert eye on the market trends and potential for raw material usage

My editorial associate Professor Krishen Rana from Stirling again addresses feed management issues with his sound knowledge of Africa and this time turns his attention to Tanzania and tilapia and in particular follows with a specialised focus on tilapia brood stock diets

Indeed we must in future include a special feature on the nutritional requirements of brood stock fish in general as the aquafeed industry always needs to produce a range of special feeds for shrimp maturation and large mature fin-fish entering their reproductive stages

As such feed management is an important aspect of good husbandry and I thank Krishen for his excellent contributions to this section and wide applications to effective feed delivery and efficiency gains

The technical issues relating to feed manufacture are highlighted in an article concerning protein damage and stability in the complex extrusion process This is a very important concern and is per-tinent to our understanding of the delicate nature of many feed ingredient and how their nutritional value can be modulated under various conditions We also note the importance of post-extrusion vacuum coating technology for specialised feed additives and oil inclusion

Finally I mention a feature from one of our youngest contributors Tim OrsquoBrine who at 22 is one of my MSc students undertaking his Masters in lsquoSustainable Aquaculture Systemsrsquo

His article reviewing the use of probiotics in prawn production is comprehensive and a useful staging post for further consideration on disease and health management of shrimp

I am sure you will enjoy this spring issue next time I will report directly from the World Aquaculture Society meeting in San Diego and which will no doubt provide many new topics for discussion

March-April 2010

SubSCribEwwwaquafeedcouksubscribephp

International Aquafeed is published six times a year bringing you in-depth features industry news events book reviews and more Subscribers to International Aquafeed also receive a free copy of the International Aquafeed Directory worth UKpound85 For more information please visit our website

2 | InternatIonal AquAFeed | March-april 2010

EditorProfessor Simon DaviesTel +44 1242 267706Email simondaquafeedcouk

Associate EditorProfessor Krishen RanaTel +44 1242 267706Email krishenraquafeedcouk

Editorial Advisory Panelbull Abdel-Fattah M El-Sayed (Egypt)bull Aliro Borquez (Chile)bull Chen Shuping (China)bull Citas Pascual (Philippines)bull Colin Mair (UK)bull Daniel Montero (Spain)bull Dom Bureau (Canada)bull Eric De Muylder (Belgium)bull Miguel A Olvera (Mexico)bull Mike Cremer (USA)bull Ola Flesland (Norway)bull Mohammad R Hasan (Italy)bull Ming DanChen (Thailand)

International Marketing Manager

Caroline WearnTel +44 1242 267706Email carolinewaquafeedcouk

Subscription amp Circulation Manager

Tuti TanTel +44 1242 267706 Email tutitaquafeedcouk

Production Manager

Nicky BarnesTel +44 1242 267706 Email nickybaquafeedcouk

Design amp Page Layout

James TaylorTel +44 1242 267706 Email jamestaquafeedcouk

2 | InternatIonal AquAFeed | March-april 2010

Tel +1 314 739 9191 bull +1 800 288 2726 bull Fax +1 314 739 5880 bull wwwtapcoinccomSt Louis Missouri USA

ELEVATOR BUCKETS - ELEVATOR BOLTS

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(Speaks English)

ldquoRounded edges =

gentle handlingrdquo

Wherever your product is processed storedor shipped one thing is constantthe

need for high quality buckets thatstand the test of time

Since 1974 Tapco buckets havebeen outperforming all othersin tests and actual usage ndashhandling everything from

feed to fertilizer with care andefficiency Maybe thatrsquos why

Tapco has the two most popular designs in the world Tapco hasa bucket that will interchange and replace existing buckets to giveyou the durability you need to handle abrasive loadshellipwith equal or greater capacity

When you call Tapco yoursquoll be pleased to learnthat your representative literally DOES speakyour language ndash and knows which bucketsare the best choice for your application Visitwwwtapcoinccom to find out moreabout Tapcorsquos world-class features

Thorsten Rogner Europe amp Asia(Speaks German)ldquoCC-XD ndash Toughest on the marketrdquo

Bruce Fagala USA (Speaks English)

ldquoNon-sparking and saferdquo

Jimi Davidson USA (Speaks Spanish)ldquoAvailable in polyethylenepolyurethane and nylonrdquo

FANGED HEADElevator Bolt

FLAT COUNTERSUNKHEAD (NO 1 NORWAY)

Elevator Bolt

Carl Swisher USA amp Canada

(Speaks Spanish)

ldquoXtremely efficientrdquo

Yassine Abbad Middle East amp Africa(Speaks French amp Arabic)ldquoBuckets for every applicationrdquo

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Mike Kerlagon USA (Speaks English)

ldquoSame day shipping availablerdquo

When it Comes to Elevator Bucket PerformanceTapco Speaks Your Language

reg

STYLE SUPER EUROBUCKETtrade CC-XD(XTREME DUTY) amp CC-HD (HEAVY DUTY)

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The color blue when used in connection with elevator buckets is a US registered trademark owned by Tapco Inc Super EuroBucket trade is a registered trademark of Tapco Inc copy 2010 Tapco Increg All rights reserved

SpeakAd_FINALA4 22310 102 AM Page 1

Strategic Cooperation Agreement between CPF and AKVA

Charoen Phokphand Foods Ltd the worlds leading producer and

suppl ier of farmed White Shrimp and AKVA group ASA the worlds leading supplier of technology for the aquacul-ture industry have signed a stra-tegic cooperation agreement for developing a complete new production system for the pro-duction of white shrimp utilising AKVAs recirculation technology and CPFs broad knowledge in shrimp farming

CPF is working actively to improve the i r product ion methods and technology

A natural next step for the shrimp farming industry is to utilise new technological oppor-tunities to address many of the challenges of the industry and society today

ldquoThis strategic cooperation agreement with AKVA is an important step for us in our strive

towards a transformation of the shrimp farming industry

ldquoBy th i s pro ject we are addressing impor tant issues for the industr y such as the level of which marine resource and land areas are utilised the escaping of shrimp from farms to interfere with wild stocks the control of diseases and efficient battling of parasites the elimi-nation of and risks of pollution from shrimp farms and other possible effects on the habitats where the farming is done Further to this we believe that the technology that this stra-tegic cooperation agreement will produce has the potential also to limit the carbon foot-print of the produced shrimp significantlyrdquo says Dr Chingchai Lohanawatanakul Chairman of the executive Board of CPF

The first step of the strategic cooperation includes developing a full scale prototype of a 200

tonnesyear intensive facility for white shrimp (Litopenaeus Vannamei) as an integrated solution

ldquoFor AKVA group this is an important step forward in our development in the Asian market and the furthering of our recir-culation technology in the global market

ldquoWe appreciate the initia-tives and eagerness that CPF is showing in this project and are looking forward to the results from this cooperation for both par tiesrdquo says Mr Knut Molaug CEO of AKVA group

Mo re in fo rMatio n

Knut MolaugCEOAKVA group ASA Nordlysveien 4PO Box 271 N-4349 BryneNorwayTel +47 901 39522Fax +47 51 778501Email kmolaugakvagroupcom

Back from left Mr Robins P McIntosh (Senior VP CPF) Mr Jan Erik Svensson (Regional Director AKVA group S E Asia) Dr Chingchai Lohawatanakul (Chairman of the Executive Board CPF)Front from left Mr Knut Molaug (CEO AKVA group) Dr Sujint Thammasart (Executive VP CPF)

Je f f R o g e r s h a s b e e n appointed managing director to lead Per ten Instruments

of Australia Pty Ltd He brings over 25 years expe-

rience in the scientific instrument equipment marketplace and is well qualified to lead the company in delivering our world class tech-nology and support

ldquoThis is a very exciting time for

our company We are all looking forward to a great 2010 with our customers and suppliersrdquo says Mr Rogers

He takes over from Rodney B o o t h w h o h a s r e t i r e d a s manag ing d i r ec to r and w i l l now t a ke a Bu s i ne s s Development role within the l a r ge r Per ten In s t r ument s G r o u p R o d n e y f o u n d e d N e w p o r t S c i e n t i f i c a n d l aunched the Rap id V i s co Analyser (RVA) in 1985

ldquoI am looking forward to my new focus on strategic projects a n d r e s e a r c h a n d d e ve l o p -ment of new and applied technol-ogies rdquo says Mr

Booth Mr Booth also serves on the

board of AACC International since September 2009

As par t of the in tegr at ion of Newport Scientific i n t o Pe r t e n I n s t r u m e n t s the legal entity Newport Scientific P t y L t d h a s changed name and is now Perten Instruments of Australia Pty Ltd

The company will continue to provide sales applications and service suppor t for the full range of Swedish and Australian made Per ten products Their focus on local manufactur ing and development of analyt-ical equipment such as the RVA and dough LAB remains unchanged

Mo re in fo rMatio n

Jeff RogersManaging Director Perten Instruments of Australia Pty LtdUnit 1 2 Apollo Street Warriewood NSW 2102 AustraliaTel +61 2 99796992Fax +61 2 99796993Email jrogerspertencomWebsite wwwpertencom

Perten Instruments appoints managing director in Australia

Jeff Rogers Rodney Booth

4 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 5

Aqua News

ILDEX Vietnam 2010 is ready to offer technology and business solutions for Vietnamrsquos live-

stock dairy meat-processing and aquaculture industries according to NEO - the organizer of ILDEX Vietnam 2010 which will take place on March 25-27 2010 at the New Saigon Exhibition and Convention Center Ho Chi Minh City Vietnam

NCC Exhibition Organizer Co Ltd (NEO) announced the read-iness of ILDEX Vietnam 2010 which will take place on March 25-27 2010 at the New Saigon Exhibit ion and Convention Center in Ho Chi Minh City Vietnam

At the Kick-off Launching of ILDEX Vietnam 2010 Ms Ladda Mongkolchaivivat general manager NCC Exh ib i t ion Organizer (NEO) told industry representative that ldquoILDEX Vietnam 2010 will be the largest livestock exhibition in Vietnam It will be a technology showcase of 125 companies from 22 coun-tries including country pavilion from France Singapore Korea China Shanghai City and the Netherlands

ldquoA number of international governmental and industr ial bodies such as Korean Animal Health Products Association Canadian Swine Association

British Pig Association Vietnam Depar tment of Animal Health V i e t n am Depa r tmen t o f Livestock Production and CCPIT Shanghai have confirmed their par ticipation at this industrial eventrdquo

Wi t h heav y p romot i on campaign and full government suppor t especially from the Ministry of Agricultural and Rural Development of Vietnam NEO believes that ILDEX Vietnam 2010 will attract a lot of traders and buyers from over 46 cities and provinces surrounding Ho Chi Minh City Free shuttle service will be provided for trade visitors from those areas

ILDEX Vietnam 2010 comes in perfect timing as Vietnam live-stock dair y meat-processing and aquaculture industries are searching for new technology and business solutions to move the industry to the next level

Aiming to provide the industry representative with clearer perspective of Vietnam live-stock and aquaculture indus-tries ILDEX invited Professor Hoang Kim Giao director of depar tment of livestock pro-duction Ministry of Agriculture and Rural Development and Mr Nguyen Van Trong vice director-general Aquaculture Research Institute II of Vietnam to give

keynote speeches on ldquoHusbandry in Vietnam ndash Current Status and Development Goalsrdquo and ldquoOver view Aquaculture in Vietnamrdquo respectively

Professor Hoang Kim Giao under lined the impor tant of Vietnam livestock industry

However he said ldquoIt is time for Vietnam to change the struc-ture of its agriculture and rural development Priority should be given to development of live-stock industry including industri-alization of livestock farming and food production management to produce high-quality livestock products that meet international food safety standard and enough to serve rising domestic and export demands Equally impor-tant is to protect the natural envi-ronment for sustainable develop-mentrdquo

Mr Nguyen Van Trong echoed the needs for technology and modern business solutions for Vietnam aquaculture industry Although fish and shrimp have been growing rapidly both have been facing continuous challenges especially rising production costs lowering prices and disease man-agement

ldquoOur aquaculture industr y might be growing a little too fast especially for catfish farming

ldquoThe industr y must ser i -

ously look into sustainable fa rming management Th i s t ime around the emphasis should be on quality rather than quantity to ensure that growth will continue at sus-tainable pace Pangasius for example we are having market problems so while expanding into new markets we need to be cer tain that Vietnamrsquos products meet safety standards and food hygiene required by impor tersrdquo

Apar t from technology and business solutions on the exhi-bition floors ILDEX Vietnam Aquaculture Conference 2010 under the theme lsquothe new dimen-sions for Vietnam Aquaculture Industryrsquo will be a good educa-tion forum for stakeholders along the production chain from pondhatchery operators to technical level personnel to managers feed manufacturers government officers to grasp the issues facing by the industry and develop suitable policies and aquaculture suppliers supporting the industry

Mo re in fo rMatio n

ILDEX Vietnam 2010 and ILDEX Vietnam Aquaculture Conference 2010Mr Lertwat Chanthatarath Senior Sales Manager Tel +66 2 20342614Email lertwatQsncccomWebsite wwwildexcom

ILDEX Vietnam 2010 the solutions for Vietnam sustainable growth

4 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 5

Aqua News

AE2010 is organised by the European Aquaculture Society and hosted by

the Portuguese Marine Sciences Associate Laboratory CIMAR (CIIMAR Por to and CCMAR Faro)

Portugal and PortoAquaculture production in

Por tugal is modest with some 10000 tonnes of production of which more than half is shell-fish

Marine aquaculture in Portugal is mainly centred on the Algarve coast but more recently land-based production of turbot and other marine species has devel-oped on the Atlantic coast

Currently there is renewed interest in developing aqua-culture in Por tugalrsquos outer-most regions notably in the Azores The Portuguese remain Europersquos highest consumers of fish and shellfish with annual per capita consumption of close to 60kg

Por to is Por tugalrsquos second biggest city and the lsquocapital of the nor thrsquo Its historic centre was declared a World Heritage Site by UNESCO in 1996 and it shared the designation European Culture Capital in 2001The city has excellent flight connections with flag and low cost carriers and road connections with Galicia

and especially with Vigo just 90 minutes away

AE2010 will take place at the Centro de Congressos da Alfacircndega ndash the old customs house on the quay of the Douro River in the heart of Porto and just opposite the famous por t wine cellars that are synonymous with this lively city These will form part of the AE2010 social pro-gramme

An extensive conference programme

The thematic sessions take place each morning of AE2010 and include plenary presentations by invited speakers

They pave the way for the parallel technical sessions of con-tributed presentations in both oral and poster format

A vision for the future status of marine aquaculture in Europe effects of a changing environment on biodiversity and physiology of farmed species Changing envi-ronment is at the heart of the European concerns what will be the impacts at short and medium terms For aquaculture pro-ducing specific species not able to maintain a constant body tem-perature (ectotherms) various influences are expected both for species geographical distribu-tion and so adequacy between species and present rearing sites

and impacts on species physi-ology The European aquaculture in mainly producing molluscs and fishes rapidly developed in several countries in taking into account the interdependences between species physiological needs and local conditions in terms of both more or less sheltered areas and environmental factors changes In a situation of rapid environmental changes what are the measures we have to take to follow them maybe to forecast them and to better prepare the aquacul-ture of tomorrow This review proposes to analyse this new sit-uation Presented by Gilles Boeuf President of the French National Museum of Natural History

A license to produce In the light of the new Commission strategy for the development of European aquaculture what type of gover-nance and regulatory framework is needed What should be the roles of public authorities and NGOrsquos in the development of the sector Presented by Javier Ojeda Apromar Spain

Competing claims ndash joint solu-tions How to integrate aquacul-ture with other coastal activities how can aquaculture contribute to the maritime strategy to coastal zone management and to Natura 2000 objectives Presented by Franccedilois Simard IUCN Global Marine Programme

Consumer demands and con-sumption trends What are the demand and consumption trends and what do European con-sumers prefer How important to consumers is the origin and certification of aquatic products produced lsquolocallyrsquo in Europe or impor ted from third coun-tr ies Presented by Philippe Paquotte DG Mare European Commission

Sustainable sea farming How do we develop sustainable sea farming using the ecosystem approach How is aquaculture linked with natural ecosystem processes and with patrimonial cultivation Presented by Joatildeo Gomes Ferreira New University of Lisbon Portugal

AE2010 is now calling for abstracts for presentation in parallel and poster sessions

These sessions are organised in several groups as follows

bull Hatchery production and spec ies d iver s i f i ca t ion including sessions on

bull Finfish hatcher y produc-tion covering all aspects of juvenile production proc-esses and management

bull Shellfish (juvenile) produc-tion including new develop-ments in breeding hatchery production natural spat col-lection

bull Management of aquaculture

The future of marine aquaculture

Aquaculture Europe 2010 to be held in Porto Portugal from October 5-8 2010 puts the focus on the future of marine aquaculture in Europe

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

stocks including reproduc-tion breeding and genetic improvements

bull Species diversification - finfish including the culture of flatfish tuna and other lsquonewrsquo finfish species for future commercial production

bull Species diversification ndash other species including the culture of octopus cuttlefish abalone crabs seaweeds and other lsquonewrsquo species for future commercial production

bull Production in different envi-ronments and produc-tion technologies including sessions on

bull Land-based production - new production technol-ogies including large-scale RAS production in ar id regions and other land based activities

bull Offshore aquaculture of fish and shellfish

bull Aquaculture on Islands ndash specificities of production in outlying areas in tropical and temperate regions including European outer-most regions

Tools for the development of the sector including sessions on

bull The eco-system approach to aquaculture produc-tion including semi inten-siveextensive coastallagoon aquaculture nutrient man-agement and IMTA invasive species predator control nature management and pat-rimonial conservation

bull Space for aquaculture Carrying capacity and Spatial Planning

bull Aquaculture governance and interactions with fish-eries including policy soci-oeconomic and economic research and interactions commonalities and interde-pendence with fisheries

bull Health quality and well-being including sessions on

bull Human health consumer science and quality including

aquaculture percept ion studies biotoxins quality assessment and detection certification

bull Health management of finfish and shellfish Sponsored by Inter vet Schering-Plough Animal Health

bull Novel feeds ingredients and additives Sponsored by Biomin

bull Aquaculture welfare and ethics

The conference will also include a special Student Workshop and naturally a student reception

Science meets industryAquaculture Europe 2010 will

also feature a special interna-tional trade exhibition organised by the European Aquaculture Society Por tuguese Spanish and International companies will present the latest products and ser vices for aquacul-ture A special farmersrsquo day programme will have an emphasis on aquaculture in Por tugal and in Spain and will focus on topics where the latest research findings can have an impact on the sector

It is hoped that the main Por tuguese and Spanish industr y associations for finfish and shel l fish wil l take advantage of the farmersrsquo day to bring their members to Por to and to use the event for their own meetings

Good local and industry support

AE2010 i s f inanc ia l ly suppor ted by the Area Metropolitana do Porto

The gold sponsor of AE2010 is Sorgal SA

E A S i s p r o u d t o announce the AE2010 Gold Sponsorship of Portuguese a q u a f e e d c o m p a n y Aquasoja Aquasoja is the fish feed brand of Sorgal SA Sorgal SA is par t of a

large Por tuguese agro-indus-trial group Sorgal has produced quality feeds for animal farming for more than 50 years and has always conducted its activities focussing on cost-effectiveness animal welfare and feed safety as top priorities

As par t o f i t s Premier Sponsorship of the European Aquaculture Society Inter vet Schering Plough Animal Health will have a strong presence and will sponsor the health manage-ment session

Feed ingredients and addi-t ives specia l i s t B iomin wi l l sponsor the session on Novel feeds ingredients and addi-tives

Those interested in booking exhibition space andor sponsor-ship opportunities should contact Mario Stael at mariostaelscarletbe

EU to showcase FP6 research

The EU Forum ndash a regular feature of Aquaculture Europe events ndash is currently being planned and will look to showcase the results of two of the biggest integrated research projects carried out by the Commission in aquaculture as well as presenting the view of national research funders The EU Forum will also look forward to the final stages of FP7

Just pr ior to AE2010 the Larvanet network will meet in Porto The latest findings of their research will be presented in a special session within the confer-ence A series of short workshops organised by other EU-funded projects and other relevant ini-tiatives will also take place in the exhibition area and specifically targeted towards exhibitors and trade show visitors

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

Kiotechagil the interna-tional supplier of natural high performance feed

additives to enhance growth health and sustainability in agri-culture and aquaculture will have a strong presence at VIV in The Netherlands in April 2010

The company will be high-lighting their new acidifier pHorce which compliments their range of acid products pHorce will provide a concen-trated acid on a micropearl silica carrier and can be used for decontamination of raw materials as an alternative to

c o r r o s i v e liquids and in finished feed at one kgtonne

In addition Kiotechagil will be showcasing a new broad spectrum anti-mycotoxin product which will combine the benefits of their highly effective alumino-silicate Sorbatox with a primary growth yeast cell wall compo-nent high in both beta glucans and mannan oligosaccharides

Mo re in fo rMatio n

Kiotechagil VIV Stand No C066 in Hall 9Tel +44 1189 813333Email infokiotechagilcom

Kiotechagil to show pHorceful presence at VIV

Animal nutrition company Norel launched a new website wwwnorel

es that will provide up-to-date information about its products and the company activities

Founded in 1980 by Mr Enrique Pablos Perez Norel SA has been steadily growing and developing into a well known brand The original meaning of Norel was the Spanish phrase lsquoNO REconocemos Limitesrsquo l i teral ly meaning lsquoWe donrsquot acknowledge limitsrsquo thus dem-onstrating Norelrsquos philosophy The Skyrsquos The Limit or in other words the possibi l i t ies are endless

Norel has become associated with dynamism and innovation (more than five percent of its turnover is invested in Research and Development) and also with quality and performance thanks to our ISO GMP and FAMI-QS certifications

An important feature of Norel is its focus since the beginning on animal nutrition

Originally Norel was a supplier of raw mater ials for animal nutrition milk replacers whey products and protein extracts In 1982 it star ted the production

of spray fat concentrates as well as organic trace minerals (methionates)

Expansion and develop-ment followed and in 1988 Norel opened i ts b io-technology plant in Leon (Spain)

ldquo We t h e n b e c a m e the fi r st b iotechnology company in Spain spe-cialised in animal nutrition In 1990 we star ted the production of by-pass fats for ruminants Today with our flagship Magnapac we are one of the major pro-ducers worldwide Our devel-opment continued with new factories in Egypt and Mexico and specialized units in Spainrdquo says the company in an inter-view with the press

ldquoAn impor tant date in our history is certainly 2001 when Norel SA merged its activities with Nature SA a company spe-cialized in the production of flavours and sweeteners acidi-fiers antioxidants and mould inhibitors

ldquoWe then became known in the market as lsquoNorel amp Naturersquo at that time it was impor tant for us to keep the name Nature

because our act iv it ies and products were different but at the same time very complemen-tary Today we are pleased to introduce our new logo together with the launch of our brand new website wwwnoreles

The new logo of Norel takes into account the histor y by combin ing the two Nrsquos of Norel and Nature Together they resemble the famous Yin Yang symbol and remind us that the two entities now make one The new logo also represents the infinity symbol a reference to the or ig inal meaning of Norel continue to

grow and the possibilities are endless

I t s new webs i t e comes w i th an improved cor po-rate identity and many new features and updates Most impor tant ly i t has added a news section that wil l keep reader s updated on la test developments and activ it ies of Norel worldwide

Mo re in fo rMatio n

Norel SAJesus Aprendiz 19 1 A y BMadrid NC 28007 SpainTel +34 6698 09659Website wwwnorelnaturecom

NOREL announces new web site and new corporate image

8 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 9

Dishman Group o f Ahmedabad I nd i a a lead ing products

and ser vices provider to the pharmaceutical industr y has announced the appointment of Chris Oates to the position of chief operating officer

Mr Oates will repor t to Nick Green president of the Dishman Globa l Contr act Research and Manufactur ing Ser vices (CRAMS) business

M r O a t e s w i l l a s s u m e responsibil ity for Indian and Chinese operations within the company

He wi l l be managing the development operation and improvement of the systems in the two manufacturing sites in India (Bavla and Naroda) and

the new Shanghai facility which is due for completion mid-2010

In addition to the units in Naroda and China Mr Oates wil l be responsible for the nine operating units at Bavla including a large-scale high potency fac i l i ty which wi l l be inaugurated at the end of January The new state-of-the-ar t high potency facil ity will extend Carbogen Amcisrsquo man-ufactur ing capacity to multi-tonne scale and will be the first of its kind to be located in Asia

M r O a t e s rsquo e x p e r i e n c e combines both product ion and operational roles in the contract manufacturing arena He will play a pivotal role in

expanding the companyrsquos core CRAMS business

Mr Oates holds a bach-elorrsquos degree in applied chem-istr y from the University of Huddersfield in the UK His exper t ise is bui lt on more than 20 years working within the pharmaceutical industr y He most recently ser ved as managing director of Uquifa Mexico His previous expe-r ience includes six year s at Avecia Pharmaceuticals in the UK where he held the position of operations manager and as the position of senior pro-duction manager for Syngenta (Agrochemicals)

ldquo I am looking forward to taking up this new challenge and am very excited by what I

believe will be a very successful future for the companyrdquo said Mr Oates

ldquoDishman can provide the full continuum of ser vices from process RampD to large-scale commercial manufacturing in multiple locations around the globe The company is ideally p l aced to bu i ld upon i t s success to date and become the industr y leading CRAMS par tnerrdquo

Mo re in fo rMatio n

Dishman Europe LimitedSuite 4 De Walden Court 85 New Cavendish Street London W1W 6XDUnited KingdomTel +44 2073 230608 Fax +44 2073 230609Website wwwdishmangroupcom

Dishman appoints Chris Oates as chief operating officerNew role will oversee Dishman Indian and Chinese Facilities

Sponsorship of a keynote session at Aquaculture 2010 confirms Biominrsquos

firm commitment and keen focus on this dynamic growth sector

Biomin embraces the new year with a bold plunge in aqua-culture

Fo r the f i r s t t ime the company wil l be par tner ing the World Aquaculture Society (WAS) with the sponsorship of the Aquafeeds Session at Aquaculture 2010

To be held in San Diego from March 1-5 2010 Aquaculture 2010 is the largest event for the global aquaculture industry Hosted every year by the WAS the global aquaculture event is expected to draw some 4000 participants from over 80 coun-tr ies including high profi le

attendees who form the core of decision-makers and innova-tors in the aquatic business

Biomin is proud to be a session sponsor at the worldrsquos largest aquaculture event Held on March 4 2010 the session on lsquoAquafeedsrsquo will see exper t speakers from com-mercial practice and academia share their i n s i gh t s on ma jor issues in aquafeeds formulation and alter-nat ives to fishmeal and protein replace-ment strategies for a variety of aquatic species

Years of dedicated applied research and investments have strengthened Biominrsquos drive and reputation in providing effective natural-based feed solutions for the aquaculture and aquafeed

business throughout the world A core focus on fundamental applied research forms the basis of Biominrsquos portfolio of science-based aquaculture product solu-tions Biominreg AquaSpecials which includes nutraceuticals probiotics natural growth pro-

moters mycotoxin deactiva-tors and functional feed addi-tives

Dr Pedro Encarnaccedilatildeo Biominrsquos aquacul ture spec ia l i s t says ldquoBiomin is a research-focused and -driven company

ldquoWe have translated our long-

standing competence in animal nutrition for terrestrial species into aquaculture and aquafeed research Our Aquatic Centre for Applied Animals (AQUACAN) in Thailand actively conducts feeding trials for many aquatic species from which new findings

have been translated into innovative and practical solutions in aquafeedsrdquo

Biomin will also par-ticipate as a sponsor at the Australas ian Aquacu l ture 2010

another WAS event to be held in Hobar t Australia in May 2010 and at the Aquaculture Europe that will take place in Por to Por tugal in October 2010 both of which are key events for aquaculture in the coming year

A bold step for BIOMIN aquacultureBiomin proud to be session sponsor at the worldrsquos largest aquaculture event

8 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 9

Aqua News

Tecnoloacutegica de Alimentos SA (TASA) of Peru is the first fishmeal and fish

oil producer to achieve cer ti-fication under the new IFFO Global Standard for Responsible Supply

The cer tification has been awarded to the Callao Nor te plant of TASA the worldrsquos largest fishmeal producer Earlier this week Jorge Mora Operations Director of IFFO presented the

IFFO-Assured certificate to Mario Brescia CEO of TASA at its head-quarters in Lima Peru

IFFO has been developing its RS Standard since early 2008 with the help of a Technical Advisory Committee comprising repre-sentatives from the whole aqua-culture value chain including fish farmers feed producers and retailers and also NGOs

This business-to-business inde-pendently-audited programme will enable IFFO members to demonstrate to their customers that they are offer ing pure marine ingredients manufactured from responsibly sourced and traceable raw materials Whole fish used must come from fish-eries managed under the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

ldquoSince October 2009 when we launched the programme we have had applications from 15 manufacturing companies in four major producing countries sourcing from 10 fisheriesrdquo says IFFO director general Jonathan Shepherd

ldquoWe anticipate that there will be substantial supplies of IFFO-Assured fishmeal and fish oil on the market within months and that up to one third of global

p r o d u c t i o n could be certi-fied by the end of 2010rdquo

ldquoWith th i s p r o g r a m m e IFFO has put in place the first link in a fully certified supply chain for aqua-culture and for land animals such as pigs and poul tr y

It will help the industry demon-strate its commitment to respon-sible manufacture of fishmeal and fish oil as well as to the FAO Code of Conductrdquo said Dr Shepherd

Applicant factories which can demonstrate that they are ldquooper-ating a well managed system that ensures their marine raw mate-rials are responsibly sourced and processed to the IFFO Standardrdquo will be awarded certification

They will be able to offer IFFO-Assured fishmeal and fish oil and have the right to display the RS logo An up-to-date list of cur-rently certified fishmeal plants will be posted on the IFFO web site alongside the full Standard

Characteristics of the IFFO Global Standard for Responsible Supply (RS)

bull Standards set by a multi-stake-holder Technical Advisory

Committee including pro-ducers traders fish farmers feed manufacturers proc-essors retailers standard setting organisations and environmental NGOs The Assessment Management and Certification Process are contracted to Global Trust

bull A programme independ-ently administered by an ISO 651996 accredited certifica-tion body which is a member of the International Accreditation Forum (IFA)

bull I t d e m o n s t r a t e s responsible sourcing o f t r aceable r aw material and respon-sible production to ensure feed safety

bull A business-to-busi-ness tool designed to enable fishmeal and fish oil producers to demonstrate the integr i ty o f the i r products and their processes to their cus-tomers in the value-chain rather than a consumer eco-label

bull Whole fish used must come from fisheries that have been independently assessed and meet the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

bull Exclusion of illegal unre-por ted and unregulated (IUU) fish

bull The factor y must have attained Cer tification to the Internat ional Feed Ingredients Standard (IFIS) of the International Feed Safety Alliance (IFSA) or equiva-lent as proof of responsible manufacturing and the appli-cant must be in possession

of all relevant permits and licenses for the production and sale of fishmeal and fish oil products

bull This programme is com-patible with ISO 651996 (General requirements for bodies operating product cer tification systems) and with EN45011

bull Recogn i ses other cer-t i f i c a t ion p rog r ammes w h i c h h av e d e m o n -

strated equivalency and which are accepted within the industry - eg Marine Stewardship Council (MSC) for the fishery certification element only

The IFFO programme will continue to be developed in 2010 addressing further aspects of responsible supply

Mo re in fo rMatio n

Andrew JacksonTechnical Director IFFO 2 College YardLower Dagnall Street St Albans Hertfordshire AL3 4PAUnited KingdomTel +44 7808 783488 Email ajacksoniffonetWebiste wwwiffonet

First certification awarded under the IFFO Global Standard for Responsible Supply

ldquoThe first link in a fully certified aquaculture supply chain is in placerdquo

ldquoSupplies of ldquoIFFO-Assuredrdquo fishmeal and fish oil will shortly be on the marketrdquo

Jorge Mora (right) Operations Director of IFFO presenting the IFFO-Assured certificate to Mario Brescia (left) CEO of TASA at its headquarters in Lima Peru

10 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 11

Me r c a t o r M e d i a o r g a n i s e r o f t h e Offshore Mariculture

Conference 2010 is pleased to announce that the leading i ndus t r y a s soc i a t ions w i l l be suppor t i n g t he 2010 Conference

The European Aquaculture Techno logy amp I nnova t ion Platform (EATiP) the Federation o f Eu ropean Aquacu l tu re Producers (FEAP) the Global

Aquaculture All iance (GAA) and HGK Grupacija akvakul-ture the aquaculture affi l ia-tion of the Croatian Chamber of Economy (and member of FEAP) have all pledged their suppor t to the Conference that wi l l be held from the

June 16-18 2010 at the Hilton Imper ial Hotel Dubrovnik Croatia

Both EATiP (European Aquaculture Technology amp Innovation Platform) and FEAP (Federation of European Aquaculture Producers) are pleased to suppor t the Offshore Mariculture Conference s ince events such as these give fish farmers t h e o p p o r t u -n i t y t o l e a r n from both indus-t r i a l and RTD

exper ts about relevant technological advances to rece ive in formed updates on key research and policy issues and

to be able to network with col leagues from across the sector says Cour tney Hough general secretary of FEAP and EATiP

As wel l as promoting the event to their members and therefore rais ing awareness of the event and its presti-gious speakers it also means that members of any of the above assoc ia t ions wi l l be en t i t l ed to a 10 pe rcen t d i s count o f f the de lega te rate European Aquaculture Society members will also be ent i t led to the discount as the EAS have continued their suppor t f rom the prev ious even t he ld i n A l i c an te i n 2008

The technical conference will be held on the June 16 and 17 June with a visit to a fish farm on the June 18 2010 The Conference Networking Dinner will take place on the evening of Wednesday June 16 2010 at the fabulous Revelin For tress which was bui lt in 1462 to provide protection to the Eastern City Gate Place Over look ing the Old Ci ty Harbour the Revelins terrace offers what are probably the best views of the Old City and its famous Harbour

Mo re in fo rMatio n

Offshore MaricultureTel +44 1622 820622Website wwwoffshoremariculturecom

Industry support strengthens for 2010 OSM Conference

The full programme will be released shortly sessions will include

bull Policy legal framework and key research updates

bull Practical offshore farming operation

bull Spatial planning and moni-toring

bull Spotlight on Croatiabull Ecological monitoring and

future technologiesbull European and International

case studies

iN THE NEWSYour news could feature in our pages

Do you have an news item that you would like to see in our pages Send your press releases to the Production Manager - Nicky BarnesEmail nickybaquafeedcouk

10 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 11

Aqua News

The aquaculture industry today faces a number of challenges that threaten to restrict its development

and profitability

To live in harmony with consumer expec-tations of the highest possible standards of product quality and to do so in a sustainable and environmentally responsible manner necessitates an innovative coordinated and holistic approach within the aquaculture industry

The requirement for sustainability has focused on the composition of the feed being used as one of the key parameters determining the sustainability of production of a particular species particularly the fish-meal and fish oil content and how it relates to overall feed conversion ratios

Further formulation trends are moving towards low fishmeal and oil content in the grower feeds and finishing feeds designed to restore flesh quality parameters such as omega 3 fats and fillet quality that are con-sidered essential for the promotion of the product and its benefits for human health

This strategy addresses to some extent the limited resources of fishmeal and fish oil

Expectations of sustainabilityThe only way to achieve the expecta-

tions of sustainability and replace or supple-ment fishmeal and fish oil in aquafeeds is to exploit alternative protein and lipid sources

The obvious and traditionally used alter-natives are animal and plant protein sources

However in Europe and now increas-ingly in North America consumers pre-fer aquaculture products to have been

fed without the use of animal by-products due in part to recent health scares in the terrestrial animal sec-tor such as bovine spongiform encepha-lopathy (BSE)

The search is on therefore for not only the simple replacement of protein but the provi-sion of functional nutri-tion addressing both the sustainability and flesh quality requirements of the industry while

meeting the nutritional requirement of the animal being cultured

Alternative vegetable protein sources

Over the last two decades aquaculture research has focused on plant protein sources such as grains pulses and oilseeds as they are generally widely available

However the use of soybean meal and other plant based protein sources such as legume seeds oilseed cakes leaf meals leaf protein concentrates and root tuber meals have been limited due to the presence of a wide variety of anti-nutritional substances in these products and their interaction with the physiology of the animal resulting in depressed growth and feed efficiency mortality and abnormal intestinal morphol-ogy and damage

These substances act in different ways by affecting protein utilisation and digestion (eg protease inhibitors tannins lectins) by affecting mineral utilisation (eg phytate gossypol pigments glucosinolates) antivita-mins and other miscellaneous substances (eg phytoestrogens)

The increasing use of plant protein sources is also raising new concerns about possible contamination by mould derived mycotoxins

This risk is enhanced by the global nature and trading of plant protein sourc-es and the as yet little investigated impact of different mycotoxins and their interac-tions in aquaculture species In 2008 the Rapid Alert System for Food and Feed in

Protein sources Alternative protein sources should also show functionality if they are to play a role in modern aquafeedsby Keith Filer PhD research Manager Asia-Pacific biosciences Centre Alltech inc

Figure 1 A comparison of typical essential amino acid (EAA) requirements for fish and the EAA profile of AquateTM

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

juns

wor

th |

Dre

amst

ime

com

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

040

120

08

180

120

08

010

220

08

150

220

08

290

220

08

140

320

08

280

320

08

110

420

08

250

420

08

090

520

08

230

520

08

16 | InternatIonal AquAFeed | March-april 2010

Alltechreg has developed a range of products to address dietary requirements at different

stages of life in aquaculture species These sustainable product solutions are proven to

support profitability in aquaculture operations as well as performance in fish and shrimp

in the Family

Give your fish and shrimp the Alltech Aqua Advantage Contact your Alltech representative today for more information

trade

aquasolutionsalltechcomwwwalltechcom

Ideally suited for fish and shrimp in any stage of life to maintain performance and to support independence from traditional aquaculture diet ingredients

Specifically designed for the juvenile stages of life in fish and shrimp to support good health uniform growth and survival

A natural affordable product created for salmon in all life stages to maintain good health and defense mechanisms against adverse aquatic elements present in the surrounding environment

trade

trade

Aquate Ad for IAF due Feb 19 2010V2indd 1 2192010 115722 AM

Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

8 page full colour document - 100 copies = UKpound489 (please add UKpound15 for every additional 100 copies)

Delivery charged on a case by case basis

For more details please visit wwwaquafeedcoukreprints

1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

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20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

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19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

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5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

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8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

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6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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T +49 2215 7993693 | F +49 2215 799389

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International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

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International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

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Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

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Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

  1. Button 1
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Page 2: March | April 10 - International Aquafeed

Volume 13 Issue 2 March-April 2010 copy Copyright Perendale Publishers Ltd 2010 All rights reserved

Aquaculture UK 2010 19-20 May 2010 at Aviemore

Now an established major event Aquaculture UK will be held again in May 2010With visitors from 18 countries as far afield as Sweden Chile and the United States

make sure you donrsquot miss out on this opportunity to connect with buyers and decision makers from the aquaculture industry worldwide

Herersquos what exhibitors said of the 2008 exhibition

ldquoAviemore was a good location and the show had a certain spirit which is always hard to createrdquo Guy Mace - MD Biomar

ldquoThe show went well for EWOS - our costs were much lower than Glasgow and we got the audience we wantedrdquo Douglas Low - MD Ewos

ldquoThe feedback regarding Aviemore seems to have been very positive and it had a much better atmosphere than GlasgowrdquoWill Jewison - Skretting

ldquoMany thanks for delivering a very successful exhibition and return to Aviemorerdquo Alan Stewart - Director Landcatch

aquaI n t E r n A t I o n A l

feedAn internAtionAl mAgAzine for the AquAculture feed industryCONTENTS

PerendalePublishers Ltd International Aquafeed is published six times a year by Perendale Publishers Ltd of the United Kingdom

All data is published in good faith based on information received and while every care is taken to prevent inaccuracies the publishers accept no liability for any errors or omissions or for the consequences of action taken on the basis of information published copyCopyright 2010 Perendale Publishers Ltd All rights reserved No part of this publication may be reproduced in any form or by any means without prior permission of the copyright owner Printed by Perendale Publishers Ltd ISSN 1464-0058

Volume 13 Issue 2 March-April 2010 copy Copyright Perendale Publishers Ltd 2010 All rights reserved

EDITORrsquoS DESK 2

Aqua NewsA bold step for BIOMIN aquaculture 3Strategic Cooperation Agreement between CPF and AKVA 4Perten Instruments appoints managing director in Australia 4ILDEX Vietnam 2010 the solutions for Vietnam sustainable growth 5The future of 6marine aquaculture 6NOREL announces new web site and new corporate image 8Kiotechagil to show pHorceful presence at VIV 8ARCHIVE 8Dishman appoints Chris Oates as chief operating officer 9New role will oversee Dishman Indian and Chinese Facilities 9First certification awarded under the IFFO Global Standard for Responsible Supply 10Industry support strengthens for 2010 OSM Conference 11

ProteinProtein sources - Alternative protein sources should also show 12 functionality if they are to play a role in modern aquafeeds

ProbioticEvaluation of probiotic bacteria in prawn production 18

THE AQUAFEED PHOTOSHOOT 24

ExtrusionProtein damagecomplexation during extrusion 26

VacuumA vacuum batch coating system especially designed for aquafeed and pet food 32

AquafeedsAquafeeds ready to take the plunge 34

Feed ManagementAquafeeds in Tanzania 36Diets for tilapia brood stock Addressing challenges for reducing dependency on fish oil 38

Book Reviews 42

CLASSIFIED ADVERTS 44

IAF WEB LINKS 46

AQUA EVENTS 48

Cov

er im

age

cour

tesy

of

Fusi

on M

arin

e Li

mite

d

EDITORrsquoS DESK W

ELC

OM

E TO

INTE

RN

ATIO

NA

L AQ

UA

FEED

MA

GA

ZIN

E

Croeso (Welcome)

As we leave a very cold dark winter behind us in Northern Europe and the USA we look towards the glimmer of spring and the start a new produc-tion cycle in the Northern Hemisphere Of course this contrasts with the tropics where demand for fish and shellfish production is expanding and

the aquafeed sector must respond accordingly

SE Asia continues to dominate and it is not surprising that International Aquafeed continues to receive invaluable contributions from specialists in this region

I am delighted to overview the contribution of Keith Filer from Alltech Asia ndashPacific concerning the development of novel proteins based on single cell sources such as yeasts and algal blends and how this might contribute significantly to diet formulations for many fish species in future

He focuses on some recent scientific appraisals of products from both a production basis and promotion of health

This fits in with the overall global sustainability agenda and is further elaborated by Daphne Tanrsquos feature which takes a more holistic view of the protein and feed ingredient needs in aquaculture and addresses this topic in an interesting approach from Singapore with an expert eye on the market trends and potential for raw material usage

My editorial associate Professor Krishen Rana from Stirling again addresses feed management issues with his sound knowledge of Africa and this time turns his attention to Tanzania and tilapia and in particular follows with a specialised focus on tilapia brood stock diets

Indeed we must in future include a special feature on the nutritional requirements of brood stock fish in general as the aquafeed industry always needs to produce a range of special feeds for shrimp maturation and large mature fin-fish entering their reproductive stages

As such feed management is an important aspect of good husbandry and I thank Krishen for his excellent contributions to this section and wide applications to effective feed delivery and efficiency gains

The technical issues relating to feed manufacture are highlighted in an article concerning protein damage and stability in the complex extrusion process This is a very important concern and is per-tinent to our understanding of the delicate nature of many feed ingredient and how their nutritional value can be modulated under various conditions We also note the importance of post-extrusion vacuum coating technology for specialised feed additives and oil inclusion

Finally I mention a feature from one of our youngest contributors Tim OrsquoBrine who at 22 is one of my MSc students undertaking his Masters in lsquoSustainable Aquaculture Systemsrsquo

His article reviewing the use of probiotics in prawn production is comprehensive and a useful staging post for further consideration on disease and health management of shrimp

I am sure you will enjoy this spring issue next time I will report directly from the World Aquaculture Society meeting in San Diego and which will no doubt provide many new topics for discussion

March-April 2010

SubSCribEwwwaquafeedcouksubscribephp

International Aquafeed is published six times a year bringing you in-depth features industry news events book reviews and more Subscribers to International Aquafeed also receive a free copy of the International Aquafeed Directory worth UKpound85 For more information please visit our website

2 | InternatIonal AquAFeed | March-april 2010

EditorProfessor Simon DaviesTel +44 1242 267706Email simondaquafeedcouk

Associate EditorProfessor Krishen RanaTel +44 1242 267706Email krishenraquafeedcouk

Editorial Advisory Panelbull Abdel-Fattah M El-Sayed (Egypt)bull Aliro Borquez (Chile)bull Chen Shuping (China)bull Citas Pascual (Philippines)bull Colin Mair (UK)bull Daniel Montero (Spain)bull Dom Bureau (Canada)bull Eric De Muylder (Belgium)bull Miguel A Olvera (Mexico)bull Mike Cremer (USA)bull Ola Flesland (Norway)bull Mohammad R Hasan (Italy)bull Ming DanChen (Thailand)

International Marketing Manager

Caroline WearnTel +44 1242 267706Email carolinewaquafeedcouk

Subscription amp Circulation Manager

Tuti TanTel +44 1242 267706 Email tutitaquafeedcouk

Production Manager

Nicky BarnesTel +44 1242 267706 Email nickybaquafeedcouk

Design amp Page Layout

James TaylorTel +44 1242 267706 Email jamestaquafeedcouk

2 | InternatIonal AquAFeed | March-april 2010

Tel +1 314 739 9191 bull +1 800 288 2726 bull Fax +1 314 739 5880 bull wwwtapcoinccomSt Louis Missouri USA

ELEVATOR BUCKETS - ELEVATOR BOLTS

Jim Cunningham USA

(Speaks English)

ldquoRounded edges =

gentle handlingrdquo

Wherever your product is processed storedor shipped one thing is constantthe

need for high quality buckets thatstand the test of time

Since 1974 Tapco buckets havebeen outperforming all othersin tests and actual usage ndashhandling everything from

feed to fertilizer with care andefficiency Maybe thatrsquos why

Tapco has the two most popular designs in the world Tapco hasa bucket that will interchange and replace existing buckets to giveyou the durability you need to handle abrasive loadshellipwith equal or greater capacity

When you call Tapco yoursquoll be pleased to learnthat your representative literally DOES speakyour language ndash and knows which bucketsare the best choice for your application Visitwwwtapcoinccom to find out moreabout Tapcorsquos world-class features

Thorsten Rogner Europe amp Asia(Speaks German)ldquoCC-XD ndash Toughest on the marketrdquo

Bruce Fagala USA (Speaks English)

ldquoNon-sparking and saferdquo

Jimi Davidson USA (Speaks Spanish)ldquoAvailable in polyethylenepolyurethane and nylonrdquo

FANGED HEADElevator Bolt

FLAT COUNTERSUNKHEAD (NO 1 NORWAY)

Elevator Bolt

Carl Swisher USA amp Canada

(Speaks Spanish)

ldquoXtremely efficientrdquo

Yassine Abbad Middle East amp Africa(Speaks French amp Arabic)ldquoBuckets for every applicationrdquo

John Paul Santangelo Latin America (Speaks Spanish)

ldquoStocking distributors worldwiderdquo

Mike Kerlagon USA (Speaks English)

ldquoSame day shipping availablerdquo

When it Comes to Elevator Bucket PerformanceTapco Speaks Your Language

reg

STYLE SUPER EUROBUCKETtrade CC-XD(XTREME DUTY) amp CC-HD (HEAVY DUTY)

Polyurethane Nylon and Polyethylene Elevator Buckets

The color blue when used in connection with elevator buckets is a US registered trademark owned by Tapco Inc Super EuroBucket trade is a registered trademark of Tapco Inc copy 2010 Tapco Increg All rights reserved

SpeakAd_FINALA4 22310 102 AM Page 1

Strategic Cooperation Agreement between CPF and AKVA

Charoen Phokphand Foods Ltd the worlds leading producer and

suppl ier of farmed White Shrimp and AKVA group ASA the worlds leading supplier of technology for the aquacul-ture industry have signed a stra-tegic cooperation agreement for developing a complete new production system for the pro-duction of white shrimp utilising AKVAs recirculation technology and CPFs broad knowledge in shrimp farming

CPF is working actively to improve the i r product ion methods and technology

A natural next step for the shrimp farming industry is to utilise new technological oppor-tunities to address many of the challenges of the industry and society today

ldquoThis strategic cooperation agreement with AKVA is an important step for us in our strive

towards a transformation of the shrimp farming industry

ldquoBy th i s pro ject we are addressing impor tant issues for the industr y such as the level of which marine resource and land areas are utilised the escaping of shrimp from farms to interfere with wild stocks the control of diseases and efficient battling of parasites the elimi-nation of and risks of pollution from shrimp farms and other possible effects on the habitats where the farming is done Further to this we believe that the technology that this stra-tegic cooperation agreement will produce has the potential also to limit the carbon foot-print of the produced shrimp significantlyrdquo says Dr Chingchai Lohanawatanakul Chairman of the executive Board of CPF

The first step of the strategic cooperation includes developing a full scale prototype of a 200

tonnesyear intensive facility for white shrimp (Litopenaeus Vannamei) as an integrated solution

ldquoFor AKVA group this is an important step forward in our development in the Asian market and the furthering of our recir-culation technology in the global market

ldquoWe appreciate the initia-tives and eagerness that CPF is showing in this project and are looking forward to the results from this cooperation for both par tiesrdquo says Mr Knut Molaug CEO of AKVA group

Mo re in fo rMatio n

Knut MolaugCEOAKVA group ASA Nordlysveien 4PO Box 271 N-4349 BryneNorwayTel +47 901 39522Fax +47 51 778501Email kmolaugakvagroupcom

Back from left Mr Robins P McIntosh (Senior VP CPF) Mr Jan Erik Svensson (Regional Director AKVA group S E Asia) Dr Chingchai Lohawatanakul (Chairman of the Executive Board CPF)Front from left Mr Knut Molaug (CEO AKVA group) Dr Sujint Thammasart (Executive VP CPF)

Je f f R o g e r s h a s b e e n appointed managing director to lead Per ten Instruments

of Australia Pty Ltd He brings over 25 years expe-

rience in the scientific instrument equipment marketplace and is well qualified to lead the company in delivering our world class tech-nology and support

ldquoThis is a very exciting time for

our company We are all looking forward to a great 2010 with our customers and suppliersrdquo says Mr Rogers

He takes over from Rodney B o o t h w h o h a s r e t i r e d a s manag ing d i r ec to r and w i l l now t a ke a Bu s i ne s s Development role within the l a r ge r Per ten In s t r ument s G r o u p R o d n e y f o u n d e d N e w p o r t S c i e n t i f i c a n d l aunched the Rap id V i s co Analyser (RVA) in 1985

ldquoI am looking forward to my new focus on strategic projects a n d r e s e a r c h a n d d e ve l o p -ment of new and applied technol-ogies rdquo says Mr

Booth Mr Booth also serves on the

board of AACC International since September 2009

As par t of the in tegr at ion of Newport Scientific i n t o Pe r t e n I n s t r u m e n t s the legal entity Newport Scientific P t y L t d h a s changed name and is now Perten Instruments of Australia Pty Ltd

The company will continue to provide sales applications and service suppor t for the full range of Swedish and Australian made Per ten products Their focus on local manufactur ing and development of analyt-ical equipment such as the RVA and dough LAB remains unchanged

Mo re in fo rMatio n

Jeff RogersManaging Director Perten Instruments of Australia Pty LtdUnit 1 2 Apollo Street Warriewood NSW 2102 AustraliaTel +61 2 99796992Fax +61 2 99796993Email jrogerspertencomWebsite wwwpertencom

Perten Instruments appoints managing director in Australia

Jeff Rogers Rodney Booth

4 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 5

Aqua News

ILDEX Vietnam 2010 is ready to offer technology and business solutions for Vietnamrsquos live-

stock dairy meat-processing and aquaculture industries according to NEO - the organizer of ILDEX Vietnam 2010 which will take place on March 25-27 2010 at the New Saigon Exhibition and Convention Center Ho Chi Minh City Vietnam

NCC Exhibition Organizer Co Ltd (NEO) announced the read-iness of ILDEX Vietnam 2010 which will take place on March 25-27 2010 at the New Saigon Exhibit ion and Convention Center in Ho Chi Minh City Vietnam

At the Kick-off Launching of ILDEX Vietnam 2010 Ms Ladda Mongkolchaivivat general manager NCC Exh ib i t ion Organizer (NEO) told industry representative that ldquoILDEX Vietnam 2010 will be the largest livestock exhibition in Vietnam It will be a technology showcase of 125 companies from 22 coun-tries including country pavilion from France Singapore Korea China Shanghai City and the Netherlands

ldquoA number of international governmental and industr ial bodies such as Korean Animal Health Products Association Canadian Swine Association

British Pig Association Vietnam Depar tment of Animal Health V i e t n am Depa r tmen t o f Livestock Production and CCPIT Shanghai have confirmed their par ticipation at this industrial eventrdquo

Wi t h heav y p romot i on campaign and full government suppor t especially from the Ministry of Agricultural and Rural Development of Vietnam NEO believes that ILDEX Vietnam 2010 will attract a lot of traders and buyers from over 46 cities and provinces surrounding Ho Chi Minh City Free shuttle service will be provided for trade visitors from those areas

ILDEX Vietnam 2010 comes in perfect timing as Vietnam live-stock dair y meat-processing and aquaculture industries are searching for new technology and business solutions to move the industry to the next level

Aiming to provide the industry representative with clearer perspective of Vietnam live-stock and aquaculture indus-tries ILDEX invited Professor Hoang Kim Giao director of depar tment of livestock pro-duction Ministry of Agriculture and Rural Development and Mr Nguyen Van Trong vice director-general Aquaculture Research Institute II of Vietnam to give

keynote speeches on ldquoHusbandry in Vietnam ndash Current Status and Development Goalsrdquo and ldquoOver view Aquaculture in Vietnamrdquo respectively

Professor Hoang Kim Giao under lined the impor tant of Vietnam livestock industry

However he said ldquoIt is time for Vietnam to change the struc-ture of its agriculture and rural development Priority should be given to development of live-stock industry including industri-alization of livestock farming and food production management to produce high-quality livestock products that meet international food safety standard and enough to serve rising domestic and export demands Equally impor-tant is to protect the natural envi-ronment for sustainable develop-mentrdquo

Mr Nguyen Van Trong echoed the needs for technology and modern business solutions for Vietnam aquaculture industry Although fish and shrimp have been growing rapidly both have been facing continuous challenges especially rising production costs lowering prices and disease man-agement

ldquoOur aquaculture industr y might be growing a little too fast especially for catfish farming

ldquoThe industr y must ser i -

ously look into sustainable fa rming management Th i s t ime around the emphasis should be on quality rather than quantity to ensure that growth will continue at sus-tainable pace Pangasius for example we are having market problems so while expanding into new markets we need to be cer tain that Vietnamrsquos products meet safety standards and food hygiene required by impor tersrdquo

Apar t from technology and business solutions on the exhi-bition floors ILDEX Vietnam Aquaculture Conference 2010 under the theme lsquothe new dimen-sions for Vietnam Aquaculture Industryrsquo will be a good educa-tion forum for stakeholders along the production chain from pondhatchery operators to technical level personnel to managers feed manufacturers government officers to grasp the issues facing by the industry and develop suitable policies and aquaculture suppliers supporting the industry

Mo re in fo rMatio n

ILDEX Vietnam 2010 and ILDEX Vietnam Aquaculture Conference 2010Mr Lertwat Chanthatarath Senior Sales Manager Tel +66 2 20342614Email lertwatQsncccomWebsite wwwildexcom

ILDEX Vietnam 2010 the solutions for Vietnam sustainable growth

4 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 5

Aqua News

AE2010 is organised by the European Aquaculture Society and hosted by

the Portuguese Marine Sciences Associate Laboratory CIMAR (CIIMAR Por to and CCMAR Faro)

Portugal and PortoAquaculture production in

Por tugal is modest with some 10000 tonnes of production of which more than half is shell-fish

Marine aquaculture in Portugal is mainly centred on the Algarve coast but more recently land-based production of turbot and other marine species has devel-oped on the Atlantic coast

Currently there is renewed interest in developing aqua-culture in Por tugalrsquos outer-most regions notably in the Azores The Portuguese remain Europersquos highest consumers of fish and shellfish with annual per capita consumption of close to 60kg

Por to is Por tugalrsquos second biggest city and the lsquocapital of the nor thrsquo Its historic centre was declared a World Heritage Site by UNESCO in 1996 and it shared the designation European Culture Capital in 2001The city has excellent flight connections with flag and low cost carriers and road connections with Galicia

and especially with Vigo just 90 minutes away

AE2010 will take place at the Centro de Congressos da Alfacircndega ndash the old customs house on the quay of the Douro River in the heart of Porto and just opposite the famous por t wine cellars that are synonymous with this lively city These will form part of the AE2010 social pro-gramme

An extensive conference programme

The thematic sessions take place each morning of AE2010 and include plenary presentations by invited speakers

They pave the way for the parallel technical sessions of con-tributed presentations in both oral and poster format

A vision for the future status of marine aquaculture in Europe effects of a changing environment on biodiversity and physiology of farmed species Changing envi-ronment is at the heart of the European concerns what will be the impacts at short and medium terms For aquaculture pro-ducing specific species not able to maintain a constant body tem-perature (ectotherms) various influences are expected both for species geographical distribu-tion and so adequacy between species and present rearing sites

and impacts on species physi-ology The European aquaculture in mainly producing molluscs and fishes rapidly developed in several countries in taking into account the interdependences between species physiological needs and local conditions in terms of both more or less sheltered areas and environmental factors changes In a situation of rapid environmental changes what are the measures we have to take to follow them maybe to forecast them and to better prepare the aquacul-ture of tomorrow This review proposes to analyse this new sit-uation Presented by Gilles Boeuf President of the French National Museum of Natural History

A license to produce In the light of the new Commission strategy for the development of European aquaculture what type of gover-nance and regulatory framework is needed What should be the roles of public authorities and NGOrsquos in the development of the sector Presented by Javier Ojeda Apromar Spain

Competing claims ndash joint solu-tions How to integrate aquacul-ture with other coastal activities how can aquaculture contribute to the maritime strategy to coastal zone management and to Natura 2000 objectives Presented by Franccedilois Simard IUCN Global Marine Programme

Consumer demands and con-sumption trends What are the demand and consumption trends and what do European con-sumers prefer How important to consumers is the origin and certification of aquatic products produced lsquolocallyrsquo in Europe or impor ted from third coun-tr ies Presented by Philippe Paquotte DG Mare European Commission

Sustainable sea farming How do we develop sustainable sea farming using the ecosystem approach How is aquaculture linked with natural ecosystem processes and with patrimonial cultivation Presented by Joatildeo Gomes Ferreira New University of Lisbon Portugal

AE2010 is now calling for abstracts for presentation in parallel and poster sessions

These sessions are organised in several groups as follows

bull Hatchery production and spec ies d iver s i f i ca t ion including sessions on

bull Finfish hatcher y produc-tion covering all aspects of juvenile production proc-esses and management

bull Shellfish (juvenile) produc-tion including new develop-ments in breeding hatchery production natural spat col-lection

bull Management of aquaculture

The future of marine aquaculture

Aquaculture Europe 2010 to be held in Porto Portugal from October 5-8 2010 puts the focus on the future of marine aquaculture in Europe

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

stocks including reproduc-tion breeding and genetic improvements

bull Species diversification - finfish including the culture of flatfish tuna and other lsquonewrsquo finfish species for future commercial production

bull Species diversification ndash other species including the culture of octopus cuttlefish abalone crabs seaweeds and other lsquonewrsquo species for future commercial production

bull Production in different envi-ronments and produc-tion technologies including sessions on

bull Land-based production - new production technol-ogies including large-scale RAS production in ar id regions and other land based activities

bull Offshore aquaculture of fish and shellfish

bull Aquaculture on Islands ndash specificities of production in outlying areas in tropical and temperate regions including European outer-most regions

Tools for the development of the sector including sessions on

bull The eco-system approach to aquaculture produc-tion including semi inten-siveextensive coastallagoon aquaculture nutrient man-agement and IMTA invasive species predator control nature management and pat-rimonial conservation

bull Space for aquaculture Carrying capacity and Spatial Planning

bull Aquaculture governance and interactions with fish-eries including policy soci-oeconomic and economic research and interactions commonalities and interde-pendence with fisheries

bull Health quality and well-being including sessions on

bull Human health consumer science and quality including

aquaculture percept ion studies biotoxins quality assessment and detection certification

bull Health management of finfish and shellfish Sponsored by Inter vet Schering-Plough Animal Health

bull Novel feeds ingredients and additives Sponsored by Biomin

bull Aquaculture welfare and ethics

The conference will also include a special Student Workshop and naturally a student reception

Science meets industryAquaculture Europe 2010 will

also feature a special interna-tional trade exhibition organised by the European Aquaculture Society Por tuguese Spanish and International companies will present the latest products and ser vices for aquacul-ture A special farmersrsquo day programme will have an emphasis on aquaculture in Por tugal and in Spain and will focus on topics where the latest research findings can have an impact on the sector

It is hoped that the main Por tuguese and Spanish industr y associations for finfish and shel l fish wil l take advantage of the farmersrsquo day to bring their members to Por to and to use the event for their own meetings

Good local and industry support

AE2010 i s f inanc ia l ly suppor ted by the Area Metropolitana do Porto

The gold sponsor of AE2010 is Sorgal SA

E A S i s p r o u d t o announce the AE2010 Gold Sponsorship of Portuguese a q u a f e e d c o m p a n y Aquasoja Aquasoja is the fish feed brand of Sorgal SA Sorgal SA is par t of a

large Por tuguese agro-indus-trial group Sorgal has produced quality feeds for animal farming for more than 50 years and has always conducted its activities focussing on cost-effectiveness animal welfare and feed safety as top priorities

As par t o f i t s Premier Sponsorship of the European Aquaculture Society Inter vet Schering Plough Animal Health will have a strong presence and will sponsor the health manage-ment session

Feed ingredients and addi-t ives specia l i s t B iomin wi l l sponsor the session on Novel feeds ingredients and addi-tives

Those interested in booking exhibition space andor sponsor-ship opportunities should contact Mario Stael at mariostaelscarletbe

EU to showcase FP6 research

The EU Forum ndash a regular feature of Aquaculture Europe events ndash is currently being planned and will look to showcase the results of two of the biggest integrated research projects carried out by the Commission in aquaculture as well as presenting the view of national research funders The EU Forum will also look forward to the final stages of FP7

Just pr ior to AE2010 the Larvanet network will meet in Porto The latest findings of their research will be presented in a special session within the confer-ence A series of short workshops organised by other EU-funded projects and other relevant ini-tiatives will also take place in the exhibition area and specifically targeted towards exhibitors and trade show visitors

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

Kiotechagil the interna-tional supplier of natural high performance feed

additives to enhance growth health and sustainability in agri-culture and aquaculture will have a strong presence at VIV in The Netherlands in April 2010

The company will be high-lighting their new acidifier pHorce which compliments their range of acid products pHorce will provide a concen-trated acid on a micropearl silica carrier and can be used for decontamination of raw materials as an alternative to

c o r r o s i v e liquids and in finished feed at one kgtonne

In addition Kiotechagil will be showcasing a new broad spectrum anti-mycotoxin product which will combine the benefits of their highly effective alumino-silicate Sorbatox with a primary growth yeast cell wall compo-nent high in both beta glucans and mannan oligosaccharides

Mo re in fo rMatio n

Kiotechagil VIV Stand No C066 in Hall 9Tel +44 1189 813333Email infokiotechagilcom

Kiotechagil to show pHorceful presence at VIV

Animal nutrition company Norel launched a new website wwwnorel

es that will provide up-to-date information about its products and the company activities

Founded in 1980 by Mr Enrique Pablos Perez Norel SA has been steadily growing and developing into a well known brand The original meaning of Norel was the Spanish phrase lsquoNO REconocemos Limitesrsquo l i teral ly meaning lsquoWe donrsquot acknowledge limitsrsquo thus dem-onstrating Norelrsquos philosophy The Skyrsquos The Limit or in other words the possibi l i t ies are endless

Norel has become associated with dynamism and innovation (more than five percent of its turnover is invested in Research and Development) and also with quality and performance thanks to our ISO GMP and FAMI-QS certifications

An important feature of Norel is its focus since the beginning on animal nutrition

Originally Norel was a supplier of raw mater ials for animal nutrition milk replacers whey products and protein extracts In 1982 it star ted the production

of spray fat concentrates as well as organic trace minerals (methionates)

Expansion and develop-ment followed and in 1988 Norel opened i ts b io-technology plant in Leon (Spain)

ldquo We t h e n b e c a m e the fi r st b iotechnology company in Spain spe-cialised in animal nutrition In 1990 we star ted the production of by-pass fats for ruminants Today with our flagship Magnapac we are one of the major pro-ducers worldwide Our devel-opment continued with new factories in Egypt and Mexico and specialized units in Spainrdquo says the company in an inter-view with the press

ldquoAn impor tant date in our history is certainly 2001 when Norel SA merged its activities with Nature SA a company spe-cialized in the production of flavours and sweeteners acidi-fiers antioxidants and mould inhibitors

ldquoWe then became known in the market as lsquoNorel amp Naturersquo at that time it was impor tant for us to keep the name Nature

because our act iv it ies and products were different but at the same time very complemen-tary Today we are pleased to introduce our new logo together with the launch of our brand new website wwwnoreles

The new logo of Norel takes into account the histor y by combin ing the two Nrsquos of Norel and Nature Together they resemble the famous Yin Yang symbol and remind us that the two entities now make one The new logo also represents the infinity symbol a reference to the or ig inal meaning of Norel continue to

grow and the possibilities are endless

I t s new webs i t e comes w i th an improved cor po-rate identity and many new features and updates Most impor tant ly i t has added a news section that wil l keep reader s updated on la test developments and activ it ies of Norel worldwide

Mo re in fo rMatio n

Norel SAJesus Aprendiz 19 1 A y BMadrid NC 28007 SpainTel +34 6698 09659Website wwwnorelnaturecom

NOREL announces new web site and new corporate image

8 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 9

Dishman Group o f Ahmedabad I nd i a a lead ing products

and ser vices provider to the pharmaceutical industr y has announced the appointment of Chris Oates to the position of chief operating officer

Mr Oates will repor t to Nick Green president of the Dishman Globa l Contr act Research and Manufactur ing Ser vices (CRAMS) business

M r O a t e s w i l l a s s u m e responsibil ity for Indian and Chinese operations within the company

He wi l l be managing the development operation and improvement of the systems in the two manufacturing sites in India (Bavla and Naroda) and

the new Shanghai facility which is due for completion mid-2010

In addition to the units in Naroda and China Mr Oates wil l be responsible for the nine operating units at Bavla including a large-scale high potency fac i l i ty which wi l l be inaugurated at the end of January The new state-of-the-ar t high potency facil ity will extend Carbogen Amcisrsquo man-ufactur ing capacity to multi-tonne scale and will be the first of its kind to be located in Asia

M r O a t e s rsquo e x p e r i e n c e combines both product ion and operational roles in the contract manufacturing arena He will play a pivotal role in

expanding the companyrsquos core CRAMS business

Mr Oates holds a bach-elorrsquos degree in applied chem-istr y from the University of Huddersfield in the UK His exper t ise is bui lt on more than 20 years working within the pharmaceutical industr y He most recently ser ved as managing director of Uquifa Mexico His previous expe-r ience includes six year s at Avecia Pharmaceuticals in the UK where he held the position of operations manager and as the position of senior pro-duction manager for Syngenta (Agrochemicals)

ldquo I am looking forward to taking up this new challenge and am very excited by what I

believe will be a very successful future for the companyrdquo said Mr Oates

ldquoDishman can provide the full continuum of ser vices from process RampD to large-scale commercial manufacturing in multiple locations around the globe The company is ideally p l aced to bu i ld upon i t s success to date and become the industr y leading CRAMS par tnerrdquo

Mo re in fo rMatio n

Dishman Europe LimitedSuite 4 De Walden Court 85 New Cavendish Street London W1W 6XDUnited KingdomTel +44 2073 230608 Fax +44 2073 230609Website wwwdishmangroupcom

Dishman appoints Chris Oates as chief operating officerNew role will oversee Dishman Indian and Chinese Facilities

Sponsorship of a keynote session at Aquaculture 2010 confirms Biominrsquos

firm commitment and keen focus on this dynamic growth sector

Biomin embraces the new year with a bold plunge in aqua-culture

Fo r the f i r s t t ime the company wil l be par tner ing the World Aquaculture Society (WAS) with the sponsorship of the Aquafeeds Session at Aquaculture 2010

To be held in San Diego from March 1-5 2010 Aquaculture 2010 is the largest event for the global aquaculture industry Hosted every year by the WAS the global aquaculture event is expected to draw some 4000 participants from over 80 coun-tr ies including high profi le

attendees who form the core of decision-makers and innova-tors in the aquatic business

Biomin is proud to be a session sponsor at the worldrsquos largest aquaculture event Held on March 4 2010 the session on lsquoAquafeedsrsquo will see exper t speakers from com-mercial practice and academia share their i n s i gh t s on ma jor issues in aquafeeds formulation and alter-nat ives to fishmeal and protein replace-ment strategies for a variety of aquatic species

Years of dedicated applied research and investments have strengthened Biominrsquos drive and reputation in providing effective natural-based feed solutions for the aquaculture and aquafeed

business throughout the world A core focus on fundamental applied research forms the basis of Biominrsquos portfolio of science-based aquaculture product solu-tions Biominreg AquaSpecials which includes nutraceuticals probiotics natural growth pro-

moters mycotoxin deactiva-tors and functional feed addi-tives

Dr Pedro Encarnaccedilatildeo Biominrsquos aquacul ture spec ia l i s t says ldquoBiomin is a research-focused and -driven company

ldquoWe have translated our long-

standing competence in animal nutrition for terrestrial species into aquaculture and aquafeed research Our Aquatic Centre for Applied Animals (AQUACAN) in Thailand actively conducts feeding trials for many aquatic species from which new findings

have been translated into innovative and practical solutions in aquafeedsrdquo

Biomin will also par-ticipate as a sponsor at the Australas ian Aquacu l ture 2010

another WAS event to be held in Hobar t Australia in May 2010 and at the Aquaculture Europe that will take place in Por to Por tugal in October 2010 both of which are key events for aquaculture in the coming year

A bold step for BIOMIN aquacultureBiomin proud to be session sponsor at the worldrsquos largest aquaculture event

8 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 9

Aqua News

Tecnoloacutegica de Alimentos SA (TASA) of Peru is the first fishmeal and fish

oil producer to achieve cer ti-fication under the new IFFO Global Standard for Responsible Supply

The cer tification has been awarded to the Callao Nor te plant of TASA the worldrsquos largest fishmeal producer Earlier this week Jorge Mora Operations Director of IFFO presented the

IFFO-Assured certificate to Mario Brescia CEO of TASA at its head-quarters in Lima Peru

IFFO has been developing its RS Standard since early 2008 with the help of a Technical Advisory Committee comprising repre-sentatives from the whole aqua-culture value chain including fish farmers feed producers and retailers and also NGOs

This business-to-business inde-pendently-audited programme will enable IFFO members to demonstrate to their customers that they are offer ing pure marine ingredients manufactured from responsibly sourced and traceable raw materials Whole fish used must come from fish-eries managed under the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

ldquoSince October 2009 when we launched the programme we have had applications from 15 manufacturing companies in four major producing countries sourcing from 10 fisheriesrdquo says IFFO director general Jonathan Shepherd

ldquoWe anticipate that there will be substantial supplies of IFFO-Assured fishmeal and fish oil on the market within months and that up to one third of global

p r o d u c t i o n could be certi-fied by the end of 2010rdquo

ldquoWith th i s p r o g r a m m e IFFO has put in place the first link in a fully certified supply chain for aqua-culture and for land animals such as pigs and poul tr y

It will help the industry demon-strate its commitment to respon-sible manufacture of fishmeal and fish oil as well as to the FAO Code of Conductrdquo said Dr Shepherd

Applicant factories which can demonstrate that they are ldquooper-ating a well managed system that ensures their marine raw mate-rials are responsibly sourced and processed to the IFFO Standardrdquo will be awarded certification

They will be able to offer IFFO-Assured fishmeal and fish oil and have the right to display the RS logo An up-to-date list of cur-rently certified fishmeal plants will be posted on the IFFO web site alongside the full Standard

Characteristics of the IFFO Global Standard for Responsible Supply (RS)

bull Standards set by a multi-stake-holder Technical Advisory

Committee including pro-ducers traders fish farmers feed manufacturers proc-essors retailers standard setting organisations and environmental NGOs The Assessment Management and Certification Process are contracted to Global Trust

bull A programme independ-ently administered by an ISO 651996 accredited certifica-tion body which is a member of the International Accreditation Forum (IFA)

bull I t d e m o n s t r a t e s responsible sourcing o f t r aceable r aw material and respon-sible production to ensure feed safety

bull A business-to-busi-ness tool designed to enable fishmeal and fish oil producers to demonstrate the integr i ty o f the i r products and their processes to their cus-tomers in the value-chain rather than a consumer eco-label

bull Whole fish used must come from fisheries that have been independently assessed and meet the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

bull Exclusion of illegal unre-por ted and unregulated (IUU) fish

bull The factor y must have attained Cer tification to the Internat ional Feed Ingredients Standard (IFIS) of the International Feed Safety Alliance (IFSA) or equiva-lent as proof of responsible manufacturing and the appli-cant must be in possession

of all relevant permits and licenses for the production and sale of fishmeal and fish oil products

bull This programme is com-patible with ISO 651996 (General requirements for bodies operating product cer tification systems) and with EN45011

bull Recogn i ses other cer-t i f i c a t ion p rog r ammes w h i c h h av e d e m o n -

strated equivalency and which are accepted within the industry - eg Marine Stewardship Council (MSC) for the fishery certification element only

The IFFO programme will continue to be developed in 2010 addressing further aspects of responsible supply

Mo re in fo rMatio n

Andrew JacksonTechnical Director IFFO 2 College YardLower Dagnall Street St Albans Hertfordshire AL3 4PAUnited KingdomTel +44 7808 783488 Email ajacksoniffonetWebiste wwwiffonet

First certification awarded under the IFFO Global Standard for Responsible Supply

ldquoThe first link in a fully certified aquaculture supply chain is in placerdquo

ldquoSupplies of ldquoIFFO-Assuredrdquo fishmeal and fish oil will shortly be on the marketrdquo

Jorge Mora (right) Operations Director of IFFO presenting the IFFO-Assured certificate to Mario Brescia (left) CEO of TASA at its headquarters in Lima Peru

10 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 11

Me r c a t o r M e d i a o r g a n i s e r o f t h e Offshore Mariculture

Conference 2010 is pleased to announce that the leading i ndus t r y a s soc i a t ions w i l l be suppor t i n g t he 2010 Conference

The European Aquaculture Techno logy amp I nnova t ion Platform (EATiP) the Federation o f Eu ropean Aquacu l tu re Producers (FEAP) the Global

Aquaculture All iance (GAA) and HGK Grupacija akvakul-ture the aquaculture affi l ia-tion of the Croatian Chamber of Economy (and member of FEAP) have all pledged their suppor t to the Conference that wi l l be held from the

June 16-18 2010 at the Hilton Imper ial Hotel Dubrovnik Croatia

Both EATiP (European Aquaculture Technology amp Innovation Platform) and FEAP (Federation of European Aquaculture Producers) are pleased to suppor t the Offshore Mariculture Conference s ince events such as these give fish farmers t h e o p p o r t u -n i t y t o l e a r n from both indus-t r i a l and RTD

exper ts about relevant technological advances to rece ive in formed updates on key research and policy issues and

to be able to network with col leagues from across the sector says Cour tney Hough general secretary of FEAP and EATiP

As wel l as promoting the event to their members and therefore rais ing awareness of the event and its presti-gious speakers it also means that members of any of the above assoc ia t ions wi l l be en t i t l ed to a 10 pe rcen t d i s count o f f the de lega te rate European Aquaculture Society members will also be ent i t led to the discount as the EAS have continued their suppor t f rom the prev ious even t he ld i n A l i c an te i n 2008

The technical conference will be held on the June 16 and 17 June with a visit to a fish farm on the June 18 2010 The Conference Networking Dinner will take place on the evening of Wednesday June 16 2010 at the fabulous Revelin For tress which was bui lt in 1462 to provide protection to the Eastern City Gate Place Over look ing the Old Ci ty Harbour the Revelins terrace offers what are probably the best views of the Old City and its famous Harbour

Mo re in fo rMatio n

Offshore MaricultureTel +44 1622 820622Website wwwoffshoremariculturecom

Industry support strengthens for 2010 OSM Conference

The full programme will be released shortly sessions will include

bull Policy legal framework and key research updates

bull Practical offshore farming operation

bull Spatial planning and moni-toring

bull Spotlight on Croatiabull Ecological monitoring and

future technologiesbull European and International

case studies

iN THE NEWSYour news could feature in our pages

Do you have an news item that you would like to see in our pages Send your press releases to the Production Manager - Nicky BarnesEmail nickybaquafeedcouk

10 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 11

Aqua News

The aquaculture industry today faces a number of challenges that threaten to restrict its development

and profitability

To live in harmony with consumer expec-tations of the highest possible standards of product quality and to do so in a sustainable and environmentally responsible manner necessitates an innovative coordinated and holistic approach within the aquaculture industry

The requirement for sustainability has focused on the composition of the feed being used as one of the key parameters determining the sustainability of production of a particular species particularly the fish-meal and fish oil content and how it relates to overall feed conversion ratios

Further formulation trends are moving towards low fishmeal and oil content in the grower feeds and finishing feeds designed to restore flesh quality parameters such as omega 3 fats and fillet quality that are con-sidered essential for the promotion of the product and its benefits for human health

This strategy addresses to some extent the limited resources of fishmeal and fish oil

Expectations of sustainabilityThe only way to achieve the expecta-

tions of sustainability and replace or supple-ment fishmeal and fish oil in aquafeeds is to exploit alternative protein and lipid sources

The obvious and traditionally used alter-natives are animal and plant protein sources

However in Europe and now increas-ingly in North America consumers pre-fer aquaculture products to have been

fed without the use of animal by-products due in part to recent health scares in the terrestrial animal sec-tor such as bovine spongiform encepha-lopathy (BSE)

The search is on therefore for not only the simple replacement of protein but the provi-sion of functional nutri-tion addressing both the sustainability and flesh quality requirements of the industry while

meeting the nutritional requirement of the animal being cultured

Alternative vegetable protein sources

Over the last two decades aquaculture research has focused on plant protein sources such as grains pulses and oilseeds as they are generally widely available

However the use of soybean meal and other plant based protein sources such as legume seeds oilseed cakes leaf meals leaf protein concentrates and root tuber meals have been limited due to the presence of a wide variety of anti-nutritional substances in these products and their interaction with the physiology of the animal resulting in depressed growth and feed efficiency mortality and abnormal intestinal morphol-ogy and damage

These substances act in different ways by affecting protein utilisation and digestion (eg protease inhibitors tannins lectins) by affecting mineral utilisation (eg phytate gossypol pigments glucosinolates) antivita-mins and other miscellaneous substances (eg phytoestrogens)

The increasing use of plant protein sources is also raising new concerns about possible contamination by mould derived mycotoxins

This risk is enhanced by the global nature and trading of plant protein sourc-es and the as yet little investigated impact of different mycotoxins and their interac-tions in aquaculture species In 2008 the Rapid Alert System for Food and Feed in

Protein sources Alternative protein sources should also show functionality if they are to play a role in modern aquafeedsby Keith Filer PhD research Manager Asia-Pacific biosciences Centre Alltech inc

Figure 1 A comparison of typical essential amino acid (EAA) requirements for fish and the EAA profile of AquateTM

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

juns

wor

th |

Dre

amst

ime

com

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

040

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08

010

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08

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290

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16 | InternatIonal AquAFeed | March-april 2010

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Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

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20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

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of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

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1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

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Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

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Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

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________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

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E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

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Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

You want to know more about our event Then please visit wwwsummit2010org

Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

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MY

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CMY

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idl10 - Grain Feedai 1222010 45438 PM

40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

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MY

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Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

bull Crevetecbull Danisco Animal Nutritionbull Exquimbull Ge-Pro Aquabull Inve Nutri-Ad

Elevator buckets

Equipment for salebull A Foeth BVbull Condex (UK) Ltd

bull Filter Screen Supply Ltd

Extrudersbull Almex

bull Anderson Internationalbull Baker Perkinsbull Brabender

bull Caliber Controlbull Conforma Clad Incbull Coperionbull ESE Intecbull Fudex Group Spabull Insta Pro International

bull Setrembull Suehiro EPMbull TGC Extrusionbull Wenger

Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

  1. Button 1
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Page 3: March | April 10 - International Aquafeed

aquaI n t E r n A t I o n A l

feedAn internAtionAl mAgAzine for the AquAculture feed industryCONTENTS

PerendalePublishers Ltd International Aquafeed is published six times a year by Perendale Publishers Ltd of the United Kingdom

All data is published in good faith based on information received and while every care is taken to prevent inaccuracies the publishers accept no liability for any errors or omissions or for the consequences of action taken on the basis of information published copyCopyright 2010 Perendale Publishers Ltd All rights reserved No part of this publication may be reproduced in any form or by any means without prior permission of the copyright owner Printed by Perendale Publishers Ltd ISSN 1464-0058

Volume 13 Issue 2 March-April 2010 copy Copyright Perendale Publishers Ltd 2010 All rights reserved

EDITORrsquoS DESK 2

Aqua NewsA bold step for BIOMIN aquaculture 3Strategic Cooperation Agreement between CPF and AKVA 4Perten Instruments appoints managing director in Australia 4ILDEX Vietnam 2010 the solutions for Vietnam sustainable growth 5The future of 6marine aquaculture 6NOREL announces new web site and new corporate image 8Kiotechagil to show pHorceful presence at VIV 8ARCHIVE 8Dishman appoints Chris Oates as chief operating officer 9New role will oversee Dishman Indian and Chinese Facilities 9First certification awarded under the IFFO Global Standard for Responsible Supply 10Industry support strengthens for 2010 OSM Conference 11

ProteinProtein sources - Alternative protein sources should also show 12 functionality if they are to play a role in modern aquafeeds

ProbioticEvaluation of probiotic bacteria in prawn production 18

THE AQUAFEED PHOTOSHOOT 24

ExtrusionProtein damagecomplexation during extrusion 26

VacuumA vacuum batch coating system especially designed for aquafeed and pet food 32

AquafeedsAquafeeds ready to take the plunge 34

Feed ManagementAquafeeds in Tanzania 36Diets for tilapia brood stock Addressing challenges for reducing dependency on fish oil 38

Book Reviews 42

CLASSIFIED ADVERTS 44

IAF WEB LINKS 46

AQUA EVENTS 48

Cov

er im

age

cour

tesy

of

Fusi

on M

arin

e Li

mite

d

EDITORrsquoS DESK W

ELC

OM

E TO

INTE

RN

ATIO

NA

L AQ

UA

FEED

MA

GA

ZIN

E

Croeso (Welcome)

As we leave a very cold dark winter behind us in Northern Europe and the USA we look towards the glimmer of spring and the start a new produc-tion cycle in the Northern Hemisphere Of course this contrasts with the tropics where demand for fish and shellfish production is expanding and

the aquafeed sector must respond accordingly

SE Asia continues to dominate and it is not surprising that International Aquafeed continues to receive invaluable contributions from specialists in this region

I am delighted to overview the contribution of Keith Filer from Alltech Asia ndashPacific concerning the development of novel proteins based on single cell sources such as yeasts and algal blends and how this might contribute significantly to diet formulations for many fish species in future

He focuses on some recent scientific appraisals of products from both a production basis and promotion of health

This fits in with the overall global sustainability agenda and is further elaborated by Daphne Tanrsquos feature which takes a more holistic view of the protein and feed ingredient needs in aquaculture and addresses this topic in an interesting approach from Singapore with an expert eye on the market trends and potential for raw material usage

My editorial associate Professor Krishen Rana from Stirling again addresses feed management issues with his sound knowledge of Africa and this time turns his attention to Tanzania and tilapia and in particular follows with a specialised focus on tilapia brood stock diets

Indeed we must in future include a special feature on the nutritional requirements of brood stock fish in general as the aquafeed industry always needs to produce a range of special feeds for shrimp maturation and large mature fin-fish entering their reproductive stages

As such feed management is an important aspect of good husbandry and I thank Krishen for his excellent contributions to this section and wide applications to effective feed delivery and efficiency gains

The technical issues relating to feed manufacture are highlighted in an article concerning protein damage and stability in the complex extrusion process This is a very important concern and is per-tinent to our understanding of the delicate nature of many feed ingredient and how their nutritional value can be modulated under various conditions We also note the importance of post-extrusion vacuum coating technology for specialised feed additives and oil inclusion

Finally I mention a feature from one of our youngest contributors Tim OrsquoBrine who at 22 is one of my MSc students undertaking his Masters in lsquoSustainable Aquaculture Systemsrsquo

His article reviewing the use of probiotics in prawn production is comprehensive and a useful staging post for further consideration on disease and health management of shrimp

I am sure you will enjoy this spring issue next time I will report directly from the World Aquaculture Society meeting in San Diego and which will no doubt provide many new topics for discussion

March-April 2010

SubSCribEwwwaquafeedcouksubscribephp

International Aquafeed is published six times a year bringing you in-depth features industry news events book reviews and more Subscribers to International Aquafeed also receive a free copy of the International Aquafeed Directory worth UKpound85 For more information please visit our website

2 | InternatIonal AquAFeed | March-april 2010

EditorProfessor Simon DaviesTel +44 1242 267706Email simondaquafeedcouk

Associate EditorProfessor Krishen RanaTel +44 1242 267706Email krishenraquafeedcouk

Editorial Advisory Panelbull Abdel-Fattah M El-Sayed (Egypt)bull Aliro Borquez (Chile)bull Chen Shuping (China)bull Citas Pascual (Philippines)bull Colin Mair (UK)bull Daniel Montero (Spain)bull Dom Bureau (Canada)bull Eric De Muylder (Belgium)bull Miguel A Olvera (Mexico)bull Mike Cremer (USA)bull Ola Flesland (Norway)bull Mohammad R Hasan (Italy)bull Ming DanChen (Thailand)

International Marketing Manager

Caroline WearnTel +44 1242 267706Email carolinewaquafeedcouk

Subscription amp Circulation Manager

Tuti TanTel +44 1242 267706 Email tutitaquafeedcouk

Production Manager

Nicky BarnesTel +44 1242 267706 Email nickybaquafeedcouk

Design amp Page Layout

James TaylorTel +44 1242 267706 Email jamestaquafeedcouk

2 | InternatIonal AquAFeed | March-april 2010

Tel +1 314 739 9191 bull +1 800 288 2726 bull Fax +1 314 739 5880 bull wwwtapcoinccomSt Louis Missouri USA

ELEVATOR BUCKETS - ELEVATOR BOLTS

Jim Cunningham USA

(Speaks English)

ldquoRounded edges =

gentle handlingrdquo

Wherever your product is processed storedor shipped one thing is constantthe

need for high quality buckets thatstand the test of time

Since 1974 Tapco buckets havebeen outperforming all othersin tests and actual usage ndashhandling everything from

feed to fertilizer with care andefficiency Maybe thatrsquos why

Tapco has the two most popular designs in the world Tapco hasa bucket that will interchange and replace existing buckets to giveyou the durability you need to handle abrasive loadshellipwith equal or greater capacity

When you call Tapco yoursquoll be pleased to learnthat your representative literally DOES speakyour language ndash and knows which bucketsare the best choice for your application Visitwwwtapcoinccom to find out moreabout Tapcorsquos world-class features

Thorsten Rogner Europe amp Asia(Speaks German)ldquoCC-XD ndash Toughest on the marketrdquo

Bruce Fagala USA (Speaks English)

ldquoNon-sparking and saferdquo

Jimi Davidson USA (Speaks Spanish)ldquoAvailable in polyethylenepolyurethane and nylonrdquo

FANGED HEADElevator Bolt

FLAT COUNTERSUNKHEAD (NO 1 NORWAY)

Elevator Bolt

Carl Swisher USA amp Canada

(Speaks Spanish)

ldquoXtremely efficientrdquo

Yassine Abbad Middle East amp Africa(Speaks French amp Arabic)ldquoBuckets for every applicationrdquo

John Paul Santangelo Latin America (Speaks Spanish)

ldquoStocking distributors worldwiderdquo

Mike Kerlagon USA (Speaks English)

ldquoSame day shipping availablerdquo

When it Comes to Elevator Bucket PerformanceTapco Speaks Your Language

reg

STYLE SUPER EUROBUCKETtrade CC-XD(XTREME DUTY) amp CC-HD (HEAVY DUTY)

Polyurethane Nylon and Polyethylene Elevator Buckets

The color blue when used in connection with elevator buckets is a US registered trademark owned by Tapco Inc Super EuroBucket trade is a registered trademark of Tapco Inc copy 2010 Tapco Increg All rights reserved

SpeakAd_FINALA4 22310 102 AM Page 1

Strategic Cooperation Agreement between CPF and AKVA

Charoen Phokphand Foods Ltd the worlds leading producer and

suppl ier of farmed White Shrimp and AKVA group ASA the worlds leading supplier of technology for the aquacul-ture industry have signed a stra-tegic cooperation agreement for developing a complete new production system for the pro-duction of white shrimp utilising AKVAs recirculation technology and CPFs broad knowledge in shrimp farming

CPF is working actively to improve the i r product ion methods and technology

A natural next step for the shrimp farming industry is to utilise new technological oppor-tunities to address many of the challenges of the industry and society today

ldquoThis strategic cooperation agreement with AKVA is an important step for us in our strive

towards a transformation of the shrimp farming industry

ldquoBy th i s pro ject we are addressing impor tant issues for the industr y such as the level of which marine resource and land areas are utilised the escaping of shrimp from farms to interfere with wild stocks the control of diseases and efficient battling of parasites the elimi-nation of and risks of pollution from shrimp farms and other possible effects on the habitats where the farming is done Further to this we believe that the technology that this stra-tegic cooperation agreement will produce has the potential also to limit the carbon foot-print of the produced shrimp significantlyrdquo says Dr Chingchai Lohanawatanakul Chairman of the executive Board of CPF

The first step of the strategic cooperation includes developing a full scale prototype of a 200

tonnesyear intensive facility for white shrimp (Litopenaeus Vannamei) as an integrated solution

ldquoFor AKVA group this is an important step forward in our development in the Asian market and the furthering of our recir-culation technology in the global market

ldquoWe appreciate the initia-tives and eagerness that CPF is showing in this project and are looking forward to the results from this cooperation for both par tiesrdquo says Mr Knut Molaug CEO of AKVA group

Mo re in fo rMatio n

Knut MolaugCEOAKVA group ASA Nordlysveien 4PO Box 271 N-4349 BryneNorwayTel +47 901 39522Fax +47 51 778501Email kmolaugakvagroupcom

Back from left Mr Robins P McIntosh (Senior VP CPF) Mr Jan Erik Svensson (Regional Director AKVA group S E Asia) Dr Chingchai Lohawatanakul (Chairman of the Executive Board CPF)Front from left Mr Knut Molaug (CEO AKVA group) Dr Sujint Thammasart (Executive VP CPF)

Je f f R o g e r s h a s b e e n appointed managing director to lead Per ten Instruments

of Australia Pty Ltd He brings over 25 years expe-

rience in the scientific instrument equipment marketplace and is well qualified to lead the company in delivering our world class tech-nology and support

ldquoThis is a very exciting time for

our company We are all looking forward to a great 2010 with our customers and suppliersrdquo says Mr Rogers

He takes over from Rodney B o o t h w h o h a s r e t i r e d a s manag ing d i r ec to r and w i l l now t a ke a Bu s i ne s s Development role within the l a r ge r Per ten In s t r ument s G r o u p R o d n e y f o u n d e d N e w p o r t S c i e n t i f i c a n d l aunched the Rap id V i s co Analyser (RVA) in 1985

ldquoI am looking forward to my new focus on strategic projects a n d r e s e a r c h a n d d e ve l o p -ment of new and applied technol-ogies rdquo says Mr

Booth Mr Booth also serves on the

board of AACC International since September 2009

As par t of the in tegr at ion of Newport Scientific i n t o Pe r t e n I n s t r u m e n t s the legal entity Newport Scientific P t y L t d h a s changed name and is now Perten Instruments of Australia Pty Ltd

The company will continue to provide sales applications and service suppor t for the full range of Swedish and Australian made Per ten products Their focus on local manufactur ing and development of analyt-ical equipment such as the RVA and dough LAB remains unchanged

Mo re in fo rMatio n

Jeff RogersManaging Director Perten Instruments of Australia Pty LtdUnit 1 2 Apollo Street Warriewood NSW 2102 AustraliaTel +61 2 99796992Fax +61 2 99796993Email jrogerspertencomWebsite wwwpertencom

Perten Instruments appoints managing director in Australia

Jeff Rogers Rodney Booth

4 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 5

Aqua News

ILDEX Vietnam 2010 is ready to offer technology and business solutions for Vietnamrsquos live-

stock dairy meat-processing and aquaculture industries according to NEO - the organizer of ILDEX Vietnam 2010 which will take place on March 25-27 2010 at the New Saigon Exhibition and Convention Center Ho Chi Minh City Vietnam

NCC Exhibition Organizer Co Ltd (NEO) announced the read-iness of ILDEX Vietnam 2010 which will take place on March 25-27 2010 at the New Saigon Exhibit ion and Convention Center in Ho Chi Minh City Vietnam

At the Kick-off Launching of ILDEX Vietnam 2010 Ms Ladda Mongkolchaivivat general manager NCC Exh ib i t ion Organizer (NEO) told industry representative that ldquoILDEX Vietnam 2010 will be the largest livestock exhibition in Vietnam It will be a technology showcase of 125 companies from 22 coun-tries including country pavilion from France Singapore Korea China Shanghai City and the Netherlands

ldquoA number of international governmental and industr ial bodies such as Korean Animal Health Products Association Canadian Swine Association

British Pig Association Vietnam Depar tment of Animal Health V i e t n am Depa r tmen t o f Livestock Production and CCPIT Shanghai have confirmed their par ticipation at this industrial eventrdquo

Wi t h heav y p romot i on campaign and full government suppor t especially from the Ministry of Agricultural and Rural Development of Vietnam NEO believes that ILDEX Vietnam 2010 will attract a lot of traders and buyers from over 46 cities and provinces surrounding Ho Chi Minh City Free shuttle service will be provided for trade visitors from those areas

ILDEX Vietnam 2010 comes in perfect timing as Vietnam live-stock dair y meat-processing and aquaculture industries are searching for new technology and business solutions to move the industry to the next level

Aiming to provide the industry representative with clearer perspective of Vietnam live-stock and aquaculture indus-tries ILDEX invited Professor Hoang Kim Giao director of depar tment of livestock pro-duction Ministry of Agriculture and Rural Development and Mr Nguyen Van Trong vice director-general Aquaculture Research Institute II of Vietnam to give

keynote speeches on ldquoHusbandry in Vietnam ndash Current Status and Development Goalsrdquo and ldquoOver view Aquaculture in Vietnamrdquo respectively

Professor Hoang Kim Giao under lined the impor tant of Vietnam livestock industry

However he said ldquoIt is time for Vietnam to change the struc-ture of its agriculture and rural development Priority should be given to development of live-stock industry including industri-alization of livestock farming and food production management to produce high-quality livestock products that meet international food safety standard and enough to serve rising domestic and export demands Equally impor-tant is to protect the natural envi-ronment for sustainable develop-mentrdquo

Mr Nguyen Van Trong echoed the needs for technology and modern business solutions for Vietnam aquaculture industry Although fish and shrimp have been growing rapidly both have been facing continuous challenges especially rising production costs lowering prices and disease man-agement

ldquoOur aquaculture industr y might be growing a little too fast especially for catfish farming

ldquoThe industr y must ser i -

ously look into sustainable fa rming management Th i s t ime around the emphasis should be on quality rather than quantity to ensure that growth will continue at sus-tainable pace Pangasius for example we are having market problems so while expanding into new markets we need to be cer tain that Vietnamrsquos products meet safety standards and food hygiene required by impor tersrdquo

Apar t from technology and business solutions on the exhi-bition floors ILDEX Vietnam Aquaculture Conference 2010 under the theme lsquothe new dimen-sions for Vietnam Aquaculture Industryrsquo will be a good educa-tion forum for stakeholders along the production chain from pondhatchery operators to technical level personnel to managers feed manufacturers government officers to grasp the issues facing by the industry and develop suitable policies and aquaculture suppliers supporting the industry

Mo re in fo rMatio n

ILDEX Vietnam 2010 and ILDEX Vietnam Aquaculture Conference 2010Mr Lertwat Chanthatarath Senior Sales Manager Tel +66 2 20342614Email lertwatQsncccomWebsite wwwildexcom

ILDEX Vietnam 2010 the solutions for Vietnam sustainable growth

4 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 5

Aqua News

AE2010 is organised by the European Aquaculture Society and hosted by

the Portuguese Marine Sciences Associate Laboratory CIMAR (CIIMAR Por to and CCMAR Faro)

Portugal and PortoAquaculture production in

Por tugal is modest with some 10000 tonnes of production of which more than half is shell-fish

Marine aquaculture in Portugal is mainly centred on the Algarve coast but more recently land-based production of turbot and other marine species has devel-oped on the Atlantic coast

Currently there is renewed interest in developing aqua-culture in Por tugalrsquos outer-most regions notably in the Azores The Portuguese remain Europersquos highest consumers of fish and shellfish with annual per capita consumption of close to 60kg

Por to is Por tugalrsquos second biggest city and the lsquocapital of the nor thrsquo Its historic centre was declared a World Heritage Site by UNESCO in 1996 and it shared the designation European Culture Capital in 2001The city has excellent flight connections with flag and low cost carriers and road connections with Galicia

and especially with Vigo just 90 minutes away

AE2010 will take place at the Centro de Congressos da Alfacircndega ndash the old customs house on the quay of the Douro River in the heart of Porto and just opposite the famous por t wine cellars that are synonymous with this lively city These will form part of the AE2010 social pro-gramme

An extensive conference programme

The thematic sessions take place each morning of AE2010 and include plenary presentations by invited speakers

They pave the way for the parallel technical sessions of con-tributed presentations in both oral and poster format

A vision for the future status of marine aquaculture in Europe effects of a changing environment on biodiversity and physiology of farmed species Changing envi-ronment is at the heart of the European concerns what will be the impacts at short and medium terms For aquaculture pro-ducing specific species not able to maintain a constant body tem-perature (ectotherms) various influences are expected both for species geographical distribu-tion and so adequacy between species and present rearing sites

and impacts on species physi-ology The European aquaculture in mainly producing molluscs and fishes rapidly developed in several countries in taking into account the interdependences between species physiological needs and local conditions in terms of both more or less sheltered areas and environmental factors changes In a situation of rapid environmental changes what are the measures we have to take to follow them maybe to forecast them and to better prepare the aquacul-ture of tomorrow This review proposes to analyse this new sit-uation Presented by Gilles Boeuf President of the French National Museum of Natural History

A license to produce In the light of the new Commission strategy for the development of European aquaculture what type of gover-nance and regulatory framework is needed What should be the roles of public authorities and NGOrsquos in the development of the sector Presented by Javier Ojeda Apromar Spain

Competing claims ndash joint solu-tions How to integrate aquacul-ture with other coastal activities how can aquaculture contribute to the maritime strategy to coastal zone management and to Natura 2000 objectives Presented by Franccedilois Simard IUCN Global Marine Programme

Consumer demands and con-sumption trends What are the demand and consumption trends and what do European con-sumers prefer How important to consumers is the origin and certification of aquatic products produced lsquolocallyrsquo in Europe or impor ted from third coun-tr ies Presented by Philippe Paquotte DG Mare European Commission

Sustainable sea farming How do we develop sustainable sea farming using the ecosystem approach How is aquaculture linked with natural ecosystem processes and with patrimonial cultivation Presented by Joatildeo Gomes Ferreira New University of Lisbon Portugal

AE2010 is now calling for abstracts for presentation in parallel and poster sessions

These sessions are organised in several groups as follows

bull Hatchery production and spec ies d iver s i f i ca t ion including sessions on

bull Finfish hatcher y produc-tion covering all aspects of juvenile production proc-esses and management

bull Shellfish (juvenile) produc-tion including new develop-ments in breeding hatchery production natural spat col-lection

bull Management of aquaculture

The future of marine aquaculture

Aquaculture Europe 2010 to be held in Porto Portugal from October 5-8 2010 puts the focus on the future of marine aquaculture in Europe

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

stocks including reproduc-tion breeding and genetic improvements

bull Species diversification - finfish including the culture of flatfish tuna and other lsquonewrsquo finfish species for future commercial production

bull Species diversification ndash other species including the culture of octopus cuttlefish abalone crabs seaweeds and other lsquonewrsquo species for future commercial production

bull Production in different envi-ronments and produc-tion technologies including sessions on

bull Land-based production - new production technol-ogies including large-scale RAS production in ar id regions and other land based activities

bull Offshore aquaculture of fish and shellfish

bull Aquaculture on Islands ndash specificities of production in outlying areas in tropical and temperate regions including European outer-most regions

Tools for the development of the sector including sessions on

bull The eco-system approach to aquaculture produc-tion including semi inten-siveextensive coastallagoon aquaculture nutrient man-agement and IMTA invasive species predator control nature management and pat-rimonial conservation

bull Space for aquaculture Carrying capacity and Spatial Planning

bull Aquaculture governance and interactions with fish-eries including policy soci-oeconomic and economic research and interactions commonalities and interde-pendence with fisheries

bull Health quality and well-being including sessions on

bull Human health consumer science and quality including

aquaculture percept ion studies biotoxins quality assessment and detection certification

bull Health management of finfish and shellfish Sponsored by Inter vet Schering-Plough Animal Health

bull Novel feeds ingredients and additives Sponsored by Biomin

bull Aquaculture welfare and ethics

The conference will also include a special Student Workshop and naturally a student reception

Science meets industryAquaculture Europe 2010 will

also feature a special interna-tional trade exhibition organised by the European Aquaculture Society Por tuguese Spanish and International companies will present the latest products and ser vices for aquacul-ture A special farmersrsquo day programme will have an emphasis on aquaculture in Por tugal and in Spain and will focus on topics where the latest research findings can have an impact on the sector

It is hoped that the main Por tuguese and Spanish industr y associations for finfish and shel l fish wil l take advantage of the farmersrsquo day to bring their members to Por to and to use the event for their own meetings

Good local and industry support

AE2010 i s f inanc ia l ly suppor ted by the Area Metropolitana do Porto

The gold sponsor of AE2010 is Sorgal SA

E A S i s p r o u d t o announce the AE2010 Gold Sponsorship of Portuguese a q u a f e e d c o m p a n y Aquasoja Aquasoja is the fish feed brand of Sorgal SA Sorgal SA is par t of a

large Por tuguese agro-indus-trial group Sorgal has produced quality feeds for animal farming for more than 50 years and has always conducted its activities focussing on cost-effectiveness animal welfare and feed safety as top priorities

As par t o f i t s Premier Sponsorship of the European Aquaculture Society Inter vet Schering Plough Animal Health will have a strong presence and will sponsor the health manage-ment session

Feed ingredients and addi-t ives specia l i s t B iomin wi l l sponsor the session on Novel feeds ingredients and addi-tives

Those interested in booking exhibition space andor sponsor-ship opportunities should contact Mario Stael at mariostaelscarletbe

EU to showcase FP6 research

The EU Forum ndash a regular feature of Aquaculture Europe events ndash is currently being planned and will look to showcase the results of two of the biggest integrated research projects carried out by the Commission in aquaculture as well as presenting the view of national research funders The EU Forum will also look forward to the final stages of FP7

Just pr ior to AE2010 the Larvanet network will meet in Porto The latest findings of their research will be presented in a special session within the confer-ence A series of short workshops organised by other EU-funded projects and other relevant ini-tiatives will also take place in the exhibition area and specifically targeted towards exhibitors and trade show visitors

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

Kiotechagil the interna-tional supplier of natural high performance feed

additives to enhance growth health and sustainability in agri-culture and aquaculture will have a strong presence at VIV in The Netherlands in April 2010

The company will be high-lighting their new acidifier pHorce which compliments their range of acid products pHorce will provide a concen-trated acid on a micropearl silica carrier and can be used for decontamination of raw materials as an alternative to

c o r r o s i v e liquids and in finished feed at one kgtonne

In addition Kiotechagil will be showcasing a new broad spectrum anti-mycotoxin product which will combine the benefits of their highly effective alumino-silicate Sorbatox with a primary growth yeast cell wall compo-nent high in both beta glucans and mannan oligosaccharides

Mo re in fo rMatio n

Kiotechagil VIV Stand No C066 in Hall 9Tel +44 1189 813333Email infokiotechagilcom

Kiotechagil to show pHorceful presence at VIV

Animal nutrition company Norel launched a new website wwwnorel

es that will provide up-to-date information about its products and the company activities

Founded in 1980 by Mr Enrique Pablos Perez Norel SA has been steadily growing and developing into a well known brand The original meaning of Norel was the Spanish phrase lsquoNO REconocemos Limitesrsquo l i teral ly meaning lsquoWe donrsquot acknowledge limitsrsquo thus dem-onstrating Norelrsquos philosophy The Skyrsquos The Limit or in other words the possibi l i t ies are endless

Norel has become associated with dynamism and innovation (more than five percent of its turnover is invested in Research and Development) and also with quality and performance thanks to our ISO GMP and FAMI-QS certifications

An important feature of Norel is its focus since the beginning on animal nutrition

Originally Norel was a supplier of raw mater ials for animal nutrition milk replacers whey products and protein extracts In 1982 it star ted the production

of spray fat concentrates as well as organic trace minerals (methionates)

Expansion and develop-ment followed and in 1988 Norel opened i ts b io-technology plant in Leon (Spain)

ldquo We t h e n b e c a m e the fi r st b iotechnology company in Spain spe-cialised in animal nutrition In 1990 we star ted the production of by-pass fats for ruminants Today with our flagship Magnapac we are one of the major pro-ducers worldwide Our devel-opment continued with new factories in Egypt and Mexico and specialized units in Spainrdquo says the company in an inter-view with the press

ldquoAn impor tant date in our history is certainly 2001 when Norel SA merged its activities with Nature SA a company spe-cialized in the production of flavours and sweeteners acidi-fiers antioxidants and mould inhibitors

ldquoWe then became known in the market as lsquoNorel amp Naturersquo at that time it was impor tant for us to keep the name Nature

because our act iv it ies and products were different but at the same time very complemen-tary Today we are pleased to introduce our new logo together with the launch of our brand new website wwwnoreles

The new logo of Norel takes into account the histor y by combin ing the two Nrsquos of Norel and Nature Together they resemble the famous Yin Yang symbol and remind us that the two entities now make one The new logo also represents the infinity symbol a reference to the or ig inal meaning of Norel continue to

grow and the possibilities are endless

I t s new webs i t e comes w i th an improved cor po-rate identity and many new features and updates Most impor tant ly i t has added a news section that wil l keep reader s updated on la test developments and activ it ies of Norel worldwide

Mo re in fo rMatio n

Norel SAJesus Aprendiz 19 1 A y BMadrid NC 28007 SpainTel +34 6698 09659Website wwwnorelnaturecom

NOREL announces new web site and new corporate image

8 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 9

Dishman Group o f Ahmedabad I nd i a a lead ing products

and ser vices provider to the pharmaceutical industr y has announced the appointment of Chris Oates to the position of chief operating officer

Mr Oates will repor t to Nick Green president of the Dishman Globa l Contr act Research and Manufactur ing Ser vices (CRAMS) business

M r O a t e s w i l l a s s u m e responsibil ity for Indian and Chinese operations within the company

He wi l l be managing the development operation and improvement of the systems in the two manufacturing sites in India (Bavla and Naroda) and

the new Shanghai facility which is due for completion mid-2010

In addition to the units in Naroda and China Mr Oates wil l be responsible for the nine operating units at Bavla including a large-scale high potency fac i l i ty which wi l l be inaugurated at the end of January The new state-of-the-ar t high potency facil ity will extend Carbogen Amcisrsquo man-ufactur ing capacity to multi-tonne scale and will be the first of its kind to be located in Asia

M r O a t e s rsquo e x p e r i e n c e combines both product ion and operational roles in the contract manufacturing arena He will play a pivotal role in

expanding the companyrsquos core CRAMS business

Mr Oates holds a bach-elorrsquos degree in applied chem-istr y from the University of Huddersfield in the UK His exper t ise is bui lt on more than 20 years working within the pharmaceutical industr y He most recently ser ved as managing director of Uquifa Mexico His previous expe-r ience includes six year s at Avecia Pharmaceuticals in the UK where he held the position of operations manager and as the position of senior pro-duction manager for Syngenta (Agrochemicals)

ldquo I am looking forward to taking up this new challenge and am very excited by what I

believe will be a very successful future for the companyrdquo said Mr Oates

ldquoDishman can provide the full continuum of ser vices from process RampD to large-scale commercial manufacturing in multiple locations around the globe The company is ideally p l aced to bu i ld upon i t s success to date and become the industr y leading CRAMS par tnerrdquo

Mo re in fo rMatio n

Dishman Europe LimitedSuite 4 De Walden Court 85 New Cavendish Street London W1W 6XDUnited KingdomTel +44 2073 230608 Fax +44 2073 230609Website wwwdishmangroupcom

Dishman appoints Chris Oates as chief operating officerNew role will oversee Dishman Indian and Chinese Facilities

Sponsorship of a keynote session at Aquaculture 2010 confirms Biominrsquos

firm commitment and keen focus on this dynamic growth sector

Biomin embraces the new year with a bold plunge in aqua-culture

Fo r the f i r s t t ime the company wil l be par tner ing the World Aquaculture Society (WAS) with the sponsorship of the Aquafeeds Session at Aquaculture 2010

To be held in San Diego from March 1-5 2010 Aquaculture 2010 is the largest event for the global aquaculture industry Hosted every year by the WAS the global aquaculture event is expected to draw some 4000 participants from over 80 coun-tr ies including high profi le

attendees who form the core of decision-makers and innova-tors in the aquatic business

Biomin is proud to be a session sponsor at the worldrsquos largest aquaculture event Held on March 4 2010 the session on lsquoAquafeedsrsquo will see exper t speakers from com-mercial practice and academia share their i n s i gh t s on ma jor issues in aquafeeds formulation and alter-nat ives to fishmeal and protein replace-ment strategies for a variety of aquatic species

Years of dedicated applied research and investments have strengthened Biominrsquos drive and reputation in providing effective natural-based feed solutions for the aquaculture and aquafeed

business throughout the world A core focus on fundamental applied research forms the basis of Biominrsquos portfolio of science-based aquaculture product solu-tions Biominreg AquaSpecials which includes nutraceuticals probiotics natural growth pro-

moters mycotoxin deactiva-tors and functional feed addi-tives

Dr Pedro Encarnaccedilatildeo Biominrsquos aquacul ture spec ia l i s t says ldquoBiomin is a research-focused and -driven company

ldquoWe have translated our long-

standing competence in animal nutrition for terrestrial species into aquaculture and aquafeed research Our Aquatic Centre for Applied Animals (AQUACAN) in Thailand actively conducts feeding trials for many aquatic species from which new findings

have been translated into innovative and practical solutions in aquafeedsrdquo

Biomin will also par-ticipate as a sponsor at the Australas ian Aquacu l ture 2010

another WAS event to be held in Hobar t Australia in May 2010 and at the Aquaculture Europe that will take place in Por to Por tugal in October 2010 both of which are key events for aquaculture in the coming year

A bold step for BIOMIN aquacultureBiomin proud to be session sponsor at the worldrsquos largest aquaculture event

8 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 9

Aqua News

Tecnoloacutegica de Alimentos SA (TASA) of Peru is the first fishmeal and fish

oil producer to achieve cer ti-fication under the new IFFO Global Standard for Responsible Supply

The cer tification has been awarded to the Callao Nor te plant of TASA the worldrsquos largest fishmeal producer Earlier this week Jorge Mora Operations Director of IFFO presented the

IFFO-Assured certificate to Mario Brescia CEO of TASA at its head-quarters in Lima Peru

IFFO has been developing its RS Standard since early 2008 with the help of a Technical Advisory Committee comprising repre-sentatives from the whole aqua-culture value chain including fish farmers feed producers and retailers and also NGOs

This business-to-business inde-pendently-audited programme will enable IFFO members to demonstrate to their customers that they are offer ing pure marine ingredients manufactured from responsibly sourced and traceable raw materials Whole fish used must come from fish-eries managed under the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

ldquoSince October 2009 when we launched the programme we have had applications from 15 manufacturing companies in four major producing countries sourcing from 10 fisheriesrdquo says IFFO director general Jonathan Shepherd

ldquoWe anticipate that there will be substantial supplies of IFFO-Assured fishmeal and fish oil on the market within months and that up to one third of global

p r o d u c t i o n could be certi-fied by the end of 2010rdquo

ldquoWith th i s p r o g r a m m e IFFO has put in place the first link in a fully certified supply chain for aqua-culture and for land animals such as pigs and poul tr y

It will help the industry demon-strate its commitment to respon-sible manufacture of fishmeal and fish oil as well as to the FAO Code of Conductrdquo said Dr Shepherd

Applicant factories which can demonstrate that they are ldquooper-ating a well managed system that ensures their marine raw mate-rials are responsibly sourced and processed to the IFFO Standardrdquo will be awarded certification

They will be able to offer IFFO-Assured fishmeal and fish oil and have the right to display the RS logo An up-to-date list of cur-rently certified fishmeal plants will be posted on the IFFO web site alongside the full Standard

Characteristics of the IFFO Global Standard for Responsible Supply (RS)

bull Standards set by a multi-stake-holder Technical Advisory

Committee including pro-ducers traders fish farmers feed manufacturers proc-essors retailers standard setting organisations and environmental NGOs The Assessment Management and Certification Process are contracted to Global Trust

bull A programme independ-ently administered by an ISO 651996 accredited certifica-tion body which is a member of the International Accreditation Forum (IFA)

bull I t d e m o n s t r a t e s responsible sourcing o f t r aceable r aw material and respon-sible production to ensure feed safety

bull A business-to-busi-ness tool designed to enable fishmeal and fish oil producers to demonstrate the integr i ty o f the i r products and their processes to their cus-tomers in the value-chain rather than a consumer eco-label

bull Whole fish used must come from fisheries that have been independently assessed and meet the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

bull Exclusion of illegal unre-por ted and unregulated (IUU) fish

bull The factor y must have attained Cer tification to the Internat ional Feed Ingredients Standard (IFIS) of the International Feed Safety Alliance (IFSA) or equiva-lent as proof of responsible manufacturing and the appli-cant must be in possession

of all relevant permits and licenses for the production and sale of fishmeal and fish oil products

bull This programme is com-patible with ISO 651996 (General requirements for bodies operating product cer tification systems) and with EN45011

bull Recogn i ses other cer-t i f i c a t ion p rog r ammes w h i c h h av e d e m o n -

strated equivalency and which are accepted within the industry - eg Marine Stewardship Council (MSC) for the fishery certification element only

The IFFO programme will continue to be developed in 2010 addressing further aspects of responsible supply

Mo re in fo rMatio n

Andrew JacksonTechnical Director IFFO 2 College YardLower Dagnall Street St Albans Hertfordshire AL3 4PAUnited KingdomTel +44 7808 783488 Email ajacksoniffonetWebiste wwwiffonet

First certification awarded under the IFFO Global Standard for Responsible Supply

ldquoThe first link in a fully certified aquaculture supply chain is in placerdquo

ldquoSupplies of ldquoIFFO-Assuredrdquo fishmeal and fish oil will shortly be on the marketrdquo

Jorge Mora (right) Operations Director of IFFO presenting the IFFO-Assured certificate to Mario Brescia (left) CEO of TASA at its headquarters in Lima Peru

10 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 11

Me r c a t o r M e d i a o r g a n i s e r o f t h e Offshore Mariculture

Conference 2010 is pleased to announce that the leading i ndus t r y a s soc i a t ions w i l l be suppor t i n g t he 2010 Conference

The European Aquaculture Techno logy amp I nnova t ion Platform (EATiP) the Federation o f Eu ropean Aquacu l tu re Producers (FEAP) the Global

Aquaculture All iance (GAA) and HGK Grupacija akvakul-ture the aquaculture affi l ia-tion of the Croatian Chamber of Economy (and member of FEAP) have all pledged their suppor t to the Conference that wi l l be held from the

June 16-18 2010 at the Hilton Imper ial Hotel Dubrovnik Croatia

Both EATiP (European Aquaculture Technology amp Innovation Platform) and FEAP (Federation of European Aquaculture Producers) are pleased to suppor t the Offshore Mariculture Conference s ince events such as these give fish farmers t h e o p p o r t u -n i t y t o l e a r n from both indus-t r i a l and RTD

exper ts about relevant technological advances to rece ive in formed updates on key research and policy issues and

to be able to network with col leagues from across the sector says Cour tney Hough general secretary of FEAP and EATiP

As wel l as promoting the event to their members and therefore rais ing awareness of the event and its presti-gious speakers it also means that members of any of the above assoc ia t ions wi l l be en t i t l ed to a 10 pe rcen t d i s count o f f the de lega te rate European Aquaculture Society members will also be ent i t led to the discount as the EAS have continued their suppor t f rom the prev ious even t he ld i n A l i c an te i n 2008

The technical conference will be held on the June 16 and 17 June with a visit to a fish farm on the June 18 2010 The Conference Networking Dinner will take place on the evening of Wednesday June 16 2010 at the fabulous Revelin For tress which was bui lt in 1462 to provide protection to the Eastern City Gate Place Over look ing the Old Ci ty Harbour the Revelins terrace offers what are probably the best views of the Old City and its famous Harbour

Mo re in fo rMatio n

Offshore MaricultureTel +44 1622 820622Website wwwoffshoremariculturecom

Industry support strengthens for 2010 OSM Conference

The full programme will be released shortly sessions will include

bull Policy legal framework and key research updates

bull Practical offshore farming operation

bull Spatial planning and moni-toring

bull Spotlight on Croatiabull Ecological monitoring and

future technologiesbull European and International

case studies

iN THE NEWSYour news could feature in our pages

Do you have an news item that you would like to see in our pages Send your press releases to the Production Manager - Nicky BarnesEmail nickybaquafeedcouk

10 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 11

Aqua News

The aquaculture industry today faces a number of challenges that threaten to restrict its development

and profitability

To live in harmony with consumer expec-tations of the highest possible standards of product quality and to do so in a sustainable and environmentally responsible manner necessitates an innovative coordinated and holistic approach within the aquaculture industry

The requirement for sustainability has focused on the composition of the feed being used as one of the key parameters determining the sustainability of production of a particular species particularly the fish-meal and fish oil content and how it relates to overall feed conversion ratios

Further formulation trends are moving towards low fishmeal and oil content in the grower feeds and finishing feeds designed to restore flesh quality parameters such as omega 3 fats and fillet quality that are con-sidered essential for the promotion of the product and its benefits for human health

This strategy addresses to some extent the limited resources of fishmeal and fish oil

Expectations of sustainabilityThe only way to achieve the expecta-

tions of sustainability and replace or supple-ment fishmeal and fish oil in aquafeeds is to exploit alternative protein and lipid sources

The obvious and traditionally used alter-natives are animal and plant protein sources

However in Europe and now increas-ingly in North America consumers pre-fer aquaculture products to have been

fed without the use of animal by-products due in part to recent health scares in the terrestrial animal sec-tor such as bovine spongiform encepha-lopathy (BSE)

The search is on therefore for not only the simple replacement of protein but the provi-sion of functional nutri-tion addressing both the sustainability and flesh quality requirements of the industry while

meeting the nutritional requirement of the animal being cultured

Alternative vegetable protein sources

Over the last two decades aquaculture research has focused on plant protein sources such as grains pulses and oilseeds as they are generally widely available

However the use of soybean meal and other plant based protein sources such as legume seeds oilseed cakes leaf meals leaf protein concentrates and root tuber meals have been limited due to the presence of a wide variety of anti-nutritional substances in these products and their interaction with the physiology of the animal resulting in depressed growth and feed efficiency mortality and abnormal intestinal morphol-ogy and damage

These substances act in different ways by affecting protein utilisation and digestion (eg protease inhibitors tannins lectins) by affecting mineral utilisation (eg phytate gossypol pigments glucosinolates) antivita-mins and other miscellaneous substances (eg phytoestrogens)

The increasing use of plant protein sources is also raising new concerns about possible contamination by mould derived mycotoxins

This risk is enhanced by the global nature and trading of plant protein sourc-es and the as yet little investigated impact of different mycotoxins and their interac-tions in aquaculture species In 2008 the Rapid Alert System for Food and Feed in

Protein sources Alternative protein sources should also show functionality if they are to play a role in modern aquafeedsby Keith Filer PhD research Manager Asia-Pacific biosciences Centre Alltech inc

Figure 1 A comparison of typical essential amino acid (EAA) requirements for fish and the EAA profile of AquateTM

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

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the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

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12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

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retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

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16 | InternatIonal AquAFeed | March-april 2010

Alltechreg has developed a range of products to address dietary requirements at different

stages of life in aquaculture species These sustainable product solutions are proven to

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Give your fish and shrimp the Alltech Aqua Advantage Contact your Alltech representative today for more information

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Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

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of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

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23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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IAF1001

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(UKpound15 per copy)

Subscribe to IAFSubscribers to IAF receive 5 issues

per year plus a copy of theInternational Aquafeed Directory amp

Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

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Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

Tel Fax

Mobile

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

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Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

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Address

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Mobile

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inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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To place a classified advert in our next issue and take

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WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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Alltech Inc - wwwalltechcom

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Andritz Feed amp Biofuel - wwwandritzcom

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

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BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 4: March | April 10 - International Aquafeed

EDITORrsquoS DESK W

ELC

OM

E TO

INTE

RN

ATIO

NA

L AQ

UA

FEED

MA

GA

ZIN

E

Croeso (Welcome)

As we leave a very cold dark winter behind us in Northern Europe and the USA we look towards the glimmer of spring and the start a new produc-tion cycle in the Northern Hemisphere Of course this contrasts with the tropics where demand for fish and shellfish production is expanding and

the aquafeed sector must respond accordingly

SE Asia continues to dominate and it is not surprising that International Aquafeed continues to receive invaluable contributions from specialists in this region

I am delighted to overview the contribution of Keith Filer from Alltech Asia ndashPacific concerning the development of novel proteins based on single cell sources such as yeasts and algal blends and how this might contribute significantly to diet formulations for many fish species in future

He focuses on some recent scientific appraisals of products from both a production basis and promotion of health

This fits in with the overall global sustainability agenda and is further elaborated by Daphne Tanrsquos feature which takes a more holistic view of the protein and feed ingredient needs in aquaculture and addresses this topic in an interesting approach from Singapore with an expert eye on the market trends and potential for raw material usage

My editorial associate Professor Krishen Rana from Stirling again addresses feed management issues with his sound knowledge of Africa and this time turns his attention to Tanzania and tilapia and in particular follows with a specialised focus on tilapia brood stock diets

Indeed we must in future include a special feature on the nutritional requirements of brood stock fish in general as the aquafeed industry always needs to produce a range of special feeds for shrimp maturation and large mature fin-fish entering their reproductive stages

As such feed management is an important aspect of good husbandry and I thank Krishen for his excellent contributions to this section and wide applications to effective feed delivery and efficiency gains

The technical issues relating to feed manufacture are highlighted in an article concerning protein damage and stability in the complex extrusion process This is a very important concern and is per-tinent to our understanding of the delicate nature of many feed ingredient and how their nutritional value can be modulated under various conditions We also note the importance of post-extrusion vacuum coating technology for specialised feed additives and oil inclusion

Finally I mention a feature from one of our youngest contributors Tim OrsquoBrine who at 22 is one of my MSc students undertaking his Masters in lsquoSustainable Aquaculture Systemsrsquo

His article reviewing the use of probiotics in prawn production is comprehensive and a useful staging post for further consideration on disease and health management of shrimp

I am sure you will enjoy this spring issue next time I will report directly from the World Aquaculture Society meeting in San Diego and which will no doubt provide many new topics for discussion

March-April 2010

SubSCribEwwwaquafeedcouksubscribephp

International Aquafeed is published six times a year bringing you in-depth features industry news events book reviews and more Subscribers to International Aquafeed also receive a free copy of the International Aquafeed Directory worth UKpound85 For more information please visit our website

2 | InternatIonal AquAFeed | March-april 2010

EditorProfessor Simon DaviesTel +44 1242 267706Email simondaquafeedcouk

Associate EditorProfessor Krishen RanaTel +44 1242 267706Email krishenraquafeedcouk

Editorial Advisory Panelbull Abdel-Fattah M El-Sayed (Egypt)bull Aliro Borquez (Chile)bull Chen Shuping (China)bull Citas Pascual (Philippines)bull Colin Mair (UK)bull Daniel Montero (Spain)bull Dom Bureau (Canada)bull Eric De Muylder (Belgium)bull Miguel A Olvera (Mexico)bull Mike Cremer (USA)bull Ola Flesland (Norway)bull Mohammad R Hasan (Italy)bull Ming DanChen (Thailand)

International Marketing Manager

Caroline WearnTel +44 1242 267706Email carolinewaquafeedcouk

Subscription amp Circulation Manager

Tuti TanTel +44 1242 267706 Email tutitaquafeedcouk

Production Manager

Nicky BarnesTel +44 1242 267706 Email nickybaquafeedcouk

Design amp Page Layout

James TaylorTel +44 1242 267706 Email jamestaquafeedcouk

2 | InternatIonal AquAFeed | March-april 2010

Tel +1 314 739 9191 bull +1 800 288 2726 bull Fax +1 314 739 5880 bull wwwtapcoinccomSt Louis Missouri USA

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Wherever your product is processed storedor shipped one thing is constantthe

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Since 1974 Tapco buckets havebeen outperforming all othersin tests and actual usage ndashhandling everything from

feed to fertilizer with care andefficiency Maybe thatrsquos why

Tapco has the two most popular designs in the world Tapco hasa bucket that will interchange and replace existing buckets to giveyou the durability you need to handle abrasive loadshellipwith equal or greater capacity

When you call Tapco yoursquoll be pleased to learnthat your representative literally DOES speakyour language ndash and knows which bucketsare the best choice for your application Visitwwwtapcoinccom to find out moreabout Tapcorsquos world-class features

Thorsten Rogner Europe amp Asia(Speaks German)ldquoCC-XD ndash Toughest on the marketrdquo

Bruce Fagala USA (Speaks English)

ldquoNon-sparking and saferdquo

Jimi Davidson USA (Speaks Spanish)ldquoAvailable in polyethylenepolyurethane and nylonrdquo

FANGED HEADElevator Bolt

FLAT COUNTERSUNKHEAD (NO 1 NORWAY)

Elevator Bolt

Carl Swisher USA amp Canada

(Speaks Spanish)

ldquoXtremely efficientrdquo

Yassine Abbad Middle East amp Africa(Speaks French amp Arabic)ldquoBuckets for every applicationrdquo

John Paul Santangelo Latin America (Speaks Spanish)

ldquoStocking distributors worldwiderdquo

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ldquoSame day shipping availablerdquo

When it Comes to Elevator Bucket PerformanceTapco Speaks Your Language

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The color blue when used in connection with elevator buckets is a US registered trademark owned by Tapco Inc Super EuroBucket trade is a registered trademark of Tapco Inc copy 2010 Tapco Increg All rights reserved

SpeakAd_FINALA4 22310 102 AM Page 1

Strategic Cooperation Agreement between CPF and AKVA

Charoen Phokphand Foods Ltd the worlds leading producer and

suppl ier of farmed White Shrimp and AKVA group ASA the worlds leading supplier of technology for the aquacul-ture industry have signed a stra-tegic cooperation agreement for developing a complete new production system for the pro-duction of white shrimp utilising AKVAs recirculation technology and CPFs broad knowledge in shrimp farming

CPF is working actively to improve the i r product ion methods and technology

A natural next step for the shrimp farming industry is to utilise new technological oppor-tunities to address many of the challenges of the industry and society today

ldquoThis strategic cooperation agreement with AKVA is an important step for us in our strive

towards a transformation of the shrimp farming industry

ldquoBy th i s pro ject we are addressing impor tant issues for the industr y such as the level of which marine resource and land areas are utilised the escaping of shrimp from farms to interfere with wild stocks the control of diseases and efficient battling of parasites the elimi-nation of and risks of pollution from shrimp farms and other possible effects on the habitats where the farming is done Further to this we believe that the technology that this stra-tegic cooperation agreement will produce has the potential also to limit the carbon foot-print of the produced shrimp significantlyrdquo says Dr Chingchai Lohanawatanakul Chairman of the executive Board of CPF

The first step of the strategic cooperation includes developing a full scale prototype of a 200

tonnesyear intensive facility for white shrimp (Litopenaeus Vannamei) as an integrated solution

ldquoFor AKVA group this is an important step forward in our development in the Asian market and the furthering of our recir-culation technology in the global market

ldquoWe appreciate the initia-tives and eagerness that CPF is showing in this project and are looking forward to the results from this cooperation for both par tiesrdquo says Mr Knut Molaug CEO of AKVA group

Mo re in fo rMatio n

Knut MolaugCEOAKVA group ASA Nordlysveien 4PO Box 271 N-4349 BryneNorwayTel +47 901 39522Fax +47 51 778501Email kmolaugakvagroupcom

Back from left Mr Robins P McIntosh (Senior VP CPF) Mr Jan Erik Svensson (Regional Director AKVA group S E Asia) Dr Chingchai Lohawatanakul (Chairman of the Executive Board CPF)Front from left Mr Knut Molaug (CEO AKVA group) Dr Sujint Thammasart (Executive VP CPF)

Je f f R o g e r s h a s b e e n appointed managing director to lead Per ten Instruments

of Australia Pty Ltd He brings over 25 years expe-

rience in the scientific instrument equipment marketplace and is well qualified to lead the company in delivering our world class tech-nology and support

ldquoThis is a very exciting time for

our company We are all looking forward to a great 2010 with our customers and suppliersrdquo says Mr Rogers

He takes over from Rodney B o o t h w h o h a s r e t i r e d a s manag ing d i r ec to r and w i l l now t a ke a Bu s i ne s s Development role within the l a r ge r Per ten In s t r ument s G r o u p R o d n e y f o u n d e d N e w p o r t S c i e n t i f i c a n d l aunched the Rap id V i s co Analyser (RVA) in 1985

ldquoI am looking forward to my new focus on strategic projects a n d r e s e a r c h a n d d e ve l o p -ment of new and applied technol-ogies rdquo says Mr

Booth Mr Booth also serves on the

board of AACC International since September 2009

As par t of the in tegr at ion of Newport Scientific i n t o Pe r t e n I n s t r u m e n t s the legal entity Newport Scientific P t y L t d h a s changed name and is now Perten Instruments of Australia Pty Ltd

The company will continue to provide sales applications and service suppor t for the full range of Swedish and Australian made Per ten products Their focus on local manufactur ing and development of analyt-ical equipment such as the RVA and dough LAB remains unchanged

Mo re in fo rMatio n

Jeff RogersManaging Director Perten Instruments of Australia Pty LtdUnit 1 2 Apollo Street Warriewood NSW 2102 AustraliaTel +61 2 99796992Fax +61 2 99796993Email jrogerspertencomWebsite wwwpertencom

Perten Instruments appoints managing director in Australia

Jeff Rogers Rodney Booth

4 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 5

Aqua News

ILDEX Vietnam 2010 is ready to offer technology and business solutions for Vietnamrsquos live-

stock dairy meat-processing and aquaculture industries according to NEO - the organizer of ILDEX Vietnam 2010 which will take place on March 25-27 2010 at the New Saigon Exhibition and Convention Center Ho Chi Minh City Vietnam

NCC Exhibition Organizer Co Ltd (NEO) announced the read-iness of ILDEX Vietnam 2010 which will take place on March 25-27 2010 at the New Saigon Exhibit ion and Convention Center in Ho Chi Minh City Vietnam

At the Kick-off Launching of ILDEX Vietnam 2010 Ms Ladda Mongkolchaivivat general manager NCC Exh ib i t ion Organizer (NEO) told industry representative that ldquoILDEX Vietnam 2010 will be the largest livestock exhibition in Vietnam It will be a technology showcase of 125 companies from 22 coun-tries including country pavilion from France Singapore Korea China Shanghai City and the Netherlands

ldquoA number of international governmental and industr ial bodies such as Korean Animal Health Products Association Canadian Swine Association

British Pig Association Vietnam Depar tment of Animal Health V i e t n am Depa r tmen t o f Livestock Production and CCPIT Shanghai have confirmed their par ticipation at this industrial eventrdquo

Wi t h heav y p romot i on campaign and full government suppor t especially from the Ministry of Agricultural and Rural Development of Vietnam NEO believes that ILDEX Vietnam 2010 will attract a lot of traders and buyers from over 46 cities and provinces surrounding Ho Chi Minh City Free shuttle service will be provided for trade visitors from those areas

ILDEX Vietnam 2010 comes in perfect timing as Vietnam live-stock dair y meat-processing and aquaculture industries are searching for new technology and business solutions to move the industry to the next level

Aiming to provide the industry representative with clearer perspective of Vietnam live-stock and aquaculture indus-tries ILDEX invited Professor Hoang Kim Giao director of depar tment of livestock pro-duction Ministry of Agriculture and Rural Development and Mr Nguyen Van Trong vice director-general Aquaculture Research Institute II of Vietnam to give

keynote speeches on ldquoHusbandry in Vietnam ndash Current Status and Development Goalsrdquo and ldquoOver view Aquaculture in Vietnamrdquo respectively

Professor Hoang Kim Giao under lined the impor tant of Vietnam livestock industry

However he said ldquoIt is time for Vietnam to change the struc-ture of its agriculture and rural development Priority should be given to development of live-stock industry including industri-alization of livestock farming and food production management to produce high-quality livestock products that meet international food safety standard and enough to serve rising domestic and export demands Equally impor-tant is to protect the natural envi-ronment for sustainable develop-mentrdquo

Mr Nguyen Van Trong echoed the needs for technology and modern business solutions for Vietnam aquaculture industry Although fish and shrimp have been growing rapidly both have been facing continuous challenges especially rising production costs lowering prices and disease man-agement

ldquoOur aquaculture industr y might be growing a little too fast especially for catfish farming

ldquoThe industr y must ser i -

ously look into sustainable fa rming management Th i s t ime around the emphasis should be on quality rather than quantity to ensure that growth will continue at sus-tainable pace Pangasius for example we are having market problems so while expanding into new markets we need to be cer tain that Vietnamrsquos products meet safety standards and food hygiene required by impor tersrdquo

Apar t from technology and business solutions on the exhi-bition floors ILDEX Vietnam Aquaculture Conference 2010 under the theme lsquothe new dimen-sions for Vietnam Aquaculture Industryrsquo will be a good educa-tion forum for stakeholders along the production chain from pondhatchery operators to technical level personnel to managers feed manufacturers government officers to grasp the issues facing by the industry and develop suitable policies and aquaculture suppliers supporting the industry

Mo re in fo rMatio n

ILDEX Vietnam 2010 and ILDEX Vietnam Aquaculture Conference 2010Mr Lertwat Chanthatarath Senior Sales Manager Tel +66 2 20342614Email lertwatQsncccomWebsite wwwildexcom

ILDEX Vietnam 2010 the solutions for Vietnam sustainable growth

4 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 5

Aqua News

AE2010 is organised by the European Aquaculture Society and hosted by

the Portuguese Marine Sciences Associate Laboratory CIMAR (CIIMAR Por to and CCMAR Faro)

Portugal and PortoAquaculture production in

Por tugal is modest with some 10000 tonnes of production of which more than half is shell-fish

Marine aquaculture in Portugal is mainly centred on the Algarve coast but more recently land-based production of turbot and other marine species has devel-oped on the Atlantic coast

Currently there is renewed interest in developing aqua-culture in Por tugalrsquos outer-most regions notably in the Azores The Portuguese remain Europersquos highest consumers of fish and shellfish with annual per capita consumption of close to 60kg

Por to is Por tugalrsquos second biggest city and the lsquocapital of the nor thrsquo Its historic centre was declared a World Heritage Site by UNESCO in 1996 and it shared the designation European Culture Capital in 2001The city has excellent flight connections with flag and low cost carriers and road connections with Galicia

and especially with Vigo just 90 minutes away

AE2010 will take place at the Centro de Congressos da Alfacircndega ndash the old customs house on the quay of the Douro River in the heart of Porto and just opposite the famous por t wine cellars that are synonymous with this lively city These will form part of the AE2010 social pro-gramme

An extensive conference programme

The thematic sessions take place each morning of AE2010 and include plenary presentations by invited speakers

They pave the way for the parallel technical sessions of con-tributed presentations in both oral and poster format

A vision for the future status of marine aquaculture in Europe effects of a changing environment on biodiversity and physiology of farmed species Changing envi-ronment is at the heart of the European concerns what will be the impacts at short and medium terms For aquaculture pro-ducing specific species not able to maintain a constant body tem-perature (ectotherms) various influences are expected both for species geographical distribu-tion and so adequacy between species and present rearing sites

and impacts on species physi-ology The European aquaculture in mainly producing molluscs and fishes rapidly developed in several countries in taking into account the interdependences between species physiological needs and local conditions in terms of both more or less sheltered areas and environmental factors changes In a situation of rapid environmental changes what are the measures we have to take to follow them maybe to forecast them and to better prepare the aquacul-ture of tomorrow This review proposes to analyse this new sit-uation Presented by Gilles Boeuf President of the French National Museum of Natural History

A license to produce In the light of the new Commission strategy for the development of European aquaculture what type of gover-nance and regulatory framework is needed What should be the roles of public authorities and NGOrsquos in the development of the sector Presented by Javier Ojeda Apromar Spain

Competing claims ndash joint solu-tions How to integrate aquacul-ture with other coastal activities how can aquaculture contribute to the maritime strategy to coastal zone management and to Natura 2000 objectives Presented by Franccedilois Simard IUCN Global Marine Programme

Consumer demands and con-sumption trends What are the demand and consumption trends and what do European con-sumers prefer How important to consumers is the origin and certification of aquatic products produced lsquolocallyrsquo in Europe or impor ted from third coun-tr ies Presented by Philippe Paquotte DG Mare European Commission

Sustainable sea farming How do we develop sustainable sea farming using the ecosystem approach How is aquaculture linked with natural ecosystem processes and with patrimonial cultivation Presented by Joatildeo Gomes Ferreira New University of Lisbon Portugal

AE2010 is now calling for abstracts for presentation in parallel and poster sessions

These sessions are organised in several groups as follows

bull Hatchery production and spec ies d iver s i f i ca t ion including sessions on

bull Finfish hatcher y produc-tion covering all aspects of juvenile production proc-esses and management

bull Shellfish (juvenile) produc-tion including new develop-ments in breeding hatchery production natural spat col-lection

bull Management of aquaculture

The future of marine aquaculture

Aquaculture Europe 2010 to be held in Porto Portugal from October 5-8 2010 puts the focus on the future of marine aquaculture in Europe

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

stocks including reproduc-tion breeding and genetic improvements

bull Species diversification - finfish including the culture of flatfish tuna and other lsquonewrsquo finfish species for future commercial production

bull Species diversification ndash other species including the culture of octopus cuttlefish abalone crabs seaweeds and other lsquonewrsquo species for future commercial production

bull Production in different envi-ronments and produc-tion technologies including sessions on

bull Land-based production - new production technol-ogies including large-scale RAS production in ar id regions and other land based activities

bull Offshore aquaculture of fish and shellfish

bull Aquaculture on Islands ndash specificities of production in outlying areas in tropical and temperate regions including European outer-most regions

Tools for the development of the sector including sessions on

bull The eco-system approach to aquaculture produc-tion including semi inten-siveextensive coastallagoon aquaculture nutrient man-agement and IMTA invasive species predator control nature management and pat-rimonial conservation

bull Space for aquaculture Carrying capacity and Spatial Planning

bull Aquaculture governance and interactions with fish-eries including policy soci-oeconomic and economic research and interactions commonalities and interde-pendence with fisheries

bull Health quality and well-being including sessions on

bull Human health consumer science and quality including

aquaculture percept ion studies biotoxins quality assessment and detection certification

bull Health management of finfish and shellfish Sponsored by Inter vet Schering-Plough Animal Health

bull Novel feeds ingredients and additives Sponsored by Biomin

bull Aquaculture welfare and ethics

The conference will also include a special Student Workshop and naturally a student reception

Science meets industryAquaculture Europe 2010 will

also feature a special interna-tional trade exhibition organised by the European Aquaculture Society Por tuguese Spanish and International companies will present the latest products and ser vices for aquacul-ture A special farmersrsquo day programme will have an emphasis on aquaculture in Por tugal and in Spain and will focus on topics where the latest research findings can have an impact on the sector

It is hoped that the main Por tuguese and Spanish industr y associations for finfish and shel l fish wil l take advantage of the farmersrsquo day to bring their members to Por to and to use the event for their own meetings

Good local and industry support

AE2010 i s f inanc ia l ly suppor ted by the Area Metropolitana do Porto

The gold sponsor of AE2010 is Sorgal SA

E A S i s p r o u d t o announce the AE2010 Gold Sponsorship of Portuguese a q u a f e e d c o m p a n y Aquasoja Aquasoja is the fish feed brand of Sorgal SA Sorgal SA is par t of a

large Por tuguese agro-indus-trial group Sorgal has produced quality feeds for animal farming for more than 50 years and has always conducted its activities focussing on cost-effectiveness animal welfare and feed safety as top priorities

As par t o f i t s Premier Sponsorship of the European Aquaculture Society Inter vet Schering Plough Animal Health will have a strong presence and will sponsor the health manage-ment session

Feed ingredients and addi-t ives specia l i s t B iomin wi l l sponsor the session on Novel feeds ingredients and addi-tives

Those interested in booking exhibition space andor sponsor-ship opportunities should contact Mario Stael at mariostaelscarletbe

EU to showcase FP6 research

The EU Forum ndash a regular feature of Aquaculture Europe events ndash is currently being planned and will look to showcase the results of two of the biggest integrated research projects carried out by the Commission in aquaculture as well as presenting the view of national research funders The EU Forum will also look forward to the final stages of FP7

Just pr ior to AE2010 the Larvanet network will meet in Porto The latest findings of their research will be presented in a special session within the confer-ence A series of short workshops organised by other EU-funded projects and other relevant ini-tiatives will also take place in the exhibition area and specifically targeted towards exhibitors and trade show visitors

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

Kiotechagil the interna-tional supplier of natural high performance feed

additives to enhance growth health and sustainability in agri-culture and aquaculture will have a strong presence at VIV in The Netherlands in April 2010

The company will be high-lighting their new acidifier pHorce which compliments their range of acid products pHorce will provide a concen-trated acid on a micropearl silica carrier and can be used for decontamination of raw materials as an alternative to

c o r r o s i v e liquids and in finished feed at one kgtonne

In addition Kiotechagil will be showcasing a new broad spectrum anti-mycotoxin product which will combine the benefits of their highly effective alumino-silicate Sorbatox with a primary growth yeast cell wall compo-nent high in both beta glucans and mannan oligosaccharides

Mo re in fo rMatio n

Kiotechagil VIV Stand No C066 in Hall 9Tel +44 1189 813333Email infokiotechagilcom

Kiotechagil to show pHorceful presence at VIV

Animal nutrition company Norel launched a new website wwwnorel

es that will provide up-to-date information about its products and the company activities

Founded in 1980 by Mr Enrique Pablos Perez Norel SA has been steadily growing and developing into a well known brand The original meaning of Norel was the Spanish phrase lsquoNO REconocemos Limitesrsquo l i teral ly meaning lsquoWe donrsquot acknowledge limitsrsquo thus dem-onstrating Norelrsquos philosophy The Skyrsquos The Limit or in other words the possibi l i t ies are endless

Norel has become associated with dynamism and innovation (more than five percent of its turnover is invested in Research and Development) and also with quality and performance thanks to our ISO GMP and FAMI-QS certifications

An important feature of Norel is its focus since the beginning on animal nutrition

Originally Norel was a supplier of raw mater ials for animal nutrition milk replacers whey products and protein extracts In 1982 it star ted the production

of spray fat concentrates as well as organic trace minerals (methionates)

Expansion and develop-ment followed and in 1988 Norel opened i ts b io-technology plant in Leon (Spain)

ldquo We t h e n b e c a m e the fi r st b iotechnology company in Spain spe-cialised in animal nutrition In 1990 we star ted the production of by-pass fats for ruminants Today with our flagship Magnapac we are one of the major pro-ducers worldwide Our devel-opment continued with new factories in Egypt and Mexico and specialized units in Spainrdquo says the company in an inter-view with the press

ldquoAn impor tant date in our history is certainly 2001 when Norel SA merged its activities with Nature SA a company spe-cialized in the production of flavours and sweeteners acidi-fiers antioxidants and mould inhibitors

ldquoWe then became known in the market as lsquoNorel amp Naturersquo at that time it was impor tant for us to keep the name Nature

because our act iv it ies and products were different but at the same time very complemen-tary Today we are pleased to introduce our new logo together with the launch of our brand new website wwwnoreles

The new logo of Norel takes into account the histor y by combin ing the two Nrsquos of Norel and Nature Together they resemble the famous Yin Yang symbol and remind us that the two entities now make one The new logo also represents the infinity symbol a reference to the or ig inal meaning of Norel continue to

grow and the possibilities are endless

I t s new webs i t e comes w i th an improved cor po-rate identity and many new features and updates Most impor tant ly i t has added a news section that wil l keep reader s updated on la test developments and activ it ies of Norel worldwide

Mo re in fo rMatio n

Norel SAJesus Aprendiz 19 1 A y BMadrid NC 28007 SpainTel +34 6698 09659Website wwwnorelnaturecom

NOREL announces new web site and new corporate image

8 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 9

Dishman Group o f Ahmedabad I nd i a a lead ing products

and ser vices provider to the pharmaceutical industr y has announced the appointment of Chris Oates to the position of chief operating officer

Mr Oates will repor t to Nick Green president of the Dishman Globa l Contr act Research and Manufactur ing Ser vices (CRAMS) business

M r O a t e s w i l l a s s u m e responsibil ity for Indian and Chinese operations within the company

He wi l l be managing the development operation and improvement of the systems in the two manufacturing sites in India (Bavla and Naroda) and

the new Shanghai facility which is due for completion mid-2010

In addition to the units in Naroda and China Mr Oates wil l be responsible for the nine operating units at Bavla including a large-scale high potency fac i l i ty which wi l l be inaugurated at the end of January The new state-of-the-ar t high potency facil ity will extend Carbogen Amcisrsquo man-ufactur ing capacity to multi-tonne scale and will be the first of its kind to be located in Asia

M r O a t e s rsquo e x p e r i e n c e combines both product ion and operational roles in the contract manufacturing arena He will play a pivotal role in

expanding the companyrsquos core CRAMS business

Mr Oates holds a bach-elorrsquos degree in applied chem-istr y from the University of Huddersfield in the UK His exper t ise is bui lt on more than 20 years working within the pharmaceutical industr y He most recently ser ved as managing director of Uquifa Mexico His previous expe-r ience includes six year s at Avecia Pharmaceuticals in the UK where he held the position of operations manager and as the position of senior pro-duction manager for Syngenta (Agrochemicals)

ldquo I am looking forward to taking up this new challenge and am very excited by what I

believe will be a very successful future for the companyrdquo said Mr Oates

ldquoDishman can provide the full continuum of ser vices from process RampD to large-scale commercial manufacturing in multiple locations around the globe The company is ideally p l aced to bu i ld upon i t s success to date and become the industr y leading CRAMS par tnerrdquo

Mo re in fo rMatio n

Dishman Europe LimitedSuite 4 De Walden Court 85 New Cavendish Street London W1W 6XDUnited KingdomTel +44 2073 230608 Fax +44 2073 230609Website wwwdishmangroupcom

Dishman appoints Chris Oates as chief operating officerNew role will oversee Dishman Indian and Chinese Facilities

Sponsorship of a keynote session at Aquaculture 2010 confirms Biominrsquos

firm commitment and keen focus on this dynamic growth sector

Biomin embraces the new year with a bold plunge in aqua-culture

Fo r the f i r s t t ime the company wil l be par tner ing the World Aquaculture Society (WAS) with the sponsorship of the Aquafeeds Session at Aquaculture 2010

To be held in San Diego from March 1-5 2010 Aquaculture 2010 is the largest event for the global aquaculture industry Hosted every year by the WAS the global aquaculture event is expected to draw some 4000 participants from over 80 coun-tr ies including high profi le

attendees who form the core of decision-makers and innova-tors in the aquatic business

Biomin is proud to be a session sponsor at the worldrsquos largest aquaculture event Held on March 4 2010 the session on lsquoAquafeedsrsquo will see exper t speakers from com-mercial practice and academia share their i n s i gh t s on ma jor issues in aquafeeds formulation and alter-nat ives to fishmeal and protein replace-ment strategies for a variety of aquatic species

Years of dedicated applied research and investments have strengthened Biominrsquos drive and reputation in providing effective natural-based feed solutions for the aquaculture and aquafeed

business throughout the world A core focus on fundamental applied research forms the basis of Biominrsquos portfolio of science-based aquaculture product solu-tions Biominreg AquaSpecials which includes nutraceuticals probiotics natural growth pro-

moters mycotoxin deactiva-tors and functional feed addi-tives

Dr Pedro Encarnaccedilatildeo Biominrsquos aquacul ture spec ia l i s t says ldquoBiomin is a research-focused and -driven company

ldquoWe have translated our long-

standing competence in animal nutrition for terrestrial species into aquaculture and aquafeed research Our Aquatic Centre for Applied Animals (AQUACAN) in Thailand actively conducts feeding trials for many aquatic species from which new findings

have been translated into innovative and practical solutions in aquafeedsrdquo

Biomin will also par-ticipate as a sponsor at the Australas ian Aquacu l ture 2010

another WAS event to be held in Hobar t Australia in May 2010 and at the Aquaculture Europe that will take place in Por to Por tugal in October 2010 both of which are key events for aquaculture in the coming year

A bold step for BIOMIN aquacultureBiomin proud to be session sponsor at the worldrsquos largest aquaculture event

8 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 9

Aqua News

Tecnoloacutegica de Alimentos SA (TASA) of Peru is the first fishmeal and fish

oil producer to achieve cer ti-fication under the new IFFO Global Standard for Responsible Supply

The cer tification has been awarded to the Callao Nor te plant of TASA the worldrsquos largest fishmeal producer Earlier this week Jorge Mora Operations Director of IFFO presented the

IFFO-Assured certificate to Mario Brescia CEO of TASA at its head-quarters in Lima Peru

IFFO has been developing its RS Standard since early 2008 with the help of a Technical Advisory Committee comprising repre-sentatives from the whole aqua-culture value chain including fish farmers feed producers and retailers and also NGOs

This business-to-business inde-pendently-audited programme will enable IFFO members to demonstrate to their customers that they are offer ing pure marine ingredients manufactured from responsibly sourced and traceable raw materials Whole fish used must come from fish-eries managed under the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

ldquoSince October 2009 when we launched the programme we have had applications from 15 manufacturing companies in four major producing countries sourcing from 10 fisheriesrdquo says IFFO director general Jonathan Shepherd

ldquoWe anticipate that there will be substantial supplies of IFFO-Assured fishmeal and fish oil on the market within months and that up to one third of global

p r o d u c t i o n could be certi-fied by the end of 2010rdquo

ldquoWith th i s p r o g r a m m e IFFO has put in place the first link in a fully certified supply chain for aqua-culture and for land animals such as pigs and poul tr y

It will help the industry demon-strate its commitment to respon-sible manufacture of fishmeal and fish oil as well as to the FAO Code of Conductrdquo said Dr Shepherd

Applicant factories which can demonstrate that they are ldquooper-ating a well managed system that ensures their marine raw mate-rials are responsibly sourced and processed to the IFFO Standardrdquo will be awarded certification

They will be able to offer IFFO-Assured fishmeal and fish oil and have the right to display the RS logo An up-to-date list of cur-rently certified fishmeal plants will be posted on the IFFO web site alongside the full Standard

Characteristics of the IFFO Global Standard for Responsible Supply (RS)

bull Standards set by a multi-stake-holder Technical Advisory

Committee including pro-ducers traders fish farmers feed manufacturers proc-essors retailers standard setting organisations and environmental NGOs The Assessment Management and Certification Process are contracted to Global Trust

bull A programme independ-ently administered by an ISO 651996 accredited certifica-tion body which is a member of the International Accreditation Forum (IFA)

bull I t d e m o n s t r a t e s responsible sourcing o f t r aceable r aw material and respon-sible production to ensure feed safety

bull A business-to-busi-ness tool designed to enable fishmeal and fish oil producers to demonstrate the integr i ty o f the i r products and their processes to their cus-tomers in the value-chain rather than a consumer eco-label

bull Whole fish used must come from fisheries that have been independently assessed and meet the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

bull Exclusion of illegal unre-por ted and unregulated (IUU) fish

bull The factor y must have attained Cer tification to the Internat ional Feed Ingredients Standard (IFIS) of the International Feed Safety Alliance (IFSA) or equiva-lent as proof of responsible manufacturing and the appli-cant must be in possession

of all relevant permits and licenses for the production and sale of fishmeal and fish oil products

bull This programme is com-patible with ISO 651996 (General requirements for bodies operating product cer tification systems) and with EN45011

bull Recogn i ses other cer-t i f i c a t ion p rog r ammes w h i c h h av e d e m o n -

strated equivalency and which are accepted within the industry - eg Marine Stewardship Council (MSC) for the fishery certification element only

The IFFO programme will continue to be developed in 2010 addressing further aspects of responsible supply

Mo re in fo rMatio n

Andrew JacksonTechnical Director IFFO 2 College YardLower Dagnall Street St Albans Hertfordshire AL3 4PAUnited KingdomTel +44 7808 783488 Email ajacksoniffonetWebiste wwwiffonet

First certification awarded under the IFFO Global Standard for Responsible Supply

ldquoThe first link in a fully certified aquaculture supply chain is in placerdquo

ldquoSupplies of ldquoIFFO-Assuredrdquo fishmeal and fish oil will shortly be on the marketrdquo

Jorge Mora (right) Operations Director of IFFO presenting the IFFO-Assured certificate to Mario Brescia (left) CEO of TASA at its headquarters in Lima Peru

10 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 11

Me r c a t o r M e d i a o r g a n i s e r o f t h e Offshore Mariculture

Conference 2010 is pleased to announce that the leading i ndus t r y a s soc i a t ions w i l l be suppor t i n g t he 2010 Conference

The European Aquaculture Techno logy amp I nnova t ion Platform (EATiP) the Federation o f Eu ropean Aquacu l tu re Producers (FEAP) the Global

Aquaculture All iance (GAA) and HGK Grupacija akvakul-ture the aquaculture affi l ia-tion of the Croatian Chamber of Economy (and member of FEAP) have all pledged their suppor t to the Conference that wi l l be held from the

June 16-18 2010 at the Hilton Imper ial Hotel Dubrovnik Croatia

Both EATiP (European Aquaculture Technology amp Innovation Platform) and FEAP (Federation of European Aquaculture Producers) are pleased to suppor t the Offshore Mariculture Conference s ince events such as these give fish farmers t h e o p p o r t u -n i t y t o l e a r n from both indus-t r i a l and RTD

exper ts about relevant technological advances to rece ive in formed updates on key research and policy issues and

to be able to network with col leagues from across the sector says Cour tney Hough general secretary of FEAP and EATiP

As wel l as promoting the event to their members and therefore rais ing awareness of the event and its presti-gious speakers it also means that members of any of the above assoc ia t ions wi l l be en t i t l ed to a 10 pe rcen t d i s count o f f the de lega te rate European Aquaculture Society members will also be ent i t led to the discount as the EAS have continued their suppor t f rom the prev ious even t he ld i n A l i c an te i n 2008

The technical conference will be held on the June 16 and 17 June with a visit to a fish farm on the June 18 2010 The Conference Networking Dinner will take place on the evening of Wednesday June 16 2010 at the fabulous Revelin For tress which was bui lt in 1462 to provide protection to the Eastern City Gate Place Over look ing the Old Ci ty Harbour the Revelins terrace offers what are probably the best views of the Old City and its famous Harbour

Mo re in fo rMatio n

Offshore MaricultureTel +44 1622 820622Website wwwoffshoremariculturecom

Industry support strengthens for 2010 OSM Conference

The full programme will be released shortly sessions will include

bull Policy legal framework and key research updates

bull Practical offshore farming operation

bull Spatial planning and moni-toring

bull Spotlight on Croatiabull Ecological monitoring and

future technologiesbull European and International

case studies

iN THE NEWSYour news could feature in our pages

Do you have an news item that you would like to see in our pages Send your press releases to the Production Manager - Nicky BarnesEmail nickybaquafeedcouk

10 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 11

Aqua News

The aquaculture industry today faces a number of challenges that threaten to restrict its development

and profitability

To live in harmony with consumer expec-tations of the highest possible standards of product quality and to do so in a sustainable and environmentally responsible manner necessitates an innovative coordinated and holistic approach within the aquaculture industry

The requirement for sustainability has focused on the composition of the feed being used as one of the key parameters determining the sustainability of production of a particular species particularly the fish-meal and fish oil content and how it relates to overall feed conversion ratios

Further formulation trends are moving towards low fishmeal and oil content in the grower feeds and finishing feeds designed to restore flesh quality parameters such as omega 3 fats and fillet quality that are con-sidered essential for the promotion of the product and its benefits for human health

This strategy addresses to some extent the limited resources of fishmeal and fish oil

Expectations of sustainabilityThe only way to achieve the expecta-

tions of sustainability and replace or supple-ment fishmeal and fish oil in aquafeeds is to exploit alternative protein and lipid sources

The obvious and traditionally used alter-natives are animal and plant protein sources

However in Europe and now increas-ingly in North America consumers pre-fer aquaculture products to have been

fed without the use of animal by-products due in part to recent health scares in the terrestrial animal sec-tor such as bovine spongiform encepha-lopathy (BSE)

The search is on therefore for not only the simple replacement of protein but the provi-sion of functional nutri-tion addressing both the sustainability and flesh quality requirements of the industry while

meeting the nutritional requirement of the animal being cultured

Alternative vegetable protein sources

Over the last two decades aquaculture research has focused on plant protein sources such as grains pulses and oilseeds as they are generally widely available

However the use of soybean meal and other plant based protein sources such as legume seeds oilseed cakes leaf meals leaf protein concentrates and root tuber meals have been limited due to the presence of a wide variety of anti-nutritional substances in these products and their interaction with the physiology of the animal resulting in depressed growth and feed efficiency mortality and abnormal intestinal morphol-ogy and damage

These substances act in different ways by affecting protein utilisation and digestion (eg protease inhibitors tannins lectins) by affecting mineral utilisation (eg phytate gossypol pigments glucosinolates) antivita-mins and other miscellaneous substances (eg phytoestrogens)

The increasing use of plant protein sources is also raising new concerns about possible contamination by mould derived mycotoxins

This risk is enhanced by the global nature and trading of plant protein sourc-es and the as yet little investigated impact of different mycotoxins and their interac-tions in aquaculture species In 2008 the Rapid Alert System for Food and Feed in

Protein sources Alternative protein sources should also show functionality if they are to play a role in modern aquafeedsby Keith Filer PhD research Manager Asia-Pacific biosciences Centre Alltech inc

Figure 1 A comparison of typical essential amino acid (EAA) requirements for fish and the EAA profile of AquateTM

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

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the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

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12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

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have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

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retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

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conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

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Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

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16 | InternatIonal AquAFeed | March-april 2010

Alltechreg has developed a range of products to address dietary requirements at different

stages of life in aquaculture species These sustainable product solutions are proven to

support profitability in aquaculture operations as well as performance in fish and shrimp

in the Family

Give your fish and shrimp the Alltech Aqua Advantage Contact your Alltech representative today for more information

trade

aquasolutionsalltechcomwwwalltechcom

Ideally suited for fish and shrimp in any stage of life to maintain performance and to support independence from traditional aquaculture diet ingredients

Specifically designed for the juvenile stages of life in fish and shrimp to support good health uniform growth and survival

A natural affordable product created for salmon in all life stages to maintain good health and defense mechanisms against adverse aquatic elements present in the surrounding environment

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Aquate Ad for IAF due Feb 19 2010V2indd 1 2192010 115722 AM

Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

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Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

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of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

8 page full colour document - 100 copies = UKpound489 (please add UKpound15 for every additional 100 copies)

Delivery charged on a case by case basis

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1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

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(UKpound15 per copy)

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Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

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Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

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Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

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Media Partners

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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Services (publications)

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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If you have a product or service that you want to advertise but we do not have a heading let us know

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 5: March | April 10 - International Aquafeed

2 | InternatIonal AquAFeed | March-april 2010

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(Speaks English)

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gentle handlingrdquo

Wherever your product is processed storedor shipped one thing is constantthe

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Since 1974 Tapco buckets havebeen outperforming all othersin tests and actual usage ndashhandling everything from

feed to fertilizer with care andefficiency Maybe thatrsquos why

Tapco has the two most popular designs in the world Tapco hasa bucket that will interchange and replace existing buckets to giveyou the durability you need to handle abrasive loadshellipwith equal or greater capacity

When you call Tapco yoursquoll be pleased to learnthat your representative literally DOES speakyour language ndash and knows which bucketsare the best choice for your application Visitwwwtapcoinccom to find out moreabout Tapcorsquos world-class features

Thorsten Rogner Europe amp Asia(Speaks German)ldquoCC-XD ndash Toughest on the marketrdquo

Bruce Fagala USA (Speaks English)

ldquoNon-sparking and saferdquo

Jimi Davidson USA (Speaks Spanish)ldquoAvailable in polyethylenepolyurethane and nylonrdquo

FANGED HEADElevator Bolt

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Elevator Bolt

Carl Swisher USA amp Canada

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ldquoXtremely efficientrdquo

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When it Comes to Elevator Bucket PerformanceTapco Speaks Your Language

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Strategic Cooperation Agreement between CPF and AKVA

Charoen Phokphand Foods Ltd the worlds leading producer and

suppl ier of farmed White Shrimp and AKVA group ASA the worlds leading supplier of technology for the aquacul-ture industry have signed a stra-tegic cooperation agreement for developing a complete new production system for the pro-duction of white shrimp utilising AKVAs recirculation technology and CPFs broad knowledge in shrimp farming

CPF is working actively to improve the i r product ion methods and technology

A natural next step for the shrimp farming industry is to utilise new technological oppor-tunities to address many of the challenges of the industry and society today

ldquoThis strategic cooperation agreement with AKVA is an important step for us in our strive

towards a transformation of the shrimp farming industry

ldquoBy th i s pro ject we are addressing impor tant issues for the industr y such as the level of which marine resource and land areas are utilised the escaping of shrimp from farms to interfere with wild stocks the control of diseases and efficient battling of parasites the elimi-nation of and risks of pollution from shrimp farms and other possible effects on the habitats where the farming is done Further to this we believe that the technology that this stra-tegic cooperation agreement will produce has the potential also to limit the carbon foot-print of the produced shrimp significantlyrdquo says Dr Chingchai Lohanawatanakul Chairman of the executive Board of CPF

The first step of the strategic cooperation includes developing a full scale prototype of a 200

tonnesyear intensive facility for white shrimp (Litopenaeus Vannamei) as an integrated solution

ldquoFor AKVA group this is an important step forward in our development in the Asian market and the furthering of our recir-culation technology in the global market

ldquoWe appreciate the initia-tives and eagerness that CPF is showing in this project and are looking forward to the results from this cooperation for both par tiesrdquo says Mr Knut Molaug CEO of AKVA group

Mo re in fo rMatio n

Knut MolaugCEOAKVA group ASA Nordlysveien 4PO Box 271 N-4349 BryneNorwayTel +47 901 39522Fax +47 51 778501Email kmolaugakvagroupcom

Back from left Mr Robins P McIntosh (Senior VP CPF) Mr Jan Erik Svensson (Regional Director AKVA group S E Asia) Dr Chingchai Lohawatanakul (Chairman of the Executive Board CPF)Front from left Mr Knut Molaug (CEO AKVA group) Dr Sujint Thammasart (Executive VP CPF)

Je f f R o g e r s h a s b e e n appointed managing director to lead Per ten Instruments

of Australia Pty Ltd He brings over 25 years expe-

rience in the scientific instrument equipment marketplace and is well qualified to lead the company in delivering our world class tech-nology and support

ldquoThis is a very exciting time for

our company We are all looking forward to a great 2010 with our customers and suppliersrdquo says Mr Rogers

He takes over from Rodney B o o t h w h o h a s r e t i r e d a s manag ing d i r ec to r and w i l l now t a ke a Bu s i ne s s Development role within the l a r ge r Per ten In s t r ument s G r o u p R o d n e y f o u n d e d N e w p o r t S c i e n t i f i c a n d l aunched the Rap id V i s co Analyser (RVA) in 1985

ldquoI am looking forward to my new focus on strategic projects a n d r e s e a r c h a n d d e ve l o p -ment of new and applied technol-ogies rdquo says Mr

Booth Mr Booth also serves on the

board of AACC International since September 2009

As par t of the in tegr at ion of Newport Scientific i n t o Pe r t e n I n s t r u m e n t s the legal entity Newport Scientific P t y L t d h a s changed name and is now Perten Instruments of Australia Pty Ltd

The company will continue to provide sales applications and service suppor t for the full range of Swedish and Australian made Per ten products Their focus on local manufactur ing and development of analyt-ical equipment such as the RVA and dough LAB remains unchanged

Mo re in fo rMatio n

Jeff RogersManaging Director Perten Instruments of Australia Pty LtdUnit 1 2 Apollo Street Warriewood NSW 2102 AustraliaTel +61 2 99796992Fax +61 2 99796993Email jrogerspertencomWebsite wwwpertencom

Perten Instruments appoints managing director in Australia

Jeff Rogers Rodney Booth

4 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 5

Aqua News

ILDEX Vietnam 2010 is ready to offer technology and business solutions for Vietnamrsquos live-

stock dairy meat-processing and aquaculture industries according to NEO - the organizer of ILDEX Vietnam 2010 which will take place on March 25-27 2010 at the New Saigon Exhibition and Convention Center Ho Chi Minh City Vietnam

NCC Exhibition Organizer Co Ltd (NEO) announced the read-iness of ILDEX Vietnam 2010 which will take place on March 25-27 2010 at the New Saigon Exhibit ion and Convention Center in Ho Chi Minh City Vietnam

At the Kick-off Launching of ILDEX Vietnam 2010 Ms Ladda Mongkolchaivivat general manager NCC Exh ib i t ion Organizer (NEO) told industry representative that ldquoILDEX Vietnam 2010 will be the largest livestock exhibition in Vietnam It will be a technology showcase of 125 companies from 22 coun-tries including country pavilion from France Singapore Korea China Shanghai City and the Netherlands

ldquoA number of international governmental and industr ial bodies such as Korean Animal Health Products Association Canadian Swine Association

British Pig Association Vietnam Depar tment of Animal Health V i e t n am Depa r tmen t o f Livestock Production and CCPIT Shanghai have confirmed their par ticipation at this industrial eventrdquo

Wi t h heav y p romot i on campaign and full government suppor t especially from the Ministry of Agricultural and Rural Development of Vietnam NEO believes that ILDEX Vietnam 2010 will attract a lot of traders and buyers from over 46 cities and provinces surrounding Ho Chi Minh City Free shuttle service will be provided for trade visitors from those areas

ILDEX Vietnam 2010 comes in perfect timing as Vietnam live-stock dair y meat-processing and aquaculture industries are searching for new technology and business solutions to move the industry to the next level

Aiming to provide the industry representative with clearer perspective of Vietnam live-stock and aquaculture indus-tries ILDEX invited Professor Hoang Kim Giao director of depar tment of livestock pro-duction Ministry of Agriculture and Rural Development and Mr Nguyen Van Trong vice director-general Aquaculture Research Institute II of Vietnam to give

keynote speeches on ldquoHusbandry in Vietnam ndash Current Status and Development Goalsrdquo and ldquoOver view Aquaculture in Vietnamrdquo respectively

Professor Hoang Kim Giao under lined the impor tant of Vietnam livestock industry

However he said ldquoIt is time for Vietnam to change the struc-ture of its agriculture and rural development Priority should be given to development of live-stock industry including industri-alization of livestock farming and food production management to produce high-quality livestock products that meet international food safety standard and enough to serve rising domestic and export demands Equally impor-tant is to protect the natural envi-ronment for sustainable develop-mentrdquo

Mr Nguyen Van Trong echoed the needs for technology and modern business solutions for Vietnam aquaculture industry Although fish and shrimp have been growing rapidly both have been facing continuous challenges especially rising production costs lowering prices and disease man-agement

ldquoOur aquaculture industr y might be growing a little too fast especially for catfish farming

ldquoThe industr y must ser i -

ously look into sustainable fa rming management Th i s t ime around the emphasis should be on quality rather than quantity to ensure that growth will continue at sus-tainable pace Pangasius for example we are having market problems so while expanding into new markets we need to be cer tain that Vietnamrsquos products meet safety standards and food hygiene required by impor tersrdquo

Apar t from technology and business solutions on the exhi-bition floors ILDEX Vietnam Aquaculture Conference 2010 under the theme lsquothe new dimen-sions for Vietnam Aquaculture Industryrsquo will be a good educa-tion forum for stakeholders along the production chain from pondhatchery operators to technical level personnel to managers feed manufacturers government officers to grasp the issues facing by the industry and develop suitable policies and aquaculture suppliers supporting the industry

Mo re in fo rMatio n

ILDEX Vietnam 2010 and ILDEX Vietnam Aquaculture Conference 2010Mr Lertwat Chanthatarath Senior Sales Manager Tel +66 2 20342614Email lertwatQsncccomWebsite wwwildexcom

ILDEX Vietnam 2010 the solutions for Vietnam sustainable growth

4 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 5

Aqua News

AE2010 is organised by the European Aquaculture Society and hosted by

the Portuguese Marine Sciences Associate Laboratory CIMAR (CIIMAR Por to and CCMAR Faro)

Portugal and PortoAquaculture production in

Por tugal is modest with some 10000 tonnes of production of which more than half is shell-fish

Marine aquaculture in Portugal is mainly centred on the Algarve coast but more recently land-based production of turbot and other marine species has devel-oped on the Atlantic coast

Currently there is renewed interest in developing aqua-culture in Por tugalrsquos outer-most regions notably in the Azores The Portuguese remain Europersquos highest consumers of fish and shellfish with annual per capita consumption of close to 60kg

Por to is Por tugalrsquos second biggest city and the lsquocapital of the nor thrsquo Its historic centre was declared a World Heritage Site by UNESCO in 1996 and it shared the designation European Culture Capital in 2001The city has excellent flight connections with flag and low cost carriers and road connections with Galicia

and especially with Vigo just 90 minutes away

AE2010 will take place at the Centro de Congressos da Alfacircndega ndash the old customs house on the quay of the Douro River in the heart of Porto and just opposite the famous por t wine cellars that are synonymous with this lively city These will form part of the AE2010 social pro-gramme

An extensive conference programme

The thematic sessions take place each morning of AE2010 and include plenary presentations by invited speakers

They pave the way for the parallel technical sessions of con-tributed presentations in both oral and poster format

A vision for the future status of marine aquaculture in Europe effects of a changing environment on biodiversity and physiology of farmed species Changing envi-ronment is at the heart of the European concerns what will be the impacts at short and medium terms For aquaculture pro-ducing specific species not able to maintain a constant body tem-perature (ectotherms) various influences are expected both for species geographical distribu-tion and so adequacy between species and present rearing sites

and impacts on species physi-ology The European aquaculture in mainly producing molluscs and fishes rapidly developed in several countries in taking into account the interdependences between species physiological needs and local conditions in terms of both more or less sheltered areas and environmental factors changes In a situation of rapid environmental changes what are the measures we have to take to follow them maybe to forecast them and to better prepare the aquacul-ture of tomorrow This review proposes to analyse this new sit-uation Presented by Gilles Boeuf President of the French National Museum of Natural History

A license to produce In the light of the new Commission strategy for the development of European aquaculture what type of gover-nance and regulatory framework is needed What should be the roles of public authorities and NGOrsquos in the development of the sector Presented by Javier Ojeda Apromar Spain

Competing claims ndash joint solu-tions How to integrate aquacul-ture with other coastal activities how can aquaculture contribute to the maritime strategy to coastal zone management and to Natura 2000 objectives Presented by Franccedilois Simard IUCN Global Marine Programme

Consumer demands and con-sumption trends What are the demand and consumption trends and what do European con-sumers prefer How important to consumers is the origin and certification of aquatic products produced lsquolocallyrsquo in Europe or impor ted from third coun-tr ies Presented by Philippe Paquotte DG Mare European Commission

Sustainable sea farming How do we develop sustainable sea farming using the ecosystem approach How is aquaculture linked with natural ecosystem processes and with patrimonial cultivation Presented by Joatildeo Gomes Ferreira New University of Lisbon Portugal

AE2010 is now calling for abstracts for presentation in parallel and poster sessions

These sessions are organised in several groups as follows

bull Hatchery production and spec ies d iver s i f i ca t ion including sessions on

bull Finfish hatcher y produc-tion covering all aspects of juvenile production proc-esses and management

bull Shellfish (juvenile) produc-tion including new develop-ments in breeding hatchery production natural spat col-lection

bull Management of aquaculture

The future of marine aquaculture

Aquaculture Europe 2010 to be held in Porto Portugal from October 5-8 2010 puts the focus on the future of marine aquaculture in Europe

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

stocks including reproduc-tion breeding and genetic improvements

bull Species diversification - finfish including the culture of flatfish tuna and other lsquonewrsquo finfish species for future commercial production

bull Species diversification ndash other species including the culture of octopus cuttlefish abalone crabs seaweeds and other lsquonewrsquo species for future commercial production

bull Production in different envi-ronments and produc-tion technologies including sessions on

bull Land-based production - new production technol-ogies including large-scale RAS production in ar id regions and other land based activities

bull Offshore aquaculture of fish and shellfish

bull Aquaculture on Islands ndash specificities of production in outlying areas in tropical and temperate regions including European outer-most regions

Tools for the development of the sector including sessions on

bull The eco-system approach to aquaculture produc-tion including semi inten-siveextensive coastallagoon aquaculture nutrient man-agement and IMTA invasive species predator control nature management and pat-rimonial conservation

bull Space for aquaculture Carrying capacity and Spatial Planning

bull Aquaculture governance and interactions with fish-eries including policy soci-oeconomic and economic research and interactions commonalities and interde-pendence with fisheries

bull Health quality and well-being including sessions on

bull Human health consumer science and quality including

aquaculture percept ion studies biotoxins quality assessment and detection certification

bull Health management of finfish and shellfish Sponsored by Inter vet Schering-Plough Animal Health

bull Novel feeds ingredients and additives Sponsored by Biomin

bull Aquaculture welfare and ethics

The conference will also include a special Student Workshop and naturally a student reception

Science meets industryAquaculture Europe 2010 will

also feature a special interna-tional trade exhibition organised by the European Aquaculture Society Por tuguese Spanish and International companies will present the latest products and ser vices for aquacul-ture A special farmersrsquo day programme will have an emphasis on aquaculture in Por tugal and in Spain and will focus on topics where the latest research findings can have an impact on the sector

It is hoped that the main Por tuguese and Spanish industr y associations for finfish and shel l fish wil l take advantage of the farmersrsquo day to bring their members to Por to and to use the event for their own meetings

Good local and industry support

AE2010 i s f inanc ia l ly suppor ted by the Area Metropolitana do Porto

The gold sponsor of AE2010 is Sorgal SA

E A S i s p r o u d t o announce the AE2010 Gold Sponsorship of Portuguese a q u a f e e d c o m p a n y Aquasoja Aquasoja is the fish feed brand of Sorgal SA Sorgal SA is par t of a

large Por tuguese agro-indus-trial group Sorgal has produced quality feeds for animal farming for more than 50 years and has always conducted its activities focussing on cost-effectiveness animal welfare and feed safety as top priorities

As par t o f i t s Premier Sponsorship of the European Aquaculture Society Inter vet Schering Plough Animal Health will have a strong presence and will sponsor the health manage-ment session

Feed ingredients and addi-t ives specia l i s t B iomin wi l l sponsor the session on Novel feeds ingredients and addi-tives

Those interested in booking exhibition space andor sponsor-ship opportunities should contact Mario Stael at mariostaelscarletbe

EU to showcase FP6 research

The EU Forum ndash a regular feature of Aquaculture Europe events ndash is currently being planned and will look to showcase the results of two of the biggest integrated research projects carried out by the Commission in aquaculture as well as presenting the view of national research funders The EU Forum will also look forward to the final stages of FP7

Just pr ior to AE2010 the Larvanet network will meet in Porto The latest findings of their research will be presented in a special session within the confer-ence A series of short workshops organised by other EU-funded projects and other relevant ini-tiatives will also take place in the exhibition area and specifically targeted towards exhibitors and trade show visitors

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

Kiotechagil the interna-tional supplier of natural high performance feed

additives to enhance growth health and sustainability in agri-culture and aquaculture will have a strong presence at VIV in The Netherlands in April 2010

The company will be high-lighting their new acidifier pHorce which compliments their range of acid products pHorce will provide a concen-trated acid on a micropearl silica carrier and can be used for decontamination of raw materials as an alternative to

c o r r o s i v e liquids and in finished feed at one kgtonne

In addition Kiotechagil will be showcasing a new broad spectrum anti-mycotoxin product which will combine the benefits of their highly effective alumino-silicate Sorbatox with a primary growth yeast cell wall compo-nent high in both beta glucans and mannan oligosaccharides

Mo re in fo rMatio n

Kiotechagil VIV Stand No C066 in Hall 9Tel +44 1189 813333Email infokiotechagilcom

Kiotechagil to show pHorceful presence at VIV

Animal nutrition company Norel launched a new website wwwnorel

es that will provide up-to-date information about its products and the company activities

Founded in 1980 by Mr Enrique Pablos Perez Norel SA has been steadily growing and developing into a well known brand The original meaning of Norel was the Spanish phrase lsquoNO REconocemos Limitesrsquo l i teral ly meaning lsquoWe donrsquot acknowledge limitsrsquo thus dem-onstrating Norelrsquos philosophy The Skyrsquos The Limit or in other words the possibi l i t ies are endless

Norel has become associated with dynamism and innovation (more than five percent of its turnover is invested in Research and Development) and also with quality and performance thanks to our ISO GMP and FAMI-QS certifications

An important feature of Norel is its focus since the beginning on animal nutrition

Originally Norel was a supplier of raw mater ials for animal nutrition milk replacers whey products and protein extracts In 1982 it star ted the production

of spray fat concentrates as well as organic trace minerals (methionates)

Expansion and develop-ment followed and in 1988 Norel opened i ts b io-technology plant in Leon (Spain)

ldquo We t h e n b e c a m e the fi r st b iotechnology company in Spain spe-cialised in animal nutrition In 1990 we star ted the production of by-pass fats for ruminants Today with our flagship Magnapac we are one of the major pro-ducers worldwide Our devel-opment continued with new factories in Egypt and Mexico and specialized units in Spainrdquo says the company in an inter-view with the press

ldquoAn impor tant date in our history is certainly 2001 when Norel SA merged its activities with Nature SA a company spe-cialized in the production of flavours and sweeteners acidi-fiers antioxidants and mould inhibitors

ldquoWe then became known in the market as lsquoNorel amp Naturersquo at that time it was impor tant for us to keep the name Nature

because our act iv it ies and products were different but at the same time very complemen-tary Today we are pleased to introduce our new logo together with the launch of our brand new website wwwnoreles

The new logo of Norel takes into account the histor y by combin ing the two Nrsquos of Norel and Nature Together they resemble the famous Yin Yang symbol and remind us that the two entities now make one The new logo also represents the infinity symbol a reference to the or ig inal meaning of Norel continue to

grow and the possibilities are endless

I t s new webs i t e comes w i th an improved cor po-rate identity and many new features and updates Most impor tant ly i t has added a news section that wil l keep reader s updated on la test developments and activ it ies of Norel worldwide

Mo re in fo rMatio n

Norel SAJesus Aprendiz 19 1 A y BMadrid NC 28007 SpainTel +34 6698 09659Website wwwnorelnaturecom

NOREL announces new web site and new corporate image

8 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 9

Dishman Group o f Ahmedabad I nd i a a lead ing products

and ser vices provider to the pharmaceutical industr y has announced the appointment of Chris Oates to the position of chief operating officer

Mr Oates will repor t to Nick Green president of the Dishman Globa l Contr act Research and Manufactur ing Ser vices (CRAMS) business

M r O a t e s w i l l a s s u m e responsibil ity for Indian and Chinese operations within the company

He wi l l be managing the development operation and improvement of the systems in the two manufacturing sites in India (Bavla and Naroda) and

the new Shanghai facility which is due for completion mid-2010

In addition to the units in Naroda and China Mr Oates wil l be responsible for the nine operating units at Bavla including a large-scale high potency fac i l i ty which wi l l be inaugurated at the end of January The new state-of-the-ar t high potency facil ity will extend Carbogen Amcisrsquo man-ufactur ing capacity to multi-tonne scale and will be the first of its kind to be located in Asia

M r O a t e s rsquo e x p e r i e n c e combines both product ion and operational roles in the contract manufacturing arena He will play a pivotal role in

expanding the companyrsquos core CRAMS business

Mr Oates holds a bach-elorrsquos degree in applied chem-istr y from the University of Huddersfield in the UK His exper t ise is bui lt on more than 20 years working within the pharmaceutical industr y He most recently ser ved as managing director of Uquifa Mexico His previous expe-r ience includes six year s at Avecia Pharmaceuticals in the UK where he held the position of operations manager and as the position of senior pro-duction manager for Syngenta (Agrochemicals)

ldquo I am looking forward to taking up this new challenge and am very excited by what I

believe will be a very successful future for the companyrdquo said Mr Oates

ldquoDishman can provide the full continuum of ser vices from process RampD to large-scale commercial manufacturing in multiple locations around the globe The company is ideally p l aced to bu i ld upon i t s success to date and become the industr y leading CRAMS par tnerrdquo

Mo re in fo rMatio n

Dishman Europe LimitedSuite 4 De Walden Court 85 New Cavendish Street London W1W 6XDUnited KingdomTel +44 2073 230608 Fax +44 2073 230609Website wwwdishmangroupcom

Dishman appoints Chris Oates as chief operating officerNew role will oversee Dishman Indian and Chinese Facilities

Sponsorship of a keynote session at Aquaculture 2010 confirms Biominrsquos

firm commitment and keen focus on this dynamic growth sector

Biomin embraces the new year with a bold plunge in aqua-culture

Fo r the f i r s t t ime the company wil l be par tner ing the World Aquaculture Society (WAS) with the sponsorship of the Aquafeeds Session at Aquaculture 2010

To be held in San Diego from March 1-5 2010 Aquaculture 2010 is the largest event for the global aquaculture industry Hosted every year by the WAS the global aquaculture event is expected to draw some 4000 participants from over 80 coun-tr ies including high profi le

attendees who form the core of decision-makers and innova-tors in the aquatic business

Biomin is proud to be a session sponsor at the worldrsquos largest aquaculture event Held on March 4 2010 the session on lsquoAquafeedsrsquo will see exper t speakers from com-mercial practice and academia share their i n s i gh t s on ma jor issues in aquafeeds formulation and alter-nat ives to fishmeal and protein replace-ment strategies for a variety of aquatic species

Years of dedicated applied research and investments have strengthened Biominrsquos drive and reputation in providing effective natural-based feed solutions for the aquaculture and aquafeed

business throughout the world A core focus on fundamental applied research forms the basis of Biominrsquos portfolio of science-based aquaculture product solu-tions Biominreg AquaSpecials which includes nutraceuticals probiotics natural growth pro-

moters mycotoxin deactiva-tors and functional feed addi-tives

Dr Pedro Encarnaccedilatildeo Biominrsquos aquacul ture spec ia l i s t says ldquoBiomin is a research-focused and -driven company

ldquoWe have translated our long-

standing competence in animal nutrition for terrestrial species into aquaculture and aquafeed research Our Aquatic Centre for Applied Animals (AQUACAN) in Thailand actively conducts feeding trials for many aquatic species from which new findings

have been translated into innovative and practical solutions in aquafeedsrdquo

Biomin will also par-ticipate as a sponsor at the Australas ian Aquacu l ture 2010

another WAS event to be held in Hobar t Australia in May 2010 and at the Aquaculture Europe that will take place in Por to Por tugal in October 2010 both of which are key events for aquaculture in the coming year

A bold step for BIOMIN aquacultureBiomin proud to be session sponsor at the worldrsquos largest aquaculture event

8 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 9

Aqua News

Tecnoloacutegica de Alimentos SA (TASA) of Peru is the first fishmeal and fish

oil producer to achieve cer ti-fication under the new IFFO Global Standard for Responsible Supply

The cer tification has been awarded to the Callao Nor te plant of TASA the worldrsquos largest fishmeal producer Earlier this week Jorge Mora Operations Director of IFFO presented the

IFFO-Assured certificate to Mario Brescia CEO of TASA at its head-quarters in Lima Peru

IFFO has been developing its RS Standard since early 2008 with the help of a Technical Advisory Committee comprising repre-sentatives from the whole aqua-culture value chain including fish farmers feed producers and retailers and also NGOs

This business-to-business inde-pendently-audited programme will enable IFFO members to demonstrate to their customers that they are offer ing pure marine ingredients manufactured from responsibly sourced and traceable raw materials Whole fish used must come from fish-eries managed under the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

ldquoSince October 2009 when we launched the programme we have had applications from 15 manufacturing companies in four major producing countries sourcing from 10 fisheriesrdquo says IFFO director general Jonathan Shepherd

ldquoWe anticipate that there will be substantial supplies of IFFO-Assured fishmeal and fish oil on the market within months and that up to one third of global

p r o d u c t i o n could be certi-fied by the end of 2010rdquo

ldquoWith th i s p r o g r a m m e IFFO has put in place the first link in a fully certified supply chain for aqua-culture and for land animals such as pigs and poul tr y

It will help the industry demon-strate its commitment to respon-sible manufacture of fishmeal and fish oil as well as to the FAO Code of Conductrdquo said Dr Shepherd

Applicant factories which can demonstrate that they are ldquooper-ating a well managed system that ensures their marine raw mate-rials are responsibly sourced and processed to the IFFO Standardrdquo will be awarded certification

They will be able to offer IFFO-Assured fishmeal and fish oil and have the right to display the RS logo An up-to-date list of cur-rently certified fishmeal plants will be posted on the IFFO web site alongside the full Standard

Characteristics of the IFFO Global Standard for Responsible Supply (RS)

bull Standards set by a multi-stake-holder Technical Advisory

Committee including pro-ducers traders fish farmers feed manufacturers proc-essors retailers standard setting organisations and environmental NGOs The Assessment Management and Certification Process are contracted to Global Trust

bull A programme independ-ently administered by an ISO 651996 accredited certifica-tion body which is a member of the International Accreditation Forum (IFA)

bull I t d e m o n s t r a t e s responsible sourcing o f t r aceable r aw material and respon-sible production to ensure feed safety

bull A business-to-busi-ness tool designed to enable fishmeal and fish oil producers to demonstrate the integr i ty o f the i r products and their processes to their cus-tomers in the value-chain rather than a consumer eco-label

bull Whole fish used must come from fisheries that have been independently assessed and meet the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

bull Exclusion of illegal unre-por ted and unregulated (IUU) fish

bull The factor y must have attained Cer tification to the Internat ional Feed Ingredients Standard (IFIS) of the International Feed Safety Alliance (IFSA) or equiva-lent as proof of responsible manufacturing and the appli-cant must be in possession

of all relevant permits and licenses for the production and sale of fishmeal and fish oil products

bull This programme is com-patible with ISO 651996 (General requirements for bodies operating product cer tification systems) and with EN45011

bull Recogn i ses other cer-t i f i c a t ion p rog r ammes w h i c h h av e d e m o n -

strated equivalency and which are accepted within the industry - eg Marine Stewardship Council (MSC) for the fishery certification element only

The IFFO programme will continue to be developed in 2010 addressing further aspects of responsible supply

Mo re in fo rMatio n

Andrew JacksonTechnical Director IFFO 2 College YardLower Dagnall Street St Albans Hertfordshire AL3 4PAUnited KingdomTel +44 7808 783488 Email ajacksoniffonetWebiste wwwiffonet

First certification awarded under the IFFO Global Standard for Responsible Supply

ldquoThe first link in a fully certified aquaculture supply chain is in placerdquo

ldquoSupplies of ldquoIFFO-Assuredrdquo fishmeal and fish oil will shortly be on the marketrdquo

Jorge Mora (right) Operations Director of IFFO presenting the IFFO-Assured certificate to Mario Brescia (left) CEO of TASA at its headquarters in Lima Peru

10 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 11

Me r c a t o r M e d i a o r g a n i s e r o f t h e Offshore Mariculture

Conference 2010 is pleased to announce that the leading i ndus t r y a s soc i a t ions w i l l be suppor t i n g t he 2010 Conference

The European Aquaculture Techno logy amp I nnova t ion Platform (EATiP) the Federation o f Eu ropean Aquacu l tu re Producers (FEAP) the Global

Aquaculture All iance (GAA) and HGK Grupacija akvakul-ture the aquaculture affi l ia-tion of the Croatian Chamber of Economy (and member of FEAP) have all pledged their suppor t to the Conference that wi l l be held from the

June 16-18 2010 at the Hilton Imper ial Hotel Dubrovnik Croatia

Both EATiP (European Aquaculture Technology amp Innovation Platform) and FEAP (Federation of European Aquaculture Producers) are pleased to suppor t the Offshore Mariculture Conference s ince events such as these give fish farmers t h e o p p o r t u -n i t y t o l e a r n from both indus-t r i a l and RTD

exper ts about relevant technological advances to rece ive in formed updates on key research and policy issues and

to be able to network with col leagues from across the sector says Cour tney Hough general secretary of FEAP and EATiP

As wel l as promoting the event to their members and therefore rais ing awareness of the event and its presti-gious speakers it also means that members of any of the above assoc ia t ions wi l l be en t i t l ed to a 10 pe rcen t d i s count o f f the de lega te rate European Aquaculture Society members will also be ent i t led to the discount as the EAS have continued their suppor t f rom the prev ious even t he ld i n A l i c an te i n 2008

The technical conference will be held on the June 16 and 17 June with a visit to a fish farm on the June 18 2010 The Conference Networking Dinner will take place on the evening of Wednesday June 16 2010 at the fabulous Revelin For tress which was bui lt in 1462 to provide protection to the Eastern City Gate Place Over look ing the Old Ci ty Harbour the Revelins terrace offers what are probably the best views of the Old City and its famous Harbour

Mo re in fo rMatio n

Offshore MaricultureTel +44 1622 820622Website wwwoffshoremariculturecom

Industry support strengthens for 2010 OSM Conference

The full programme will be released shortly sessions will include

bull Policy legal framework and key research updates

bull Practical offshore farming operation

bull Spatial planning and moni-toring

bull Spotlight on Croatiabull Ecological monitoring and

future technologiesbull European and International

case studies

iN THE NEWSYour news could feature in our pages

Do you have an news item that you would like to see in our pages Send your press releases to the Production Manager - Nicky BarnesEmail nickybaquafeedcouk

10 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 11

Aqua News

The aquaculture industry today faces a number of challenges that threaten to restrict its development

and profitability

To live in harmony with consumer expec-tations of the highest possible standards of product quality and to do so in a sustainable and environmentally responsible manner necessitates an innovative coordinated and holistic approach within the aquaculture industry

The requirement for sustainability has focused on the composition of the feed being used as one of the key parameters determining the sustainability of production of a particular species particularly the fish-meal and fish oil content and how it relates to overall feed conversion ratios

Further formulation trends are moving towards low fishmeal and oil content in the grower feeds and finishing feeds designed to restore flesh quality parameters such as omega 3 fats and fillet quality that are con-sidered essential for the promotion of the product and its benefits for human health

This strategy addresses to some extent the limited resources of fishmeal and fish oil

Expectations of sustainabilityThe only way to achieve the expecta-

tions of sustainability and replace or supple-ment fishmeal and fish oil in aquafeeds is to exploit alternative protein and lipid sources

The obvious and traditionally used alter-natives are animal and plant protein sources

However in Europe and now increas-ingly in North America consumers pre-fer aquaculture products to have been

fed without the use of animal by-products due in part to recent health scares in the terrestrial animal sec-tor such as bovine spongiform encepha-lopathy (BSE)

The search is on therefore for not only the simple replacement of protein but the provi-sion of functional nutri-tion addressing both the sustainability and flesh quality requirements of the industry while

meeting the nutritional requirement of the animal being cultured

Alternative vegetable protein sources

Over the last two decades aquaculture research has focused on plant protein sources such as grains pulses and oilseeds as they are generally widely available

However the use of soybean meal and other plant based protein sources such as legume seeds oilseed cakes leaf meals leaf protein concentrates and root tuber meals have been limited due to the presence of a wide variety of anti-nutritional substances in these products and their interaction with the physiology of the animal resulting in depressed growth and feed efficiency mortality and abnormal intestinal morphol-ogy and damage

These substances act in different ways by affecting protein utilisation and digestion (eg protease inhibitors tannins lectins) by affecting mineral utilisation (eg phytate gossypol pigments glucosinolates) antivita-mins and other miscellaneous substances (eg phytoestrogens)

The increasing use of plant protein sources is also raising new concerns about possible contamination by mould derived mycotoxins

This risk is enhanced by the global nature and trading of plant protein sourc-es and the as yet little investigated impact of different mycotoxins and their interac-tions in aquaculture species In 2008 the Rapid Alert System for Food and Feed in

Protein sources Alternative protein sources should also show functionality if they are to play a role in modern aquafeedsby Keith Filer PhD research Manager Asia-Pacific biosciences Centre Alltech inc

Figure 1 A comparison of typical essential amino acid (EAA) requirements for fish and the EAA profile of AquateTM

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

juns

wor

th |

Dre

amst

ime

com

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

040

120

08

180

120

08

010

220

08

150

220

08

290

220

08

140

320

08

280

320

08

110

420

08

250

420

08

090

520

08

230

520

08

16 | InternatIonal AquAFeed | March-april 2010

Alltechreg has developed a range of products to address dietary requirements at different

stages of life in aquaculture species These sustainable product solutions are proven to

support profitability in aquaculture operations as well as performance in fish and shrimp

in the Family

Give your fish and shrimp the Alltech Aqua Advantage Contact your Alltech representative today for more information

trade

aquasolutionsalltechcomwwwalltechcom

Ideally suited for fish and shrimp in any stage of life to maintain performance and to support independence from traditional aquaculture diet ingredients

Specifically designed for the juvenile stages of life in fish and shrimp to support good health uniform growth and survival

A natural affordable product created for salmon in all life stages to maintain good health and defense mechanisms against adverse aquatic elements present in the surrounding environment

trade

trade

Aquate Ad for IAF due Feb 19 2010V2indd 1 2192010 115722 AM

Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

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20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

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1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

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(UKpound15 per copy)

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Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

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Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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If you have a product or service that you want to advertise but we do not have a heading let us know

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 6: March | April 10 - International Aquafeed

Strategic Cooperation Agreement between CPF and AKVA

Charoen Phokphand Foods Ltd the worlds leading producer and

suppl ier of farmed White Shrimp and AKVA group ASA the worlds leading supplier of technology for the aquacul-ture industry have signed a stra-tegic cooperation agreement for developing a complete new production system for the pro-duction of white shrimp utilising AKVAs recirculation technology and CPFs broad knowledge in shrimp farming

CPF is working actively to improve the i r product ion methods and technology

A natural next step for the shrimp farming industry is to utilise new technological oppor-tunities to address many of the challenges of the industry and society today

ldquoThis strategic cooperation agreement with AKVA is an important step for us in our strive

towards a transformation of the shrimp farming industry

ldquoBy th i s pro ject we are addressing impor tant issues for the industr y such as the level of which marine resource and land areas are utilised the escaping of shrimp from farms to interfere with wild stocks the control of diseases and efficient battling of parasites the elimi-nation of and risks of pollution from shrimp farms and other possible effects on the habitats where the farming is done Further to this we believe that the technology that this stra-tegic cooperation agreement will produce has the potential also to limit the carbon foot-print of the produced shrimp significantlyrdquo says Dr Chingchai Lohanawatanakul Chairman of the executive Board of CPF

The first step of the strategic cooperation includes developing a full scale prototype of a 200

tonnesyear intensive facility for white shrimp (Litopenaeus Vannamei) as an integrated solution

ldquoFor AKVA group this is an important step forward in our development in the Asian market and the furthering of our recir-culation technology in the global market

ldquoWe appreciate the initia-tives and eagerness that CPF is showing in this project and are looking forward to the results from this cooperation for both par tiesrdquo says Mr Knut Molaug CEO of AKVA group

Mo re in fo rMatio n

Knut MolaugCEOAKVA group ASA Nordlysveien 4PO Box 271 N-4349 BryneNorwayTel +47 901 39522Fax +47 51 778501Email kmolaugakvagroupcom

Back from left Mr Robins P McIntosh (Senior VP CPF) Mr Jan Erik Svensson (Regional Director AKVA group S E Asia) Dr Chingchai Lohawatanakul (Chairman of the Executive Board CPF)Front from left Mr Knut Molaug (CEO AKVA group) Dr Sujint Thammasart (Executive VP CPF)

Je f f R o g e r s h a s b e e n appointed managing director to lead Per ten Instruments

of Australia Pty Ltd He brings over 25 years expe-

rience in the scientific instrument equipment marketplace and is well qualified to lead the company in delivering our world class tech-nology and support

ldquoThis is a very exciting time for

our company We are all looking forward to a great 2010 with our customers and suppliersrdquo says Mr Rogers

He takes over from Rodney B o o t h w h o h a s r e t i r e d a s manag ing d i r ec to r and w i l l now t a ke a Bu s i ne s s Development role within the l a r ge r Per ten In s t r ument s G r o u p R o d n e y f o u n d e d N e w p o r t S c i e n t i f i c a n d l aunched the Rap id V i s co Analyser (RVA) in 1985

ldquoI am looking forward to my new focus on strategic projects a n d r e s e a r c h a n d d e ve l o p -ment of new and applied technol-ogies rdquo says Mr

Booth Mr Booth also serves on the

board of AACC International since September 2009

As par t of the in tegr at ion of Newport Scientific i n t o Pe r t e n I n s t r u m e n t s the legal entity Newport Scientific P t y L t d h a s changed name and is now Perten Instruments of Australia Pty Ltd

The company will continue to provide sales applications and service suppor t for the full range of Swedish and Australian made Per ten products Their focus on local manufactur ing and development of analyt-ical equipment such as the RVA and dough LAB remains unchanged

Mo re in fo rMatio n

Jeff RogersManaging Director Perten Instruments of Australia Pty LtdUnit 1 2 Apollo Street Warriewood NSW 2102 AustraliaTel +61 2 99796992Fax +61 2 99796993Email jrogerspertencomWebsite wwwpertencom

Perten Instruments appoints managing director in Australia

Jeff Rogers Rodney Booth

4 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 5

Aqua News

ILDEX Vietnam 2010 is ready to offer technology and business solutions for Vietnamrsquos live-

stock dairy meat-processing and aquaculture industries according to NEO - the organizer of ILDEX Vietnam 2010 which will take place on March 25-27 2010 at the New Saigon Exhibition and Convention Center Ho Chi Minh City Vietnam

NCC Exhibition Organizer Co Ltd (NEO) announced the read-iness of ILDEX Vietnam 2010 which will take place on March 25-27 2010 at the New Saigon Exhibit ion and Convention Center in Ho Chi Minh City Vietnam

At the Kick-off Launching of ILDEX Vietnam 2010 Ms Ladda Mongkolchaivivat general manager NCC Exh ib i t ion Organizer (NEO) told industry representative that ldquoILDEX Vietnam 2010 will be the largest livestock exhibition in Vietnam It will be a technology showcase of 125 companies from 22 coun-tries including country pavilion from France Singapore Korea China Shanghai City and the Netherlands

ldquoA number of international governmental and industr ial bodies such as Korean Animal Health Products Association Canadian Swine Association

British Pig Association Vietnam Depar tment of Animal Health V i e t n am Depa r tmen t o f Livestock Production and CCPIT Shanghai have confirmed their par ticipation at this industrial eventrdquo

Wi t h heav y p romot i on campaign and full government suppor t especially from the Ministry of Agricultural and Rural Development of Vietnam NEO believes that ILDEX Vietnam 2010 will attract a lot of traders and buyers from over 46 cities and provinces surrounding Ho Chi Minh City Free shuttle service will be provided for trade visitors from those areas

ILDEX Vietnam 2010 comes in perfect timing as Vietnam live-stock dair y meat-processing and aquaculture industries are searching for new technology and business solutions to move the industry to the next level

Aiming to provide the industry representative with clearer perspective of Vietnam live-stock and aquaculture indus-tries ILDEX invited Professor Hoang Kim Giao director of depar tment of livestock pro-duction Ministry of Agriculture and Rural Development and Mr Nguyen Van Trong vice director-general Aquaculture Research Institute II of Vietnam to give

keynote speeches on ldquoHusbandry in Vietnam ndash Current Status and Development Goalsrdquo and ldquoOver view Aquaculture in Vietnamrdquo respectively

Professor Hoang Kim Giao under lined the impor tant of Vietnam livestock industry

However he said ldquoIt is time for Vietnam to change the struc-ture of its agriculture and rural development Priority should be given to development of live-stock industry including industri-alization of livestock farming and food production management to produce high-quality livestock products that meet international food safety standard and enough to serve rising domestic and export demands Equally impor-tant is to protect the natural envi-ronment for sustainable develop-mentrdquo

Mr Nguyen Van Trong echoed the needs for technology and modern business solutions for Vietnam aquaculture industry Although fish and shrimp have been growing rapidly both have been facing continuous challenges especially rising production costs lowering prices and disease man-agement

ldquoOur aquaculture industr y might be growing a little too fast especially for catfish farming

ldquoThe industr y must ser i -

ously look into sustainable fa rming management Th i s t ime around the emphasis should be on quality rather than quantity to ensure that growth will continue at sus-tainable pace Pangasius for example we are having market problems so while expanding into new markets we need to be cer tain that Vietnamrsquos products meet safety standards and food hygiene required by impor tersrdquo

Apar t from technology and business solutions on the exhi-bition floors ILDEX Vietnam Aquaculture Conference 2010 under the theme lsquothe new dimen-sions for Vietnam Aquaculture Industryrsquo will be a good educa-tion forum for stakeholders along the production chain from pondhatchery operators to technical level personnel to managers feed manufacturers government officers to grasp the issues facing by the industry and develop suitable policies and aquaculture suppliers supporting the industry

Mo re in fo rMatio n

ILDEX Vietnam 2010 and ILDEX Vietnam Aquaculture Conference 2010Mr Lertwat Chanthatarath Senior Sales Manager Tel +66 2 20342614Email lertwatQsncccomWebsite wwwildexcom

ILDEX Vietnam 2010 the solutions for Vietnam sustainable growth

4 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 5

Aqua News

AE2010 is organised by the European Aquaculture Society and hosted by

the Portuguese Marine Sciences Associate Laboratory CIMAR (CIIMAR Por to and CCMAR Faro)

Portugal and PortoAquaculture production in

Por tugal is modest with some 10000 tonnes of production of which more than half is shell-fish

Marine aquaculture in Portugal is mainly centred on the Algarve coast but more recently land-based production of turbot and other marine species has devel-oped on the Atlantic coast

Currently there is renewed interest in developing aqua-culture in Por tugalrsquos outer-most regions notably in the Azores The Portuguese remain Europersquos highest consumers of fish and shellfish with annual per capita consumption of close to 60kg

Por to is Por tugalrsquos second biggest city and the lsquocapital of the nor thrsquo Its historic centre was declared a World Heritage Site by UNESCO in 1996 and it shared the designation European Culture Capital in 2001The city has excellent flight connections with flag and low cost carriers and road connections with Galicia

and especially with Vigo just 90 minutes away

AE2010 will take place at the Centro de Congressos da Alfacircndega ndash the old customs house on the quay of the Douro River in the heart of Porto and just opposite the famous por t wine cellars that are synonymous with this lively city These will form part of the AE2010 social pro-gramme

An extensive conference programme

The thematic sessions take place each morning of AE2010 and include plenary presentations by invited speakers

They pave the way for the parallel technical sessions of con-tributed presentations in both oral and poster format

A vision for the future status of marine aquaculture in Europe effects of a changing environment on biodiversity and physiology of farmed species Changing envi-ronment is at the heart of the European concerns what will be the impacts at short and medium terms For aquaculture pro-ducing specific species not able to maintain a constant body tem-perature (ectotherms) various influences are expected both for species geographical distribu-tion and so adequacy between species and present rearing sites

and impacts on species physi-ology The European aquaculture in mainly producing molluscs and fishes rapidly developed in several countries in taking into account the interdependences between species physiological needs and local conditions in terms of both more or less sheltered areas and environmental factors changes In a situation of rapid environmental changes what are the measures we have to take to follow them maybe to forecast them and to better prepare the aquacul-ture of tomorrow This review proposes to analyse this new sit-uation Presented by Gilles Boeuf President of the French National Museum of Natural History

A license to produce In the light of the new Commission strategy for the development of European aquaculture what type of gover-nance and regulatory framework is needed What should be the roles of public authorities and NGOrsquos in the development of the sector Presented by Javier Ojeda Apromar Spain

Competing claims ndash joint solu-tions How to integrate aquacul-ture with other coastal activities how can aquaculture contribute to the maritime strategy to coastal zone management and to Natura 2000 objectives Presented by Franccedilois Simard IUCN Global Marine Programme

Consumer demands and con-sumption trends What are the demand and consumption trends and what do European con-sumers prefer How important to consumers is the origin and certification of aquatic products produced lsquolocallyrsquo in Europe or impor ted from third coun-tr ies Presented by Philippe Paquotte DG Mare European Commission

Sustainable sea farming How do we develop sustainable sea farming using the ecosystem approach How is aquaculture linked with natural ecosystem processes and with patrimonial cultivation Presented by Joatildeo Gomes Ferreira New University of Lisbon Portugal

AE2010 is now calling for abstracts for presentation in parallel and poster sessions

These sessions are organised in several groups as follows

bull Hatchery production and spec ies d iver s i f i ca t ion including sessions on

bull Finfish hatcher y produc-tion covering all aspects of juvenile production proc-esses and management

bull Shellfish (juvenile) produc-tion including new develop-ments in breeding hatchery production natural spat col-lection

bull Management of aquaculture

The future of marine aquaculture

Aquaculture Europe 2010 to be held in Porto Portugal from October 5-8 2010 puts the focus on the future of marine aquaculture in Europe

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

stocks including reproduc-tion breeding and genetic improvements

bull Species diversification - finfish including the culture of flatfish tuna and other lsquonewrsquo finfish species for future commercial production

bull Species diversification ndash other species including the culture of octopus cuttlefish abalone crabs seaweeds and other lsquonewrsquo species for future commercial production

bull Production in different envi-ronments and produc-tion technologies including sessions on

bull Land-based production - new production technol-ogies including large-scale RAS production in ar id regions and other land based activities

bull Offshore aquaculture of fish and shellfish

bull Aquaculture on Islands ndash specificities of production in outlying areas in tropical and temperate regions including European outer-most regions

Tools for the development of the sector including sessions on

bull The eco-system approach to aquaculture produc-tion including semi inten-siveextensive coastallagoon aquaculture nutrient man-agement and IMTA invasive species predator control nature management and pat-rimonial conservation

bull Space for aquaculture Carrying capacity and Spatial Planning

bull Aquaculture governance and interactions with fish-eries including policy soci-oeconomic and economic research and interactions commonalities and interde-pendence with fisheries

bull Health quality and well-being including sessions on

bull Human health consumer science and quality including

aquaculture percept ion studies biotoxins quality assessment and detection certification

bull Health management of finfish and shellfish Sponsored by Inter vet Schering-Plough Animal Health

bull Novel feeds ingredients and additives Sponsored by Biomin

bull Aquaculture welfare and ethics

The conference will also include a special Student Workshop and naturally a student reception

Science meets industryAquaculture Europe 2010 will

also feature a special interna-tional trade exhibition organised by the European Aquaculture Society Por tuguese Spanish and International companies will present the latest products and ser vices for aquacul-ture A special farmersrsquo day programme will have an emphasis on aquaculture in Por tugal and in Spain and will focus on topics where the latest research findings can have an impact on the sector

It is hoped that the main Por tuguese and Spanish industr y associations for finfish and shel l fish wil l take advantage of the farmersrsquo day to bring their members to Por to and to use the event for their own meetings

Good local and industry support

AE2010 i s f inanc ia l ly suppor ted by the Area Metropolitana do Porto

The gold sponsor of AE2010 is Sorgal SA

E A S i s p r o u d t o announce the AE2010 Gold Sponsorship of Portuguese a q u a f e e d c o m p a n y Aquasoja Aquasoja is the fish feed brand of Sorgal SA Sorgal SA is par t of a

large Por tuguese agro-indus-trial group Sorgal has produced quality feeds for animal farming for more than 50 years and has always conducted its activities focussing on cost-effectiveness animal welfare and feed safety as top priorities

As par t o f i t s Premier Sponsorship of the European Aquaculture Society Inter vet Schering Plough Animal Health will have a strong presence and will sponsor the health manage-ment session

Feed ingredients and addi-t ives specia l i s t B iomin wi l l sponsor the session on Novel feeds ingredients and addi-tives

Those interested in booking exhibition space andor sponsor-ship opportunities should contact Mario Stael at mariostaelscarletbe

EU to showcase FP6 research

The EU Forum ndash a regular feature of Aquaculture Europe events ndash is currently being planned and will look to showcase the results of two of the biggest integrated research projects carried out by the Commission in aquaculture as well as presenting the view of national research funders The EU Forum will also look forward to the final stages of FP7

Just pr ior to AE2010 the Larvanet network will meet in Porto The latest findings of their research will be presented in a special session within the confer-ence A series of short workshops organised by other EU-funded projects and other relevant ini-tiatives will also take place in the exhibition area and specifically targeted towards exhibitors and trade show visitors

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

Kiotechagil the interna-tional supplier of natural high performance feed

additives to enhance growth health and sustainability in agri-culture and aquaculture will have a strong presence at VIV in The Netherlands in April 2010

The company will be high-lighting their new acidifier pHorce which compliments their range of acid products pHorce will provide a concen-trated acid on a micropearl silica carrier and can be used for decontamination of raw materials as an alternative to

c o r r o s i v e liquids and in finished feed at one kgtonne

In addition Kiotechagil will be showcasing a new broad spectrum anti-mycotoxin product which will combine the benefits of their highly effective alumino-silicate Sorbatox with a primary growth yeast cell wall compo-nent high in both beta glucans and mannan oligosaccharides

Mo re in fo rMatio n

Kiotechagil VIV Stand No C066 in Hall 9Tel +44 1189 813333Email infokiotechagilcom

Kiotechagil to show pHorceful presence at VIV

Animal nutrition company Norel launched a new website wwwnorel

es that will provide up-to-date information about its products and the company activities

Founded in 1980 by Mr Enrique Pablos Perez Norel SA has been steadily growing and developing into a well known brand The original meaning of Norel was the Spanish phrase lsquoNO REconocemos Limitesrsquo l i teral ly meaning lsquoWe donrsquot acknowledge limitsrsquo thus dem-onstrating Norelrsquos philosophy The Skyrsquos The Limit or in other words the possibi l i t ies are endless

Norel has become associated with dynamism and innovation (more than five percent of its turnover is invested in Research and Development) and also with quality and performance thanks to our ISO GMP and FAMI-QS certifications

An important feature of Norel is its focus since the beginning on animal nutrition

Originally Norel was a supplier of raw mater ials for animal nutrition milk replacers whey products and protein extracts In 1982 it star ted the production

of spray fat concentrates as well as organic trace minerals (methionates)

Expansion and develop-ment followed and in 1988 Norel opened i ts b io-technology plant in Leon (Spain)

ldquo We t h e n b e c a m e the fi r st b iotechnology company in Spain spe-cialised in animal nutrition In 1990 we star ted the production of by-pass fats for ruminants Today with our flagship Magnapac we are one of the major pro-ducers worldwide Our devel-opment continued with new factories in Egypt and Mexico and specialized units in Spainrdquo says the company in an inter-view with the press

ldquoAn impor tant date in our history is certainly 2001 when Norel SA merged its activities with Nature SA a company spe-cialized in the production of flavours and sweeteners acidi-fiers antioxidants and mould inhibitors

ldquoWe then became known in the market as lsquoNorel amp Naturersquo at that time it was impor tant for us to keep the name Nature

because our act iv it ies and products were different but at the same time very complemen-tary Today we are pleased to introduce our new logo together with the launch of our brand new website wwwnoreles

The new logo of Norel takes into account the histor y by combin ing the two Nrsquos of Norel and Nature Together they resemble the famous Yin Yang symbol and remind us that the two entities now make one The new logo also represents the infinity symbol a reference to the or ig inal meaning of Norel continue to

grow and the possibilities are endless

I t s new webs i t e comes w i th an improved cor po-rate identity and many new features and updates Most impor tant ly i t has added a news section that wil l keep reader s updated on la test developments and activ it ies of Norel worldwide

Mo re in fo rMatio n

Norel SAJesus Aprendiz 19 1 A y BMadrid NC 28007 SpainTel +34 6698 09659Website wwwnorelnaturecom

NOREL announces new web site and new corporate image

8 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 9

Dishman Group o f Ahmedabad I nd i a a lead ing products

and ser vices provider to the pharmaceutical industr y has announced the appointment of Chris Oates to the position of chief operating officer

Mr Oates will repor t to Nick Green president of the Dishman Globa l Contr act Research and Manufactur ing Ser vices (CRAMS) business

M r O a t e s w i l l a s s u m e responsibil ity for Indian and Chinese operations within the company

He wi l l be managing the development operation and improvement of the systems in the two manufacturing sites in India (Bavla and Naroda) and

the new Shanghai facility which is due for completion mid-2010

In addition to the units in Naroda and China Mr Oates wil l be responsible for the nine operating units at Bavla including a large-scale high potency fac i l i ty which wi l l be inaugurated at the end of January The new state-of-the-ar t high potency facil ity will extend Carbogen Amcisrsquo man-ufactur ing capacity to multi-tonne scale and will be the first of its kind to be located in Asia

M r O a t e s rsquo e x p e r i e n c e combines both product ion and operational roles in the contract manufacturing arena He will play a pivotal role in

expanding the companyrsquos core CRAMS business

Mr Oates holds a bach-elorrsquos degree in applied chem-istr y from the University of Huddersfield in the UK His exper t ise is bui lt on more than 20 years working within the pharmaceutical industr y He most recently ser ved as managing director of Uquifa Mexico His previous expe-r ience includes six year s at Avecia Pharmaceuticals in the UK where he held the position of operations manager and as the position of senior pro-duction manager for Syngenta (Agrochemicals)

ldquo I am looking forward to taking up this new challenge and am very excited by what I

believe will be a very successful future for the companyrdquo said Mr Oates

ldquoDishman can provide the full continuum of ser vices from process RampD to large-scale commercial manufacturing in multiple locations around the globe The company is ideally p l aced to bu i ld upon i t s success to date and become the industr y leading CRAMS par tnerrdquo

Mo re in fo rMatio n

Dishman Europe LimitedSuite 4 De Walden Court 85 New Cavendish Street London W1W 6XDUnited KingdomTel +44 2073 230608 Fax +44 2073 230609Website wwwdishmangroupcom

Dishman appoints Chris Oates as chief operating officerNew role will oversee Dishman Indian and Chinese Facilities

Sponsorship of a keynote session at Aquaculture 2010 confirms Biominrsquos

firm commitment and keen focus on this dynamic growth sector

Biomin embraces the new year with a bold plunge in aqua-culture

Fo r the f i r s t t ime the company wil l be par tner ing the World Aquaculture Society (WAS) with the sponsorship of the Aquafeeds Session at Aquaculture 2010

To be held in San Diego from March 1-5 2010 Aquaculture 2010 is the largest event for the global aquaculture industry Hosted every year by the WAS the global aquaculture event is expected to draw some 4000 participants from over 80 coun-tr ies including high profi le

attendees who form the core of decision-makers and innova-tors in the aquatic business

Biomin is proud to be a session sponsor at the worldrsquos largest aquaculture event Held on March 4 2010 the session on lsquoAquafeedsrsquo will see exper t speakers from com-mercial practice and academia share their i n s i gh t s on ma jor issues in aquafeeds formulation and alter-nat ives to fishmeal and protein replace-ment strategies for a variety of aquatic species

Years of dedicated applied research and investments have strengthened Biominrsquos drive and reputation in providing effective natural-based feed solutions for the aquaculture and aquafeed

business throughout the world A core focus on fundamental applied research forms the basis of Biominrsquos portfolio of science-based aquaculture product solu-tions Biominreg AquaSpecials which includes nutraceuticals probiotics natural growth pro-

moters mycotoxin deactiva-tors and functional feed addi-tives

Dr Pedro Encarnaccedilatildeo Biominrsquos aquacul ture spec ia l i s t says ldquoBiomin is a research-focused and -driven company

ldquoWe have translated our long-

standing competence in animal nutrition for terrestrial species into aquaculture and aquafeed research Our Aquatic Centre for Applied Animals (AQUACAN) in Thailand actively conducts feeding trials for many aquatic species from which new findings

have been translated into innovative and practical solutions in aquafeedsrdquo

Biomin will also par-ticipate as a sponsor at the Australas ian Aquacu l ture 2010

another WAS event to be held in Hobar t Australia in May 2010 and at the Aquaculture Europe that will take place in Por to Por tugal in October 2010 both of which are key events for aquaculture in the coming year

A bold step for BIOMIN aquacultureBiomin proud to be session sponsor at the worldrsquos largest aquaculture event

8 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 9

Aqua News

Tecnoloacutegica de Alimentos SA (TASA) of Peru is the first fishmeal and fish

oil producer to achieve cer ti-fication under the new IFFO Global Standard for Responsible Supply

The cer tification has been awarded to the Callao Nor te plant of TASA the worldrsquos largest fishmeal producer Earlier this week Jorge Mora Operations Director of IFFO presented the

IFFO-Assured certificate to Mario Brescia CEO of TASA at its head-quarters in Lima Peru

IFFO has been developing its RS Standard since early 2008 with the help of a Technical Advisory Committee comprising repre-sentatives from the whole aqua-culture value chain including fish farmers feed producers and retailers and also NGOs

This business-to-business inde-pendently-audited programme will enable IFFO members to demonstrate to their customers that they are offer ing pure marine ingredients manufactured from responsibly sourced and traceable raw materials Whole fish used must come from fish-eries managed under the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

ldquoSince October 2009 when we launched the programme we have had applications from 15 manufacturing companies in four major producing countries sourcing from 10 fisheriesrdquo says IFFO director general Jonathan Shepherd

ldquoWe anticipate that there will be substantial supplies of IFFO-Assured fishmeal and fish oil on the market within months and that up to one third of global

p r o d u c t i o n could be certi-fied by the end of 2010rdquo

ldquoWith th i s p r o g r a m m e IFFO has put in place the first link in a fully certified supply chain for aqua-culture and for land animals such as pigs and poul tr y

It will help the industry demon-strate its commitment to respon-sible manufacture of fishmeal and fish oil as well as to the FAO Code of Conductrdquo said Dr Shepherd

Applicant factories which can demonstrate that they are ldquooper-ating a well managed system that ensures their marine raw mate-rials are responsibly sourced and processed to the IFFO Standardrdquo will be awarded certification

They will be able to offer IFFO-Assured fishmeal and fish oil and have the right to display the RS logo An up-to-date list of cur-rently certified fishmeal plants will be posted on the IFFO web site alongside the full Standard

Characteristics of the IFFO Global Standard for Responsible Supply (RS)

bull Standards set by a multi-stake-holder Technical Advisory

Committee including pro-ducers traders fish farmers feed manufacturers proc-essors retailers standard setting organisations and environmental NGOs The Assessment Management and Certification Process are contracted to Global Trust

bull A programme independ-ently administered by an ISO 651996 accredited certifica-tion body which is a member of the International Accreditation Forum (IFA)

bull I t d e m o n s t r a t e s responsible sourcing o f t r aceable r aw material and respon-sible production to ensure feed safety

bull A business-to-busi-ness tool designed to enable fishmeal and fish oil producers to demonstrate the integr i ty o f the i r products and their processes to their cus-tomers in the value-chain rather than a consumer eco-label

bull Whole fish used must come from fisheries that have been independently assessed and meet the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

bull Exclusion of illegal unre-por ted and unregulated (IUU) fish

bull The factor y must have attained Cer tification to the Internat ional Feed Ingredients Standard (IFIS) of the International Feed Safety Alliance (IFSA) or equiva-lent as proof of responsible manufacturing and the appli-cant must be in possession

of all relevant permits and licenses for the production and sale of fishmeal and fish oil products

bull This programme is com-patible with ISO 651996 (General requirements for bodies operating product cer tification systems) and with EN45011

bull Recogn i ses other cer-t i f i c a t ion p rog r ammes w h i c h h av e d e m o n -

strated equivalency and which are accepted within the industry - eg Marine Stewardship Council (MSC) for the fishery certification element only

The IFFO programme will continue to be developed in 2010 addressing further aspects of responsible supply

Mo re in fo rMatio n

Andrew JacksonTechnical Director IFFO 2 College YardLower Dagnall Street St Albans Hertfordshire AL3 4PAUnited KingdomTel +44 7808 783488 Email ajacksoniffonetWebiste wwwiffonet

First certification awarded under the IFFO Global Standard for Responsible Supply

ldquoThe first link in a fully certified aquaculture supply chain is in placerdquo

ldquoSupplies of ldquoIFFO-Assuredrdquo fishmeal and fish oil will shortly be on the marketrdquo

Jorge Mora (right) Operations Director of IFFO presenting the IFFO-Assured certificate to Mario Brescia (left) CEO of TASA at its headquarters in Lima Peru

10 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 11

Me r c a t o r M e d i a o r g a n i s e r o f t h e Offshore Mariculture

Conference 2010 is pleased to announce that the leading i ndus t r y a s soc i a t ions w i l l be suppor t i n g t he 2010 Conference

The European Aquaculture Techno logy amp I nnova t ion Platform (EATiP) the Federation o f Eu ropean Aquacu l tu re Producers (FEAP) the Global

Aquaculture All iance (GAA) and HGK Grupacija akvakul-ture the aquaculture affi l ia-tion of the Croatian Chamber of Economy (and member of FEAP) have all pledged their suppor t to the Conference that wi l l be held from the

June 16-18 2010 at the Hilton Imper ial Hotel Dubrovnik Croatia

Both EATiP (European Aquaculture Technology amp Innovation Platform) and FEAP (Federation of European Aquaculture Producers) are pleased to suppor t the Offshore Mariculture Conference s ince events such as these give fish farmers t h e o p p o r t u -n i t y t o l e a r n from both indus-t r i a l and RTD

exper ts about relevant technological advances to rece ive in formed updates on key research and policy issues and

to be able to network with col leagues from across the sector says Cour tney Hough general secretary of FEAP and EATiP

As wel l as promoting the event to their members and therefore rais ing awareness of the event and its presti-gious speakers it also means that members of any of the above assoc ia t ions wi l l be en t i t l ed to a 10 pe rcen t d i s count o f f the de lega te rate European Aquaculture Society members will also be ent i t led to the discount as the EAS have continued their suppor t f rom the prev ious even t he ld i n A l i c an te i n 2008

The technical conference will be held on the June 16 and 17 June with a visit to a fish farm on the June 18 2010 The Conference Networking Dinner will take place on the evening of Wednesday June 16 2010 at the fabulous Revelin For tress which was bui lt in 1462 to provide protection to the Eastern City Gate Place Over look ing the Old Ci ty Harbour the Revelins terrace offers what are probably the best views of the Old City and its famous Harbour

Mo re in fo rMatio n

Offshore MaricultureTel +44 1622 820622Website wwwoffshoremariculturecom

Industry support strengthens for 2010 OSM Conference

The full programme will be released shortly sessions will include

bull Policy legal framework and key research updates

bull Practical offshore farming operation

bull Spatial planning and moni-toring

bull Spotlight on Croatiabull Ecological monitoring and

future technologiesbull European and International

case studies

iN THE NEWSYour news could feature in our pages

Do you have an news item that you would like to see in our pages Send your press releases to the Production Manager - Nicky BarnesEmail nickybaquafeedcouk

10 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 11

Aqua News

The aquaculture industry today faces a number of challenges that threaten to restrict its development

and profitability

To live in harmony with consumer expec-tations of the highest possible standards of product quality and to do so in a sustainable and environmentally responsible manner necessitates an innovative coordinated and holistic approach within the aquaculture industry

The requirement for sustainability has focused on the composition of the feed being used as one of the key parameters determining the sustainability of production of a particular species particularly the fish-meal and fish oil content and how it relates to overall feed conversion ratios

Further formulation trends are moving towards low fishmeal and oil content in the grower feeds and finishing feeds designed to restore flesh quality parameters such as omega 3 fats and fillet quality that are con-sidered essential for the promotion of the product and its benefits for human health

This strategy addresses to some extent the limited resources of fishmeal and fish oil

Expectations of sustainabilityThe only way to achieve the expecta-

tions of sustainability and replace or supple-ment fishmeal and fish oil in aquafeeds is to exploit alternative protein and lipid sources

The obvious and traditionally used alter-natives are animal and plant protein sources

However in Europe and now increas-ingly in North America consumers pre-fer aquaculture products to have been

fed without the use of animal by-products due in part to recent health scares in the terrestrial animal sec-tor such as bovine spongiform encepha-lopathy (BSE)

The search is on therefore for not only the simple replacement of protein but the provi-sion of functional nutri-tion addressing both the sustainability and flesh quality requirements of the industry while

meeting the nutritional requirement of the animal being cultured

Alternative vegetable protein sources

Over the last two decades aquaculture research has focused on plant protein sources such as grains pulses and oilseeds as they are generally widely available

However the use of soybean meal and other plant based protein sources such as legume seeds oilseed cakes leaf meals leaf protein concentrates and root tuber meals have been limited due to the presence of a wide variety of anti-nutritional substances in these products and their interaction with the physiology of the animal resulting in depressed growth and feed efficiency mortality and abnormal intestinal morphol-ogy and damage

These substances act in different ways by affecting protein utilisation and digestion (eg protease inhibitors tannins lectins) by affecting mineral utilisation (eg phytate gossypol pigments glucosinolates) antivita-mins and other miscellaneous substances (eg phytoestrogens)

The increasing use of plant protein sources is also raising new concerns about possible contamination by mould derived mycotoxins

This risk is enhanced by the global nature and trading of plant protein sourc-es and the as yet little investigated impact of different mycotoxins and their interac-tions in aquaculture species In 2008 the Rapid Alert System for Food and Feed in

Protein sources Alternative protein sources should also show functionality if they are to play a role in modern aquafeedsby Keith Filer PhD research Manager Asia-Pacific biosciences Centre Alltech inc

Figure 1 A comparison of typical essential amino acid (EAA) requirements for fish and the EAA profile of AquateTM

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

juns

wor

th |

Dre

amst

ime

com

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

040

120

08

180

120

08

010

220

08

150

220

08

290

220

08

140

320

08

280

320

08

110

420

08

250

420

08

090

520

08

230

520

08

16 | InternatIonal AquAFeed | March-april 2010

Alltechreg has developed a range of products to address dietary requirements at different

stages of life in aquaculture species These sustainable product solutions are proven to

support profitability in aquaculture operations as well as performance in fish and shrimp

in the Family

Give your fish and shrimp the Alltech Aqua Advantage Contact your Alltech representative today for more information

trade

aquasolutionsalltechcomwwwalltechcom

Ideally suited for fish and shrimp in any stage of life to maintain performance and to support independence from traditional aquaculture diet ingredients

Specifically designed for the juvenile stages of life in fish and shrimp to support good health uniform growth and survival

A natural affordable product created for salmon in all life stages to maintain good health and defense mechanisms against adverse aquatic elements present in the surrounding environment

trade

trade

Aquate Ad for IAF due Feb 19 2010V2indd 1 2192010 115722 AM

Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

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1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

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(UKpound15 per copy)

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Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

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Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

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Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

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EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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If you have a product or service that you want to advertise but we do not have a heading let us know

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 7: March | April 10 - International Aquafeed

ILDEX Vietnam 2010 is ready to offer technology and business solutions for Vietnamrsquos live-

stock dairy meat-processing and aquaculture industries according to NEO - the organizer of ILDEX Vietnam 2010 which will take place on March 25-27 2010 at the New Saigon Exhibition and Convention Center Ho Chi Minh City Vietnam

NCC Exhibition Organizer Co Ltd (NEO) announced the read-iness of ILDEX Vietnam 2010 which will take place on March 25-27 2010 at the New Saigon Exhibit ion and Convention Center in Ho Chi Minh City Vietnam

At the Kick-off Launching of ILDEX Vietnam 2010 Ms Ladda Mongkolchaivivat general manager NCC Exh ib i t ion Organizer (NEO) told industry representative that ldquoILDEX Vietnam 2010 will be the largest livestock exhibition in Vietnam It will be a technology showcase of 125 companies from 22 coun-tries including country pavilion from France Singapore Korea China Shanghai City and the Netherlands

ldquoA number of international governmental and industr ial bodies such as Korean Animal Health Products Association Canadian Swine Association

British Pig Association Vietnam Depar tment of Animal Health V i e t n am Depa r tmen t o f Livestock Production and CCPIT Shanghai have confirmed their par ticipation at this industrial eventrdquo

Wi t h heav y p romot i on campaign and full government suppor t especially from the Ministry of Agricultural and Rural Development of Vietnam NEO believes that ILDEX Vietnam 2010 will attract a lot of traders and buyers from over 46 cities and provinces surrounding Ho Chi Minh City Free shuttle service will be provided for trade visitors from those areas

ILDEX Vietnam 2010 comes in perfect timing as Vietnam live-stock dair y meat-processing and aquaculture industries are searching for new technology and business solutions to move the industry to the next level

Aiming to provide the industry representative with clearer perspective of Vietnam live-stock and aquaculture indus-tries ILDEX invited Professor Hoang Kim Giao director of depar tment of livestock pro-duction Ministry of Agriculture and Rural Development and Mr Nguyen Van Trong vice director-general Aquaculture Research Institute II of Vietnam to give

keynote speeches on ldquoHusbandry in Vietnam ndash Current Status and Development Goalsrdquo and ldquoOver view Aquaculture in Vietnamrdquo respectively

Professor Hoang Kim Giao under lined the impor tant of Vietnam livestock industry

However he said ldquoIt is time for Vietnam to change the struc-ture of its agriculture and rural development Priority should be given to development of live-stock industry including industri-alization of livestock farming and food production management to produce high-quality livestock products that meet international food safety standard and enough to serve rising domestic and export demands Equally impor-tant is to protect the natural envi-ronment for sustainable develop-mentrdquo

Mr Nguyen Van Trong echoed the needs for technology and modern business solutions for Vietnam aquaculture industry Although fish and shrimp have been growing rapidly both have been facing continuous challenges especially rising production costs lowering prices and disease man-agement

ldquoOur aquaculture industr y might be growing a little too fast especially for catfish farming

ldquoThe industr y must ser i -

ously look into sustainable fa rming management Th i s t ime around the emphasis should be on quality rather than quantity to ensure that growth will continue at sus-tainable pace Pangasius for example we are having market problems so while expanding into new markets we need to be cer tain that Vietnamrsquos products meet safety standards and food hygiene required by impor tersrdquo

Apar t from technology and business solutions on the exhi-bition floors ILDEX Vietnam Aquaculture Conference 2010 under the theme lsquothe new dimen-sions for Vietnam Aquaculture Industryrsquo will be a good educa-tion forum for stakeholders along the production chain from pondhatchery operators to technical level personnel to managers feed manufacturers government officers to grasp the issues facing by the industry and develop suitable policies and aquaculture suppliers supporting the industry

Mo re in fo rMatio n

ILDEX Vietnam 2010 and ILDEX Vietnam Aquaculture Conference 2010Mr Lertwat Chanthatarath Senior Sales Manager Tel +66 2 20342614Email lertwatQsncccomWebsite wwwildexcom

ILDEX Vietnam 2010 the solutions for Vietnam sustainable growth

4 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 5

Aqua News

AE2010 is organised by the European Aquaculture Society and hosted by

the Portuguese Marine Sciences Associate Laboratory CIMAR (CIIMAR Por to and CCMAR Faro)

Portugal and PortoAquaculture production in

Por tugal is modest with some 10000 tonnes of production of which more than half is shell-fish

Marine aquaculture in Portugal is mainly centred on the Algarve coast but more recently land-based production of turbot and other marine species has devel-oped on the Atlantic coast

Currently there is renewed interest in developing aqua-culture in Por tugalrsquos outer-most regions notably in the Azores The Portuguese remain Europersquos highest consumers of fish and shellfish with annual per capita consumption of close to 60kg

Por to is Por tugalrsquos second biggest city and the lsquocapital of the nor thrsquo Its historic centre was declared a World Heritage Site by UNESCO in 1996 and it shared the designation European Culture Capital in 2001The city has excellent flight connections with flag and low cost carriers and road connections with Galicia

and especially with Vigo just 90 minutes away

AE2010 will take place at the Centro de Congressos da Alfacircndega ndash the old customs house on the quay of the Douro River in the heart of Porto and just opposite the famous por t wine cellars that are synonymous with this lively city These will form part of the AE2010 social pro-gramme

An extensive conference programme

The thematic sessions take place each morning of AE2010 and include plenary presentations by invited speakers

They pave the way for the parallel technical sessions of con-tributed presentations in both oral and poster format

A vision for the future status of marine aquaculture in Europe effects of a changing environment on biodiversity and physiology of farmed species Changing envi-ronment is at the heart of the European concerns what will be the impacts at short and medium terms For aquaculture pro-ducing specific species not able to maintain a constant body tem-perature (ectotherms) various influences are expected both for species geographical distribu-tion and so adequacy between species and present rearing sites

and impacts on species physi-ology The European aquaculture in mainly producing molluscs and fishes rapidly developed in several countries in taking into account the interdependences between species physiological needs and local conditions in terms of both more or less sheltered areas and environmental factors changes In a situation of rapid environmental changes what are the measures we have to take to follow them maybe to forecast them and to better prepare the aquacul-ture of tomorrow This review proposes to analyse this new sit-uation Presented by Gilles Boeuf President of the French National Museum of Natural History

A license to produce In the light of the new Commission strategy for the development of European aquaculture what type of gover-nance and regulatory framework is needed What should be the roles of public authorities and NGOrsquos in the development of the sector Presented by Javier Ojeda Apromar Spain

Competing claims ndash joint solu-tions How to integrate aquacul-ture with other coastal activities how can aquaculture contribute to the maritime strategy to coastal zone management and to Natura 2000 objectives Presented by Franccedilois Simard IUCN Global Marine Programme

Consumer demands and con-sumption trends What are the demand and consumption trends and what do European con-sumers prefer How important to consumers is the origin and certification of aquatic products produced lsquolocallyrsquo in Europe or impor ted from third coun-tr ies Presented by Philippe Paquotte DG Mare European Commission

Sustainable sea farming How do we develop sustainable sea farming using the ecosystem approach How is aquaculture linked with natural ecosystem processes and with patrimonial cultivation Presented by Joatildeo Gomes Ferreira New University of Lisbon Portugal

AE2010 is now calling for abstracts for presentation in parallel and poster sessions

These sessions are organised in several groups as follows

bull Hatchery production and spec ies d iver s i f i ca t ion including sessions on

bull Finfish hatcher y produc-tion covering all aspects of juvenile production proc-esses and management

bull Shellfish (juvenile) produc-tion including new develop-ments in breeding hatchery production natural spat col-lection

bull Management of aquaculture

The future of marine aquaculture

Aquaculture Europe 2010 to be held in Porto Portugal from October 5-8 2010 puts the focus on the future of marine aquaculture in Europe

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

stocks including reproduc-tion breeding and genetic improvements

bull Species diversification - finfish including the culture of flatfish tuna and other lsquonewrsquo finfish species for future commercial production

bull Species diversification ndash other species including the culture of octopus cuttlefish abalone crabs seaweeds and other lsquonewrsquo species for future commercial production

bull Production in different envi-ronments and produc-tion technologies including sessions on

bull Land-based production - new production technol-ogies including large-scale RAS production in ar id regions and other land based activities

bull Offshore aquaculture of fish and shellfish

bull Aquaculture on Islands ndash specificities of production in outlying areas in tropical and temperate regions including European outer-most regions

Tools for the development of the sector including sessions on

bull The eco-system approach to aquaculture produc-tion including semi inten-siveextensive coastallagoon aquaculture nutrient man-agement and IMTA invasive species predator control nature management and pat-rimonial conservation

bull Space for aquaculture Carrying capacity and Spatial Planning

bull Aquaculture governance and interactions with fish-eries including policy soci-oeconomic and economic research and interactions commonalities and interde-pendence with fisheries

bull Health quality and well-being including sessions on

bull Human health consumer science and quality including

aquaculture percept ion studies biotoxins quality assessment and detection certification

bull Health management of finfish and shellfish Sponsored by Inter vet Schering-Plough Animal Health

bull Novel feeds ingredients and additives Sponsored by Biomin

bull Aquaculture welfare and ethics

The conference will also include a special Student Workshop and naturally a student reception

Science meets industryAquaculture Europe 2010 will

also feature a special interna-tional trade exhibition organised by the European Aquaculture Society Por tuguese Spanish and International companies will present the latest products and ser vices for aquacul-ture A special farmersrsquo day programme will have an emphasis on aquaculture in Por tugal and in Spain and will focus on topics where the latest research findings can have an impact on the sector

It is hoped that the main Por tuguese and Spanish industr y associations for finfish and shel l fish wil l take advantage of the farmersrsquo day to bring their members to Por to and to use the event for their own meetings

Good local and industry support

AE2010 i s f inanc ia l ly suppor ted by the Area Metropolitana do Porto

The gold sponsor of AE2010 is Sorgal SA

E A S i s p r o u d t o announce the AE2010 Gold Sponsorship of Portuguese a q u a f e e d c o m p a n y Aquasoja Aquasoja is the fish feed brand of Sorgal SA Sorgal SA is par t of a

large Por tuguese agro-indus-trial group Sorgal has produced quality feeds for animal farming for more than 50 years and has always conducted its activities focussing on cost-effectiveness animal welfare and feed safety as top priorities

As par t o f i t s Premier Sponsorship of the European Aquaculture Society Inter vet Schering Plough Animal Health will have a strong presence and will sponsor the health manage-ment session

Feed ingredients and addi-t ives specia l i s t B iomin wi l l sponsor the session on Novel feeds ingredients and addi-tives

Those interested in booking exhibition space andor sponsor-ship opportunities should contact Mario Stael at mariostaelscarletbe

EU to showcase FP6 research

The EU Forum ndash a regular feature of Aquaculture Europe events ndash is currently being planned and will look to showcase the results of two of the biggest integrated research projects carried out by the Commission in aquaculture as well as presenting the view of national research funders The EU Forum will also look forward to the final stages of FP7

Just pr ior to AE2010 the Larvanet network will meet in Porto The latest findings of their research will be presented in a special session within the confer-ence A series of short workshops organised by other EU-funded projects and other relevant ini-tiatives will also take place in the exhibition area and specifically targeted towards exhibitors and trade show visitors

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

Kiotechagil the interna-tional supplier of natural high performance feed

additives to enhance growth health and sustainability in agri-culture and aquaculture will have a strong presence at VIV in The Netherlands in April 2010

The company will be high-lighting their new acidifier pHorce which compliments their range of acid products pHorce will provide a concen-trated acid on a micropearl silica carrier and can be used for decontamination of raw materials as an alternative to

c o r r o s i v e liquids and in finished feed at one kgtonne

In addition Kiotechagil will be showcasing a new broad spectrum anti-mycotoxin product which will combine the benefits of their highly effective alumino-silicate Sorbatox with a primary growth yeast cell wall compo-nent high in both beta glucans and mannan oligosaccharides

Mo re in fo rMatio n

Kiotechagil VIV Stand No C066 in Hall 9Tel +44 1189 813333Email infokiotechagilcom

Kiotechagil to show pHorceful presence at VIV

Animal nutrition company Norel launched a new website wwwnorel

es that will provide up-to-date information about its products and the company activities

Founded in 1980 by Mr Enrique Pablos Perez Norel SA has been steadily growing and developing into a well known brand The original meaning of Norel was the Spanish phrase lsquoNO REconocemos Limitesrsquo l i teral ly meaning lsquoWe donrsquot acknowledge limitsrsquo thus dem-onstrating Norelrsquos philosophy The Skyrsquos The Limit or in other words the possibi l i t ies are endless

Norel has become associated with dynamism and innovation (more than five percent of its turnover is invested in Research and Development) and also with quality and performance thanks to our ISO GMP and FAMI-QS certifications

An important feature of Norel is its focus since the beginning on animal nutrition

Originally Norel was a supplier of raw mater ials for animal nutrition milk replacers whey products and protein extracts In 1982 it star ted the production

of spray fat concentrates as well as organic trace minerals (methionates)

Expansion and develop-ment followed and in 1988 Norel opened i ts b io-technology plant in Leon (Spain)

ldquo We t h e n b e c a m e the fi r st b iotechnology company in Spain spe-cialised in animal nutrition In 1990 we star ted the production of by-pass fats for ruminants Today with our flagship Magnapac we are one of the major pro-ducers worldwide Our devel-opment continued with new factories in Egypt and Mexico and specialized units in Spainrdquo says the company in an inter-view with the press

ldquoAn impor tant date in our history is certainly 2001 when Norel SA merged its activities with Nature SA a company spe-cialized in the production of flavours and sweeteners acidi-fiers antioxidants and mould inhibitors

ldquoWe then became known in the market as lsquoNorel amp Naturersquo at that time it was impor tant for us to keep the name Nature

because our act iv it ies and products were different but at the same time very complemen-tary Today we are pleased to introduce our new logo together with the launch of our brand new website wwwnoreles

The new logo of Norel takes into account the histor y by combin ing the two Nrsquos of Norel and Nature Together they resemble the famous Yin Yang symbol and remind us that the two entities now make one The new logo also represents the infinity symbol a reference to the or ig inal meaning of Norel continue to

grow and the possibilities are endless

I t s new webs i t e comes w i th an improved cor po-rate identity and many new features and updates Most impor tant ly i t has added a news section that wil l keep reader s updated on la test developments and activ it ies of Norel worldwide

Mo re in fo rMatio n

Norel SAJesus Aprendiz 19 1 A y BMadrid NC 28007 SpainTel +34 6698 09659Website wwwnorelnaturecom

NOREL announces new web site and new corporate image

8 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 9

Dishman Group o f Ahmedabad I nd i a a lead ing products

and ser vices provider to the pharmaceutical industr y has announced the appointment of Chris Oates to the position of chief operating officer

Mr Oates will repor t to Nick Green president of the Dishman Globa l Contr act Research and Manufactur ing Ser vices (CRAMS) business

M r O a t e s w i l l a s s u m e responsibil ity for Indian and Chinese operations within the company

He wi l l be managing the development operation and improvement of the systems in the two manufacturing sites in India (Bavla and Naroda) and

the new Shanghai facility which is due for completion mid-2010

In addition to the units in Naroda and China Mr Oates wil l be responsible for the nine operating units at Bavla including a large-scale high potency fac i l i ty which wi l l be inaugurated at the end of January The new state-of-the-ar t high potency facil ity will extend Carbogen Amcisrsquo man-ufactur ing capacity to multi-tonne scale and will be the first of its kind to be located in Asia

M r O a t e s rsquo e x p e r i e n c e combines both product ion and operational roles in the contract manufacturing arena He will play a pivotal role in

expanding the companyrsquos core CRAMS business

Mr Oates holds a bach-elorrsquos degree in applied chem-istr y from the University of Huddersfield in the UK His exper t ise is bui lt on more than 20 years working within the pharmaceutical industr y He most recently ser ved as managing director of Uquifa Mexico His previous expe-r ience includes six year s at Avecia Pharmaceuticals in the UK where he held the position of operations manager and as the position of senior pro-duction manager for Syngenta (Agrochemicals)

ldquo I am looking forward to taking up this new challenge and am very excited by what I

believe will be a very successful future for the companyrdquo said Mr Oates

ldquoDishman can provide the full continuum of ser vices from process RampD to large-scale commercial manufacturing in multiple locations around the globe The company is ideally p l aced to bu i ld upon i t s success to date and become the industr y leading CRAMS par tnerrdquo

Mo re in fo rMatio n

Dishman Europe LimitedSuite 4 De Walden Court 85 New Cavendish Street London W1W 6XDUnited KingdomTel +44 2073 230608 Fax +44 2073 230609Website wwwdishmangroupcom

Dishman appoints Chris Oates as chief operating officerNew role will oversee Dishman Indian and Chinese Facilities

Sponsorship of a keynote session at Aquaculture 2010 confirms Biominrsquos

firm commitment and keen focus on this dynamic growth sector

Biomin embraces the new year with a bold plunge in aqua-culture

Fo r the f i r s t t ime the company wil l be par tner ing the World Aquaculture Society (WAS) with the sponsorship of the Aquafeeds Session at Aquaculture 2010

To be held in San Diego from March 1-5 2010 Aquaculture 2010 is the largest event for the global aquaculture industry Hosted every year by the WAS the global aquaculture event is expected to draw some 4000 participants from over 80 coun-tr ies including high profi le

attendees who form the core of decision-makers and innova-tors in the aquatic business

Biomin is proud to be a session sponsor at the worldrsquos largest aquaculture event Held on March 4 2010 the session on lsquoAquafeedsrsquo will see exper t speakers from com-mercial practice and academia share their i n s i gh t s on ma jor issues in aquafeeds formulation and alter-nat ives to fishmeal and protein replace-ment strategies for a variety of aquatic species

Years of dedicated applied research and investments have strengthened Biominrsquos drive and reputation in providing effective natural-based feed solutions for the aquaculture and aquafeed

business throughout the world A core focus on fundamental applied research forms the basis of Biominrsquos portfolio of science-based aquaculture product solu-tions Biominreg AquaSpecials which includes nutraceuticals probiotics natural growth pro-

moters mycotoxin deactiva-tors and functional feed addi-tives

Dr Pedro Encarnaccedilatildeo Biominrsquos aquacul ture spec ia l i s t says ldquoBiomin is a research-focused and -driven company

ldquoWe have translated our long-

standing competence in animal nutrition for terrestrial species into aquaculture and aquafeed research Our Aquatic Centre for Applied Animals (AQUACAN) in Thailand actively conducts feeding trials for many aquatic species from which new findings

have been translated into innovative and practical solutions in aquafeedsrdquo

Biomin will also par-ticipate as a sponsor at the Australas ian Aquacu l ture 2010

another WAS event to be held in Hobar t Australia in May 2010 and at the Aquaculture Europe that will take place in Por to Por tugal in October 2010 both of which are key events for aquaculture in the coming year

A bold step for BIOMIN aquacultureBiomin proud to be session sponsor at the worldrsquos largest aquaculture event

8 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 9

Aqua News

Tecnoloacutegica de Alimentos SA (TASA) of Peru is the first fishmeal and fish

oil producer to achieve cer ti-fication under the new IFFO Global Standard for Responsible Supply

The cer tification has been awarded to the Callao Nor te plant of TASA the worldrsquos largest fishmeal producer Earlier this week Jorge Mora Operations Director of IFFO presented the

IFFO-Assured certificate to Mario Brescia CEO of TASA at its head-quarters in Lima Peru

IFFO has been developing its RS Standard since early 2008 with the help of a Technical Advisory Committee comprising repre-sentatives from the whole aqua-culture value chain including fish farmers feed producers and retailers and also NGOs

This business-to-business inde-pendently-audited programme will enable IFFO members to demonstrate to their customers that they are offer ing pure marine ingredients manufactured from responsibly sourced and traceable raw materials Whole fish used must come from fish-eries managed under the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

ldquoSince October 2009 when we launched the programme we have had applications from 15 manufacturing companies in four major producing countries sourcing from 10 fisheriesrdquo says IFFO director general Jonathan Shepherd

ldquoWe anticipate that there will be substantial supplies of IFFO-Assured fishmeal and fish oil on the market within months and that up to one third of global

p r o d u c t i o n could be certi-fied by the end of 2010rdquo

ldquoWith th i s p r o g r a m m e IFFO has put in place the first link in a fully certified supply chain for aqua-culture and for land animals such as pigs and poul tr y

It will help the industry demon-strate its commitment to respon-sible manufacture of fishmeal and fish oil as well as to the FAO Code of Conductrdquo said Dr Shepherd

Applicant factories which can demonstrate that they are ldquooper-ating a well managed system that ensures their marine raw mate-rials are responsibly sourced and processed to the IFFO Standardrdquo will be awarded certification

They will be able to offer IFFO-Assured fishmeal and fish oil and have the right to display the RS logo An up-to-date list of cur-rently certified fishmeal plants will be posted on the IFFO web site alongside the full Standard

Characteristics of the IFFO Global Standard for Responsible Supply (RS)

bull Standards set by a multi-stake-holder Technical Advisory

Committee including pro-ducers traders fish farmers feed manufacturers proc-essors retailers standard setting organisations and environmental NGOs The Assessment Management and Certification Process are contracted to Global Trust

bull A programme independ-ently administered by an ISO 651996 accredited certifica-tion body which is a member of the International Accreditation Forum (IFA)

bull I t d e m o n s t r a t e s responsible sourcing o f t r aceable r aw material and respon-sible production to ensure feed safety

bull A business-to-busi-ness tool designed to enable fishmeal and fish oil producers to demonstrate the integr i ty o f the i r products and their processes to their cus-tomers in the value-chain rather than a consumer eco-label

bull Whole fish used must come from fisheries that have been independently assessed and meet the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

bull Exclusion of illegal unre-por ted and unregulated (IUU) fish

bull The factor y must have attained Cer tification to the Internat ional Feed Ingredients Standard (IFIS) of the International Feed Safety Alliance (IFSA) or equiva-lent as proof of responsible manufacturing and the appli-cant must be in possession

of all relevant permits and licenses for the production and sale of fishmeal and fish oil products

bull This programme is com-patible with ISO 651996 (General requirements for bodies operating product cer tification systems) and with EN45011

bull Recogn i ses other cer-t i f i c a t ion p rog r ammes w h i c h h av e d e m o n -

strated equivalency and which are accepted within the industry - eg Marine Stewardship Council (MSC) for the fishery certification element only

The IFFO programme will continue to be developed in 2010 addressing further aspects of responsible supply

Mo re in fo rMatio n

Andrew JacksonTechnical Director IFFO 2 College YardLower Dagnall Street St Albans Hertfordshire AL3 4PAUnited KingdomTel +44 7808 783488 Email ajacksoniffonetWebiste wwwiffonet

First certification awarded under the IFFO Global Standard for Responsible Supply

ldquoThe first link in a fully certified aquaculture supply chain is in placerdquo

ldquoSupplies of ldquoIFFO-Assuredrdquo fishmeal and fish oil will shortly be on the marketrdquo

Jorge Mora (right) Operations Director of IFFO presenting the IFFO-Assured certificate to Mario Brescia (left) CEO of TASA at its headquarters in Lima Peru

10 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 11

Me r c a t o r M e d i a o r g a n i s e r o f t h e Offshore Mariculture

Conference 2010 is pleased to announce that the leading i ndus t r y a s soc i a t ions w i l l be suppor t i n g t he 2010 Conference

The European Aquaculture Techno logy amp I nnova t ion Platform (EATiP) the Federation o f Eu ropean Aquacu l tu re Producers (FEAP) the Global

Aquaculture All iance (GAA) and HGK Grupacija akvakul-ture the aquaculture affi l ia-tion of the Croatian Chamber of Economy (and member of FEAP) have all pledged their suppor t to the Conference that wi l l be held from the

June 16-18 2010 at the Hilton Imper ial Hotel Dubrovnik Croatia

Both EATiP (European Aquaculture Technology amp Innovation Platform) and FEAP (Federation of European Aquaculture Producers) are pleased to suppor t the Offshore Mariculture Conference s ince events such as these give fish farmers t h e o p p o r t u -n i t y t o l e a r n from both indus-t r i a l and RTD

exper ts about relevant technological advances to rece ive in formed updates on key research and policy issues and

to be able to network with col leagues from across the sector says Cour tney Hough general secretary of FEAP and EATiP

As wel l as promoting the event to their members and therefore rais ing awareness of the event and its presti-gious speakers it also means that members of any of the above assoc ia t ions wi l l be en t i t l ed to a 10 pe rcen t d i s count o f f the de lega te rate European Aquaculture Society members will also be ent i t led to the discount as the EAS have continued their suppor t f rom the prev ious even t he ld i n A l i c an te i n 2008

The technical conference will be held on the June 16 and 17 June with a visit to a fish farm on the June 18 2010 The Conference Networking Dinner will take place on the evening of Wednesday June 16 2010 at the fabulous Revelin For tress which was bui lt in 1462 to provide protection to the Eastern City Gate Place Over look ing the Old Ci ty Harbour the Revelins terrace offers what are probably the best views of the Old City and its famous Harbour

Mo re in fo rMatio n

Offshore MaricultureTel +44 1622 820622Website wwwoffshoremariculturecom

Industry support strengthens for 2010 OSM Conference

The full programme will be released shortly sessions will include

bull Policy legal framework and key research updates

bull Practical offshore farming operation

bull Spatial planning and moni-toring

bull Spotlight on Croatiabull Ecological monitoring and

future technologiesbull European and International

case studies

iN THE NEWSYour news could feature in our pages

Do you have an news item that you would like to see in our pages Send your press releases to the Production Manager - Nicky BarnesEmail nickybaquafeedcouk

10 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 11

Aqua News

The aquaculture industry today faces a number of challenges that threaten to restrict its development

and profitability

To live in harmony with consumer expec-tations of the highest possible standards of product quality and to do so in a sustainable and environmentally responsible manner necessitates an innovative coordinated and holistic approach within the aquaculture industry

The requirement for sustainability has focused on the composition of the feed being used as one of the key parameters determining the sustainability of production of a particular species particularly the fish-meal and fish oil content and how it relates to overall feed conversion ratios

Further formulation trends are moving towards low fishmeal and oil content in the grower feeds and finishing feeds designed to restore flesh quality parameters such as omega 3 fats and fillet quality that are con-sidered essential for the promotion of the product and its benefits for human health

This strategy addresses to some extent the limited resources of fishmeal and fish oil

Expectations of sustainabilityThe only way to achieve the expecta-

tions of sustainability and replace or supple-ment fishmeal and fish oil in aquafeeds is to exploit alternative protein and lipid sources

The obvious and traditionally used alter-natives are animal and plant protein sources

However in Europe and now increas-ingly in North America consumers pre-fer aquaculture products to have been

fed without the use of animal by-products due in part to recent health scares in the terrestrial animal sec-tor such as bovine spongiform encepha-lopathy (BSE)

The search is on therefore for not only the simple replacement of protein but the provi-sion of functional nutri-tion addressing both the sustainability and flesh quality requirements of the industry while

meeting the nutritional requirement of the animal being cultured

Alternative vegetable protein sources

Over the last two decades aquaculture research has focused on plant protein sources such as grains pulses and oilseeds as they are generally widely available

However the use of soybean meal and other plant based protein sources such as legume seeds oilseed cakes leaf meals leaf protein concentrates and root tuber meals have been limited due to the presence of a wide variety of anti-nutritional substances in these products and their interaction with the physiology of the animal resulting in depressed growth and feed efficiency mortality and abnormal intestinal morphol-ogy and damage

These substances act in different ways by affecting protein utilisation and digestion (eg protease inhibitors tannins lectins) by affecting mineral utilisation (eg phytate gossypol pigments glucosinolates) antivita-mins and other miscellaneous substances (eg phytoestrogens)

The increasing use of plant protein sources is also raising new concerns about possible contamination by mould derived mycotoxins

This risk is enhanced by the global nature and trading of plant protein sourc-es and the as yet little investigated impact of different mycotoxins and their interac-tions in aquaculture species In 2008 the Rapid Alert System for Food and Feed in

Protein sources Alternative protein sources should also show functionality if they are to play a role in modern aquafeedsby Keith Filer PhD research Manager Asia-Pacific biosciences Centre Alltech inc

Figure 1 A comparison of typical essential amino acid (EAA) requirements for fish and the EAA profile of AquateTM

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

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the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

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12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

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have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

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retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

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conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

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Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

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16 | InternatIonal AquAFeed | March-april 2010

Alltechreg has developed a range of products to address dietary requirements at different

stages of life in aquaculture species These sustainable product solutions are proven to

support profitability in aquaculture operations as well as performance in fish and shrimp

in the Family

Give your fish and shrimp the Alltech Aqua Advantage Contact your Alltech representative today for more information

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aquasolutionsalltechcomwwwalltechcom

Ideally suited for fish and shrimp in any stage of life to maintain performance and to support independence from traditional aquaculture diet ingredients

Specifically designed for the juvenile stages of life in fish and shrimp to support good health uniform growth and survival

A natural affordable product created for salmon in all life stages to maintain good health and defense mechanisms against adverse aquatic elements present in the surrounding environment

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Aquate Ad for IAF due Feb 19 2010V2indd 1 2192010 115722 AM

Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

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Dry with 20-50 less energy

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of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

8 page full colour document - 100 copies = UKpound489 (please add UKpound15 for every additional 100 copies)

Delivery charged on a case by case basis

For more details please visit wwwaquafeedcoukreprints

1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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Quick Links

Contact the teamMore About Us

IAF1001

Buy a paper copy ofthis issue

(UKpound15 per copy)

Subscribe to IAFSubscribers to IAF receive 5 issues

per year plus a copy of theInternational Aquafeed Directory amp

Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

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Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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To place a classified advert in our next issue and take

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WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

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BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 8: March | April 10 - International Aquafeed

AE2010 is organised by the European Aquaculture Society and hosted by

the Portuguese Marine Sciences Associate Laboratory CIMAR (CIIMAR Por to and CCMAR Faro)

Portugal and PortoAquaculture production in

Por tugal is modest with some 10000 tonnes of production of which more than half is shell-fish

Marine aquaculture in Portugal is mainly centred on the Algarve coast but more recently land-based production of turbot and other marine species has devel-oped on the Atlantic coast

Currently there is renewed interest in developing aqua-culture in Por tugalrsquos outer-most regions notably in the Azores The Portuguese remain Europersquos highest consumers of fish and shellfish with annual per capita consumption of close to 60kg

Por to is Por tugalrsquos second biggest city and the lsquocapital of the nor thrsquo Its historic centre was declared a World Heritage Site by UNESCO in 1996 and it shared the designation European Culture Capital in 2001The city has excellent flight connections with flag and low cost carriers and road connections with Galicia

and especially with Vigo just 90 minutes away

AE2010 will take place at the Centro de Congressos da Alfacircndega ndash the old customs house on the quay of the Douro River in the heart of Porto and just opposite the famous por t wine cellars that are synonymous with this lively city These will form part of the AE2010 social pro-gramme

An extensive conference programme

The thematic sessions take place each morning of AE2010 and include plenary presentations by invited speakers

They pave the way for the parallel technical sessions of con-tributed presentations in both oral and poster format

A vision for the future status of marine aquaculture in Europe effects of a changing environment on biodiversity and physiology of farmed species Changing envi-ronment is at the heart of the European concerns what will be the impacts at short and medium terms For aquaculture pro-ducing specific species not able to maintain a constant body tem-perature (ectotherms) various influences are expected both for species geographical distribu-tion and so adequacy between species and present rearing sites

and impacts on species physi-ology The European aquaculture in mainly producing molluscs and fishes rapidly developed in several countries in taking into account the interdependences between species physiological needs and local conditions in terms of both more or less sheltered areas and environmental factors changes In a situation of rapid environmental changes what are the measures we have to take to follow them maybe to forecast them and to better prepare the aquacul-ture of tomorrow This review proposes to analyse this new sit-uation Presented by Gilles Boeuf President of the French National Museum of Natural History

A license to produce In the light of the new Commission strategy for the development of European aquaculture what type of gover-nance and regulatory framework is needed What should be the roles of public authorities and NGOrsquos in the development of the sector Presented by Javier Ojeda Apromar Spain

Competing claims ndash joint solu-tions How to integrate aquacul-ture with other coastal activities how can aquaculture contribute to the maritime strategy to coastal zone management and to Natura 2000 objectives Presented by Franccedilois Simard IUCN Global Marine Programme

Consumer demands and con-sumption trends What are the demand and consumption trends and what do European con-sumers prefer How important to consumers is the origin and certification of aquatic products produced lsquolocallyrsquo in Europe or impor ted from third coun-tr ies Presented by Philippe Paquotte DG Mare European Commission

Sustainable sea farming How do we develop sustainable sea farming using the ecosystem approach How is aquaculture linked with natural ecosystem processes and with patrimonial cultivation Presented by Joatildeo Gomes Ferreira New University of Lisbon Portugal

AE2010 is now calling for abstracts for presentation in parallel and poster sessions

These sessions are organised in several groups as follows

bull Hatchery production and spec ies d iver s i f i ca t ion including sessions on

bull Finfish hatcher y produc-tion covering all aspects of juvenile production proc-esses and management

bull Shellfish (juvenile) produc-tion including new develop-ments in breeding hatchery production natural spat col-lection

bull Management of aquaculture

The future of marine aquaculture

Aquaculture Europe 2010 to be held in Porto Portugal from October 5-8 2010 puts the focus on the future of marine aquaculture in Europe

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

stocks including reproduc-tion breeding and genetic improvements

bull Species diversification - finfish including the culture of flatfish tuna and other lsquonewrsquo finfish species for future commercial production

bull Species diversification ndash other species including the culture of octopus cuttlefish abalone crabs seaweeds and other lsquonewrsquo species for future commercial production

bull Production in different envi-ronments and produc-tion technologies including sessions on

bull Land-based production - new production technol-ogies including large-scale RAS production in ar id regions and other land based activities

bull Offshore aquaculture of fish and shellfish

bull Aquaculture on Islands ndash specificities of production in outlying areas in tropical and temperate regions including European outer-most regions

Tools for the development of the sector including sessions on

bull The eco-system approach to aquaculture produc-tion including semi inten-siveextensive coastallagoon aquaculture nutrient man-agement and IMTA invasive species predator control nature management and pat-rimonial conservation

bull Space for aquaculture Carrying capacity and Spatial Planning

bull Aquaculture governance and interactions with fish-eries including policy soci-oeconomic and economic research and interactions commonalities and interde-pendence with fisheries

bull Health quality and well-being including sessions on

bull Human health consumer science and quality including

aquaculture percept ion studies biotoxins quality assessment and detection certification

bull Health management of finfish and shellfish Sponsored by Inter vet Schering-Plough Animal Health

bull Novel feeds ingredients and additives Sponsored by Biomin

bull Aquaculture welfare and ethics

The conference will also include a special Student Workshop and naturally a student reception

Science meets industryAquaculture Europe 2010 will

also feature a special interna-tional trade exhibition organised by the European Aquaculture Society Por tuguese Spanish and International companies will present the latest products and ser vices for aquacul-ture A special farmersrsquo day programme will have an emphasis on aquaculture in Por tugal and in Spain and will focus on topics where the latest research findings can have an impact on the sector

It is hoped that the main Por tuguese and Spanish industr y associations for finfish and shel l fish wil l take advantage of the farmersrsquo day to bring their members to Por to and to use the event for their own meetings

Good local and industry support

AE2010 i s f inanc ia l ly suppor ted by the Area Metropolitana do Porto

The gold sponsor of AE2010 is Sorgal SA

E A S i s p r o u d t o announce the AE2010 Gold Sponsorship of Portuguese a q u a f e e d c o m p a n y Aquasoja Aquasoja is the fish feed brand of Sorgal SA Sorgal SA is par t of a

large Por tuguese agro-indus-trial group Sorgal has produced quality feeds for animal farming for more than 50 years and has always conducted its activities focussing on cost-effectiveness animal welfare and feed safety as top priorities

As par t o f i t s Premier Sponsorship of the European Aquaculture Society Inter vet Schering Plough Animal Health will have a strong presence and will sponsor the health manage-ment session

Feed ingredients and addi-t ives specia l i s t B iomin wi l l sponsor the session on Novel feeds ingredients and addi-tives

Those interested in booking exhibition space andor sponsor-ship opportunities should contact Mario Stael at mariostaelscarletbe

EU to showcase FP6 research

The EU Forum ndash a regular feature of Aquaculture Europe events ndash is currently being planned and will look to showcase the results of two of the biggest integrated research projects carried out by the Commission in aquaculture as well as presenting the view of national research funders The EU Forum will also look forward to the final stages of FP7

Just pr ior to AE2010 the Larvanet network will meet in Porto The latest findings of their research will be presented in a special session within the confer-ence A series of short workshops organised by other EU-funded projects and other relevant ini-tiatives will also take place in the exhibition area and specifically targeted towards exhibitors and trade show visitors

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

Kiotechagil the interna-tional supplier of natural high performance feed

additives to enhance growth health and sustainability in agri-culture and aquaculture will have a strong presence at VIV in The Netherlands in April 2010

The company will be high-lighting their new acidifier pHorce which compliments their range of acid products pHorce will provide a concen-trated acid on a micropearl silica carrier and can be used for decontamination of raw materials as an alternative to

c o r r o s i v e liquids and in finished feed at one kgtonne

In addition Kiotechagil will be showcasing a new broad spectrum anti-mycotoxin product which will combine the benefits of their highly effective alumino-silicate Sorbatox with a primary growth yeast cell wall compo-nent high in both beta glucans and mannan oligosaccharides

Mo re in fo rMatio n

Kiotechagil VIV Stand No C066 in Hall 9Tel +44 1189 813333Email infokiotechagilcom

Kiotechagil to show pHorceful presence at VIV

Animal nutrition company Norel launched a new website wwwnorel

es that will provide up-to-date information about its products and the company activities

Founded in 1980 by Mr Enrique Pablos Perez Norel SA has been steadily growing and developing into a well known brand The original meaning of Norel was the Spanish phrase lsquoNO REconocemos Limitesrsquo l i teral ly meaning lsquoWe donrsquot acknowledge limitsrsquo thus dem-onstrating Norelrsquos philosophy The Skyrsquos The Limit or in other words the possibi l i t ies are endless

Norel has become associated with dynamism and innovation (more than five percent of its turnover is invested in Research and Development) and also with quality and performance thanks to our ISO GMP and FAMI-QS certifications

An important feature of Norel is its focus since the beginning on animal nutrition

Originally Norel was a supplier of raw mater ials for animal nutrition milk replacers whey products and protein extracts In 1982 it star ted the production

of spray fat concentrates as well as organic trace minerals (methionates)

Expansion and develop-ment followed and in 1988 Norel opened i ts b io-technology plant in Leon (Spain)

ldquo We t h e n b e c a m e the fi r st b iotechnology company in Spain spe-cialised in animal nutrition In 1990 we star ted the production of by-pass fats for ruminants Today with our flagship Magnapac we are one of the major pro-ducers worldwide Our devel-opment continued with new factories in Egypt and Mexico and specialized units in Spainrdquo says the company in an inter-view with the press

ldquoAn impor tant date in our history is certainly 2001 when Norel SA merged its activities with Nature SA a company spe-cialized in the production of flavours and sweeteners acidi-fiers antioxidants and mould inhibitors

ldquoWe then became known in the market as lsquoNorel amp Naturersquo at that time it was impor tant for us to keep the name Nature

because our act iv it ies and products were different but at the same time very complemen-tary Today we are pleased to introduce our new logo together with the launch of our brand new website wwwnoreles

The new logo of Norel takes into account the histor y by combin ing the two Nrsquos of Norel and Nature Together they resemble the famous Yin Yang symbol and remind us that the two entities now make one The new logo also represents the infinity symbol a reference to the or ig inal meaning of Norel continue to

grow and the possibilities are endless

I t s new webs i t e comes w i th an improved cor po-rate identity and many new features and updates Most impor tant ly i t has added a news section that wil l keep reader s updated on la test developments and activ it ies of Norel worldwide

Mo re in fo rMatio n

Norel SAJesus Aprendiz 19 1 A y BMadrid NC 28007 SpainTel +34 6698 09659Website wwwnorelnaturecom

NOREL announces new web site and new corporate image

8 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 9

Dishman Group o f Ahmedabad I nd i a a lead ing products

and ser vices provider to the pharmaceutical industr y has announced the appointment of Chris Oates to the position of chief operating officer

Mr Oates will repor t to Nick Green president of the Dishman Globa l Contr act Research and Manufactur ing Ser vices (CRAMS) business

M r O a t e s w i l l a s s u m e responsibil ity for Indian and Chinese operations within the company

He wi l l be managing the development operation and improvement of the systems in the two manufacturing sites in India (Bavla and Naroda) and

the new Shanghai facility which is due for completion mid-2010

In addition to the units in Naroda and China Mr Oates wil l be responsible for the nine operating units at Bavla including a large-scale high potency fac i l i ty which wi l l be inaugurated at the end of January The new state-of-the-ar t high potency facil ity will extend Carbogen Amcisrsquo man-ufactur ing capacity to multi-tonne scale and will be the first of its kind to be located in Asia

M r O a t e s rsquo e x p e r i e n c e combines both product ion and operational roles in the contract manufacturing arena He will play a pivotal role in

expanding the companyrsquos core CRAMS business

Mr Oates holds a bach-elorrsquos degree in applied chem-istr y from the University of Huddersfield in the UK His exper t ise is bui lt on more than 20 years working within the pharmaceutical industr y He most recently ser ved as managing director of Uquifa Mexico His previous expe-r ience includes six year s at Avecia Pharmaceuticals in the UK where he held the position of operations manager and as the position of senior pro-duction manager for Syngenta (Agrochemicals)

ldquo I am looking forward to taking up this new challenge and am very excited by what I

believe will be a very successful future for the companyrdquo said Mr Oates

ldquoDishman can provide the full continuum of ser vices from process RampD to large-scale commercial manufacturing in multiple locations around the globe The company is ideally p l aced to bu i ld upon i t s success to date and become the industr y leading CRAMS par tnerrdquo

Mo re in fo rMatio n

Dishman Europe LimitedSuite 4 De Walden Court 85 New Cavendish Street London W1W 6XDUnited KingdomTel +44 2073 230608 Fax +44 2073 230609Website wwwdishmangroupcom

Dishman appoints Chris Oates as chief operating officerNew role will oversee Dishman Indian and Chinese Facilities

Sponsorship of a keynote session at Aquaculture 2010 confirms Biominrsquos

firm commitment and keen focus on this dynamic growth sector

Biomin embraces the new year with a bold plunge in aqua-culture

Fo r the f i r s t t ime the company wil l be par tner ing the World Aquaculture Society (WAS) with the sponsorship of the Aquafeeds Session at Aquaculture 2010

To be held in San Diego from March 1-5 2010 Aquaculture 2010 is the largest event for the global aquaculture industry Hosted every year by the WAS the global aquaculture event is expected to draw some 4000 participants from over 80 coun-tr ies including high profi le

attendees who form the core of decision-makers and innova-tors in the aquatic business

Biomin is proud to be a session sponsor at the worldrsquos largest aquaculture event Held on March 4 2010 the session on lsquoAquafeedsrsquo will see exper t speakers from com-mercial practice and academia share their i n s i gh t s on ma jor issues in aquafeeds formulation and alter-nat ives to fishmeal and protein replace-ment strategies for a variety of aquatic species

Years of dedicated applied research and investments have strengthened Biominrsquos drive and reputation in providing effective natural-based feed solutions for the aquaculture and aquafeed

business throughout the world A core focus on fundamental applied research forms the basis of Biominrsquos portfolio of science-based aquaculture product solu-tions Biominreg AquaSpecials which includes nutraceuticals probiotics natural growth pro-

moters mycotoxin deactiva-tors and functional feed addi-tives

Dr Pedro Encarnaccedilatildeo Biominrsquos aquacul ture spec ia l i s t says ldquoBiomin is a research-focused and -driven company

ldquoWe have translated our long-

standing competence in animal nutrition for terrestrial species into aquaculture and aquafeed research Our Aquatic Centre for Applied Animals (AQUACAN) in Thailand actively conducts feeding trials for many aquatic species from which new findings

have been translated into innovative and practical solutions in aquafeedsrdquo

Biomin will also par-ticipate as a sponsor at the Australas ian Aquacu l ture 2010

another WAS event to be held in Hobar t Australia in May 2010 and at the Aquaculture Europe that will take place in Por to Por tugal in October 2010 both of which are key events for aquaculture in the coming year

A bold step for BIOMIN aquacultureBiomin proud to be session sponsor at the worldrsquos largest aquaculture event

8 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 9

Aqua News

Tecnoloacutegica de Alimentos SA (TASA) of Peru is the first fishmeal and fish

oil producer to achieve cer ti-fication under the new IFFO Global Standard for Responsible Supply

The cer tification has been awarded to the Callao Nor te plant of TASA the worldrsquos largest fishmeal producer Earlier this week Jorge Mora Operations Director of IFFO presented the

IFFO-Assured certificate to Mario Brescia CEO of TASA at its head-quarters in Lima Peru

IFFO has been developing its RS Standard since early 2008 with the help of a Technical Advisory Committee comprising repre-sentatives from the whole aqua-culture value chain including fish farmers feed producers and retailers and also NGOs

This business-to-business inde-pendently-audited programme will enable IFFO members to demonstrate to their customers that they are offer ing pure marine ingredients manufactured from responsibly sourced and traceable raw materials Whole fish used must come from fish-eries managed under the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

ldquoSince October 2009 when we launched the programme we have had applications from 15 manufacturing companies in four major producing countries sourcing from 10 fisheriesrdquo says IFFO director general Jonathan Shepherd

ldquoWe anticipate that there will be substantial supplies of IFFO-Assured fishmeal and fish oil on the market within months and that up to one third of global

p r o d u c t i o n could be certi-fied by the end of 2010rdquo

ldquoWith th i s p r o g r a m m e IFFO has put in place the first link in a fully certified supply chain for aqua-culture and for land animals such as pigs and poul tr y

It will help the industry demon-strate its commitment to respon-sible manufacture of fishmeal and fish oil as well as to the FAO Code of Conductrdquo said Dr Shepherd

Applicant factories which can demonstrate that they are ldquooper-ating a well managed system that ensures their marine raw mate-rials are responsibly sourced and processed to the IFFO Standardrdquo will be awarded certification

They will be able to offer IFFO-Assured fishmeal and fish oil and have the right to display the RS logo An up-to-date list of cur-rently certified fishmeal plants will be posted on the IFFO web site alongside the full Standard

Characteristics of the IFFO Global Standard for Responsible Supply (RS)

bull Standards set by a multi-stake-holder Technical Advisory

Committee including pro-ducers traders fish farmers feed manufacturers proc-essors retailers standard setting organisations and environmental NGOs The Assessment Management and Certification Process are contracted to Global Trust

bull A programme independ-ently administered by an ISO 651996 accredited certifica-tion body which is a member of the International Accreditation Forum (IFA)

bull I t d e m o n s t r a t e s responsible sourcing o f t r aceable r aw material and respon-sible production to ensure feed safety

bull A business-to-busi-ness tool designed to enable fishmeal and fish oil producers to demonstrate the integr i ty o f the i r products and their processes to their cus-tomers in the value-chain rather than a consumer eco-label

bull Whole fish used must come from fisheries that have been independently assessed and meet the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

bull Exclusion of illegal unre-por ted and unregulated (IUU) fish

bull The factor y must have attained Cer tification to the Internat ional Feed Ingredients Standard (IFIS) of the International Feed Safety Alliance (IFSA) or equiva-lent as proof of responsible manufacturing and the appli-cant must be in possession

of all relevant permits and licenses for the production and sale of fishmeal and fish oil products

bull This programme is com-patible with ISO 651996 (General requirements for bodies operating product cer tification systems) and with EN45011

bull Recogn i ses other cer-t i f i c a t ion p rog r ammes w h i c h h av e d e m o n -

strated equivalency and which are accepted within the industry - eg Marine Stewardship Council (MSC) for the fishery certification element only

The IFFO programme will continue to be developed in 2010 addressing further aspects of responsible supply

Mo re in fo rMatio n

Andrew JacksonTechnical Director IFFO 2 College YardLower Dagnall Street St Albans Hertfordshire AL3 4PAUnited KingdomTel +44 7808 783488 Email ajacksoniffonetWebiste wwwiffonet

First certification awarded under the IFFO Global Standard for Responsible Supply

ldquoThe first link in a fully certified aquaculture supply chain is in placerdquo

ldquoSupplies of ldquoIFFO-Assuredrdquo fishmeal and fish oil will shortly be on the marketrdquo

Jorge Mora (right) Operations Director of IFFO presenting the IFFO-Assured certificate to Mario Brescia (left) CEO of TASA at its headquarters in Lima Peru

10 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 11

Me r c a t o r M e d i a o r g a n i s e r o f t h e Offshore Mariculture

Conference 2010 is pleased to announce that the leading i ndus t r y a s soc i a t ions w i l l be suppor t i n g t he 2010 Conference

The European Aquaculture Techno logy amp I nnova t ion Platform (EATiP) the Federation o f Eu ropean Aquacu l tu re Producers (FEAP) the Global

Aquaculture All iance (GAA) and HGK Grupacija akvakul-ture the aquaculture affi l ia-tion of the Croatian Chamber of Economy (and member of FEAP) have all pledged their suppor t to the Conference that wi l l be held from the

June 16-18 2010 at the Hilton Imper ial Hotel Dubrovnik Croatia

Both EATiP (European Aquaculture Technology amp Innovation Platform) and FEAP (Federation of European Aquaculture Producers) are pleased to suppor t the Offshore Mariculture Conference s ince events such as these give fish farmers t h e o p p o r t u -n i t y t o l e a r n from both indus-t r i a l and RTD

exper ts about relevant technological advances to rece ive in formed updates on key research and policy issues and

to be able to network with col leagues from across the sector says Cour tney Hough general secretary of FEAP and EATiP

As wel l as promoting the event to their members and therefore rais ing awareness of the event and its presti-gious speakers it also means that members of any of the above assoc ia t ions wi l l be en t i t l ed to a 10 pe rcen t d i s count o f f the de lega te rate European Aquaculture Society members will also be ent i t led to the discount as the EAS have continued their suppor t f rom the prev ious even t he ld i n A l i c an te i n 2008

The technical conference will be held on the June 16 and 17 June with a visit to a fish farm on the June 18 2010 The Conference Networking Dinner will take place on the evening of Wednesday June 16 2010 at the fabulous Revelin For tress which was bui lt in 1462 to provide protection to the Eastern City Gate Place Over look ing the Old Ci ty Harbour the Revelins terrace offers what are probably the best views of the Old City and its famous Harbour

Mo re in fo rMatio n

Offshore MaricultureTel +44 1622 820622Website wwwoffshoremariculturecom

Industry support strengthens for 2010 OSM Conference

The full programme will be released shortly sessions will include

bull Policy legal framework and key research updates

bull Practical offshore farming operation

bull Spatial planning and moni-toring

bull Spotlight on Croatiabull Ecological monitoring and

future technologiesbull European and International

case studies

iN THE NEWSYour news could feature in our pages

Do you have an news item that you would like to see in our pages Send your press releases to the Production Manager - Nicky BarnesEmail nickybaquafeedcouk

10 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 11

Aqua News

The aquaculture industry today faces a number of challenges that threaten to restrict its development

and profitability

To live in harmony with consumer expec-tations of the highest possible standards of product quality and to do so in a sustainable and environmentally responsible manner necessitates an innovative coordinated and holistic approach within the aquaculture industry

The requirement for sustainability has focused on the composition of the feed being used as one of the key parameters determining the sustainability of production of a particular species particularly the fish-meal and fish oil content and how it relates to overall feed conversion ratios

Further formulation trends are moving towards low fishmeal and oil content in the grower feeds and finishing feeds designed to restore flesh quality parameters such as omega 3 fats and fillet quality that are con-sidered essential for the promotion of the product and its benefits for human health

This strategy addresses to some extent the limited resources of fishmeal and fish oil

Expectations of sustainabilityThe only way to achieve the expecta-

tions of sustainability and replace or supple-ment fishmeal and fish oil in aquafeeds is to exploit alternative protein and lipid sources

The obvious and traditionally used alter-natives are animal and plant protein sources

However in Europe and now increas-ingly in North America consumers pre-fer aquaculture products to have been

fed without the use of animal by-products due in part to recent health scares in the terrestrial animal sec-tor such as bovine spongiform encepha-lopathy (BSE)

The search is on therefore for not only the simple replacement of protein but the provi-sion of functional nutri-tion addressing both the sustainability and flesh quality requirements of the industry while

meeting the nutritional requirement of the animal being cultured

Alternative vegetable protein sources

Over the last two decades aquaculture research has focused on plant protein sources such as grains pulses and oilseeds as they are generally widely available

However the use of soybean meal and other plant based protein sources such as legume seeds oilseed cakes leaf meals leaf protein concentrates and root tuber meals have been limited due to the presence of a wide variety of anti-nutritional substances in these products and their interaction with the physiology of the animal resulting in depressed growth and feed efficiency mortality and abnormal intestinal morphol-ogy and damage

These substances act in different ways by affecting protein utilisation and digestion (eg protease inhibitors tannins lectins) by affecting mineral utilisation (eg phytate gossypol pigments glucosinolates) antivita-mins and other miscellaneous substances (eg phytoestrogens)

The increasing use of plant protein sources is also raising new concerns about possible contamination by mould derived mycotoxins

This risk is enhanced by the global nature and trading of plant protein sourc-es and the as yet little investigated impact of different mycotoxins and their interac-tions in aquaculture species In 2008 the Rapid Alert System for Food and Feed in

Protein sources Alternative protein sources should also show functionality if they are to play a role in modern aquafeedsby Keith Filer PhD research Manager Asia-Pacific biosciences Centre Alltech inc

Figure 1 A comparison of typical essential amino acid (EAA) requirements for fish and the EAA profile of AquateTM

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

juns

wor

th |

Dre

amst

ime

com

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

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16 | InternatIonal AquAFeed | March-april 2010

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Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

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20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

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of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

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Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

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PROFILE Technology

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Technical SpecificationInternational Aquafeed magazine feature article reprints

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bull For use as Point of sale materials exhibition handouts etc

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Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

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Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

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________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

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E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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(UKpound15 per copy)

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Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

You want to know more about our event Then please visit wwwsummit2010org

Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

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MY

CY

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idl10 - Grain Feedai 1222010 45438 PM

40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

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M

Y

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idl10 - Grain Feedai 1222010 45438 PM

Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

bull Crevetecbull Danisco Animal Nutritionbull Exquimbull Ge-Pro Aquabull Inve Nutri-Ad

Elevator buckets

Equipment for salebull A Foeth BVbull Condex (UK) Ltd

bull Filter Screen Supply Ltd

Extrudersbull Almex

bull Anderson Internationalbull Baker Perkinsbull Brabender

bull Caliber Controlbull Conforma Clad Incbull Coperionbull ESE Intecbull Fudex Group Spabull Insta Pro International

bull Setrembull Suehiro EPMbull TGC Extrusionbull Wenger

Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 9: March | April 10 - International Aquafeed

stocks including reproduc-tion breeding and genetic improvements

bull Species diversification - finfish including the culture of flatfish tuna and other lsquonewrsquo finfish species for future commercial production

bull Species diversification ndash other species including the culture of octopus cuttlefish abalone crabs seaweeds and other lsquonewrsquo species for future commercial production

bull Production in different envi-ronments and produc-tion technologies including sessions on

bull Land-based production - new production technol-ogies including large-scale RAS production in ar id regions and other land based activities

bull Offshore aquaculture of fish and shellfish

bull Aquaculture on Islands ndash specificities of production in outlying areas in tropical and temperate regions including European outer-most regions

Tools for the development of the sector including sessions on

bull The eco-system approach to aquaculture produc-tion including semi inten-siveextensive coastallagoon aquaculture nutrient man-agement and IMTA invasive species predator control nature management and pat-rimonial conservation

bull Space for aquaculture Carrying capacity and Spatial Planning

bull Aquaculture governance and interactions with fish-eries including policy soci-oeconomic and economic research and interactions commonalities and interde-pendence with fisheries

bull Health quality and well-being including sessions on

bull Human health consumer science and quality including

aquaculture percept ion studies biotoxins quality assessment and detection certification

bull Health management of finfish and shellfish Sponsored by Inter vet Schering-Plough Animal Health

bull Novel feeds ingredients and additives Sponsored by Biomin

bull Aquaculture welfare and ethics

The conference will also include a special Student Workshop and naturally a student reception

Science meets industryAquaculture Europe 2010 will

also feature a special interna-tional trade exhibition organised by the European Aquaculture Society Por tuguese Spanish and International companies will present the latest products and ser vices for aquacul-ture A special farmersrsquo day programme will have an emphasis on aquaculture in Por tugal and in Spain and will focus on topics where the latest research findings can have an impact on the sector

It is hoped that the main Por tuguese and Spanish industr y associations for finfish and shel l fish wil l take advantage of the farmersrsquo day to bring their members to Por to and to use the event for their own meetings

Good local and industry support

AE2010 i s f inanc ia l ly suppor ted by the Area Metropolitana do Porto

The gold sponsor of AE2010 is Sorgal SA

E A S i s p r o u d t o announce the AE2010 Gold Sponsorship of Portuguese a q u a f e e d c o m p a n y Aquasoja Aquasoja is the fish feed brand of Sorgal SA Sorgal SA is par t of a

large Por tuguese agro-indus-trial group Sorgal has produced quality feeds for animal farming for more than 50 years and has always conducted its activities focussing on cost-effectiveness animal welfare and feed safety as top priorities

As par t o f i t s Premier Sponsorship of the European Aquaculture Society Inter vet Schering Plough Animal Health will have a strong presence and will sponsor the health manage-ment session

Feed ingredients and addi-t ives specia l i s t B iomin wi l l sponsor the session on Novel feeds ingredients and addi-tives

Those interested in booking exhibition space andor sponsor-ship opportunities should contact Mario Stael at mariostaelscarletbe

EU to showcase FP6 research

The EU Forum ndash a regular feature of Aquaculture Europe events ndash is currently being planned and will look to showcase the results of two of the biggest integrated research projects carried out by the Commission in aquaculture as well as presenting the view of national research funders The EU Forum will also look forward to the final stages of FP7

Just pr ior to AE2010 the Larvanet network will meet in Porto The latest findings of their research will be presented in a special session within the confer-ence A series of short workshops organised by other EU-funded projects and other relevant ini-tiatives will also take place in the exhibition area and specifically targeted towards exhibitors and trade show visitors

6 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 7

Aqua News

Kiotechagil the interna-tional supplier of natural high performance feed

additives to enhance growth health and sustainability in agri-culture and aquaculture will have a strong presence at VIV in The Netherlands in April 2010

The company will be high-lighting their new acidifier pHorce which compliments their range of acid products pHorce will provide a concen-trated acid on a micropearl silica carrier and can be used for decontamination of raw materials as an alternative to

c o r r o s i v e liquids and in finished feed at one kgtonne

In addition Kiotechagil will be showcasing a new broad spectrum anti-mycotoxin product which will combine the benefits of their highly effective alumino-silicate Sorbatox with a primary growth yeast cell wall compo-nent high in both beta glucans and mannan oligosaccharides

Mo re in fo rMatio n

Kiotechagil VIV Stand No C066 in Hall 9Tel +44 1189 813333Email infokiotechagilcom

Kiotechagil to show pHorceful presence at VIV

Animal nutrition company Norel launched a new website wwwnorel

es that will provide up-to-date information about its products and the company activities

Founded in 1980 by Mr Enrique Pablos Perez Norel SA has been steadily growing and developing into a well known brand The original meaning of Norel was the Spanish phrase lsquoNO REconocemos Limitesrsquo l i teral ly meaning lsquoWe donrsquot acknowledge limitsrsquo thus dem-onstrating Norelrsquos philosophy The Skyrsquos The Limit or in other words the possibi l i t ies are endless

Norel has become associated with dynamism and innovation (more than five percent of its turnover is invested in Research and Development) and also with quality and performance thanks to our ISO GMP and FAMI-QS certifications

An important feature of Norel is its focus since the beginning on animal nutrition

Originally Norel was a supplier of raw mater ials for animal nutrition milk replacers whey products and protein extracts In 1982 it star ted the production

of spray fat concentrates as well as organic trace minerals (methionates)

Expansion and develop-ment followed and in 1988 Norel opened i ts b io-technology plant in Leon (Spain)

ldquo We t h e n b e c a m e the fi r st b iotechnology company in Spain spe-cialised in animal nutrition In 1990 we star ted the production of by-pass fats for ruminants Today with our flagship Magnapac we are one of the major pro-ducers worldwide Our devel-opment continued with new factories in Egypt and Mexico and specialized units in Spainrdquo says the company in an inter-view with the press

ldquoAn impor tant date in our history is certainly 2001 when Norel SA merged its activities with Nature SA a company spe-cialized in the production of flavours and sweeteners acidi-fiers antioxidants and mould inhibitors

ldquoWe then became known in the market as lsquoNorel amp Naturersquo at that time it was impor tant for us to keep the name Nature

because our act iv it ies and products were different but at the same time very complemen-tary Today we are pleased to introduce our new logo together with the launch of our brand new website wwwnoreles

The new logo of Norel takes into account the histor y by combin ing the two Nrsquos of Norel and Nature Together they resemble the famous Yin Yang symbol and remind us that the two entities now make one The new logo also represents the infinity symbol a reference to the or ig inal meaning of Norel continue to

grow and the possibilities are endless

I t s new webs i t e comes w i th an improved cor po-rate identity and many new features and updates Most impor tant ly i t has added a news section that wil l keep reader s updated on la test developments and activ it ies of Norel worldwide

Mo re in fo rMatio n

Norel SAJesus Aprendiz 19 1 A y BMadrid NC 28007 SpainTel +34 6698 09659Website wwwnorelnaturecom

NOREL announces new web site and new corporate image

8 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 9

Dishman Group o f Ahmedabad I nd i a a lead ing products

and ser vices provider to the pharmaceutical industr y has announced the appointment of Chris Oates to the position of chief operating officer

Mr Oates will repor t to Nick Green president of the Dishman Globa l Contr act Research and Manufactur ing Ser vices (CRAMS) business

M r O a t e s w i l l a s s u m e responsibil ity for Indian and Chinese operations within the company

He wi l l be managing the development operation and improvement of the systems in the two manufacturing sites in India (Bavla and Naroda) and

the new Shanghai facility which is due for completion mid-2010

In addition to the units in Naroda and China Mr Oates wil l be responsible for the nine operating units at Bavla including a large-scale high potency fac i l i ty which wi l l be inaugurated at the end of January The new state-of-the-ar t high potency facil ity will extend Carbogen Amcisrsquo man-ufactur ing capacity to multi-tonne scale and will be the first of its kind to be located in Asia

M r O a t e s rsquo e x p e r i e n c e combines both product ion and operational roles in the contract manufacturing arena He will play a pivotal role in

expanding the companyrsquos core CRAMS business

Mr Oates holds a bach-elorrsquos degree in applied chem-istr y from the University of Huddersfield in the UK His exper t ise is bui lt on more than 20 years working within the pharmaceutical industr y He most recently ser ved as managing director of Uquifa Mexico His previous expe-r ience includes six year s at Avecia Pharmaceuticals in the UK where he held the position of operations manager and as the position of senior pro-duction manager for Syngenta (Agrochemicals)

ldquo I am looking forward to taking up this new challenge and am very excited by what I

believe will be a very successful future for the companyrdquo said Mr Oates

ldquoDishman can provide the full continuum of ser vices from process RampD to large-scale commercial manufacturing in multiple locations around the globe The company is ideally p l aced to bu i ld upon i t s success to date and become the industr y leading CRAMS par tnerrdquo

Mo re in fo rMatio n

Dishman Europe LimitedSuite 4 De Walden Court 85 New Cavendish Street London W1W 6XDUnited KingdomTel +44 2073 230608 Fax +44 2073 230609Website wwwdishmangroupcom

Dishman appoints Chris Oates as chief operating officerNew role will oversee Dishman Indian and Chinese Facilities

Sponsorship of a keynote session at Aquaculture 2010 confirms Biominrsquos

firm commitment and keen focus on this dynamic growth sector

Biomin embraces the new year with a bold plunge in aqua-culture

Fo r the f i r s t t ime the company wil l be par tner ing the World Aquaculture Society (WAS) with the sponsorship of the Aquafeeds Session at Aquaculture 2010

To be held in San Diego from March 1-5 2010 Aquaculture 2010 is the largest event for the global aquaculture industry Hosted every year by the WAS the global aquaculture event is expected to draw some 4000 participants from over 80 coun-tr ies including high profi le

attendees who form the core of decision-makers and innova-tors in the aquatic business

Biomin is proud to be a session sponsor at the worldrsquos largest aquaculture event Held on March 4 2010 the session on lsquoAquafeedsrsquo will see exper t speakers from com-mercial practice and academia share their i n s i gh t s on ma jor issues in aquafeeds formulation and alter-nat ives to fishmeal and protein replace-ment strategies for a variety of aquatic species

Years of dedicated applied research and investments have strengthened Biominrsquos drive and reputation in providing effective natural-based feed solutions for the aquaculture and aquafeed

business throughout the world A core focus on fundamental applied research forms the basis of Biominrsquos portfolio of science-based aquaculture product solu-tions Biominreg AquaSpecials which includes nutraceuticals probiotics natural growth pro-

moters mycotoxin deactiva-tors and functional feed addi-tives

Dr Pedro Encarnaccedilatildeo Biominrsquos aquacul ture spec ia l i s t says ldquoBiomin is a research-focused and -driven company

ldquoWe have translated our long-

standing competence in animal nutrition for terrestrial species into aquaculture and aquafeed research Our Aquatic Centre for Applied Animals (AQUACAN) in Thailand actively conducts feeding trials for many aquatic species from which new findings

have been translated into innovative and practical solutions in aquafeedsrdquo

Biomin will also par-ticipate as a sponsor at the Australas ian Aquacu l ture 2010

another WAS event to be held in Hobar t Australia in May 2010 and at the Aquaculture Europe that will take place in Por to Por tugal in October 2010 both of which are key events for aquaculture in the coming year

A bold step for BIOMIN aquacultureBiomin proud to be session sponsor at the worldrsquos largest aquaculture event

8 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 9

Aqua News

Tecnoloacutegica de Alimentos SA (TASA) of Peru is the first fishmeal and fish

oil producer to achieve cer ti-fication under the new IFFO Global Standard for Responsible Supply

The cer tification has been awarded to the Callao Nor te plant of TASA the worldrsquos largest fishmeal producer Earlier this week Jorge Mora Operations Director of IFFO presented the

IFFO-Assured certificate to Mario Brescia CEO of TASA at its head-quarters in Lima Peru

IFFO has been developing its RS Standard since early 2008 with the help of a Technical Advisory Committee comprising repre-sentatives from the whole aqua-culture value chain including fish farmers feed producers and retailers and also NGOs

This business-to-business inde-pendently-audited programme will enable IFFO members to demonstrate to their customers that they are offer ing pure marine ingredients manufactured from responsibly sourced and traceable raw materials Whole fish used must come from fish-eries managed under the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

ldquoSince October 2009 when we launched the programme we have had applications from 15 manufacturing companies in four major producing countries sourcing from 10 fisheriesrdquo says IFFO director general Jonathan Shepherd

ldquoWe anticipate that there will be substantial supplies of IFFO-Assured fishmeal and fish oil on the market within months and that up to one third of global

p r o d u c t i o n could be certi-fied by the end of 2010rdquo

ldquoWith th i s p r o g r a m m e IFFO has put in place the first link in a fully certified supply chain for aqua-culture and for land animals such as pigs and poul tr y

It will help the industry demon-strate its commitment to respon-sible manufacture of fishmeal and fish oil as well as to the FAO Code of Conductrdquo said Dr Shepherd

Applicant factories which can demonstrate that they are ldquooper-ating a well managed system that ensures their marine raw mate-rials are responsibly sourced and processed to the IFFO Standardrdquo will be awarded certification

They will be able to offer IFFO-Assured fishmeal and fish oil and have the right to display the RS logo An up-to-date list of cur-rently certified fishmeal plants will be posted on the IFFO web site alongside the full Standard

Characteristics of the IFFO Global Standard for Responsible Supply (RS)

bull Standards set by a multi-stake-holder Technical Advisory

Committee including pro-ducers traders fish farmers feed manufacturers proc-essors retailers standard setting organisations and environmental NGOs The Assessment Management and Certification Process are contracted to Global Trust

bull A programme independ-ently administered by an ISO 651996 accredited certifica-tion body which is a member of the International Accreditation Forum (IFA)

bull I t d e m o n s t r a t e s responsible sourcing o f t r aceable r aw material and respon-sible production to ensure feed safety

bull A business-to-busi-ness tool designed to enable fishmeal and fish oil producers to demonstrate the integr i ty o f the i r products and their processes to their cus-tomers in the value-chain rather than a consumer eco-label

bull Whole fish used must come from fisheries that have been independently assessed and meet the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

bull Exclusion of illegal unre-por ted and unregulated (IUU) fish

bull The factor y must have attained Cer tification to the Internat ional Feed Ingredients Standard (IFIS) of the International Feed Safety Alliance (IFSA) or equiva-lent as proof of responsible manufacturing and the appli-cant must be in possession

of all relevant permits and licenses for the production and sale of fishmeal and fish oil products

bull This programme is com-patible with ISO 651996 (General requirements for bodies operating product cer tification systems) and with EN45011

bull Recogn i ses other cer-t i f i c a t ion p rog r ammes w h i c h h av e d e m o n -

strated equivalency and which are accepted within the industry - eg Marine Stewardship Council (MSC) for the fishery certification element only

The IFFO programme will continue to be developed in 2010 addressing further aspects of responsible supply

Mo re in fo rMatio n

Andrew JacksonTechnical Director IFFO 2 College YardLower Dagnall Street St Albans Hertfordshire AL3 4PAUnited KingdomTel +44 7808 783488 Email ajacksoniffonetWebiste wwwiffonet

First certification awarded under the IFFO Global Standard for Responsible Supply

ldquoThe first link in a fully certified aquaculture supply chain is in placerdquo

ldquoSupplies of ldquoIFFO-Assuredrdquo fishmeal and fish oil will shortly be on the marketrdquo

Jorge Mora (right) Operations Director of IFFO presenting the IFFO-Assured certificate to Mario Brescia (left) CEO of TASA at its headquarters in Lima Peru

10 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 11

Me r c a t o r M e d i a o r g a n i s e r o f t h e Offshore Mariculture

Conference 2010 is pleased to announce that the leading i ndus t r y a s soc i a t ions w i l l be suppor t i n g t he 2010 Conference

The European Aquaculture Techno logy amp I nnova t ion Platform (EATiP) the Federation o f Eu ropean Aquacu l tu re Producers (FEAP) the Global

Aquaculture All iance (GAA) and HGK Grupacija akvakul-ture the aquaculture affi l ia-tion of the Croatian Chamber of Economy (and member of FEAP) have all pledged their suppor t to the Conference that wi l l be held from the

June 16-18 2010 at the Hilton Imper ial Hotel Dubrovnik Croatia

Both EATiP (European Aquaculture Technology amp Innovation Platform) and FEAP (Federation of European Aquaculture Producers) are pleased to suppor t the Offshore Mariculture Conference s ince events such as these give fish farmers t h e o p p o r t u -n i t y t o l e a r n from both indus-t r i a l and RTD

exper ts about relevant technological advances to rece ive in formed updates on key research and policy issues and

to be able to network with col leagues from across the sector says Cour tney Hough general secretary of FEAP and EATiP

As wel l as promoting the event to their members and therefore rais ing awareness of the event and its presti-gious speakers it also means that members of any of the above assoc ia t ions wi l l be en t i t l ed to a 10 pe rcen t d i s count o f f the de lega te rate European Aquaculture Society members will also be ent i t led to the discount as the EAS have continued their suppor t f rom the prev ious even t he ld i n A l i c an te i n 2008

The technical conference will be held on the June 16 and 17 June with a visit to a fish farm on the June 18 2010 The Conference Networking Dinner will take place on the evening of Wednesday June 16 2010 at the fabulous Revelin For tress which was bui lt in 1462 to provide protection to the Eastern City Gate Place Over look ing the Old Ci ty Harbour the Revelins terrace offers what are probably the best views of the Old City and its famous Harbour

Mo re in fo rMatio n

Offshore MaricultureTel +44 1622 820622Website wwwoffshoremariculturecom

Industry support strengthens for 2010 OSM Conference

The full programme will be released shortly sessions will include

bull Policy legal framework and key research updates

bull Practical offshore farming operation

bull Spatial planning and moni-toring

bull Spotlight on Croatiabull Ecological monitoring and

future technologiesbull European and International

case studies

iN THE NEWSYour news could feature in our pages

Do you have an news item that you would like to see in our pages Send your press releases to the Production Manager - Nicky BarnesEmail nickybaquafeedcouk

10 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 11

Aqua News

The aquaculture industry today faces a number of challenges that threaten to restrict its development

and profitability

To live in harmony with consumer expec-tations of the highest possible standards of product quality and to do so in a sustainable and environmentally responsible manner necessitates an innovative coordinated and holistic approach within the aquaculture industry

The requirement for sustainability has focused on the composition of the feed being used as one of the key parameters determining the sustainability of production of a particular species particularly the fish-meal and fish oil content and how it relates to overall feed conversion ratios

Further formulation trends are moving towards low fishmeal and oil content in the grower feeds and finishing feeds designed to restore flesh quality parameters such as omega 3 fats and fillet quality that are con-sidered essential for the promotion of the product and its benefits for human health

This strategy addresses to some extent the limited resources of fishmeal and fish oil

Expectations of sustainabilityThe only way to achieve the expecta-

tions of sustainability and replace or supple-ment fishmeal and fish oil in aquafeeds is to exploit alternative protein and lipid sources

The obvious and traditionally used alter-natives are animal and plant protein sources

However in Europe and now increas-ingly in North America consumers pre-fer aquaculture products to have been

fed without the use of animal by-products due in part to recent health scares in the terrestrial animal sec-tor such as bovine spongiform encepha-lopathy (BSE)

The search is on therefore for not only the simple replacement of protein but the provi-sion of functional nutri-tion addressing both the sustainability and flesh quality requirements of the industry while

meeting the nutritional requirement of the animal being cultured

Alternative vegetable protein sources

Over the last two decades aquaculture research has focused on plant protein sources such as grains pulses and oilseeds as they are generally widely available

However the use of soybean meal and other plant based protein sources such as legume seeds oilseed cakes leaf meals leaf protein concentrates and root tuber meals have been limited due to the presence of a wide variety of anti-nutritional substances in these products and their interaction with the physiology of the animal resulting in depressed growth and feed efficiency mortality and abnormal intestinal morphol-ogy and damage

These substances act in different ways by affecting protein utilisation and digestion (eg protease inhibitors tannins lectins) by affecting mineral utilisation (eg phytate gossypol pigments glucosinolates) antivita-mins and other miscellaneous substances (eg phytoestrogens)

The increasing use of plant protein sources is also raising new concerns about possible contamination by mould derived mycotoxins

This risk is enhanced by the global nature and trading of plant protein sourc-es and the as yet little investigated impact of different mycotoxins and their interac-tions in aquaculture species In 2008 the Rapid Alert System for Food and Feed in

Protein sources Alternative protein sources should also show functionality if they are to play a role in modern aquafeedsby Keith Filer PhD research Manager Asia-Pacific biosciences Centre Alltech inc

Figure 1 A comparison of typical essential amino acid (EAA) requirements for fish and the EAA profile of AquateTM

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

juns

wor

th |

Dre

amst

ime

com

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

040

120

08

180

120

08

010

220

08

150

220

08

290

220

08

140

320

08

280

320

08

110

420

08

250

420

08

090

520

08

230

520

08

16 | InternatIonal AquAFeed | March-april 2010

Alltechreg has developed a range of products to address dietary requirements at different

stages of life in aquaculture species These sustainable product solutions are proven to

support profitability in aquaculture operations as well as performance in fish and shrimp

in the Family

Give your fish and shrimp the Alltech Aqua Advantage Contact your Alltech representative today for more information

trade

aquasolutionsalltechcomwwwalltechcom

Ideally suited for fish and shrimp in any stage of life to maintain performance and to support independence from traditional aquaculture diet ingredients

Specifically designed for the juvenile stages of life in fish and shrimp to support good health uniform growth and survival

A natural affordable product created for salmon in all life stages to maintain good health and defense mechanisms against adverse aquatic elements present in the surrounding environment

trade

trade

Aquate Ad for IAF due Feb 19 2010V2indd 1 2192010 115722 AM

Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

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Delivery charged on a case by case basis

For more details please visit wwwaquafeedcoukreprints

1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

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21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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________________________________________________________

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20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

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19th - 20th May 10 AQUACULTURE UK 2010

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23rd - 26th May 10 Australasian Aquaculture 2010

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T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

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16th - 18th June 10Offshore Mariculture Conference 2010

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5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

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T +34 93 2212955 | F +34 93 4592059

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International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

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Date 10 February 2010 212628 GMT

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International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

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DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 10: March | April 10 - International Aquafeed

Kiotechagil the interna-tional supplier of natural high performance feed

additives to enhance growth health and sustainability in agri-culture and aquaculture will have a strong presence at VIV in The Netherlands in April 2010

The company will be high-lighting their new acidifier pHorce which compliments their range of acid products pHorce will provide a concen-trated acid on a micropearl silica carrier and can be used for decontamination of raw materials as an alternative to

c o r r o s i v e liquids and in finished feed at one kgtonne

In addition Kiotechagil will be showcasing a new broad spectrum anti-mycotoxin product which will combine the benefits of their highly effective alumino-silicate Sorbatox with a primary growth yeast cell wall compo-nent high in both beta glucans and mannan oligosaccharides

Mo re in fo rMatio n

Kiotechagil VIV Stand No C066 in Hall 9Tel +44 1189 813333Email infokiotechagilcom

Kiotechagil to show pHorceful presence at VIV

Animal nutrition company Norel launched a new website wwwnorel

es that will provide up-to-date information about its products and the company activities

Founded in 1980 by Mr Enrique Pablos Perez Norel SA has been steadily growing and developing into a well known brand The original meaning of Norel was the Spanish phrase lsquoNO REconocemos Limitesrsquo l i teral ly meaning lsquoWe donrsquot acknowledge limitsrsquo thus dem-onstrating Norelrsquos philosophy The Skyrsquos The Limit or in other words the possibi l i t ies are endless

Norel has become associated with dynamism and innovation (more than five percent of its turnover is invested in Research and Development) and also with quality and performance thanks to our ISO GMP and FAMI-QS certifications

An important feature of Norel is its focus since the beginning on animal nutrition

Originally Norel was a supplier of raw mater ials for animal nutrition milk replacers whey products and protein extracts In 1982 it star ted the production

of spray fat concentrates as well as organic trace minerals (methionates)

Expansion and develop-ment followed and in 1988 Norel opened i ts b io-technology plant in Leon (Spain)

ldquo We t h e n b e c a m e the fi r st b iotechnology company in Spain spe-cialised in animal nutrition In 1990 we star ted the production of by-pass fats for ruminants Today with our flagship Magnapac we are one of the major pro-ducers worldwide Our devel-opment continued with new factories in Egypt and Mexico and specialized units in Spainrdquo says the company in an inter-view with the press

ldquoAn impor tant date in our history is certainly 2001 when Norel SA merged its activities with Nature SA a company spe-cialized in the production of flavours and sweeteners acidi-fiers antioxidants and mould inhibitors

ldquoWe then became known in the market as lsquoNorel amp Naturersquo at that time it was impor tant for us to keep the name Nature

because our act iv it ies and products were different but at the same time very complemen-tary Today we are pleased to introduce our new logo together with the launch of our brand new website wwwnoreles

The new logo of Norel takes into account the histor y by combin ing the two Nrsquos of Norel and Nature Together they resemble the famous Yin Yang symbol and remind us that the two entities now make one The new logo also represents the infinity symbol a reference to the or ig inal meaning of Norel continue to

grow and the possibilities are endless

I t s new webs i t e comes w i th an improved cor po-rate identity and many new features and updates Most impor tant ly i t has added a news section that wil l keep reader s updated on la test developments and activ it ies of Norel worldwide

Mo re in fo rMatio n

Norel SAJesus Aprendiz 19 1 A y BMadrid NC 28007 SpainTel +34 6698 09659Website wwwnorelnaturecom

NOREL announces new web site and new corporate image

8 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 9

Dishman Group o f Ahmedabad I nd i a a lead ing products

and ser vices provider to the pharmaceutical industr y has announced the appointment of Chris Oates to the position of chief operating officer

Mr Oates will repor t to Nick Green president of the Dishman Globa l Contr act Research and Manufactur ing Ser vices (CRAMS) business

M r O a t e s w i l l a s s u m e responsibil ity for Indian and Chinese operations within the company

He wi l l be managing the development operation and improvement of the systems in the two manufacturing sites in India (Bavla and Naroda) and

the new Shanghai facility which is due for completion mid-2010

In addition to the units in Naroda and China Mr Oates wil l be responsible for the nine operating units at Bavla including a large-scale high potency fac i l i ty which wi l l be inaugurated at the end of January The new state-of-the-ar t high potency facil ity will extend Carbogen Amcisrsquo man-ufactur ing capacity to multi-tonne scale and will be the first of its kind to be located in Asia

M r O a t e s rsquo e x p e r i e n c e combines both product ion and operational roles in the contract manufacturing arena He will play a pivotal role in

expanding the companyrsquos core CRAMS business

Mr Oates holds a bach-elorrsquos degree in applied chem-istr y from the University of Huddersfield in the UK His exper t ise is bui lt on more than 20 years working within the pharmaceutical industr y He most recently ser ved as managing director of Uquifa Mexico His previous expe-r ience includes six year s at Avecia Pharmaceuticals in the UK where he held the position of operations manager and as the position of senior pro-duction manager for Syngenta (Agrochemicals)

ldquo I am looking forward to taking up this new challenge and am very excited by what I

believe will be a very successful future for the companyrdquo said Mr Oates

ldquoDishman can provide the full continuum of ser vices from process RampD to large-scale commercial manufacturing in multiple locations around the globe The company is ideally p l aced to bu i ld upon i t s success to date and become the industr y leading CRAMS par tnerrdquo

Mo re in fo rMatio n

Dishman Europe LimitedSuite 4 De Walden Court 85 New Cavendish Street London W1W 6XDUnited KingdomTel +44 2073 230608 Fax +44 2073 230609Website wwwdishmangroupcom

Dishman appoints Chris Oates as chief operating officerNew role will oversee Dishman Indian and Chinese Facilities

Sponsorship of a keynote session at Aquaculture 2010 confirms Biominrsquos

firm commitment and keen focus on this dynamic growth sector

Biomin embraces the new year with a bold plunge in aqua-culture

Fo r the f i r s t t ime the company wil l be par tner ing the World Aquaculture Society (WAS) with the sponsorship of the Aquafeeds Session at Aquaculture 2010

To be held in San Diego from March 1-5 2010 Aquaculture 2010 is the largest event for the global aquaculture industry Hosted every year by the WAS the global aquaculture event is expected to draw some 4000 participants from over 80 coun-tr ies including high profi le

attendees who form the core of decision-makers and innova-tors in the aquatic business

Biomin is proud to be a session sponsor at the worldrsquos largest aquaculture event Held on March 4 2010 the session on lsquoAquafeedsrsquo will see exper t speakers from com-mercial practice and academia share their i n s i gh t s on ma jor issues in aquafeeds formulation and alter-nat ives to fishmeal and protein replace-ment strategies for a variety of aquatic species

Years of dedicated applied research and investments have strengthened Biominrsquos drive and reputation in providing effective natural-based feed solutions for the aquaculture and aquafeed

business throughout the world A core focus on fundamental applied research forms the basis of Biominrsquos portfolio of science-based aquaculture product solu-tions Biominreg AquaSpecials which includes nutraceuticals probiotics natural growth pro-

moters mycotoxin deactiva-tors and functional feed addi-tives

Dr Pedro Encarnaccedilatildeo Biominrsquos aquacul ture spec ia l i s t says ldquoBiomin is a research-focused and -driven company

ldquoWe have translated our long-

standing competence in animal nutrition for terrestrial species into aquaculture and aquafeed research Our Aquatic Centre for Applied Animals (AQUACAN) in Thailand actively conducts feeding trials for many aquatic species from which new findings

have been translated into innovative and practical solutions in aquafeedsrdquo

Biomin will also par-ticipate as a sponsor at the Australas ian Aquacu l ture 2010

another WAS event to be held in Hobar t Australia in May 2010 and at the Aquaculture Europe that will take place in Por to Por tugal in October 2010 both of which are key events for aquaculture in the coming year

A bold step for BIOMIN aquacultureBiomin proud to be session sponsor at the worldrsquos largest aquaculture event

8 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 9

Aqua News

Tecnoloacutegica de Alimentos SA (TASA) of Peru is the first fishmeal and fish

oil producer to achieve cer ti-fication under the new IFFO Global Standard for Responsible Supply

The cer tification has been awarded to the Callao Nor te plant of TASA the worldrsquos largest fishmeal producer Earlier this week Jorge Mora Operations Director of IFFO presented the

IFFO-Assured certificate to Mario Brescia CEO of TASA at its head-quarters in Lima Peru

IFFO has been developing its RS Standard since early 2008 with the help of a Technical Advisory Committee comprising repre-sentatives from the whole aqua-culture value chain including fish farmers feed producers and retailers and also NGOs

This business-to-business inde-pendently-audited programme will enable IFFO members to demonstrate to their customers that they are offer ing pure marine ingredients manufactured from responsibly sourced and traceable raw materials Whole fish used must come from fish-eries managed under the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

ldquoSince October 2009 when we launched the programme we have had applications from 15 manufacturing companies in four major producing countries sourcing from 10 fisheriesrdquo says IFFO director general Jonathan Shepherd

ldquoWe anticipate that there will be substantial supplies of IFFO-Assured fishmeal and fish oil on the market within months and that up to one third of global

p r o d u c t i o n could be certi-fied by the end of 2010rdquo

ldquoWith th i s p r o g r a m m e IFFO has put in place the first link in a fully certified supply chain for aqua-culture and for land animals such as pigs and poul tr y

It will help the industry demon-strate its commitment to respon-sible manufacture of fishmeal and fish oil as well as to the FAO Code of Conductrdquo said Dr Shepherd

Applicant factories which can demonstrate that they are ldquooper-ating a well managed system that ensures their marine raw mate-rials are responsibly sourced and processed to the IFFO Standardrdquo will be awarded certification

They will be able to offer IFFO-Assured fishmeal and fish oil and have the right to display the RS logo An up-to-date list of cur-rently certified fishmeal plants will be posted on the IFFO web site alongside the full Standard

Characteristics of the IFFO Global Standard for Responsible Supply (RS)

bull Standards set by a multi-stake-holder Technical Advisory

Committee including pro-ducers traders fish farmers feed manufacturers proc-essors retailers standard setting organisations and environmental NGOs The Assessment Management and Certification Process are contracted to Global Trust

bull A programme independ-ently administered by an ISO 651996 accredited certifica-tion body which is a member of the International Accreditation Forum (IFA)

bull I t d e m o n s t r a t e s responsible sourcing o f t r aceable r aw material and respon-sible production to ensure feed safety

bull A business-to-busi-ness tool designed to enable fishmeal and fish oil producers to demonstrate the integr i ty o f the i r products and their processes to their cus-tomers in the value-chain rather than a consumer eco-label

bull Whole fish used must come from fisheries that have been independently assessed and meet the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

bull Exclusion of illegal unre-por ted and unregulated (IUU) fish

bull The factor y must have attained Cer tification to the Internat ional Feed Ingredients Standard (IFIS) of the International Feed Safety Alliance (IFSA) or equiva-lent as proof of responsible manufacturing and the appli-cant must be in possession

of all relevant permits and licenses for the production and sale of fishmeal and fish oil products

bull This programme is com-patible with ISO 651996 (General requirements for bodies operating product cer tification systems) and with EN45011

bull Recogn i ses other cer-t i f i c a t ion p rog r ammes w h i c h h av e d e m o n -

strated equivalency and which are accepted within the industry - eg Marine Stewardship Council (MSC) for the fishery certification element only

The IFFO programme will continue to be developed in 2010 addressing further aspects of responsible supply

Mo re in fo rMatio n

Andrew JacksonTechnical Director IFFO 2 College YardLower Dagnall Street St Albans Hertfordshire AL3 4PAUnited KingdomTel +44 7808 783488 Email ajacksoniffonetWebiste wwwiffonet

First certification awarded under the IFFO Global Standard for Responsible Supply

ldquoThe first link in a fully certified aquaculture supply chain is in placerdquo

ldquoSupplies of ldquoIFFO-Assuredrdquo fishmeal and fish oil will shortly be on the marketrdquo

Jorge Mora (right) Operations Director of IFFO presenting the IFFO-Assured certificate to Mario Brescia (left) CEO of TASA at its headquarters in Lima Peru

10 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 11

Me r c a t o r M e d i a o r g a n i s e r o f t h e Offshore Mariculture

Conference 2010 is pleased to announce that the leading i ndus t r y a s soc i a t ions w i l l be suppor t i n g t he 2010 Conference

The European Aquaculture Techno logy amp I nnova t ion Platform (EATiP) the Federation o f Eu ropean Aquacu l tu re Producers (FEAP) the Global

Aquaculture All iance (GAA) and HGK Grupacija akvakul-ture the aquaculture affi l ia-tion of the Croatian Chamber of Economy (and member of FEAP) have all pledged their suppor t to the Conference that wi l l be held from the

June 16-18 2010 at the Hilton Imper ial Hotel Dubrovnik Croatia

Both EATiP (European Aquaculture Technology amp Innovation Platform) and FEAP (Federation of European Aquaculture Producers) are pleased to suppor t the Offshore Mariculture Conference s ince events such as these give fish farmers t h e o p p o r t u -n i t y t o l e a r n from both indus-t r i a l and RTD

exper ts about relevant technological advances to rece ive in formed updates on key research and policy issues and

to be able to network with col leagues from across the sector says Cour tney Hough general secretary of FEAP and EATiP

As wel l as promoting the event to their members and therefore rais ing awareness of the event and its presti-gious speakers it also means that members of any of the above assoc ia t ions wi l l be en t i t l ed to a 10 pe rcen t d i s count o f f the de lega te rate European Aquaculture Society members will also be ent i t led to the discount as the EAS have continued their suppor t f rom the prev ious even t he ld i n A l i c an te i n 2008

The technical conference will be held on the June 16 and 17 June with a visit to a fish farm on the June 18 2010 The Conference Networking Dinner will take place on the evening of Wednesday June 16 2010 at the fabulous Revelin For tress which was bui lt in 1462 to provide protection to the Eastern City Gate Place Over look ing the Old Ci ty Harbour the Revelins terrace offers what are probably the best views of the Old City and its famous Harbour

Mo re in fo rMatio n

Offshore MaricultureTel +44 1622 820622Website wwwoffshoremariculturecom

Industry support strengthens for 2010 OSM Conference

The full programme will be released shortly sessions will include

bull Policy legal framework and key research updates

bull Practical offshore farming operation

bull Spatial planning and moni-toring

bull Spotlight on Croatiabull Ecological monitoring and

future technologiesbull European and International

case studies

iN THE NEWSYour news could feature in our pages

Do you have an news item that you would like to see in our pages Send your press releases to the Production Manager - Nicky BarnesEmail nickybaquafeedcouk

10 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 11

Aqua News

The aquaculture industry today faces a number of challenges that threaten to restrict its development

and profitability

To live in harmony with consumer expec-tations of the highest possible standards of product quality and to do so in a sustainable and environmentally responsible manner necessitates an innovative coordinated and holistic approach within the aquaculture industry

The requirement for sustainability has focused on the composition of the feed being used as one of the key parameters determining the sustainability of production of a particular species particularly the fish-meal and fish oil content and how it relates to overall feed conversion ratios

Further formulation trends are moving towards low fishmeal and oil content in the grower feeds and finishing feeds designed to restore flesh quality parameters such as omega 3 fats and fillet quality that are con-sidered essential for the promotion of the product and its benefits for human health

This strategy addresses to some extent the limited resources of fishmeal and fish oil

Expectations of sustainabilityThe only way to achieve the expecta-

tions of sustainability and replace or supple-ment fishmeal and fish oil in aquafeeds is to exploit alternative protein and lipid sources

The obvious and traditionally used alter-natives are animal and plant protein sources

However in Europe and now increas-ingly in North America consumers pre-fer aquaculture products to have been

fed without the use of animal by-products due in part to recent health scares in the terrestrial animal sec-tor such as bovine spongiform encepha-lopathy (BSE)

The search is on therefore for not only the simple replacement of protein but the provi-sion of functional nutri-tion addressing both the sustainability and flesh quality requirements of the industry while

meeting the nutritional requirement of the animal being cultured

Alternative vegetable protein sources

Over the last two decades aquaculture research has focused on plant protein sources such as grains pulses and oilseeds as they are generally widely available

However the use of soybean meal and other plant based protein sources such as legume seeds oilseed cakes leaf meals leaf protein concentrates and root tuber meals have been limited due to the presence of a wide variety of anti-nutritional substances in these products and their interaction with the physiology of the animal resulting in depressed growth and feed efficiency mortality and abnormal intestinal morphol-ogy and damage

These substances act in different ways by affecting protein utilisation and digestion (eg protease inhibitors tannins lectins) by affecting mineral utilisation (eg phytate gossypol pigments glucosinolates) antivita-mins and other miscellaneous substances (eg phytoestrogens)

The increasing use of plant protein sources is also raising new concerns about possible contamination by mould derived mycotoxins

This risk is enhanced by the global nature and trading of plant protein sourc-es and the as yet little investigated impact of different mycotoxins and their interac-tions in aquaculture species In 2008 the Rapid Alert System for Food and Feed in

Protein sources Alternative protein sources should also show functionality if they are to play a role in modern aquafeedsby Keith Filer PhD research Manager Asia-Pacific biosciences Centre Alltech inc

Figure 1 A comparison of typical essential amino acid (EAA) requirements for fish and the EAA profile of AquateTM

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

juns

wor

th |

Dre

amst

ime

com

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

040

120

08

180

120

08

010

220

08

150

220

08

290

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08

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08

110

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16 | InternatIonal AquAFeed | March-april 2010

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Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

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20 | InternatIonal AquAFeed | March-april 2010

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of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

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Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

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PROFILE Technology

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Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

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Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

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Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

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________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

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E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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(UKpound15 per copy)

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per year plus a copy of theInternational Aquafeed Directory amp

Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

You want to know more about our event Then please visit wwwsummit2010org

Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

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EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

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Y

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

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Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

bull Crevetecbull Danisco Animal Nutritionbull Exquimbull Ge-Pro Aquabull Inve Nutri-Ad

Elevator buckets

Equipment for salebull A Foeth BVbull Condex (UK) Ltd

bull Filter Screen Supply Ltd

Extrudersbull Almex

bull Anderson Internationalbull Baker Perkinsbull Brabender

bull Caliber Controlbull Conforma Clad Incbull Coperionbull ESE Intecbull Fudex Group Spabull Insta Pro International

bull Setrembull Suehiro EPMbull TGC Extrusionbull Wenger

Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 11: March | April 10 - International Aquafeed

Dishman Group o f Ahmedabad I nd i a a lead ing products

and ser vices provider to the pharmaceutical industr y has announced the appointment of Chris Oates to the position of chief operating officer

Mr Oates will repor t to Nick Green president of the Dishman Globa l Contr act Research and Manufactur ing Ser vices (CRAMS) business

M r O a t e s w i l l a s s u m e responsibil ity for Indian and Chinese operations within the company

He wi l l be managing the development operation and improvement of the systems in the two manufacturing sites in India (Bavla and Naroda) and

the new Shanghai facility which is due for completion mid-2010

In addition to the units in Naroda and China Mr Oates wil l be responsible for the nine operating units at Bavla including a large-scale high potency fac i l i ty which wi l l be inaugurated at the end of January The new state-of-the-ar t high potency facil ity will extend Carbogen Amcisrsquo man-ufactur ing capacity to multi-tonne scale and will be the first of its kind to be located in Asia

M r O a t e s rsquo e x p e r i e n c e combines both product ion and operational roles in the contract manufacturing arena He will play a pivotal role in

expanding the companyrsquos core CRAMS business

Mr Oates holds a bach-elorrsquos degree in applied chem-istr y from the University of Huddersfield in the UK His exper t ise is bui lt on more than 20 years working within the pharmaceutical industr y He most recently ser ved as managing director of Uquifa Mexico His previous expe-r ience includes six year s at Avecia Pharmaceuticals in the UK where he held the position of operations manager and as the position of senior pro-duction manager for Syngenta (Agrochemicals)

ldquo I am looking forward to taking up this new challenge and am very excited by what I

believe will be a very successful future for the companyrdquo said Mr Oates

ldquoDishman can provide the full continuum of ser vices from process RampD to large-scale commercial manufacturing in multiple locations around the globe The company is ideally p l aced to bu i ld upon i t s success to date and become the industr y leading CRAMS par tnerrdquo

Mo re in fo rMatio n

Dishman Europe LimitedSuite 4 De Walden Court 85 New Cavendish Street London W1W 6XDUnited KingdomTel +44 2073 230608 Fax +44 2073 230609Website wwwdishmangroupcom

Dishman appoints Chris Oates as chief operating officerNew role will oversee Dishman Indian and Chinese Facilities

Sponsorship of a keynote session at Aquaculture 2010 confirms Biominrsquos

firm commitment and keen focus on this dynamic growth sector

Biomin embraces the new year with a bold plunge in aqua-culture

Fo r the f i r s t t ime the company wil l be par tner ing the World Aquaculture Society (WAS) with the sponsorship of the Aquafeeds Session at Aquaculture 2010

To be held in San Diego from March 1-5 2010 Aquaculture 2010 is the largest event for the global aquaculture industry Hosted every year by the WAS the global aquaculture event is expected to draw some 4000 participants from over 80 coun-tr ies including high profi le

attendees who form the core of decision-makers and innova-tors in the aquatic business

Biomin is proud to be a session sponsor at the worldrsquos largest aquaculture event Held on March 4 2010 the session on lsquoAquafeedsrsquo will see exper t speakers from com-mercial practice and academia share their i n s i gh t s on ma jor issues in aquafeeds formulation and alter-nat ives to fishmeal and protein replace-ment strategies for a variety of aquatic species

Years of dedicated applied research and investments have strengthened Biominrsquos drive and reputation in providing effective natural-based feed solutions for the aquaculture and aquafeed

business throughout the world A core focus on fundamental applied research forms the basis of Biominrsquos portfolio of science-based aquaculture product solu-tions Biominreg AquaSpecials which includes nutraceuticals probiotics natural growth pro-

moters mycotoxin deactiva-tors and functional feed addi-tives

Dr Pedro Encarnaccedilatildeo Biominrsquos aquacul ture spec ia l i s t says ldquoBiomin is a research-focused and -driven company

ldquoWe have translated our long-

standing competence in animal nutrition for terrestrial species into aquaculture and aquafeed research Our Aquatic Centre for Applied Animals (AQUACAN) in Thailand actively conducts feeding trials for many aquatic species from which new findings

have been translated into innovative and practical solutions in aquafeedsrdquo

Biomin will also par-ticipate as a sponsor at the Australas ian Aquacu l ture 2010

another WAS event to be held in Hobar t Australia in May 2010 and at the Aquaculture Europe that will take place in Por to Por tugal in October 2010 both of which are key events for aquaculture in the coming year

A bold step for BIOMIN aquacultureBiomin proud to be session sponsor at the worldrsquos largest aquaculture event

8 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 9

Aqua News

Tecnoloacutegica de Alimentos SA (TASA) of Peru is the first fishmeal and fish

oil producer to achieve cer ti-fication under the new IFFO Global Standard for Responsible Supply

The cer tification has been awarded to the Callao Nor te plant of TASA the worldrsquos largest fishmeal producer Earlier this week Jorge Mora Operations Director of IFFO presented the

IFFO-Assured certificate to Mario Brescia CEO of TASA at its head-quarters in Lima Peru

IFFO has been developing its RS Standard since early 2008 with the help of a Technical Advisory Committee comprising repre-sentatives from the whole aqua-culture value chain including fish farmers feed producers and retailers and also NGOs

This business-to-business inde-pendently-audited programme will enable IFFO members to demonstrate to their customers that they are offer ing pure marine ingredients manufactured from responsibly sourced and traceable raw materials Whole fish used must come from fish-eries managed under the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

ldquoSince October 2009 when we launched the programme we have had applications from 15 manufacturing companies in four major producing countries sourcing from 10 fisheriesrdquo says IFFO director general Jonathan Shepherd

ldquoWe anticipate that there will be substantial supplies of IFFO-Assured fishmeal and fish oil on the market within months and that up to one third of global

p r o d u c t i o n could be certi-fied by the end of 2010rdquo

ldquoWith th i s p r o g r a m m e IFFO has put in place the first link in a fully certified supply chain for aqua-culture and for land animals such as pigs and poul tr y

It will help the industry demon-strate its commitment to respon-sible manufacture of fishmeal and fish oil as well as to the FAO Code of Conductrdquo said Dr Shepherd

Applicant factories which can demonstrate that they are ldquooper-ating a well managed system that ensures their marine raw mate-rials are responsibly sourced and processed to the IFFO Standardrdquo will be awarded certification

They will be able to offer IFFO-Assured fishmeal and fish oil and have the right to display the RS logo An up-to-date list of cur-rently certified fishmeal plants will be posted on the IFFO web site alongside the full Standard

Characteristics of the IFFO Global Standard for Responsible Supply (RS)

bull Standards set by a multi-stake-holder Technical Advisory

Committee including pro-ducers traders fish farmers feed manufacturers proc-essors retailers standard setting organisations and environmental NGOs The Assessment Management and Certification Process are contracted to Global Trust

bull A programme independ-ently administered by an ISO 651996 accredited certifica-tion body which is a member of the International Accreditation Forum (IFA)

bull I t d e m o n s t r a t e s responsible sourcing o f t r aceable r aw material and respon-sible production to ensure feed safety

bull A business-to-busi-ness tool designed to enable fishmeal and fish oil producers to demonstrate the integr i ty o f the i r products and their processes to their cus-tomers in the value-chain rather than a consumer eco-label

bull Whole fish used must come from fisheries that have been independently assessed and meet the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

bull Exclusion of illegal unre-por ted and unregulated (IUU) fish

bull The factor y must have attained Cer tification to the Internat ional Feed Ingredients Standard (IFIS) of the International Feed Safety Alliance (IFSA) or equiva-lent as proof of responsible manufacturing and the appli-cant must be in possession

of all relevant permits and licenses for the production and sale of fishmeal and fish oil products

bull This programme is com-patible with ISO 651996 (General requirements for bodies operating product cer tification systems) and with EN45011

bull Recogn i ses other cer-t i f i c a t ion p rog r ammes w h i c h h av e d e m o n -

strated equivalency and which are accepted within the industry - eg Marine Stewardship Council (MSC) for the fishery certification element only

The IFFO programme will continue to be developed in 2010 addressing further aspects of responsible supply

Mo re in fo rMatio n

Andrew JacksonTechnical Director IFFO 2 College YardLower Dagnall Street St Albans Hertfordshire AL3 4PAUnited KingdomTel +44 7808 783488 Email ajacksoniffonetWebiste wwwiffonet

First certification awarded under the IFFO Global Standard for Responsible Supply

ldquoThe first link in a fully certified aquaculture supply chain is in placerdquo

ldquoSupplies of ldquoIFFO-Assuredrdquo fishmeal and fish oil will shortly be on the marketrdquo

Jorge Mora (right) Operations Director of IFFO presenting the IFFO-Assured certificate to Mario Brescia (left) CEO of TASA at its headquarters in Lima Peru

10 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 11

Me r c a t o r M e d i a o r g a n i s e r o f t h e Offshore Mariculture

Conference 2010 is pleased to announce that the leading i ndus t r y a s soc i a t ions w i l l be suppor t i n g t he 2010 Conference

The European Aquaculture Techno logy amp I nnova t ion Platform (EATiP) the Federation o f Eu ropean Aquacu l tu re Producers (FEAP) the Global

Aquaculture All iance (GAA) and HGK Grupacija akvakul-ture the aquaculture affi l ia-tion of the Croatian Chamber of Economy (and member of FEAP) have all pledged their suppor t to the Conference that wi l l be held from the

June 16-18 2010 at the Hilton Imper ial Hotel Dubrovnik Croatia

Both EATiP (European Aquaculture Technology amp Innovation Platform) and FEAP (Federation of European Aquaculture Producers) are pleased to suppor t the Offshore Mariculture Conference s ince events such as these give fish farmers t h e o p p o r t u -n i t y t o l e a r n from both indus-t r i a l and RTD

exper ts about relevant technological advances to rece ive in formed updates on key research and policy issues and

to be able to network with col leagues from across the sector says Cour tney Hough general secretary of FEAP and EATiP

As wel l as promoting the event to their members and therefore rais ing awareness of the event and its presti-gious speakers it also means that members of any of the above assoc ia t ions wi l l be en t i t l ed to a 10 pe rcen t d i s count o f f the de lega te rate European Aquaculture Society members will also be ent i t led to the discount as the EAS have continued their suppor t f rom the prev ious even t he ld i n A l i c an te i n 2008

The technical conference will be held on the June 16 and 17 June with a visit to a fish farm on the June 18 2010 The Conference Networking Dinner will take place on the evening of Wednesday June 16 2010 at the fabulous Revelin For tress which was bui lt in 1462 to provide protection to the Eastern City Gate Place Over look ing the Old Ci ty Harbour the Revelins terrace offers what are probably the best views of the Old City and its famous Harbour

Mo re in fo rMatio n

Offshore MaricultureTel +44 1622 820622Website wwwoffshoremariculturecom

Industry support strengthens for 2010 OSM Conference

The full programme will be released shortly sessions will include

bull Policy legal framework and key research updates

bull Practical offshore farming operation

bull Spatial planning and moni-toring

bull Spotlight on Croatiabull Ecological monitoring and

future technologiesbull European and International

case studies

iN THE NEWSYour news could feature in our pages

Do you have an news item that you would like to see in our pages Send your press releases to the Production Manager - Nicky BarnesEmail nickybaquafeedcouk

10 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 11

Aqua News

The aquaculture industry today faces a number of challenges that threaten to restrict its development

and profitability

To live in harmony with consumer expec-tations of the highest possible standards of product quality and to do so in a sustainable and environmentally responsible manner necessitates an innovative coordinated and holistic approach within the aquaculture industry

The requirement for sustainability has focused on the composition of the feed being used as one of the key parameters determining the sustainability of production of a particular species particularly the fish-meal and fish oil content and how it relates to overall feed conversion ratios

Further formulation trends are moving towards low fishmeal and oil content in the grower feeds and finishing feeds designed to restore flesh quality parameters such as omega 3 fats and fillet quality that are con-sidered essential for the promotion of the product and its benefits for human health

This strategy addresses to some extent the limited resources of fishmeal and fish oil

Expectations of sustainabilityThe only way to achieve the expecta-

tions of sustainability and replace or supple-ment fishmeal and fish oil in aquafeeds is to exploit alternative protein and lipid sources

The obvious and traditionally used alter-natives are animal and plant protein sources

However in Europe and now increas-ingly in North America consumers pre-fer aquaculture products to have been

fed without the use of animal by-products due in part to recent health scares in the terrestrial animal sec-tor such as bovine spongiform encepha-lopathy (BSE)

The search is on therefore for not only the simple replacement of protein but the provi-sion of functional nutri-tion addressing both the sustainability and flesh quality requirements of the industry while

meeting the nutritional requirement of the animal being cultured

Alternative vegetable protein sources

Over the last two decades aquaculture research has focused on plant protein sources such as grains pulses and oilseeds as they are generally widely available

However the use of soybean meal and other plant based protein sources such as legume seeds oilseed cakes leaf meals leaf protein concentrates and root tuber meals have been limited due to the presence of a wide variety of anti-nutritional substances in these products and their interaction with the physiology of the animal resulting in depressed growth and feed efficiency mortality and abnormal intestinal morphol-ogy and damage

These substances act in different ways by affecting protein utilisation and digestion (eg protease inhibitors tannins lectins) by affecting mineral utilisation (eg phytate gossypol pigments glucosinolates) antivita-mins and other miscellaneous substances (eg phytoestrogens)

The increasing use of plant protein sources is also raising new concerns about possible contamination by mould derived mycotoxins

This risk is enhanced by the global nature and trading of plant protein sourc-es and the as yet little investigated impact of different mycotoxins and their interac-tions in aquaculture species In 2008 the Rapid Alert System for Food and Feed in

Protein sources Alternative protein sources should also show functionality if they are to play a role in modern aquafeedsby Keith Filer PhD research Manager Asia-Pacific biosciences Centre Alltech inc

Figure 1 A comparison of typical essential amino acid (EAA) requirements for fish and the EAA profile of AquateTM

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

juns

wor

th |

Dre

amst

ime

com

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

040

120

08

180

120

08

010

220

08

150

220

08

290

220

08

140

320

08

280

320

08

110

420

08

250

420

08

090

520

08

230

520

08

16 | InternatIonal AquAFeed | March-april 2010

Alltechreg has developed a range of products to address dietary requirements at different

stages of life in aquaculture species These sustainable product solutions are proven to

support profitability in aquaculture operations as well as performance in fish and shrimp

in the Family

Give your fish and shrimp the Alltech Aqua Advantage Contact your Alltech representative today for more information

trade

aquasolutionsalltechcomwwwalltechcom

Ideally suited for fish and shrimp in any stage of life to maintain performance and to support independence from traditional aquaculture diet ingredients

Specifically designed for the juvenile stages of life in fish and shrimp to support good health uniform growth and survival

A natural affordable product created for salmon in all life stages to maintain good health and defense mechanisms against adverse aquatic elements present in the surrounding environment

trade

trade

Aquate Ad for IAF due Feb 19 2010V2indd 1 2192010 115722 AM

Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

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1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

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(UKpound15 per copy)

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Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

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Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

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Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

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EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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If you have a product or service that you want to advertise but we do not have a heading let us know

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

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from raw material processing mixing and extruding to drying Process expertise

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The solution behind the solution

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fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 12: March | April 10 - International Aquafeed

Tecnoloacutegica de Alimentos SA (TASA) of Peru is the first fishmeal and fish

oil producer to achieve cer ti-fication under the new IFFO Global Standard for Responsible Supply

The cer tification has been awarded to the Callao Nor te plant of TASA the worldrsquos largest fishmeal producer Earlier this week Jorge Mora Operations Director of IFFO presented the

IFFO-Assured certificate to Mario Brescia CEO of TASA at its head-quarters in Lima Peru

IFFO has been developing its RS Standard since early 2008 with the help of a Technical Advisory Committee comprising repre-sentatives from the whole aqua-culture value chain including fish farmers feed producers and retailers and also NGOs

This business-to-business inde-pendently-audited programme will enable IFFO members to demonstrate to their customers that they are offer ing pure marine ingredients manufactured from responsibly sourced and traceable raw materials Whole fish used must come from fish-eries managed under the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

ldquoSince October 2009 when we launched the programme we have had applications from 15 manufacturing companies in four major producing countries sourcing from 10 fisheriesrdquo says IFFO director general Jonathan Shepherd

ldquoWe anticipate that there will be substantial supplies of IFFO-Assured fishmeal and fish oil on the market within months and that up to one third of global

p r o d u c t i o n could be certi-fied by the end of 2010rdquo

ldquoWith th i s p r o g r a m m e IFFO has put in place the first link in a fully certified supply chain for aqua-culture and for land animals such as pigs and poul tr y

It will help the industry demon-strate its commitment to respon-sible manufacture of fishmeal and fish oil as well as to the FAO Code of Conductrdquo said Dr Shepherd

Applicant factories which can demonstrate that they are ldquooper-ating a well managed system that ensures their marine raw mate-rials are responsibly sourced and processed to the IFFO Standardrdquo will be awarded certification

They will be able to offer IFFO-Assured fishmeal and fish oil and have the right to display the RS logo An up-to-date list of cur-rently certified fishmeal plants will be posted on the IFFO web site alongside the full Standard

Characteristics of the IFFO Global Standard for Responsible Supply (RS)

bull Standards set by a multi-stake-holder Technical Advisory

Committee including pro-ducers traders fish farmers feed manufacturers proc-essors retailers standard setting organisations and environmental NGOs The Assessment Management and Certification Process are contracted to Global Trust

bull A programme independ-ently administered by an ISO 651996 accredited certifica-tion body which is a member of the International Accreditation Forum (IFA)

bull I t d e m o n s t r a t e s responsible sourcing o f t r aceable r aw material and respon-sible production to ensure feed safety

bull A business-to-busi-ness tool designed to enable fishmeal and fish oil producers to demonstrate the integr i ty o f the i r products and their processes to their cus-tomers in the value-chain rather than a consumer eco-label

bull Whole fish used must come from fisheries that have been independently assessed and meet the key elements of the UN Food and Agriculture Organisation (FAO) Code of Conduct for Responsible Fisheries

bull Exclusion of illegal unre-por ted and unregulated (IUU) fish

bull The factor y must have attained Cer tification to the Internat ional Feed Ingredients Standard (IFIS) of the International Feed Safety Alliance (IFSA) or equiva-lent as proof of responsible manufacturing and the appli-cant must be in possession

of all relevant permits and licenses for the production and sale of fishmeal and fish oil products

bull This programme is com-patible with ISO 651996 (General requirements for bodies operating product cer tification systems) and with EN45011

bull Recogn i ses other cer-t i f i c a t ion p rog r ammes w h i c h h av e d e m o n -

strated equivalency and which are accepted within the industry - eg Marine Stewardship Council (MSC) for the fishery certification element only

The IFFO programme will continue to be developed in 2010 addressing further aspects of responsible supply

Mo re in fo rMatio n

Andrew JacksonTechnical Director IFFO 2 College YardLower Dagnall Street St Albans Hertfordshire AL3 4PAUnited KingdomTel +44 7808 783488 Email ajacksoniffonetWebiste wwwiffonet

First certification awarded under the IFFO Global Standard for Responsible Supply

ldquoThe first link in a fully certified aquaculture supply chain is in placerdquo

ldquoSupplies of ldquoIFFO-Assuredrdquo fishmeal and fish oil will shortly be on the marketrdquo

Jorge Mora (right) Operations Director of IFFO presenting the IFFO-Assured certificate to Mario Brescia (left) CEO of TASA at its headquarters in Lima Peru

10 | InternatIonal AquAFeed | March-april 2010

Aqua News

March-april 2010 | InternatIonal AquAFeed | 11

Me r c a t o r M e d i a o r g a n i s e r o f t h e Offshore Mariculture

Conference 2010 is pleased to announce that the leading i ndus t r y a s soc i a t ions w i l l be suppor t i n g t he 2010 Conference

The European Aquaculture Techno logy amp I nnova t ion Platform (EATiP) the Federation o f Eu ropean Aquacu l tu re Producers (FEAP) the Global

Aquaculture All iance (GAA) and HGK Grupacija akvakul-ture the aquaculture affi l ia-tion of the Croatian Chamber of Economy (and member of FEAP) have all pledged their suppor t to the Conference that wi l l be held from the

June 16-18 2010 at the Hilton Imper ial Hotel Dubrovnik Croatia

Both EATiP (European Aquaculture Technology amp Innovation Platform) and FEAP (Federation of European Aquaculture Producers) are pleased to suppor t the Offshore Mariculture Conference s ince events such as these give fish farmers t h e o p p o r t u -n i t y t o l e a r n from both indus-t r i a l and RTD

exper ts about relevant technological advances to rece ive in formed updates on key research and policy issues and

to be able to network with col leagues from across the sector says Cour tney Hough general secretary of FEAP and EATiP

As wel l as promoting the event to their members and therefore rais ing awareness of the event and its presti-gious speakers it also means that members of any of the above assoc ia t ions wi l l be en t i t l ed to a 10 pe rcen t d i s count o f f the de lega te rate European Aquaculture Society members will also be ent i t led to the discount as the EAS have continued their suppor t f rom the prev ious even t he ld i n A l i c an te i n 2008

The technical conference will be held on the June 16 and 17 June with a visit to a fish farm on the June 18 2010 The Conference Networking Dinner will take place on the evening of Wednesday June 16 2010 at the fabulous Revelin For tress which was bui lt in 1462 to provide protection to the Eastern City Gate Place Over look ing the Old Ci ty Harbour the Revelins terrace offers what are probably the best views of the Old City and its famous Harbour

Mo re in fo rMatio n

Offshore MaricultureTel +44 1622 820622Website wwwoffshoremariculturecom

Industry support strengthens for 2010 OSM Conference

The full programme will be released shortly sessions will include

bull Policy legal framework and key research updates

bull Practical offshore farming operation

bull Spatial planning and moni-toring

bull Spotlight on Croatiabull Ecological monitoring and

future technologiesbull European and International

case studies

iN THE NEWSYour news could feature in our pages

Do you have an news item that you would like to see in our pages Send your press releases to the Production Manager - Nicky BarnesEmail nickybaquafeedcouk

10 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 11

Aqua News

The aquaculture industry today faces a number of challenges that threaten to restrict its development

and profitability

To live in harmony with consumer expec-tations of the highest possible standards of product quality and to do so in a sustainable and environmentally responsible manner necessitates an innovative coordinated and holistic approach within the aquaculture industry

The requirement for sustainability has focused on the composition of the feed being used as one of the key parameters determining the sustainability of production of a particular species particularly the fish-meal and fish oil content and how it relates to overall feed conversion ratios

Further formulation trends are moving towards low fishmeal and oil content in the grower feeds and finishing feeds designed to restore flesh quality parameters such as omega 3 fats and fillet quality that are con-sidered essential for the promotion of the product and its benefits for human health

This strategy addresses to some extent the limited resources of fishmeal and fish oil

Expectations of sustainabilityThe only way to achieve the expecta-

tions of sustainability and replace or supple-ment fishmeal and fish oil in aquafeeds is to exploit alternative protein and lipid sources

The obvious and traditionally used alter-natives are animal and plant protein sources

However in Europe and now increas-ingly in North America consumers pre-fer aquaculture products to have been

fed without the use of animal by-products due in part to recent health scares in the terrestrial animal sec-tor such as bovine spongiform encepha-lopathy (BSE)

The search is on therefore for not only the simple replacement of protein but the provi-sion of functional nutri-tion addressing both the sustainability and flesh quality requirements of the industry while

meeting the nutritional requirement of the animal being cultured

Alternative vegetable protein sources

Over the last two decades aquaculture research has focused on plant protein sources such as grains pulses and oilseeds as they are generally widely available

However the use of soybean meal and other plant based protein sources such as legume seeds oilseed cakes leaf meals leaf protein concentrates and root tuber meals have been limited due to the presence of a wide variety of anti-nutritional substances in these products and their interaction with the physiology of the animal resulting in depressed growth and feed efficiency mortality and abnormal intestinal morphol-ogy and damage

These substances act in different ways by affecting protein utilisation and digestion (eg protease inhibitors tannins lectins) by affecting mineral utilisation (eg phytate gossypol pigments glucosinolates) antivita-mins and other miscellaneous substances (eg phytoestrogens)

The increasing use of plant protein sources is also raising new concerns about possible contamination by mould derived mycotoxins

This risk is enhanced by the global nature and trading of plant protein sourc-es and the as yet little investigated impact of different mycotoxins and their interac-tions in aquaculture species In 2008 the Rapid Alert System for Food and Feed in

Protein sources Alternative protein sources should also show functionality if they are to play a role in modern aquafeedsby Keith Filer PhD research Manager Asia-Pacific biosciences Centre Alltech inc

Figure 1 A comparison of typical essential amino acid (EAA) requirements for fish and the EAA profile of AquateTM

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

juns

wor

th |

Dre

amst

ime

com

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

040

120

08

180

120

08

010

220

08

150

220

08

290

220

08

140

320

08

280

320

08

110

420

08

250

420

08

090

520

08

230

520

08

16 | InternatIonal AquAFeed | March-april 2010

Alltechreg has developed a range of products to address dietary requirements at different

stages of life in aquaculture species These sustainable product solutions are proven to

support profitability in aquaculture operations as well as performance in fish and shrimp

in the Family

Give your fish and shrimp the Alltech Aqua Advantage Contact your Alltech representative today for more information

trade

aquasolutionsalltechcomwwwalltechcom

Ideally suited for fish and shrimp in any stage of life to maintain performance and to support independence from traditional aquaculture diet ingredients

Specifically designed for the juvenile stages of life in fish and shrimp to support good health uniform growth and survival

A natural affordable product created for salmon in all life stages to maintain good health and defense mechanisms against adverse aquatic elements present in the surrounding environment

trade

trade

Aquate Ad for IAF due Feb 19 2010V2indd 1 2192010 115722 AM

Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

8 page full colour document - 100 copies = UKpound489 (please add UKpound15 for every additional 100 copies)

Delivery charged on a case by case basis

For more details please visit wwwaquafeedcoukreprints

1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

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________________________________________________________

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Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

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19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

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5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

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W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

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6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

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International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

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Date 10 February 2010 212628 GMT

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International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

Tel Fax

Mobile

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Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 13: March | April 10 - International Aquafeed

Me r c a t o r M e d i a o r g a n i s e r o f t h e Offshore Mariculture

Conference 2010 is pleased to announce that the leading i ndus t r y a s soc i a t ions w i l l be suppor t i n g t he 2010 Conference

The European Aquaculture Techno logy amp I nnova t ion Platform (EATiP) the Federation o f Eu ropean Aquacu l tu re Producers (FEAP) the Global

Aquaculture All iance (GAA) and HGK Grupacija akvakul-ture the aquaculture affi l ia-tion of the Croatian Chamber of Economy (and member of FEAP) have all pledged their suppor t to the Conference that wi l l be held from the

June 16-18 2010 at the Hilton Imper ial Hotel Dubrovnik Croatia

Both EATiP (European Aquaculture Technology amp Innovation Platform) and FEAP (Federation of European Aquaculture Producers) are pleased to suppor t the Offshore Mariculture Conference s ince events such as these give fish farmers t h e o p p o r t u -n i t y t o l e a r n from both indus-t r i a l and RTD

exper ts about relevant technological advances to rece ive in formed updates on key research and policy issues and

to be able to network with col leagues from across the sector says Cour tney Hough general secretary of FEAP and EATiP

As wel l as promoting the event to their members and therefore rais ing awareness of the event and its presti-gious speakers it also means that members of any of the above assoc ia t ions wi l l be en t i t l ed to a 10 pe rcen t d i s count o f f the de lega te rate European Aquaculture Society members will also be ent i t led to the discount as the EAS have continued their suppor t f rom the prev ious even t he ld i n A l i c an te i n 2008

The technical conference will be held on the June 16 and 17 June with a visit to a fish farm on the June 18 2010 The Conference Networking Dinner will take place on the evening of Wednesday June 16 2010 at the fabulous Revelin For tress which was bui lt in 1462 to provide protection to the Eastern City Gate Place Over look ing the Old Ci ty Harbour the Revelins terrace offers what are probably the best views of the Old City and its famous Harbour

Mo re in fo rMatio n

Offshore MaricultureTel +44 1622 820622Website wwwoffshoremariculturecom

Industry support strengthens for 2010 OSM Conference

The full programme will be released shortly sessions will include

bull Policy legal framework and key research updates

bull Practical offshore farming operation

bull Spatial planning and moni-toring

bull Spotlight on Croatiabull Ecological monitoring and

future technologiesbull European and International

case studies

iN THE NEWSYour news could feature in our pages

Do you have an news item that you would like to see in our pages Send your press releases to the Production Manager - Nicky BarnesEmail nickybaquafeedcouk

10 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 11

Aqua News

The aquaculture industry today faces a number of challenges that threaten to restrict its development

and profitability

To live in harmony with consumer expec-tations of the highest possible standards of product quality and to do so in a sustainable and environmentally responsible manner necessitates an innovative coordinated and holistic approach within the aquaculture industry

The requirement for sustainability has focused on the composition of the feed being used as one of the key parameters determining the sustainability of production of a particular species particularly the fish-meal and fish oil content and how it relates to overall feed conversion ratios

Further formulation trends are moving towards low fishmeal and oil content in the grower feeds and finishing feeds designed to restore flesh quality parameters such as omega 3 fats and fillet quality that are con-sidered essential for the promotion of the product and its benefits for human health

This strategy addresses to some extent the limited resources of fishmeal and fish oil

Expectations of sustainabilityThe only way to achieve the expecta-

tions of sustainability and replace or supple-ment fishmeal and fish oil in aquafeeds is to exploit alternative protein and lipid sources

The obvious and traditionally used alter-natives are animal and plant protein sources

However in Europe and now increas-ingly in North America consumers pre-fer aquaculture products to have been

fed without the use of animal by-products due in part to recent health scares in the terrestrial animal sec-tor such as bovine spongiform encepha-lopathy (BSE)

The search is on therefore for not only the simple replacement of protein but the provi-sion of functional nutri-tion addressing both the sustainability and flesh quality requirements of the industry while

meeting the nutritional requirement of the animal being cultured

Alternative vegetable protein sources

Over the last two decades aquaculture research has focused on plant protein sources such as grains pulses and oilseeds as they are generally widely available

However the use of soybean meal and other plant based protein sources such as legume seeds oilseed cakes leaf meals leaf protein concentrates and root tuber meals have been limited due to the presence of a wide variety of anti-nutritional substances in these products and their interaction with the physiology of the animal resulting in depressed growth and feed efficiency mortality and abnormal intestinal morphol-ogy and damage

These substances act in different ways by affecting protein utilisation and digestion (eg protease inhibitors tannins lectins) by affecting mineral utilisation (eg phytate gossypol pigments glucosinolates) antivita-mins and other miscellaneous substances (eg phytoestrogens)

The increasing use of plant protein sources is also raising new concerns about possible contamination by mould derived mycotoxins

This risk is enhanced by the global nature and trading of plant protein sourc-es and the as yet little investigated impact of different mycotoxins and their interac-tions in aquaculture species In 2008 the Rapid Alert System for Food and Feed in

Protein sources Alternative protein sources should also show functionality if they are to play a role in modern aquafeedsby Keith Filer PhD research Manager Asia-Pacific biosciences Centre Alltech inc

Figure 1 A comparison of typical essential amino acid (EAA) requirements for fish and the EAA profile of AquateTM

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

juns

wor

th |

Dre

amst

ime

com

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

040

120

08

180

120

08

010

220

08

150

220

08

290

220

08

140

320

08

280

320

08

110

420

08

250

420

08

090

520

08

230

520

08

16 | InternatIonal AquAFeed | March-april 2010

Alltechreg has developed a range of products to address dietary requirements at different

stages of life in aquaculture species These sustainable product solutions are proven to

support profitability in aquaculture operations as well as performance in fish and shrimp

in the Family

Give your fish and shrimp the Alltech Aqua Advantage Contact your Alltech representative today for more information

trade

aquasolutionsalltechcomwwwalltechcom

Ideally suited for fish and shrimp in any stage of life to maintain performance and to support independence from traditional aquaculture diet ingredients

Specifically designed for the juvenile stages of life in fish and shrimp to support good health uniform growth and survival

A natural affordable product created for salmon in all life stages to maintain good health and defense mechanisms against adverse aquatic elements present in the surrounding environment

trade

trade

Aquate Ad for IAF due Feb 19 2010V2indd 1 2192010 115722 AM

Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

8 page full colour document - 100 copies = UKpound489 (please add UKpound15 for every additional 100 copies)

Delivery charged on a case by case basis

For more details please visit wwwaquafeedcoukreprints

1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

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Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

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16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

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19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

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16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

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5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

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6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

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International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

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International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

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Mobile

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Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

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If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 14: March | April 10 - International Aquafeed

The aquaculture industry today faces a number of challenges that threaten to restrict its development

and profitability

To live in harmony with consumer expec-tations of the highest possible standards of product quality and to do so in a sustainable and environmentally responsible manner necessitates an innovative coordinated and holistic approach within the aquaculture industry

The requirement for sustainability has focused on the composition of the feed being used as one of the key parameters determining the sustainability of production of a particular species particularly the fish-meal and fish oil content and how it relates to overall feed conversion ratios

Further formulation trends are moving towards low fishmeal and oil content in the grower feeds and finishing feeds designed to restore flesh quality parameters such as omega 3 fats and fillet quality that are con-sidered essential for the promotion of the product and its benefits for human health

This strategy addresses to some extent the limited resources of fishmeal and fish oil

Expectations of sustainabilityThe only way to achieve the expecta-

tions of sustainability and replace or supple-ment fishmeal and fish oil in aquafeeds is to exploit alternative protein and lipid sources

The obvious and traditionally used alter-natives are animal and plant protein sources

However in Europe and now increas-ingly in North America consumers pre-fer aquaculture products to have been

fed without the use of animal by-products due in part to recent health scares in the terrestrial animal sec-tor such as bovine spongiform encepha-lopathy (BSE)

The search is on therefore for not only the simple replacement of protein but the provi-sion of functional nutri-tion addressing both the sustainability and flesh quality requirements of the industry while

meeting the nutritional requirement of the animal being cultured

Alternative vegetable protein sources

Over the last two decades aquaculture research has focused on plant protein sources such as grains pulses and oilseeds as they are generally widely available

However the use of soybean meal and other plant based protein sources such as legume seeds oilseed cakes leaf meals leaf protein concentrates and root tuber meals have been limited due to the presence of a wide variety of anti-nutritional substances in these products and their interaction with the physiology of the animal resulting in depressed growth and feed efficiency mortality and abnormal intestinal morphol-ogy and damage

These substances act in different ways by affecting protein utilisation and digestion (eg protease inhibitors tannins lectins) by affecting mineral utilisation (eg phytate gossypol pigments glucosinolates) antivita-mins and other miscellaneous substances (eg phytoestrogens)

The increasing use of plant protein sources is also raising new concerns about possible contamination by mould derived mycotoxins

This risk is enhanced by the global nature and trading of plant protein sourc-es and the as yet little investigated impact of different mycotoxins and their interac-tions in aquaculture species In 2008 the Rapid Alert System for Food and Feed in

Protein sources Alternative protein sources should also show functionality if they are to play a role in modern aquafeedsby Keith Filer PhD research Manager Asia-Pacific biosciences Centre Alltech inc

Figure 1 A comparison of typical essential amino acid (EAA) requirements for fish and the EAA profile of AquateTM

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

juns

wor

th |

Dre

amst

ime

com

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

040

120

08

180

120

08

010

220

08

150

220

08

290

220

08

140

320

08

280

320

08

110

420

08

250

420

08

090

520

08

230

520

08

16 | InternatIonal AquAFeed | March-april 2010

Alltechreg has developed a range of products to address dietary requirements at different

stages of life in aquaculture species These sustainable product solutions are proven to

support profitability in aquaculture operations as well as performance in fish and shrimp

in the Family

Give your fish and shrimp the Alltech Aqua Advantage Contact your Alltech representative today for more information

trade

aquasolutionsalltechcomwwwalltechcom

Ideally suited for fish and shrimp in any stage of life to maintain performance and to support independence from traditional aquaculture diet ingredients

Specifically designed for the juvenile stages of life in fish and shrimp to support good health uniform growth and survival

A natural affordable product created for salmon in all life stages to maintain good health and defense mechanisms against adverse aquatic elements present in the surrounding environment

trade

trade

Aquate Ad for IAF due Feb 19 2010V2indd 1 2192010 115722 AM

Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

8 page full colour document - 100 copies = UKpound489 (please add UKpound15 for every additional 100 copies)

Delivery charged on a case by case basis

For more details please visit wwwaquafeedcoukreprints

1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

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21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

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San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

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________________________________________________________

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20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

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E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

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19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

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5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

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8th - 10th July 10Indo Livestock 2010

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6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

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Date 10 February 2010 212628 GMT

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International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

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DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

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THE 5TH INDONESIArsquoS NO1

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 15: March | April 10 - International Aquafeed

the EU noted that mycotoxins accounted for 56 percent of border rejections when identi-fied by risk This concern may become increasingly important in aquafeed formulations in the future

A number of production tech-niques and specific products are used to help reduce the effect of anti-nutritional substances and mycotoxins in terrestrial animal production but these are not widely used in the aquaculture sector at this time Studies using enzymes and mycotoxin adsorb-ents are being carried out to find alterna-tives to overcome these limitations

Single-celled proteinsSingle-celled proteins such as micro-

algae bacteria and yeast are now being considered as attractive alternatives to the more traditional plant protein sources Many of these have been incorporated into aquafeed with differing degrees of success (Oliva-Teles and Goncalves 2001)

Fermentation technologies may be used to produce sustainable sources of single-celled proteins algae and yeast being two examples

Yeast based proteins have been the most thoroughly investigated as protein sources and as a dietary replacement for fishmeal because they are a rich source of protein with a relatively good balance of essential amino acids They contain B complex vita-mins pigments complex carbohydrates and

are a source of dietary nucleotides which have been shown to promote growth and enhance non-specific immune activity They are also low in phosphorous which can lead to less water and environmental contamina-tion than fishmeal and other plant based alternative protein sources that contain high levels of this mineral

The processing and extraction tech-nologies of both cell wall material and yeast extract are critical in providing function-

The primary defence mechanism of a fish can be considered to be the physical barrier (skin gills and gastrointestinal tract) Both externally and internally therefore

the mucosal barrier and tissue cellular membrane integrity play a vital role copy M

juns

wor

th |

Dre

amst

ime

com

12 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 13

F Protein

have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

040

120

08

180

120

08

010

220

08

150

220

08

290

220

08

140

320

08

280

320

08

110

420

08

250

420

08

090

520

08

230

520

08

16 | InternatIonal AquAFeed | March-april 2010

Alltechreg has developed a range of products to address dietary requirements at different

stages of life in aquaculture species These sustainable product solutions are proven to

support profitability in aquaculture operations as well as performance in fish and shrimp

in the Family

Give your fish and shrimp the Alltech Aqua Advantage Contact your Alltech representative today for more information

trade

aquasolutionsalltechcomwwwalltechcom

Ideally suited for fish and shrimp in any stage of life to maintain performance and to support independence from traditional aquaculture diet ingredients

Specifically designed for the juvenile stages of life in fish and shrimp to support good health uniform growth and survival

A natural affordable product created for salmon in all life stages to maintain good health and defense mechanisms against adverse aquatic elements present in the surrounding environment

trade

trade

Aquate Ad for IAF due Feb 19 2010V2indd 1 2192010 115722 AM

Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

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1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

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Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

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DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

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THE 5TH INDONESIArsquoS NO1

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

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BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 16: March | April 10 - International Aquafeed

have a requirement for a well-balanced mixture of essential and non-essential amino acids from which to construct their own protein tissues and therefore the balance of essential amino acids in the diet is crucial Aquate is a rich source of highly digestible amino acids with a crude protein content of approximately 47 percent

The amino acid profile of Aquate closely matches the requirement for fish (Figure 1) and contains docosahexaenoic acid (DHA) at approximately 338 percent of its fat content Furthermore its nucle-otide content provides functional nutri-ents that are present in the yeast extract component Nucleotides are known to play a major role in almost all biologi-cal processes and this includes storage of energy components of several coen-zymes which are involved in carbohydrate protein and fat metabolism mediation in cellular processes control of several enzymatic reactions and intermediates in biosynthetic reactions

Aquatersquos level of nucleotides ranges from five percent to as much as 15 percent in the specialised AquateTM Junior product Aquate Junior has been designed for juve-nile stages of aquaculture species where a rich source of nucleotides are especially important for immunity and gut health

The Aquate family has been designed to provide functionality in aquafeeds to meet the specific requirements of a particular aquaculture sector or species AquateTM SPMP also contains the specific yeast cell wall component Bio-Mosreg which has been used to provide an effec-tive feed component in the fight to control sea lice in salmonid culture Combinations of functional nutrition in this manner has resulted in improve-ments in gut morphology nutrient uptake improved immuno-competence and disease resistance

and feed industry or for the extrac-tion of high-valued components such as vitamins (C amp D2) n-fatty acids pigments and antioxidants (B carotene astaxanthin lutein)

Through the careful formulation and blending of algal and yeast components Alltech has produced the AquateTM range of products designed to provide nutritional solutions for the aquaculture industry

Aquate is the commercial name for a second generation of sustainable product solutions aimed at enhancing biomass production feed efficiency and boosting natural defence mechanisms in aquacul-ture species so encouraging healthier and more robust populations

Aquaculture species like other animals

ality and optimising nutritional potential by ensuring a high degree of digestibil-ity Industrial spray drying techniques have played a significant role in improving these characteristics

Microalgae are rich sources of protein carbohydrates and lipids

The amino acid profile of almost all algae compares favourably to other food protein sources and the carbohydrate components are highly digestible

However it is the lipid content that is especially interesting because of the func-tionality of this component particularly so in some species rich in n-3 and n-6 fatty acids

Today commercial microalgae produc-tion is used to produce algal biomass for direct use as feed additives in the food

Table 1 the number of sea lice found to be present on sampled juvenile salmon

Control CAGES BIo-MoS CAGES

1 2 3 4 AVERAGE 1 2 3 4 AVERAGE T-TEST

Maturefemales 1 2 0 1 1 0 0 1 1 05 0178

Preadult 14 32 24 53 3075 29 24 15 20 22 0179

Chalimus 22 4 8 26 15 8 8 14 7 925 0170

Caligus 17 14 14 5 125 8 14 7 5 85 0140

TOTAL 54 52 46 85 5925 45 46 37 34 405 0044

Figure 2 Mucal production increases with the addition of Bio-Mosreg (Torrecillas et al 2008)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

040

120

08

180

120

08

010

220

08

150

220

08

290

220

08

140

320

08

280

320

08

110

420

08

250

420

08

090

520

08

230

520

08

16 | InternatIonal AquAFeed | March-april 2010

Alltechreg has developed a range of products to address dietary requirements at different

stages of life in aquaculture species These sustainable product solutions are proven to

support profitability in aquaculture operations as well as performance in fish and shrimp

in the Family

Give your fish and shrimp the Alltech Aqua Advantage Contact your Alltech representative today for more information

trade

aquasolutionsalltechcomwwwalltechcom

Ideally suited for fish and shrimp in any stage of life to maintain performance and to support independence from traditional aquaculture diet ingredients

Specifically designed for the juvenile stages of life in fish and shrimp to support good health uniform growth and survival

A natural affordable product created for salmon in all life stages to maintain good health and defense mechanisms against adverse aquatic elements present in the surrounding environment

trade

trade

Aquate Ad for IAF due Feb 19 2010V2indd 1 2192010 115722 AM

Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

8 page full colour document - 100 copies = UKpound489 (please add UKpound15 for every additional 100 copies)

Delivery charged on a case by case basis

For more details please visit wwwaquafeedcoukreprints

1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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IAF1001

Buy a paper copy ofthis issue

(UKpound15 per copy)

Subscribe to IAFSubscribers to IAF receive 5 issues

per year plus a copy of theInternational Aquafeed Directory amp

Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

You want to know more about our event Then please visit wwwsummit2010org

Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

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Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

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I ND U S

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Media Partners

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

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BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 17: March | April 10 - International Aquafeed

retained in the fish flesh so indicating more effective treatment

The combined effect of SPMP and improved operational practises resulted in reduced Chalimi resettlement follow-ing treatment which was attributed to increased mucal production (Figure 5) and a general overall lower level of pressure from all stages of total mobile lice

Marine Harvest concluded that the Loch Sunart trials indicated that bull The SPMP diet produced more epi-

dermal mucus than the control dietbull The SPMP diets promoted better gut

integrity which allowed better uptake of SLICEreg into the flesh of fish

bull Using SPMP to enhance emamectin uptake was environmentally positive

bull Using SPMP to encourage epidermal mucus curbed Chalimi resettlement in comparison with control populations

bull SPMP can be incorporated into the diet at the initial stage of food produc-tion thus eliminating an additional cost for top coating

In Norway Salmon Group following industrial trials using SPMP concluded that

by the presence of bioactive substances and the epidermal migration of inflam-matory cells and their secretions may affect the establish-ment and prolifera-tion of ectoparasitic copepods ciliates or monogenean (Jones 2001)

Work at GIFAS monitored sea

lice development on juvenile salmon and showed that after a seven week period of feeding the total sea lice counts showed that fish fed with the addition of Bio-Mos had significantly lower overall numbers of sea lice present compared with the fish fed the control diet In addition the total number of individual salmon infected by sea lice (irrespective of species life stage or sex) was significantly reduced by the Bio-Mos supplementation (Table 1)

In the United Kingdom the aim of the National Sea Lice Strategy is to extend the lice free period and reduce the frequency of therapeutic intervention

M a r i n e Harvest sam-pled fish muscle for emamectin b e n z o a t e (EmB) in fish fed Aquate SPMP and the control diets i m m e d i a t e l y after the end of medication with SLICEreg N o t i c e a b l y higher levels of the medicinal residue were found in the fish fed Aquate SPMP (Figure 4) This is important envi-ronmentally as less emamec-tin benzoate is voided in the faeces and more is

AquateTM

SPMP and Atlantic salmon

The primary defence mechanism of a fish exposed to an infectious or damaging agent can be considered to be the physical barrier (skin gills and gastrointestinal tract) and their protection mechanisms at the point of interaction of the environment and the physiology of the fish Both externally and internally therefore the mucosal bar-rier and tissue cellular membrane integrity play a vital role in this process

In sea bass (Dicentrarchus labrax) mucosal production increases when Bio-Mos is fed as indicated by the significant increase (Plt005) in the number of cells secreting acid mucin in the posterior gut (Figure 2) The quantity of lysozyme in the mucus was also increased

In commercial field studies carried out by Marine Harvest in 2008 (Wallace et al 2009) salmon fed on Aquate SPMP showed increased skin mucous secretion (Figure 3) This increased skin mucosa was character-ised in the field by noticeably more slimy fish and was also widely reported by site managers from Salmon Group in Norway

The role of mucus in anti-parasitic mechanisms in fish is not well under-stood Buchmann and Bresciani (1998) demonstrated that mucus as measured by the density of mucous cells could play a role in limiting the parasite load of monogeans in rainbow trout Other authors have shown that monogenean and crustacean ectoparasites can modu-late mucus production during attach-ment by reducing the mucous cells in the skin of the host (Wells amp Cone 1990 Nolan et al 1999)

Recent studies however have begun to clarify the relative roles of the immune system in fighting parasitic infections in fish It has been suggested that the physico-chemical characteristics of skin mucus as determined

Figure 3 Typical skin mucus development on salmon fed AquateTM SPMP in Scotland (Wallace et al 2009)

14 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 15

F Protein

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

040

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08

010

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16 | InternatIonal AquAFeed | March-april 2010

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Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

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20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

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of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

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PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

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PROFILE Technology

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Technical SpecificationInternational Aquafeed magazine feature article reprints

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bull For use as Point of sale materials exhibition handouts etc

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Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

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Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

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________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

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E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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(UKpound15 per copy)

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Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

You want to know more about our event Then please visit wwwsummit2010org

Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

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EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

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Y

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MY

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idl10 - Grain Feedai 1222010 45438 PM

40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

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Y

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Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

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bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

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Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

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Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

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SpotMark - wwwsptmrkcom

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Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

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from raw material processing mixing and extruding to drying Process expertise

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The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 18: March | April 10 - International Aquafeed

conversion ratio led Virginia Cobia Farms to consider the inclusion of Aquate in their proprietary cobia grow-out formulations

Conclusion Single-celled protein sources have the

potential to become a renewable source of functional nutrition providing both alterna-tives to fishmeal and fish oil

ReferencesBuchmann K amp Bresciani J (1998) Microenvironment of Gyrodactylus derjavini on rainbow trout Oncorhynchus mykiss association between mucous cell density on skin and site selection Parisitology Research 84 17-24

Chou RL Her BY Su MS Hwang G Wu YH amp Chen HY (2004) Substituting fishmeal with soybean meal in diets of juvenile cobia Rachycentron canadum Aquaculture 229 325-333

Jones SRM (2001) The occurrence and mechanisms of innate immunity against parasites in fish Developmental and Comparative Immunology 25 841-852

Nolan DT Reilly P amp Wendelaar Bonga SE (1999) Infection with low numbers of sea louse Lepeoptheirus salmonis induces stress-related effects in post smolt Atlantic salmon (Salmo salar) Can J Fish Aquatic Sci 56 947-959

Oliva-Teles A amp P Goncalves 2001 Partial replacement of fishmeal by brewers yeast (Saccaromyces cerevisiae) in diets for sea bass (Dicentrarchus labrax) juveniles Aquaculture 202 269-278

Torrecillas S Makol A Caballero MJ Montero D Sweetman J amp Izquierdo MS (2008) Enhanced nutrient utilization and bacterial infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides Poster presented at XIII ISFNF Meeting Brazil 1-5 June

Wallace C Waddell R Cockerill D amp Ritchie G (2009) Double mucal effect boosts sea lice control and potentially extends useful life of medications Fish Farming Xpert No 109 48-53

Wells PR amp Cone DK (1990) Experimental studies on the effect of Gyrodactylus colemanensis and G salmonis on density of mucous cells in the epidermis of fry of Oncorhynchus mykiss J Fish Biol 37 599-603

canadum) Chou et al 2004 reported that a 40 percent replacement of fishmeal with soybean meal did not have a neg-ative effect on growth performance but that at higher replacement rates growth perform-ance decreased

A recent trial at Virginia Cobia Farms in Saltville Virginia USA investigated the poten-tial of Aquate inclusion (30 percent of the dry diet) to replace fish-meal and fish oil for the marine carnivore cobia Racyhycentron canadum

The high level of replacement of fishmeal was designed to capital-ise on the DHA content of Aquate and investigate if it could not only satisfy the essential fatty acid requirements of juvenile cobia but also provide adequate n-3 HUFA in the final product for human consumers

A commercial diet regularly used for cobia production was used as the control diet and a second experimental diet high in soy product inclusion (gt55 percent of the dry diet) was also included At the end of the feeding trial the fish were harvested and utilized for test market-ing purposes in which

chefs distributors and others compared the cobia fed the experimental diets in terms of taste texture physical and visual preferences

The trial showed that Aquate could be used as a partial provider of essential n-3 HUFA in marine fish diets and that the inclusion of Aquate appeared to impart beneficial taste and texture characteristics to the final product with 83 percent of the test panel prefer-ring the Aquate fed fish to that of the high soy inclusion diet This combined with an improved feed

bull 45 farms reported better general health status in the SPMP fed fish

bull 45 farms reported less lice treat-ments in the trial fish

bull All farms reported that the fish appeared to be very slimy

Aquate trial with cobiaIn omnivores and herbivorous species

such as catfish or tilapia high replacement levels (gt40 percent) of fishmeal can be tolerated provided that the amino acid requirement of the fish species is met from other sources However in the car-nivorous fish species cobia (Racyhycentron

Mo re in fo rMatio n

Keith Filer PhDResearch ManagerAsia-Pacific Biosciences CentreAlltech IncEmail kfileralltechcom

Figure 4 EmB residues in the SPMP study in Loch Sunart (Wallace et al 2009)

Single-celled protein sources have the potential to become a renewable source of functional nutrition providing both alternatives to fishmeal and fish oil

16 | InternatIonal AquAFeed | March-april 2010

F Protein

Figure 5 Average Chalimi levels from SPMP fed fish versus controls Loch Sunart (Wallace et al 2009)

040

120

08

180

120

08

010

220

08

150

220

08

290

220

08

140

320

08

280

320

08

110

420

08

250

420

08

090

520

08

230

520

08

16 | InternatIonal AquAFeed | March-april 2010

Alltechreg has developed a range of products to address dietary requirements at different

stages of life in aquaculture species These sustainable product solutions are proven to

support profitability in aquaculture operations as well as performance in fish and shrimp

in the Family

Give your fish and shrimp the Alltech Aqua Advantage Contact your Alltech representative today for more information

trade

aquasolutionsalltechcomwwwalltechcom

Ideally suited for fish and shrimp in any stage of life to maintain performance and to support independence from traditional aquaculture diet ingredients

Specifically designed for the juvenile stages of life in fish and shrimp to support good health uniform growth and survival

A natural affordable product created for salmon in all life stages to maintain good health and defense mechanisms against adverse aquatic elements present in the surrounding environment

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Aquate Ad for IAF due Feb 19 2010V2indd 1 2192010 115722 AM

Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

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Delivery charged on a case by case basis

For more details please visit wwwaquafeedcoukreprints

1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

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21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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________________________________________________________

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20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

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19th - 20th May 10 AQUACULTURE UK 2010

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23rd - 26th May 10 Australasian Aquaculture 2010

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T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

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16th - 18th June 10Offshore Mariculture Conference 2010

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5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

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T +34 93 2212955 | F +34 93 4592059

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International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

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Date 10 February 2010 212628 GMT

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International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

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DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

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Page 19: March | April 10 - International Aquafeed

16 | InternatIonal AquAFeed | March-april 2010

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Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

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20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

8 page full colour document - 100 copies = UKpound489 (please add UKpound15 for every additional 100 copies)

Delivery charged on a case by case basis

For more details please visit wwwaquafeedcoukreprints

1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

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Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

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________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

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E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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(UKpound15 per copy)

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per year plus a copy of theInternational Aquafeed Directory amp

Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

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EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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If you have a product or service that you want to advertise but we do not have a heading let us know

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MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

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Page 20: March | April 10 - International Aquafeed

Globally two million metric tonnes of prawn valued at US$144 billion were produced in 2004(1) with

large industry growth observed in Asian countries

Intensification of prawn production has resulted in an increase of bacterial infec-tions which frequently cause mass mortali-ties of prawn larvae due to the proliferation of opportunistic pathogenic bacteria

Disease is often the result of stress caused by poor water quality and by reduc-ing stress less mortality may occur

The single most critical factor governing optimal growth and survival of prawn is water quality Prawns are delicate creatures and can be stressed by adverse environ-mental conditions in culture ponds result-ing in reduced appetite slow growth as well as being more susceptible to disease (2)

Vibrio spp are normally present in the culture facilities larval gut flora of prawns

and in the live feed and they usually cause disease under sub-optimal culture condi-tions Therefore the successful completion of the larval cycle in prawns requires a healthy microbial balance in the system (3)

During the larval stages of prawn production luminescent vibriosis caused by Vibrio spp is particularly severe and sporadically causes temporary closure of economic activities in prawn larviculture facilities (3)

Vibriosis elicits signs of lethargy degener-ation of the gills lymphoid organs digestive glands and detachment of the epithelium lining the mid-gut all of which lead to even-tual or rapid mortality

Application of disinfectants and antimi-crobials can complete the larval cycle in prawns but has led to the emergence of antibiotic-resistant pathogens with threat of gene transfer to humans as well as waste antimicrobials ending up in the environment These negative consequences of antibiotic treatments have led to the development

of probiotics to manipulate the microbial balance (3)

Probiotics are organisms that create a favourable environment for the growth and well-being of the host animals by their transience through the gastrointestinal tract or merely by their presence in the water

The selection of probiotics for specific marine aquatic species has to be considered carefully in order to make them spe-cies specific (4) Probiotic candidates are most often bacteria with inhibitory activity against a target pathogen or the dominant intestinal bacterial flora in larvae from well performing prawn-rearing groups

For example Hill et al (5) identified Bacillus pumilis in the mid gut of P monodon which had an inhibitory effect against patho-genic bacteria The dominant bacterial flora is possibly capable of excluding invading bacteria from the adhesion sites of the gut wall thus supporting the endemic bacterial flora of prawn - a more natural approach to protecting prawns from diseases

This article focuses on our current knowledge of the benefits of probiotic application selecting probiotics and their modes of actions in combating microbial diseases in prawn production

Evaluation of probiotic bacteria in prawn productionby Tim Orsquobrine university of Plymouth united Kingdom

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

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1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

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Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

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IAN

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PAR T E

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DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

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THE 5TH INDONESIArsquoS NO1

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

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BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 21: March | April 10 - International Aquafeed

How should probiotics be administered

Probiotics could be used to replace chemicals and antibiotics and have already been shown to increase the immune response survival and disease resistance in prawns (6) Probiotics could either be incorporated into the feed or administered into the rearing water Hai et al (4) found that both feed and water administered application was efficient in the transfer of probiotics to the prawns However survival spe-cific growth rates and total haemocyte counts (THCs) concentrations were higher when probiotics were supple-mented through feed (4)

Thus feed incorporated probiotics are more beneficial than probiotics adminis-tered to the rearing water THCs play important roles in the crustacean immune response as the haemocytes encapsulate bacteria thus a high haemocyte count clears the bacterial load in the haemolymph

How do probiotics workProposed mode of actions of probiotics

includes immunostimulation inhibition or competition (antagonistic) with potential pathogens or simply maintenance of healthy and diverse intestinal microbiota

Non pathogenic strains of Vibrio Aeromonas Pseudomonas Bacillus and Alteromonas that are known to have antago-nistic properties to pathogenic strains can be used as probiotic strains (8) The inhibitory compounds produced from the antagonistic organisms can control the pathogenic bacteria population in a system without affecting the water quality and therefore could be very beneficial to prawn production

Examples of antagonistic bacteria that secrete inhibitory substances include nisin (antibacterial peptide) of Lactobacillus lactis pediocin (proteinaceous bacteriocin) of Pediococcus acidlactici sidephore-like mol-ecules and pyocyanin (antibiotic pigment) of Pseudomonas spp The primary require-ment for the demonstration of antagonistic mode of action of probiotics in aquaculture systems is the assurance of the minimum inhibitory concentration of the antagonistic compound in the culture which is supple-mented to the larval rearing tank (3)

Antagonistic gram-positive Bacillus spp have been used as a probiotic in fish and shellfish (9) and could be used in prawn aquaculture

Bacillus spp are naturally ingested by prawns and other organisms that feed in

or on the sediments The spores of Bacillus sp can be easily introduced into dry food and are unlikely to use genes for antibiotic resistance or virulence from the vibrios or related gram-negative bacteria (6) and have not been associated with aquatic organism pathologies

For this reason their use has been promoted and widely accept-ed within the industry Banerjee et al (9) observed 90 percent inhibi-tion of patho-genic bacteria by Bacil lus subtilis along with competi-tive exclusion of patho-genic vibr ios by Bacillus spp M o r i a r t y ( 6 ) reported that when Bacillus sp was added to pond water in penaeid prawn culture there was a decrease in luminous Vibrio densities

The antago-nistic factor of

Bacillus spp to inhibiting the growth of pathogenic bacteria could be one of the enzymes produced by Bacillus including protease amylase lipase and gelatinase (6)

The success of Bacillus spp has been demonstrated by the development and use

18 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 19

F Probiotic

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

8 page full colour document - 100 copies = UKpound489 (please add UKpound15 for every additional 100 copies)

Delivery charged on a case by case basis

For more details please visit wwwaquafeedcoukreprints

1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

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________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

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T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

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8th - 10th July 10Indo Livestock 2010

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From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

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International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

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Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

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Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

bull Crevetecbull Danisco Animal Nutritionbull Exquimbull Ge-Pro Aquabull Inve Nutri-Ad

Elevator buckets

Equipment for salebull A Foeth BVbull Condex (UK) Ltd

bull Filter Screen Supply Ltd

Extrudersbull Almex

bull Anderson Internationalbull Baker Perkinsbull Brabender

bull Caliber Controlbull Conforma Clad Incbull Coperionbull ESE Intecbull Fudex Group Spabull Insta Pro International

bull Setrembull Suehiro EPMbull TGC Extrusionbull Wenger

Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 22: March | April 10 - International Aquafeed

also reported that B coagulans used as a water additive at a certain concentration could sig-nificantly increase the survival rate and some digestive enzyme activities of prawn larvae In addition the use of B subtilis was improved prawn immune responses by increases in phe-noloxidase activity phagocytic activity and clearance efficiency against Vibrio alginolyticus

Several probiotics versus one probiotic

Combining probiotics to pro-duce a range of beneficial effects on prawn production has been studied and is often employed in commercial probiotics as previously mentioned by the use of multiple Bacillus spp in SANOLIFEreg MIC (10)

The combination of two pro-biotics in Western king prawn production was not found to significantly improve the specific growth rate of prawns compared to single use of a probiotic but the prawns exposed to the combined probiotics did elicit a lower proportion of hyalinocytes (involved in phagocytosis) as well as having a lower bacterial load in the haemolymph hence were

healthier than those exposed to the individual probiotics (4)

Far et al (15) also compared the use of one probiotic against multiple probiotics through feed incorporation Treatment groups consisted of prawns fed diet with B subtilis and prawns fed a diet mixed with B subtilis and a commercial probiotic (BIO-GOLD Korea) which con-sisted of L acidophilus Bacillus subtilis and L plantarum Interestingly diets just consisting of B subtilis had significantly higher survival and yield compared with the diet incorporating both B subtilis and BIO-GOLD Thus it appears that B subtilis alone out performs BIO-GOLD but it should also be noted that a diet just containing BIO-GOLD was not different from the diet containing B Subtilis and BIO-GOLD

Therefore an inhibitory action between the consortium of probiotics in the BIO-GOLD and B subtilis diet can be ruled out Overall though the addition of B subtilis was found to improve prawn (L vannamei) survival rate and yield (15)

super oxide radicals resulting in the inhibi-tion of bacteria due to oxidative damage and lipid peroxidation

As a result prawns fed the probiotics Pseudomonas synxantha and P aeruginosa had higher specific growth rates survival and food conversion ratio compared to prawns not fed probiotics (11) Probiotics stimulated the prawns to produce a higher THC and resulted in the lowest bacterial load in the haemolmpyh thus improving the health of the prawns (4)

Nakayama et al (12) demonstrated that B Subtilis could be effective in the inhibition of Vibrio growth while B Licheniformis and B Megaterium could be effective in reducing V harveyi haemolytic activities suggesting that compounds produced by Bacillus might be effective against Vibrio growth and toxin production

Balcazar and Rohas-Luna (13)reported that B subtilis produced antimicrobial activity against pathogenic Vibrio spp and that prawn mortality was reduced by treatment with B subtilis Zhou et al (14)

of SANOLIFEreg MIC a mixture of strains of Bacillus spp Decamp et al (10) reported on the performance of SANOLIFEreg MIC on Asian and Latin-American hatcheries with P monodon and L vannamei and found that SANOLIFEreg MIC performed as well as prophylactic use of antibiotics and was also a cost effective alternative

Pai et al (3) suggested another mode of action of Bacillus immunostimulation and enhanced food acceptance which results in improved general health of prawns rather than the antagonistic mode described above In their study they found that the bacteria that showed the greatest antagonism was the Pseudomonas bacteria

The Pseudomonas bacteria suppressed the vibrio population in a P monodon larval rearing system by production of pyocyanin giving them a competitive advantage over V harveyi mediated by the killing of the pathogen (3)

The unusual redox properties of pyo-cyanin and other phenazine compounds secreted by fluorescent Pseudomonads can enhance the production of hydroxide and

20 | InternatIonal AquAFeed | March-april 2010

F Probiotic

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

8 page full colour document - 100 copies = UKpound489 (please add UKpound15 for every additional 100 copies)

Delivery charged on a case by case basis

For more details please visit wwwaquafeedcoukreprints

1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

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Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

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________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

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E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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IAF1001

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(UKpound15 per copy)

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per year plus a copy of theInternational Aquafeed Directory amp

Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

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Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

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Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

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Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

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EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

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THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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Services (publications)

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To place a classified advert in our next issue and take

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WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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If you have a product or service that you want to advertise but we do not have a heading let us know

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MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

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Geelen Counterflow - wwwgeelencounterflowcom

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Marevent - wwwmareventcom

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Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

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from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 23: March | April 10 - International Aquafeed

20 | InternatIonal AquAFeed | March-april 2010

Dry with 20-50 less energy

Geelen Counterflow T +31-475-592315Geelen Counterflow USA Inc T +1-772-559-4338Geelen Counterflow Ameacuterica Latina T +54-9-2362-418899E infogeelencounterflowcom I wwwgeelencounterflowcom

adv 210x297 fc 08indd 1 13-05-2008 103457

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

8 page full colour document - 100 copies = UKpound489 (please add UKpound15 for every additional 100 copies)

Delivery charged on a case by case basis

For more details please visit wwwaquafeedcoukreprints

1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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IAF1001

Buy a paper copy ofthis issue

(UKpound15 per copy)

Subscribe to IAFSubscribers to IAF receive 5 issues

per year plus a copy of theInternational Aquafeed Directory amp

Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

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Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

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Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

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Media Partners

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

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THE 5TH INDONESIArsquoS NO1

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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Alltech Inc - wwwalltechcom

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

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BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 24: March | April 10 - International Aquafeed

of western king prawns (Penaeus latisulcatus Kishinouye 1896) Fish amp Shellfish Immunology 27(2)100-104

Hill JE Baiano JCF and Barnes AC 2009 Isolation of a novel strain of Bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens Journal of Fish Diseases 9999(9999)

Moriarty DJW 1998 Control of luminous Vibrio species in penaeid aquaculture ponds Aquaculture 164(1-4)351-358

Hai N Buller N and Fotedar R 2009 Effects of probiotics (Pseudomonas synxantha and P aeruginosa) on the growth survival and immune parameters of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture Research 40590-602

Moriatry D 2003 Probiotics in aquaculture Microbiology Australia 2415-17

Banerjee S Devaraja TN Shariff M and Yusoff FM 2007 Comparison of four antibiotics with indigenous marine Bacillus spp in controlling pathogenic bacteria from shrimp and artemia Journal of Fish Diseases 30(7)383-390

Decamp O Moriarty D and Lavens P 2008 Probiotics for shrimp larviculture review of field data from Asia and Latin America Aquaculture Research 39(4)334-338

Hai N and Fotedar R 2009 Comparison of the effects of the prebiotics (Bio-Mos and -1 3-D-glucan) and the customised probiotics (Pseudomonas synxantha and P aeruginosa) on the culture of juvenile western king prawns (Penaeus latisulcatus Kishinouye 1896) Aquaculture 289(3-4)310-316

Nakayama T Lu H and Nomura N 2009 Inhibitory effects of Bacillus probionts on growth and toxin production of Vibrio harveyi pathogens of shrimp Letters in Applied Microbiology 9999(999A)

Balcazar JL and Rojas-Luna T 2007 Inhibitory activity of probiotic Bacillus subtilis UTM 126 against vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei) Current Microbiology 55(5)409-412

Zhou X Wang Y and Li W 2009 Effect of probiotic on larvae shrimp (Penaeus vannamei) based on water quality survival rate and digestive enzyme activities Aquaculture 287(3-4)349-353

Far HZ Saad CRB Daud HM Harmin SA and Shakibazadeh S 2009 Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vannamei) African Journal of Biotechnology 8(14)3369-3376

Wang YB Xu ZR and Xia MS 2005 The effectiveness of commercial probiotics in northern white shrimp Penaeus vannamei ponds Fisheries Science 71(5)1036-1041

probiotics led to the market being flooded with commercial probiotics with little or no scientific basing (10)

Also probiotics are only beneficial and cost-effective when they are properly applied together with suitable farm manage-ment (10)

Other factors should also be inves-tigated to reduce disease outbreaks such as the use of prebiotics (Bio-Mos and β-13-D-glucan) which provide nutrients for selected bacteria to encourage their proliferation in the gut and have been found to improve survival and spe-cific growth rate of prawns (11)

Perhaps more importantly though as emphasised at the beginning of this article maintaining the water quality is important as this factor will dictate the level of stress on the prawns and good water qual-ity will result in healthier prawns with no addition of antimicrobials pro-

biotics or prebiotics needed ndash this is how a sustainable production system should attain to be

ReferencesFAO 2007 The State of World Fisheries and Aquaculture 2006 Rome

Boyd CE 2001 Soil and water quality considerations in shrimp farming In Methods for improving shrimp farming in Central America Managua Nicaragua Central American University Press

Pai SS Anas A Jayaprakash NS Priyaja P Sreelakshmi B Preetha R Philip R Mohandas A and Singh ISB 2009 Penaeus monodon larvae can be protected from ltigtVibrio harveyiltigt infection by pre-emptive treatment of a rearing system with antagonistic or non-antagonistic bacterial probiotics Aquaculture Research 9999(9999)

Hai NV Buller N and Fotedar R 2009 The use of customised probiotics in the cultivation

Wang et al (16) evaluated a consortium of commercial probiotics (Bacillus sp pho-tosynthetic bacteria yeast Saccharomyces cervisiae Nitrosomonas sp and Nitrobacter sp) and found that the feed conversion ratio and population density of beneficial bacterial flora was improved compared with control treatments They also found that

the use of probiotics significantly increased dissolved oxygen concentrations and signifi-cantly reduced dissolved phosphorus and total inorganic nitrogen concentrations as well as decreasing the chemical oxygen

This study highlights the use of probiot-ics to improve water quality which in turn will reduce stress on the prawns making them less susceptible to disease

ConclusionIntensification of prawn production

subsequent increases in disease and anti-biotic resistant strains of bacteria ampli-fies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the micro-bial community of farms is developing This article has clearly demonstrated numerous studies that show that probiotics can benefit prawn production It should also be stressed that the increased demand for

Intensification of prawn production subsequent increases in disease and antibiotic resistant strains of bacteria amplifies the need for alternatives to prevent disease outbreaks and the concept of maintaining rather than fighting the microbial community of farms is developing

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

F Probiotic

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

PRODUCT IMAGEW=80mm x H=50mm

COMPANY LOGOW=110mm x H=30mmCONTACT DETAILS

QUARTER PAGE ADVERTW=190mm x H=58mm

PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

8 page full colour document - 100 copies = UKpound489 (please add UKpound15 for every additional 100 copies)

Delivery charged on a case by case basis

For more details please visit wwwaquafeedcoukreprints

1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

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IAF1001

Buy a paper copy ofthis issue

(UKpound15 per copy)

Subscribe to IAFSubscribers to IAF receive 5 issues

per year plus a copy of theInternational Aquafeed Directory amp

Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

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Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

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Media Partners

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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If you have a product or service that you want to advertise but we do not have a heading let us know

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Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

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BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 25: March | April 10 - International Aquafeed

22 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 23

TECHNICALSPECIFICATIONS100 words

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PRODUCT INFORMATION200 words

Company Overview International Aquafeed possesses a passion for aquafeed and an excitement about the technology that will make aquaculture

production fit for the next decade We strive to be the most respected provider of information about aquafeed in the widest sense

of the wordFeed accounts for almost 70 percent of the cost of producing farmed fish As a result getting feeding formulations and strategies right

can have a resounding impact on the business of producing farmed fish

Therefore in each issue we look in-depth at a range of technical issues associated with aquafeed production and itrsquos use on farm

We bring our readers global news about technological and research developments feed ingredients and micro-ingredients advances

market trends and market indicators and all issues that impact the aquafeed supply chain

Product information - Reprints

We can produce either 4 or 8 page versions of any specifi c feature that has appeared in the magazine (including back issues up to

2006) These features have been used by our customers as point of sale materials promotional materials for shows and exhibitions etc

Features can have existing adverts replaced or removed and can have a customised cover placed onto the document

For full details please contact James Taylor on +44 1242 267706 or Email jamestaquafeedcouk

Basic Prices4 page full colour document - 100 copies = UKpound294 (please add UKpound10 for every additional 100 copies)

8 page full colour document - 100 copies = UKpound489 (please add UKpound15 for every additional 100 copies)

Delivery charged on a case by case basis

For more details please visit wwwaquafeedcoukreprints

1 | INTERNATIONAL AQUAFEED |

PROFILE Technology

International Aquafeed magazine

7 St Georgersquos Terrace St James Square

Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267706 Fax +44 1242 267701Email carolinewaquafeedcouk

Web wwwaquafeedcouk

Technical SpecificationInternational Aquafeed magazine feature article reprints

bull 4 - 8 Pages bull Exsisting advertising materials replaced with your own

bull Full issue coverbull 150sgm gloss paper bull Delivery to any international address

bull For use as Point of sale materials exhibition handouts etc

For more information please contact James Taylor

Tel +44 1242 267706Email jamestaquafeedcouk

Tech_Profile_IAFindd 1

23022010 1126

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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IAF1001

Buy a paper copy ofthis issue

(UKpound15 per copy)

Subscribe to IAFSubscribers to IAF receive 5 issues

per year plus a copy of theInternational Aquafeed Directory amp

Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

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Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

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I ND U S

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Media Partners

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THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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If you have a product or service that you want to advertise but we do not have a heading let us know

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MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

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Extru-Tech Inc - wwwextru-techinccom

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

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from raw material processing mixing and extruding to drying Process expertise

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The solution behind the solution

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fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 26: March | April 10 - International Aquafeed

MADE IN SCOTLAND

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

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Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

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________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

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International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

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(UKpound15 per copy)

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Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

You want to know more about our event Then please visit wwwsummit2010org

Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

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EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

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Y

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idl10 - Grain Feedai 1222010 45438 PM

40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

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Equipment for salebull A Foeth BVbull Condex (UK) Ltd

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Extrudersbull Almex

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Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 27: March | April 10 - International Aquafeed

Leading aquaculture equipment manufacturers Fusion Marine and Gael Force have teamed up to provide fully integrated fish pen systems These advanced systems include extra strong polyethylene fish pens from Fusion Marine complete with safety decking nets sinker tubes and bird net supports Fusion Marine pens are used throughout the world for salmon trout sea bream bass tuna and many other species and because

of the strength of the pens are being used increasingly in exposed offshore locations

The two types of barges produced by Gael Force are the Seacap and Seamate feed barges which range in capacity from 50t - 400t These are made from high density concrete which gives the barge a design life of 40 years Accommodation can range from a day office right up to interior designed living quarters for up to four people Like Fusion Marine Gael Force have manufactured their product to cope with the most exposed conditions Gael Force are now designing their next generation of open ocean barges with designs due for release shortly

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

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Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

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E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

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________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

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E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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(UKpound15 per copy)

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Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

You want to know more about our event Then please visit wwwsummit2010org

Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

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Y

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

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Y

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Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

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Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

  1. Button 1
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Page 28: March | April 10 - International Aquafeed

The process and ingre-dient flow through an extrusion system begins with the raw materials

being received stored cleaned ground mixed and conveyed before reaching the extruder

Assume that the prepared mix is homogenously processed up to this point and placed in the dry ingredient feed bin for an extruder At this point small levels of ingredient changes have occurred already and would generally be associated with the grinding step and are considered to be strictly related to mechanical damage to the ingredients such as fractured starch cells

The extrusion process begins with the metering of the dry formula into a preconditioner which is a device designed to preheat moisten and mix before the material enters the extruder barrel

Additional ingredients usually in liquid form can also be added into the preconditioning device and is slaved to the dry feed rate as a percentage

Not all preconditioners perform equally and preconditioner designs vary greatly

In general a properly engineered conditioning cylinder will have the volume and design to evenly hydrate and heat the mix over a time period to achieve the desired effect in this unit operation

What happens to the ingredients in this area of the process

Predominately as long as the particle size is relatively even all of the formula particles absorb the moisture normally injected in the cylinder

The amount of water added is related to the style of aquatic feed being processed as

Protein damagecomplexation during extrusion

by Joseph P Kearns Wenger Manufacturing inc Sabetha Kansas uSA Email jkearnswengercom

Figure 1

Extruded aquatic feeds are a combination of selected ingredients based on their nutrient composition which normally include proteins from animal and plant sources carbohydrates oils vitamins and minerals Discussions will include what happens to the major ingredients or proteins and carbohydrates when passed through an extrusion cooker as both are interrelated

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

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FEATurES ArCHiVE

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32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

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-------------------------------------------------------------------------------------------

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________________________________________________________

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From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

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International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

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Tuti TanSubscription ManagerInternational Aquafeed magazine

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Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

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The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

bull Crevetecbull Danisco Animal Nutritionbull Exquimbull Ge-Pro Aquabull Inve Nutri-Ad

Elevator buckets

Equipment for salebull A Foeth BVbull Condex (UK) Ltd

bull Filter Screen Supply Ltd

Extrudersbull Almex

bull Anderson Internationalbull Baker Perkinsbull Brabender

bull Caliber Controlbull Conforma Clad Incbull Coperionbull ESE Intecbull Fudex Group Spabull Insta Pro International

bull Setrembull Suehiro EPMbull TGC Extrusionbull Wenger

Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 29: March | April 10 - International Aquafeed

well as the ingredients used As examples when making a floating feed that is lower in protein the amount of water added is less then when a high protein sinking feed is produced

Steam is also added to heat and do a degree of precooking of the mix prior to the extruder barrel Added water increases the rate of heat transfer in the mix making the added steam increase the temperature of the mix more efficiently

At this point in the process the ingredi-ents are typically elevated in temperature to somewhere near 90 to 95 degrees C The moisture levels are elevated to approx-imately 18 to 32 percent The starch and protein cells are hydrated with the starch cells being gelatinized to a level somewhere between 15 to 70 percent cook

Approximately 15 percent cook levels are seen in older single cylinder designs or cylinders not engineered for elevat-ing the gelatinization level generally due to low retention times As a note care should be taken to understand the differ-ence in gelatinisation level tests that can be preformed in labs as the Differential Scanning Colorimetry test method gives

higher cook values than the Enzymatic G e l a t i n i s a t i o n test method used by most cereal chemists and the reported cook level difference can be as much as 30 percent

A d v a n c e d precondit ioners are now available to achieve in the 70 to 80 percent gelatinisation level of the starches prior to the extru-sion barrel

At this point it maybe asked why discuss starch when this paper is about changes to the proteins during extrusion

Generally warm water fish can han-

Protein damagecomplexation during extrusion

26 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 27

F Extrusion

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

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International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

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DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 30: March | April 10 - International Aquafeed

As an example when reviewing the soy protein level in a feed if none of the protein is from soy the SME would be around 28kW-hrtonne If the level of soy protein increases to 100 percent of the total protein then the SME required nearly doubles to the 60kW-hr tonne

The protein and starch melt curves (Figure 2) indicate the relationship of tem-perature and water for various ingredients This chart shows that it is easier to achieve a melt transition (flowable state) with proteins as opposed to the starch fraction which in this case is shown as rice It can be assumed that if you focus on the gelatinisation of the starch the proteins will be denatured or cooked

This chart was developed using a Phase

The extruder barrel is where the final cooking occurs as well as defining the end product characteristics There are many possibilities in this area to consider regarding the control of the final product specifications and are beyond the scope of the discussion in this paper

All relate to both thermal or mechani-cal energy inputs and their effect on the final product As mentioned above the need for higher SME inputs for formulations high in vegetable proteins can be accomplished by die open area adjustment use of mid barrel or back pressure valves configurations changes in the barrel as well as extruder shaft speed control all of which can impart mechani-cal energy into the feed

dle the proteins which are usually lower levels and mainly from vegetable sources when compared to cold water fish feeds that usually have higher levels of marine protein

In either case the starch is added to hold the other ingredients together to provide the required water stability

Having mentioned different protein sources it should be noted that they do process differently

Normally animal and marine proteins require less water and less energy inputs during extrusion then vegetable based pro-teins Typically 15 percent vegetable protein requires about 20 percent moisture dur-ing extrusion while 30 percent vegetable protein needs in the area of 26 percent moisture or about a 25 percent increase in required water to make a quality feed

This statement ties in with precondition-ing being important followed by enough potential specific mechanical energy (SME) input in the extruder barrel to make a full range of possible formulations Figure 3 Light Microscopy

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

F Extrusion

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

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Quick Links

Contact the teamMore About Us

IAF1001

Buy a paper copy ofthis issue

(UKpound15 per copy)

Subscribe to IAFSubscribers to IAF receive 5 issues

per year plus a copy of theInternational Aquafeed Directory amp

Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

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Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

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I ND U S

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Media Partners

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THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

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BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 31: March | April 10 - International Aquafeed

28 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 29

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

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Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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IAF1001

Buy a paper copy ofthis issue

(UKpound15 per copy)

Subscribe to IAFSubscribers to IAF receive 5 issues

per year plus a copy of theInternational Aquafeed Directory amp

Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

You want to know more about our event Then please visit wwwsummit2010org

Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

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MY

CY

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

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Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

bull Crevetecbull Danisco Animal Nutritionbull Exquimbull Ge-Pro Aquabull Inve Nutri-Ad

Elevator buckets

Equipment for salebull A Foeth BVbull Condex (UK) Ltd

bull Filter Screen Supply Ltd

Extrudersbull Almex

bull Anderson Internationalbull Baker Perkinsbull Brabender

bull Caliber Controlbull Conforma Clad Incbull Coperionbull ESE Intecbull Fudex Group Spabull Insta Pro International

bull Setrembull Suehiro EPMbull TGC Extrusionbull Wenger

Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

  1. Button 1
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Page 32: March | April 10 - International Aquafeed

or other desirable chemical and physical modifications

Figure 3 shows microphotographs of starch and proteins in a feed

Sample 1 shows intact starch cells Sample 2 and 3 show samples with a

degree of cook and how the protein matrix holds the starch cells together Sample 4 is a high shear cook and you can see the protein and starch cells have been totally destroyed and blendedmelted together in an even matrix

Many studies have indicated that protein

effect does occur in aquatic feeds but at a lower level as normally we are not extrud-ing 100 percent proteins It can be done as covered in US Patent 4981711 Textured Sinking Food for Marine Life where no starch was added in the mix and protein sources only were used

Cross linking did occur and resulted in a meat like product designed to be similar to the natural foods eaten by shrimp and other bottom dwellers

Irreversible bonded dispersion Extrusion provides a homogeneous

i r r e v e r s i b l e bonded disper-sion of all micro i n g r e d i e n t s throughout a prote in s tarch matrix This not only insures uniformity of all ingredients put also provides a means whereby minor ingre-dients can be intimately associ-ated with poten-tial reaction sites promoting cross linking

Transition Analyzerreg which models the extrusion process accurately

Harper reported in 1986 that extrusion is widely used to accomplish the restructur-ing of protein-based foodstuffs for use in a wide variety of convenience foods contain-ing textured vegetable proteins

When mechanical and thermal energy are applied during the extrusion process

the macromolecules in the proteinaceous ingredients lose their native organized structure and form a continuous visco-elastic mass The extruder barrel screws and die align the molecules in the direction of flow

This realignment lsquoexposes bonding sites that lead to cross-linking and a reformed expandable structurersquo that creates a chewy texture in fabricated foods (1) This same

Figure 2

The different effects from a

variety of processing methods

will and do have an effect on

the feed qualities in water as

well as their performance

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

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From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

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International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

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7th - 8th October I The 10th GLOBALGAP Conference I London UK

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

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Directorate General of Livestock ServicesDepartment of Agriculture

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

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Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

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IAN

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DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

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Attractants

bull Crevetecbull Danisco Animal Nutritionbull Exquimbull Ge-Pro Aquabull Inve Nutri-Ad

Elevator buckets

Equipment for salebull A Foeth BVbull Condex (UK) Ltd

bull Filter Screen Supply Ltd

Extrudersbull Almex

bull Anderson Internationalbull Baker Perkinsbull Brabender

bull Caliber Controlbull Conforma Clad Incbull Coperionbull ESE Intecbull Fudex Group Spabull Insta Pro International

bull Setrembull Suehiro EPMbull TGC Extrusionbull Wenger

Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 33: March | April 10 - International Aquafeed

complete even matrix desired for maximum water durability

The different effects from a variety of processing methods will and do have an effect on the feed qualities in water as well as their performance

complexes formed during extrusion do not hinder digestion in the gut due to acidic envi-ronments which breakdown the complex

The extrusion process is one that can be used to impart a wide variety of energy lev-els both thermal and mechanical into a feed mix This variety of processing potential allows for engineering of the final feedstuff

Floating sinking slow sinking as well as the length of water stability and other variables can be controlled Is it desirable to have some intact protein and starch cells which allow for swelling in water or is a

and starch can complex with lipids in the recipe during the extrusion process As much as 30 percent of the lipids present have been shown to form complex compounds which cause the lipid fractions involved in the complexes to not be extractable during the ether extraction step (Soxhlet method of crude fat determination)

It is necessary to use an acid hydrolysis treatment as a preliminary step to free lipids from this complex prior to ether extraction to determine crude fat values

Studies have also indicated that these

fo r Mo re in fo rMatio n

Wenger Manufacturing IncTel +1 7852 842133Website wwwwengercom

30 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 31

F Extrusion

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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IAF - Links

Quick Links

Contact the teamMore About Us

IAF1001

Buy a paper copy ofthis issue

(UKpound15 per copy)

Subscribe to IAFSubscribers to IAF receive 5 issues

per year plus a copy of theInternational Aquafeed Directory amp

Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

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Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

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Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

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I ND U S

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Media Partners

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LIVESTOCK AND FEED INDUSTRY EVENT

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

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BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 34: March | April 10 - International Aquafeed

The low energy input of vertical units assures that mixing occurs without shear

Most importantly the concept of mixing using a vertical transport screw allows all products to be exposed over time at the top of the mixing vessel

This product exposure provides the ideal medium for the uniform applica-tion of liquids or powders The estab-lishment of a vacuum on the inherently strong conical mixing vessel results is a totally new concept in Vacuum Coating

Andritz Feed amp Biofuel provides a new tool for extruded product manufacturers a tool that

bull Affords simultaneous cost reduction and quality enhancement

bull Potentially opens the door to the production of wholly new categories of products in the future

The aqua feed industry has used vacuum coating for several years but have had prob-lems maintaining performance levels with conventional coaters

The future will require high-energy products based on protein sources hav-ing poor liquid absorption properties This coupled with micro liquids addition places stringent demands on tomorrowsrsquo vacuum coating systems

The petfood industry currently utilizes a wide variety of externally applied liquids and powders Drum coating systems fulfil

the normal requirements however with vacuum coating several benefits not pos-sible with atmospheric coating can be achieved

A vacuum batch coating system especially designed for aquafeed and pet food

For years Vertical mixers provided gentle mixing of feed ingredients Now Andritz Feed amp Biofuel adapts vertical mixing to vacuum coating technology

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

F Vacuum

fo r Mo re in fo rMatio n

Andritz Feed amp BiofuelGlentevej 5-7Esbjerg DK ndash 6705 DenmarkTel +45 7216 0300Fax +45 7216 0301Email andritz-fbandritzcomWeb wwwandritzcom

book store wwwperendalecombooks

Are you a Perendale bookwormPerendale Publishers Ltd the publishers of International Aquafeed has set up an online Amazon-based lsquoBook Shoprsquo that lets you browse a wide range of recently-published reports and books on aquaculture From early 2010 you will be able to read an extended review before making your selection and purchasing directly from AmazonConsult Perendale Publishers Online Book Store at wwwperendalecombooks

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

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Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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(UKpound15 per copy)

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per year plus a copy of theInternational Aquafeed Directory amp

Buyers Guide

International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

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If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

You want to know more about our event Then please visit wwwsummit2010org

Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

bull Crevetecbull Danisco Animal Nutritionbull Exquimbull Ge-Pro Aquabull Inve Nutri-Ad

Elevator buckets

Equipment for salebull A Foeth BVbull Condex (UK) Ltd

bull Filter Screen Supply Ltd

Extrudersbull Almex

bull Anderson Internationalbull Baker Perkinsbull Brabender

bull Caliber Controlbull Conforma Clad Incbull Coperionbull ESE Intecbull Fudex Group Spabull Insta Pro International

bull Setrembull Suehiro EPMbull TGC Extrusionbull Wenger

Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 35: March | April 10 - International Aquafeed

EMAiL NEWSLETTEr

Want more news about the aqua-culture feed industry then why ot sign up to the online newsletter Get updates about the magazine links to interesting arcticles addi-tional event information as well as industry updates The news letter is sent out every month and it is completely free of charge to sign up The newsletter also gives you a direct link to new features that are appearing in the features archive

FEATurES ArCHiVE

The International Aquafeed features archive is growing Find content from back issues of the magazine view individu-al features online or download a pdf version for your records

To view all of our currently live features please visit

wwwdocstoccomprofilePerendale

Or visit our full issue online archive at

wwwperendalecomarchivephp

32 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 33

Recomended websites

To advertise in the IAF Market Place please contact Caroline Wearn

Upcoming Events

21st - 23rd February 10GLOBALGAP WWF Aquaculture Dialogues

Booth F-53 Hall 5 Fish InternationalBremen Germany Contact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 KoelnGermany

T +49 221 57993 25 | F +49 221 57993 89

E meifertglobalgaporg | W wwwglobalgaporg

-------------------------------------------------------------------------------------------

GLOBALGAP will offer an expansion to its programme soaquaculture producers can be certified in one step for implementingpractices that help ensure the production of safe food in conjunctionwith environmentally and socially responsible farming throughcompliance of both GLOBALGAP Aquaculture Standards and thestandards created by the Aquaculture Dialogues World Wildlife Fund(WWF) coordinates the Dialogue processFor more information visit us Hall 5 at Stand F-53 -- at the 12thFishInternational Trade Fair in Bremen from 21-23 February 2010Visit httpwwwworldwildlifeorgaquadialogues for more information

1st - 5th March 10AQUACULTURE 2010

San Diego California USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E mariostaelscarletbe | W httpwwwmareventcom

3rd - 5th March 10 Victam Asia 2010

Queen Sirikit National Convention CenterBangkok Thailand Contact Andy West PO Box 411 Redhill RH1 6WE UK

T +44 1737 763501 | F + 44 1737 762996

E andrewwest733ntlworldcom | W httpwwwvictamcom

________________________________________________________

Buhler global partner for the feed production industry masters all theprocess operations required for transforming raw materials intovaluable animal feeds At the VictamGrapas Asia 2010 Buhlerpresents solutions for a safe and cost-effective production of high-quality feed and aqua feed The company delivers the completetechnology for cleaning weighing conveying grinding mixinghygienizing and drying of feed and aqua feed products Buhler continuously develops the Asian market and operates its ownbrand new manufacturing sites in China and India in addition toestablished branches in several Asian countries Buhler - booth 601 701

20th - 23rd April 2010 III Global Feed amp FoodCongress

Cancun MexicoContact Mr Roger Gilbert7 St Georges Terrace St James SquareCheltenham GL50 3PT UK

T +44 1242 267702 | F +44 1242 267701

E rogergilbertififorg | W httpwwwglobalfeed-foodcom

16th - 19th May 10 Alltechs 26thInternational Animal Health amp NutritionSymposium

Lexington Convention Center LexingtonKentucky USA Contact Lauren Ashley Pope 3031 Catnip Hill Pike USA

T +1-859-881-2213 | F +1-859-887-3256

E symposiumalltechcom | W httpwwwalltechcom

19th - 20th May 10 AQUACULTURE UK 2010

Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street TainRoss amp Cromarty IV19 1BH Scotland

T +44 1862 892188

F infoaquacultureukcom | W httpwwwaquacultureukcom

23rd - 26th May 10 Australasian Aquaculture 2010

International Conference and Trade ShowHotel Grand Chancellor Hobart TasmaniaAustralia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317Australia

T +61 437 152 234 | F +61 2 4919 1044

E sarah-janedayaquacultureorgau

W httpwwwaustralian-aquacultureportalcom

16th - 18th June 10Offshore Mariculture Conference 2010

Hilton Imperial Hotel Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill LowerQuay Fareham Hants PO16 0RA UK

T +44 1622 820622

E irobertsmercatormediacom | W wwwoffshoremariculturecom

5th - 9th July 10 The 9th InternationalCongress of the Biology of Fish

Hotel Sehrs Campus in the UABCampus at Bellaterra - Barcelona(Spain) Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037Barcelona Spain

T +34 93 2212955 | F +34 93 4592059

E garciamondial-congresscom

W httpsidcienciesuabes9FishBiologyCongress

8th - 10th July 10Indo Livestock 2010

Jakarta Convention Center Indonesia Contact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

T +62 21 8644 756 | F +62 21 8650963

E devinapindocom | W wwwindoaquaculturecom

6th - 8th October 10Aquaculture Europe 2010

Porto Portugal Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 GentBelgium

T +32 9 2334912 | F +32 9 2334912

E ae2010aquaculturecc | W httpwwwmareventcom

7th - 8th October 10GLOBALGAP Summit 2010

HILTON London Metropole Hotel London UK Contact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

T +49 2215 7993693 | F +49 2215 799389

E kretschmerglobalgaporg | W httpwwwsummit2010org

International Aquafeed magazine - 7St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom Tel +44 1242 267706 | Fax +44 1242 267701 | Email nickybaquafeedcouk | Web wwwaquafeedcouk

From Tuti Tan lttutitaquafeedcoukgtSubject International Aquafeed newsletter 0210

Date 10 February 2010 212628 GMT

To jamestgfmtcoukReply-To tutitaquafeedcouk

Having trouble viewing this email Click here

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IAF1001

Buy a paper copy ofthis issue

(UKpound15 per copy)

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International Aquafeed magazine

Newsletter - February 2010

Dear Subscriber

Welcome to the second IAF Newsletter of 2010

We have a number of important events listed below and what will bein the up-coming issue

To find out what is in our current issue please click here

If you would like to receive a no-obligation trial issue of our printedversion please contact me

Sincerely

Tuti TanSubscription ManagerInternational Aquafeed magazine

Follow IAF on

If you do not wish to receive subsequent copies of the Newsletter please use the unsubscribe linkat the foot of this page

For advertising opportunities in IAF please contact Caroline Wearn

Industry News

A bold step for BIOMIN aquacultureSponsorship of a keynote session at Aquaculture 2010 confirmsBIOMINs firm commitment and keen focus on this dynamic growthsector BIOMIN embraces the new year with a bold plunge inaquaculture For the first time BIOMIN will be partnering the WorldAquaculture Society (WAS) with the sponsorship of the Aquafeedssession at Aquaculture 2010Read more

________________________________________________________

News from the portal Below is a selection of news stories from the International AquafeedNews portal The portal is a constantly updating source of news fromaround the world To visit the portal page please click here

Chile will launch its first deepwater salmon farming projectSeen on wwwupicom

Chile will launch its first deepwater salmon farming project with aNorwegian loan to try and reverse the low yields on salmon amidconstant global demand Read more

Alaska pollack receives international approvalSeen on wwwfishupdatecom

ALASKA Pollack which is being increasingly used by British seafoodproducers as an alternative to cod and haddock has been deemedsustainable under the criteria developed by the Marine Stewardshipby the international certification body Moody Marine and a team ofthree independent expertsRead more

Economy of scalesSeen on wwwscmpcom

A fish farm in the unlikely setting of Chai Wan is bringing cheaper andmore eco-friendly seafood to Hong Kong tables Anyone ready toforego a tasty grouper to protect rapidly dwindling fish stocks Formost restaurants and foodies in Hong Kong the response would beeat first and let someone else worry about the consequencesRead more

In our next issue

Protein sources - Alternative protein sources should alsoshow functionality if they are to play a role in modernaquafeedsThe aquaculture industry today faces a number of challengesthat threaten to restrict its development and profitability

Evaluation of probiotic bacteria in prawn productionGlobally two million metric tonnes of prawn valued at US$144billion were produced in 2004(1) with large industry growthobserved in Asian countries

Protein damagecomplexation during extrusionExtruded aquatic feeds are a combination of selectedingredients based on their nutrient composition which normallyinclude proteins from animal and plant sources carbohydratesoils vitamins and minerals Discussions will include whathappens to the major ingredients or proteins and carbohydrateswhen passed through an extrusion cooker as both areinterrelated

A vacuum batch coating system - especially designed foraqua feed and pet foodFor years Vertical mixers provided gentle mixing of feedingredients Now Andritz Feed amp Biofuel adapts vertical mixingto vacuum coating technology

Aquafeeds - ready to take the plungeAlternative - a buzzword in popular culture - aptly describesthe current situation with regards to ingredients in aquafeedproduction Dwindling supplies of fishmeal present one of theforemost challenges for the global aquaculture industry todayand the search is on for new sources that can replacefishmeals nutritional quality without compromising on animalperformance

Feed Management - Aquafeed in TanzaniaAquaculture in Tanzania is still minimal and is practiced in bothfreshwater and marine environments Freshwater species aretilapia (Nile tilapia Oreochromis niloticus Mozambique tilapiaO mossambicus and Zanzibar tilapia Tilapia hornorum) whichare cultured by small scale fish farmers and rainbow troutOncorhynchus mykiss which is cultured commercially

Feed Management - Diets for tilapia brood stockAddressing challenges for reducing dependency on fish oilThe Nile tilapia Oreochromis niloticus is a popular species forfarming species because it grows and reproduces well under awide range of environmental conditions and productionsystems

Highlights in the current issue Below are the main features from the January issue of InternationalAquafeed To view the features using our Features Archive onwwwdocstoccom please use the links below

To view the magazine in full please click here to go to the onlineversion

Evaluation of Fishmeal Substitution

Gilthead sea bream production inMediterranean countries increasedfrom 30000 tons in 1996 to 90000tons in 2005which mean that saleprices dropped considerably from66eurokg in 1996 to 5eurokg in 2005with an historic minimum of 4eurokgin Read more on docstoc

Preventing the impact of seawater transfer on feedintake in salmon

Changes in diet environment orphysiology represent many stressfactors for farmed animals that canimpact their appetite health andperformance In salmon farmingthe transfer of smolts fromfreshwater to seawater is astressful event Read more on docstoc

Active ingredients for healthy animals Rice - thetechnological ingredient for aquafeed

With a production of over 600million tonnes per year rice is themost important food-cereal andserves as a basic ingredient forpeople all over the world On theother hand only six percent of thetotal rice production is used inanimal nutrition Read more on docstoc

To advertise against upcoming features IAF please contact Caroline Wearn

Global Market Place Are you looking for products and services in the AquacultureindustryThe International Aquafeed Market Place is now online and growingIn the market place you will find information about various companiesin the aquaculture industry and their related products and service thatwill be of interest to your business

The view the Market Place click here

If you would like to have your company involved in the Market Placeplease contact Caroline WearnTel +44 1242 267706Emailcarolinewaquafeedcouk

Tuti Tan

Not In Address Book

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

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Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

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Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

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EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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If you have a product or service that you want to advertise but we do not have a heading let us know

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

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Page 36: March | April 10 - International Aquafeed

Alternative ndash a buzzword in popular culture - aptly describes the current situation with regards to

ingredients in aquafeed production Dwindling supplies of fishmeal present one of the foremost challenges for the global aquaculture industry today and the search is on for new sources that can replace fishmealrsquos nutritional quality without compromising on animal performance

Addressing the topic of sustainable solutions for aquafeed the Asian aquafeed conference held in conjunction with the Livestock Asia expo in Kuala Lumpur last October presented insights into optimising aquafeeds balancing profit and performance in aquaculture and new strategies and alternative ingredients for the industry

Growth marketsHigher incomes in the developing world

a perception of aquaculture products as premium and dietary changes have fuelled the boom in aquaculture production The availability of fishmeal and other protein sources has also ignited the growth of the relatively young industry in the animal pro-tein sector Aquaculturersquos share of fishmeal consumption has increased over the years vis-agrave-vis terrestrial animal species

Accounting for the bulk of the global increase in demand for aquafeeds is Asia

This is driven largely by China and the huge potential in its 20 million tonne fresh-water fish segment India another rapidly

growing aquaculture market is also witness-ing a significant rise in aquafeed demand as producers shift from single component farm-based feeds to formulated and commercial feeds and species diversification

In Southeast Asia a wide variety of local-ly available protein sources such as copra soy fishmeal and canola has supported the regionrsquos aquaculture industry From 2000 to 2007 while world aquaculture produc-tion rose 27 percent and Asian production increased 64 percent Southeast Asia saw growth of 143 percent during that period

Out of 25 million tonnes of aquafeed produced in 2008 it was estimated that four million tonnes came from Southeast Asia At current rates of aquaculture growth the potential for aquafeed is vast

In Latin America the proximity to the North American market and increasing domestic demand are fueling the aquacul-ture industry particularly for salmonids

Turkey and Egypt are strong players in the marine fish and tilapia sectors respec-tively with young and growing populations

While options for alternative raw materi-als are not readily available in North America and Europe new technologies that help to increase feed utilisation and reduce anti-nutritional factors will continue to shape the development of the global aquafeed business

Also the opening of lsquonew marketsrsquo in the West - countries where aquatic products are not featured in the traditional diet ndash and the receptiveness of lsquonew speciesrsquo such as tilapia in Europe and North America are new areas of development for aquaculture producing countries

Protein alternativesAs aquaculture continues to grow the

replacement of fishmeal with alternative protein sources is becoming a pivotal trend for the global industry Volatile supply and escalating costs of production for fishmeal and fish oil and falling prices for some aquatic products like P vannamei shrimp are pushing the search for new ingredients in aquafeed

Sustainable fish production also dictates that no more than one kg of fish is used to produce one kg of fish The sustainable development of the aquaculture industry would hinge increasingly on the ability to utilise protein replacement sources effec-tively in terms of costs availability and species performance

As the industry weans itself off fishmeal usage of soybean meal in aquaculture has risen steadily

The rise in seafood consumption and overall growth in meat demand paints a bullish picture for soybeans with increased production in grower countries and greater applications of the meal in animal and aquatic feed

Although current proportions in aquafeed are still smaller compared to feed for ter-restrial animals it is estimated that vegetable meals and concentrates will constitute about 75 percent of protein requirements in aqua-feeds by 2015 It is imperative in aquafeed to use only high quality well processed soybean or other vegetable meal that is consistent with a good amino acid profile

However replacing fishmeal with alter-natives is a tricky task

by Daphne Tan Communicating Agriculture

Aquafeedsready to take the plunge

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

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Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

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EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

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Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

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Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

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Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

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Page 37: March | April 10 - International Aquafeed

Fishmeal is a good source of amino acids and contains highly unsaturated lipid and fatty acids (EPA DHA) nutrients important to the growth of all fish species

In comparison vegetable protein sources have more than 15 different anti-nutritional effects and when used in substitution affect the palatability of aquaculture diets Fishmeal is still preferred by producers as it is con-sidered the most natural and best feed raw material While land animal proteins and by-products offer good nutritional value for farmed fish market acceptance remains a challenge

But in the long term experts believe that perceptions will change in the face of science-based research and solutions For example the salmon industry had achieved protein retention levels of 45 percent and above by the year 2000 compared with 22-25 percent in the 1980s setting a positive precedence for new possibilities in the ingredient composi-tion of aquafeed for all species

Less protein better performance

Feeding fish is not simply about a spe-cific ingredient like fishmeal but a full pack of nutrients that addresses the speciesrsquo growth needs The current trend of for-mulating diets based on digestible amino acid levels rather than on a least cost basis allows nutritionists to tap on a wider range of ingredients and not depend on any one protein source

Applying the lsquoIdeal Protein Conceptrsquo ndash the level of protein when ingested by the animal that provides all necessary amino acids ndash leads to an overall reduction in crude protein intake without impairing growth and performance This also lowers the amount of nitrogen excreted in animal waste

The inclusion of energy sources helps improve protein utilisation in aquafeed

Enzymes that promote digestion or supplements such as probiotics that

enhance the utilisation of nutrients are also some trends in strategies to improve feed utilisation Palatability issues arising from fishmeal substitution can also affect feeding and growth and could be tackled with the use of pal-latants and attractants

Feeding strategies should also take into consideration the stage of growth of the species Nutrient requirements for each species and stage of growth should also be thoroughly understood so as to avoid over- or under-formulation At the same time reducing feed during the fry stage could result in higher FCR and increased fat deposit

With the development of feed formula-tion strategies based on nutri-ent availabilities costs are more closely linked to performance of the species rather than the cost of raw materials per se Costs can be measured in terms of gain in FCR or the composition of the fillet yield ndash meat gain as opposed to fat gain Improved feed extrusion t e c h n o l o g i e s are also paving the way forward for the aquafeed industry Rather

than shunning alternative ingredients the embrace of new technologies and feeding strategies would pave the way forward for the aquafeed industry

About the authorDaphne Tan is a Singapore-based free-lance journalist and analyst for feed and feed related issues She can be contacted on the details belowascribe ndash Communicating Agriculture Email ascribeaggmailcom

34 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 35

F Aquafeed

Table 1 Percentage of Production by Area (World Asia SEA crustaceans fishes [fresh marine diadromous]) 2000-2007

2000 2001 2002 2003 2004 2005 2006 2007

World vs Asia 25 21 18 17 12 9 6 3

Asia vs World 75 79 82 83 88 91 94 97

World vs SEA 91 90 90 88 86 85 84 82

SEA vs World 9 10 10 12 14 15 16 18

Asia vs SEA 88 87 87 86 84 83 83 82

SEA vs Asia 12 13 13 14 16 17 17 18

Source FAO Fisheries Statistics 2009

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

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Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

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Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

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I ND U S

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DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

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Page 38: March | April 10 - International Aquafeed

Aquaculture in Tanzania is still minimal and is prac-ticed in both freshwater and marine environments

Freshwater species are tilapia (Nile tilapia Oreochromis niloticus Mozambique tilapia O mossambicus and Zanzibar tilapia Tilapia hornorum) which are cultured by small scale fish farmers and rainbow trout Oncorhynchus mykiss which is cultured commercially

Marine species include seaweeds of the red algal species (Eucheuma denticula-tum and Kappaphycus alvarezii) (Bryceson 2002) prawns Penaeus monodon (Bryceson 2002) rabbitfish Siganus can-naliculatus (Bwathondi 1982) and milkfish Chanos chanos (Mwangamilo and Jiddawi

2003) clams ark clam Anadara antiquate and giant clam Tridacna maxima oysters pearl oysters Pinctada margaritifera and mangrove oysters Saccostrea cucullata and mud crab Scylla serrata (Rice et al 2006)

The bulk of fish production comes from tilapias which are kept mostly by subsist-ence farmers who regard fish farming as a secondary activity to provide protein to the household and little surplus for cash or barter

The fish are cultured in extensive to semindashintensive earthen ponds with sizes varying from 150ndash500m2 (Bjoneseth 1992) The ponds are usually fertilised with animal droppings or tender leaves as compost manure

Supplementary feeds commonly used are rice and maize bran kitchen leftovers and garden remains The use of formulated aquafeeds is almost non-existent

Fish are partially or totally harvested after six to 12 months with an average yield of 2089kg ha-1 year-1 (Wetengere et al 1998 Kaliba et al 2006) The yield is low compared to the 10000kg ha-1 year-1 which can be achieved if properly formu-lated supplementary aquafeeds were used (Jauncey 1998)

There are several potential feedstuffs that

can be incorporated in fish diets as shown in the table below

A few studies have been conducted to a evaluate suitability of some of the locally available ingredients in tilapia diets

Cotton seed cake and soybean meal were shown to be suitable as a main source of protein sources (Shoko 2002) Pigeon peas processed by either sprouting or roasting could replace up 40 percent of fish-meal without causing a significant reduction in growth performance (Mnembuka 2003)

Moringa and cassava leaf meals could pro-vide less than 15 percent of dietary protein while their composite could provide up to 20 percent (Madalla 2009) Cassava root meal could replace up 75 percent of the wheat meal without significantly reducing performance (Madalla 2009)

Adoption of these findings by farmers has been poor mostly due to the subsist-ence nature of fish farming which does not encourage investment in formulated feeds

Feed formulation has a cost implica-tion in terms of ingredients as well as the feed formulation knowledge Even if such knowledge is availed it will still be difficult to put it into practice due to the relatively high cost of ingredients relative to the returns due to the subsistence nature of aquaculture

ReferencesBjoneseth R (1992) A survey of freshwater fish farming in Tanzania Unpublished MSc Thesis Agricultural University of Norway

Bryceson I (2002) Coastal aquaculture developments in Tanzania sustainable and non-sustainable experience Western Indian Ocean Journal of Marine Science 1 (1)1-10

Bwathondi POJ (1982) Preliminary investigations

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

Green foragesGliricidia NA 117 200 38 167

Leucaena 926 94 246 34 171

Sesbania NA 93 221 33 173

Cassava 830 17 217 47 119

Mulbery Morus alba 890 145 196 22 129

Cereal grains amp by-productsMaize meal 899 155 106 35 25

Maize bran 895 28 105 106 71

Maize hominy 905 52 136 127 76

Rice polishing 918 137 110 52 113

Rice bran 911 158 93 43 279

Wheat bran 899 543 186 25 105

Wheat pollard 894 326 179 41 57

NA not available | Source Laswai et al (2002)

TanzaniaAquafeeds in

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

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stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

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Name

Jobtitle

Company

Address

Tel Fax

Mobile

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inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

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I ND U S

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PAR T E

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EN

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Media Partners

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THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

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To place a classified advert in our next issue and take

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42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

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In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 39: March | April 10 - International Aquafeed

on rabbitfish Siganus canalicullatus cultivation in Tanzania Aquaculture 27(3)205-210

Jauncey K (1998) Tilapia feeds and feeding Scotland Pisces Press Limited

Kaliba AR Osewe KO Senkondo EM Mnembuka BV and Quagrainie KK (2006) Economic Analysis of Nile Tilapia (Oreochromis niloticus) Production in Tanzania Journal of the World Aquaculture Society 37 (4)464-473

Laswai GH Mutayoba S K Temu A A and Kusolwa P M (2002) Feed Table Chemical composition of poultry feedstuffs in Tanzania Sokoine University of Agriculture and ASARECAFOODNET project

Madalla N (2009) Novel feed ingredients for Nile tilapia (Oreochromis niloticus L) Unpublished PhD Thesis University of Stirling United Kingdom

Mnembuka B V (2003) Studies on the use of pigeon peas (Cajanus cajan) as a protein supplement for Nile tilapia Unpublished PhD Thesis University of Dar es salaam Tanzania

Mwangamilo JJ and Jiddawi NS (2003) Nutritional studies and development of a practical feed for milkfish (Chanos chanos) culture in Zanzibar Tanzania Western Indian Ocean Journal of Marine Science 2 (2)137-146

Rice MA Mmochi AJ Zuberi L and Savoie RM (2006) Aquaculture in Tanzania World Aquaculture 37 (4)50-57

Shoko A P (2002) The effects of different diets and stocking density on the growth performance of Oreochromis variabilis (Boulenger 1906) under aquaculture conditions Unpublished Msc Thesis University of Dar es salaam Tanzania

Wetengere K Osewe K and Herwaarden H (1998) Development of semi-intensive fish farming in Morogoro region Tanzania ALCOM working paper no 22 FAOSIDA

table feed ingredients locally available in tanzania

Feed resource Biochemical composition in DM ()

DM Ash CP Cl CF

oil Seed Cake and legumesCotton seed meal (Undecorticated) 926 132 381 74 203

Cotton seed meal (Decorticated) 908 56 338 70 152

Sunflower seed meal (Decorticated) 944 55 329 177 327

Sunflower seed meal (Undecorticated) 943 49 258 123 432

Copra cake 869 61 223 84 120

Soybean (Boiled) 955 62 415 99 59

Soybean (Toasted) 889 70 487 31 72

Pigeon peas 926 45 209 18 10

Animal ProductsSardine ldquodagaardquo

fishmeal 924 2209 606 415 25

Nile Perch Body Frame Meal 935 367 415 77 105

Fish Waste 906 336 425 NA 86

Blood meals 973 91 851 09 NA

Bone meals 913 691 269 NA 217

Tubers

Cassava 874 57 28 25 36

NA not available | Source Laswai et al (2002)

36 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 37

Feed Management

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

You want to know more about our event Then please visit wwwsummit2010org

Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

bull Crevetecbull Danisco Animal Nutritionbull Exquimbull Ge-Pro Aquabull Inve Nutri-Ad

Elevator buckets

Equipment for salebull A Foeth BVbull Condex (UK) Ltd

bull Filter Screen Supply Ltd

Extrudersbull Almex

bull Anderson Internationalbull Baker Perkinsbull Brabender

bull Caliber Controlbull Conforma Clad Incbull Coperionbull ESE Intecbull Fudex Group Spabull Insta Pro International

bull Setrembull Suehiro EPMbull TGC Extrusionbull Wenger

Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

  1. Button 1
  2. Button 3
  3. Button 2
  4. Button 4
  5. Button 6
  6. Button 5
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  8. Button 8
  9. Button 9
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  17. Button 52
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Page 40: March | April 10 - International Aquafeed

The Nile tilapia Oreochromis niloticus is a popular species for farming because it grows and reproduces

well under a wide range of environ-mental conditions and production systems

So much so that tilapias are now the second most popularly farmed fish after carps in the world cultured in about 100 countries in tropical and subtropical regions According to FAO global tilapia production reached 24 million tonnes in 2006 around five percent of total aquaculture production

The sustainability of this production however will have to be underpinned by regular supply of quality seed which in turn will depend of high quality of brood stock diets for their production

One crucial aspect that will arguably question the sustainability of tilapia farming is the dependency on fish oil (FO) including brood stock diets

Moreover the availability and costs of fish feed ingredients such as FO are vital

factors for sustainable aquaculture par-ticularly if tilapia is to compete with other species It is therefore timely to explore substitutes for ingredients like FO to secure the future of tilapia farming by reducing dependence on marine pelagic fish for oil

Can we substitute for fish oil

One key require-ment of tilapia seed production is a high proportion of fertilised eggs that result in larvae with acceptable survival and growth It is widely recognised that brood stock nutrition affects reproductive performance and egg and larval quality in fish and there is now a sub-stantial body of literature highlighting the immense importance of lipids and essential fatty acids (EFA) egg and larval quality and

performance Much of this recent attention however has been on marine species

One potential replacement of FO for tilapia brood stock diets could be palm oil (PO) which is currently the worldrsquos

most important oil crop accounting for 25 percent of total vegetable oil production

Many scientific papers have shown that a significant part of fish oil can be sub-

Diets for tilapia brood stock

Table 1 Proximate composition of experimental diets (composition of diet expr

Proximate analysis

Diet 1 (Po)

Diet 2 (PampCo)

Diet 3 (control)

Dry mater 143 142 80

Crude protein 4101 408 406

Crude lipid 98 97 71

Carbohydrate 222 231 243

Ash 53 51 100

Crude fibre 73 73 78

Gross energy (KJg-1) 204 203 239

Addressing challenges for reducing dependency on fish oil

by Krishen rana and Ali Hajizadeh

38 | InternatIonal AquAFeed | March-april 2010

Feed Management

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

You want to know more about our event Then please visit wwwsummit2010org

Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

bull Crevetecbull Danisco Animal Nutritionbull Exquimbull Ge-Pro Aquabull Inve Nutri-Ad

Elevator buckets

Equipment for salebull A Foeth BVbull Condex (UK) Ltd

bull Filter Screen Supply Ltd

Extrudersbull Almex

bull Anderson Internationalbull Baker Perkinsbull Brabender

bull Caliber Controlbull Conforma Clad Incbull Coperionbull ESE Intecbull Fudex Group Spabull Insta Pro International

bull Setrembull Suehiro EPMbull TGC Extrusionbull Wenger

Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 41: March | April 10 - International Aquafeed

38 | InternatIonal AquAFeed | March-april 2010

HOT TOPICS

Good Agricultural Practice

7th - 8th October I The 10th GLOBALGAP Conference I London UK

SUMMIT2010

Farming for ConsumersEverything you need to know about Good Agricultural Practice certification in one place

Come and join us for the best net-working discussion and debates at the worldlsquos favourite conference event for Good Agricultural Practice Top International speakers from the private and public sector will provide key insights into Good Agricultural Practice developments around the globe Join our retailer and supplier members as they share with you how they are implementing GLOBALGAP into their global supply chains

7th - 8th October 2010HILTON London Metropole HotelLondon UK

You want to know more about our event Then please visit wwwsummit2010org

Learn about the New Version of the GLOBALGAP StandardResponsible Management of ResourcesResidue MonitoringSmallholder ProgrammesCertification IntegritySocial Practices in Primary ProductionAquaculture and Livestock TrendsLinkages to Consumer Labels

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

bull Crevetecbull Danisco Animal Nutritionbull Exquimbull Ge-Pro Aquabull Inve Nutri-Ad

Elevator buckets

Equipment for salebull A Foeth BVbull Condex (UK) Ltd

bull Filter Screen Supply Ltd

Extrudersbull Almex

bull Anderson Internationalbull Baker Perkinsbull Brabender

bull Caliber Controlbull Conforma Clad Incbull Coperionbull ESE Intecbull Fudex Group Spabull Insta Pro International

bull Setrembull Suehiro EPMbull TGC Extrusionbull Wenger

Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 42: March | April 10 - International Aquafeed

stituted with without negatively affecting fish growth feed utilization and survival but comparatively little is known if they be substituted in brood stock diets

Such a study was recently conducted at the Institute of Aquaculture to test the effect of palm oil on egg and larval quality over consecutive spawnings in brood stock Nile tilapia that were reared for their entire life cycle on their respec-tive diet regimes The three diets tested shown in Table 1 include 100 percent substitution of oil using PO (Diet 1) 90 percent PO (Diet 2) and a control trout diet (Diet 3)

Fish were fed three times daily at three percent of body weight Fish were manually stripped under anaesthesia and eggs were fertilised by adding the sperm from males maintained under the same diet regime and total fecundity (number of eggs per female) relative fecundity (no of eggs per female weight) fertilisa-tion () and hatching rate () inter-spawning-interval (ISI) and egg weight as percentage of body weight (EWBW) monitored In addition hatchlings from each individual fish were weighed and their length measured at 10 days post-fertilisation

Fecundity and egg sizeThese studies indicated that PO

had no negative effect egg diameter egg volume egg wet and dry weight and total egg volume ( although some differences were observed with relative fecundity with sub-stitute diets performing better Similarity the substitute diets did not negatively effect larval length and weight Table 2) although some differences were observed with relative fecundity with substitute diets performing better Similarity the substitute diets did not nega-tively effect larval length and weight

In each raw means with different superscripts are significantly differ-

ent (P lt 005) The inter spawning interval is an

important factor for understanding breeding performance In these studies ISI for brood stock fed substituted PO diets had the shortest ISI (Figure 1) an added benefit

These studies suggest that under con-trolled conditions palm oil performed as well with commercial control diets containing FO and could be used suc-cessfully for brood stock diets whilst also increasing the profitability of seed production

Table 2 Spawning performance of nile tilapia fed different dietary lipid sources

Parameters

treatments

Palm oil diet

(Diet 1)

PampCl oil diet (91)

(Diet 2)

Control

(Diet 3)

Total fecundity 7526plusmn3201b 8233plusmn4659a 6629plusmn3610b

Relative fecundity (no of egg BWg) 55plusmn023a 55plusmn038a 36plusmn031b

Egg diameter (mm) 22plusmn003a 22plusmn003a 22plusmn003a

Egg volume (mm3) 52plusmn022a 54plusmn022a 56plusmn024a

Total egg volume (mm3) 39027plusmn23645a 43857plusmn26711a 36546plusmn23707a

Egg dry weigh (mg) 26plusmn005a 25plusmn009a 27plusmn009a

Egg wet weight (mg) 61plusmn01a 61plusmn016a 66plusmn021a

EW BW 14plusmn006a 13plusmn008a 09plusmn008b

Fertilisation rate () 763plusmn140a 785plusmn182a 759plusmn22a

Hatchability () 595plusmn104a 601plusmn175a 614plusmn135a

ISI (day) 14plusmn071a 19plusmn152b 24plusmn274c

Under controlled conditions palm oil performed as well with commercial control diets containing FO and could be used successfully for brood stock diets whilst also increasing the profitability of seed production

40 | InternatIonal AquAFeed | March-april 2010

Feed Management

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

bull Crevetecbull Danisco Animal Nutritionbull Exquimbull Ge-Pro Aquabull Inve Nutri-Ad

Elevator buckets

Equipment for salebull A Foeth BVbull Condex (UK) Ltd

bull Filter Screen Supply Ltd

Extrudersbull Almex

bull Anderson Internationalbull Baker Perkinsbull Brabender

bull Caliber Controlbull Conforma Clad Incbull Coperionbull ESE Intecbull Fudex Group Spabull Insta Pro International

bull Setrembull Suehiro EPMbull TGC Extrusionbull Wenger

Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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  2. Button 3
  3. Button 2
  4. Button 4
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  49. Button 49
Page 43: March | April 10 - International Aquafeed

40 | InternatIonal AquAFeed | March-april 2010

INDONESIArsquoS NO1 LIVESTOCK AND FEED INDUSTRY SHOW

REPLY COUPON

Please send me more information on exhibiting at INDO LIVESTOCK 2010 EXPO amp FORUM

Please send me more information on visiting at INDO LIVESTOCK 2010 EXPO amp FORUM

For futher information please call fax toPT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 10 Billy amp Moon Pondok Kelapa Jakarta -13450 Indonesia Telp +6221 8644756 85 8650962 Fax +62218650963Email infoindolivestockcom

Name

Jobtitle

Company

Address

Tel Fax

Mobile

Email Gra

inF

eed

Directorate General of Livestock ServicesDepartment of Agriculture

Hosted by Organised by

Napindo

Supported by

PER

I ND U S

TR

IAN

DE

PAR T E

M

EN

DEPARTEMEN PERDAGANGANREPUBLIK INDONESIA

Media Partners

$ amp $ (

THE 5TH INDONESIArsquoS NO1

LIVESTOCK AND FEED INDUSTRY EVENT

85

BOOKED

wwwindolivestockcom

C

M

Y

CM

MY

CY

CMY

K

idl10 - Grain Feedai 1222010 45438 PM

Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

bull Crevetecbull Danisco Animal Nutritionbull Exquimbull Ge-Pro Aquabull Inve Nutri-Ad

Elevator buckets

Equipment for salebull A Foeth BVbull Condex (UK) Ltd

bull Filter Screen Supply Ltd

Extrudersbull Almex

bull Anderson Internationalbull Baker Perkinsbull Brabender

bull Caliber Controlbull Conforma Clad Incbull Coperionbull ESE Intecbull Fudex Group Spabull Insta Pro International

bull Setrembull Suehiro EPMbull TGC Extrusionbull Wenger

Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 44: March | April 10 - International Aquafeed

Classified Adverts

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Additivesbull Arcadian Seaplants Ltdbull Advanced Bionutritionbull Agresearch Inc

bull Kiotechagilbull Krill Canada Corpbull Inve Aquaculturebull Reed MAriculture Inc

Attractants

bull Crevetecbull Danisco Animal Nutritionbull Exquimbull Ge-Pro Aquabull Inve Nutri-Ad

Elevator buckets

Equipment for salebull A Foeth BVbull Condex (UK) Ltd

bull Filter Screen Supply Ltd

Extrudersbull Almex

bull Anderson Internationalbull Baker Perkinsbull Brabender

bull Caliber Controlbull Conforma Clad Incbull Coperionbull ESE Intecbull Fudex Group Spabull Insta Pro International

bull Setrembull Suehiro EPMbull TGC Extrusionbull Wenger

Maximum bulk storage

Pulverisersbull Aaron Equipmentbull Caterday Jacobsonbull Christy Turnerbull Fritsch GmbHbull Jaeckering

bull Stedmanbull Sterner Aqua Techbull Mill Technology

Services (publications)

Shrimp Feed additive

CLASSIFIED ADVERTISING

To place a classified advert in our next issue and take

advantage of our great extra online exposure please contact Caroline

WearnTel+44 1242 267706

Emailcarolinewaquafeedcouk

Advertise in-print amp online for the same price as a

print advert

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 45: March | April 10 - International Aquafeed

42 | InternatIonal AquAFeed | March-april 2010 March-april 2010 | InternatIonal AquAFeed | 43

Vacuumbull AampJ Mixingbull Andritz Feed amp Biofuel

bull GAME Engineering Ltdbull Stolz - Sequipag

Shrimp feed amp larval diet

bull Artemia International LLC

Supply chain

Add your own heading

If you have a product or service that you want to advertise but we do not have a heading let us know

and we can create one for you

MArKET PLACEThe extra online power of Classified Adverting

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 46: March | April 10 - International Aquafeed

AKVA group ASA - wwwakvagroupcom

Alltech Inc - wwwalltechcom

Amandus Kahl GmbH amp Co - wwwamandus-kahl-groupde

Andritz Feed amp Biofuel - wwwandritzcom

Biomin GmbH - wwwbiominnet

Buhler AG - wwwbuhlergroupcom

Dishman Netherlands BV - wwwdishman-netherlandscom

Extru-Tech Inc - wwwextru-techinccom

Forberg International AS - wwwforbergno

Geelen Counterflow - wwwgeelencounterflowcom

IFFO Limited - wwwiffonet

Jiangsu Muyang Group Co Ltd - wwwmuyangcom

Marevent - wwwmareventcom

Mercator Media Ltd - wwwoffshoremariculturecom

NORELampNATURE - wwwnorelynaturecom

nv SCE - wwwscebe

Ottevanger Milling Engineers BV - wwwottevangercom

Palm View Trade - wwwpalmviewtradecom

Perten Instruments AB - wwwpertencom

SPF (activite Aquativ) - wwwaquativ-dianacom

SpotMark - wwwsptmrkcom

University of Stirling - wwwaquastiracuk

Wenger Manufacturing Inc - wwwwengercom

Wynveen International BV - wwwwynveencom

In every issue of International Aquafeed we will be providing a list of companies and web links related to key stories amp topics within each specific issue

If you would like information on how your company can get involved please contact our Marketing Manager Caroline Wearn

Email carolinewaquafeedcouk |Tel +44 1242 267706

WEB LINKS

44 | InternatIonal AquAFeed | March-april 2010

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 47: March | April 10 - International Aquafeed

44 | InternatIonal AquAFeed | March-april 2010

The European Aquaculture Society is pleased

to welcome you to its AE2010 event

ldquoSEAFARMING TOMORROWrdquoTo be held from October 5-8 2010 in Porto Portugal

Hosted by CIMAR (CIIMAR and CCMAR)

AE2010 comprisesbull International Conferencebull Aquaculture Trade Exhibitionbull Farmersrsquo (industry) daybull Student Forumbull EU Forumbull Satellite Workshops amp Meetings

wwweasonlineorg

Aquaculture Europe - the annual meeting of the European Aquaculture Society

Join us in Porto

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 48: March | April 10 - International Aquafeed

1st - 5th March AQUACULTURE 2010 San Diego USA Contact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 923 34912Email mariostaelscarletbeWeb wwwmareventcom

2nd - 4th MarchACUI 2010 International Aquaculture Exhibition of Galicia Spain Contact David Barreiro

Tel +34 9864 88875 Fax +34 9815 52718Email infoacuiesWeb wwwacuies

3rd - 5th March Victam Asia 2010 Bangkok ThailandContact Andy West PO Box 411 Redhill RH1 6WE United Kingdom

Tel +44 1737 763501Fax + 44 1737 762996Email andrewwest733ntlworldcomWeb wwwvictamcom

26th - 26th MarchILDEX Vietnam Aquaculture Conference 2010 VietnamContact Sirapat Kettarn 12059 M 5 Bangduer Muang Patumthani 12000 Thailand

Tel +66 2978 2519Fax +66 2978 4548Email sksptmrkcom Web wwwsptmrkcom

20th - 23rd AprilGlobal Feed amp Food Congress Cancun Mexico Contact Mr Roger Gilbert 7 St Georges Terrace St James Square Cheltenham GL50 3PT United Kingdom

Tel +44 1242 267702Fax +44 1242 267701Email rogergilbertififorg Web wwwglobalfeed-foodcom

28th - 28th April GLOBAL GAP Press Conference Brussels BelgiumContact Claudia Meifert GLOBALGAP co FoodPLUS GmbH Spichernstr 55 50672 Koeln Germany

Tel +49 2215 799325 Fax +49 2215 799389Email meifertglobalgaporg Web wwwglobalgaporg

16th - 19th May Alltechrsquos 26th International Animal Health amp Nutrition Symposium Kentucky USAContact Lauren Ashley Pope 3031 Catnip Hill Pike USA

Tel +1 8598 812213 Fax +1 8598 873256Email symposiumalltechcom Web wwwalltechcom

19th - 20th May AQUACULTURE UK 2010 Aviemore Scotland Contact David Mack ASCOMBER Rosebank Ankerville Street Tain Ross amp Cromarty IV19 1BH Scotland

Tel +44 1862 892188Email infoaquacultureukcom Web wwwaquacultureukcom

23rd - 26th MayAustralasian Aquaculture 2010 International Conference and Trade Show Tasmania Australia Contact Sarah-Jane Day PO Box 370 Nelson Bay NSW 2317 Australia

Tel +61 4371 52234 Fax +61 2491 91044Email sarah-janeday aquacultureorgau Web www australian-aquacultureportalcom

16th - 18th June Offshore Mariculture Conference 2010 Dubrovnik Croatia Contact Isobel Roberts Mercator Media The Old Mill Lower Quay Fareham Hants PO16 0RA United Kingdom

Tel +44 1622 820622Email irobertsmercatormediacomWeb wwwoffshoremariculturecom

5th - 9th July The 9th International Congress of the Biology of Fish Barcelona Spain Contact Judith Garciacutea Mondial amp Cititravel Congresos SL C Rosselloacute 303 atc 1 08037 Barcelona Spain

Tel +34 9322 12955 Fax +34 9345 92059Email garciamondial-congresscomWeb httpsidcienciesuab es9FishBiologyCongress

8th - 10th July Indo Livestock 2010 Jakarta IndonesiaContact Devi Ardiatne PT Napindo Media Ashatama Jl Kelapa Sawit XIV Blok M1 No 1 Kompleks Billy amp Moon - Pondok Kelapa Jakarta 13450 Indonesia

Tel +62 2186 44756 Fax +62 2186 50963Email devinapindocom Web wwwindoaquaculturecom

6th - 8th October AQUACULTURE EUROPE 2010 Porto PortugalContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912Fax +32 9233 4912Email ae2010aquacultureccWeb wwwmareventcom

7th - 8th October GLOBALGAP Summit 2010 London United KingdomContact Nina Kretschmer co GLOBALGAP Foodplus GmbH Spichernstr55 D-50672 Cologne Germany

Tel +49 2215 7993693 Fax +49 2215 799389Email kretschmerglobalgaporg Web wwwsummit2010org

EVENTS 2011

28th February - 3rd MarchAQUACULTURE AMERICA 2011 New Orleans USAContact Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

26th - 29th MayAquarama 2011 Singapore Contact Doris Woo Linda Tan No3 Pickering Street ♯02-48 China Square Central Singapore 048660

Tel +65 65920889 Fax +65 64389060Email aquarama-sgubmcom Web wwwaquaramacomsg

6th - 10th June WORLD AQUACULTURE 2011 incl Giant Prawn 2011 Natal BrazilContact Mr Mario Stael MAREVENT Begijnengracht 40 9000 Gent Belgium

Tel +32 9233 4912 Fax +32 9233 4912Email mariostaelscarletbe Web wwwmareventcom

Events Key

= See our magazine at this show

bull = More information available

EVENTS

Is there an event that our readers need to know aboutEvents listings are free of charge and will appear in the printed magazine and onlineTo add your event to our listing contact Tuti Tan - tutitaquafeedcouk

Aquaculture UK 2010

Aquaculture UK 2008 was a sell-out and a great success

By general consensus the location and venue were welcomed by exhibitors and visitors

Many thanks for deliv-er ing a ver y successful exhibition and return to Aviemorerdquo said Alan Stewart - Director Landcatch after the event

As predicted Aquaculture UK 2008 was a tr u ly International event and has established itself as the UKs premier aquaculture exhibi-tion It attracted over 1000 visitors from 15 countries who came to see the latest products and innovations in the industry Alongside the exhibition Aquaculture UK 2008 ran a well attended conference which will be repeated in 2010

Already the majority of exhibitors have indicated that they will be returning in 2010 So space will be at a premium

Aquaculture UK 2010 offers exhibitors a valuable opportunity to launch new products meet the real buying decision makers and display and promote their products and services For visitors itrsquos an oppor tu-nity catch up on the latest technology and techniques meet old friends and make new ones in an accessible compact and pleasant setting Great networking and social opportunities are all part of the Aquaculture UK 2010 experience whether in the exhibition halls seminar rooms or relaxing afterwards in the resort restaurants and bars

46 | InternatIonal AquAFeed | March-april 2010

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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Page 49: March | April 10 - International Aquafeed

Leading technology ndash local solutions Whether for fish or crustaceans whether

sinking or floating feeds ndash for every product Buhler offers the perfect solution

from raw material processing mixing and extruding to drying Process expertise

combined with technology solutions made in Asia guarantees the production of

high-quality animal feeds But there is even more to it Buhler know-how also en-

ables customers to create the most cost- and energy-efficient process solution

from stand-alone machines to complete plants

The solution behind the solution

Buumlhler AG Feed amp Biomass CH-9240 Uzwil Switzerland T +41 71 955 11 11 F +41 71 955 28 96

fubuzbuhlergroupcom wwwbuhlergroupcom

BUZ_PE_ohneVictam_1209indd 1 25012010 084442

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